Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,070,2650GC53.8% ‑2.1 / 57.7 39intergenic (+62/+82)aroP‑I/PP_0928aromatic amino acid transport protein/K+‑dependent Na+/Ca+ exchanger‑like protein
Reads supporting (aligned to +/- strand):  ref base G (10/8);  new base C (10/11);  total (20/19)
Fisher's exact test for biased strand distribution p-value = 7.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.60e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCACCGGCAGTA  >  NC_002947/1070123‑1070411
                                                                                                                                              |                                                                                                                                                  
tcGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAGATCGAGCGCCGCgcgggcgg                                                                                                                                             <  2:1164171/150‑1 (MQ=255)
   gTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGACAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAGATCGAGCGCCGCCCGCGCGgcgc                                                                                                                                          <  1:2402900/150‑1 (MQ=255)
     aaTGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAGATCGAGCGCCGCGCGGGCGGCGCTc                                                                                                                                        <  1:41179/150‑1 (MQ=255)
      aTGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAGATCGAGCGCCGCgcgg                                                                                                                                                 >  1:1581569/1‑140 (MQ=255)
      aTGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAGATCGAGCGCCGCgcgg                                                                                                                                                 <  2:1581569/140‑1 (MQ=255)
      aTGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCg                                                                                                                                       <  2:1334933/150‑1 (MQ=255)
         cTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGAtc                                                                                                                                    <  1:184725/150‑1 (MQ=255)
          tGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAGATCGAGCGCCGCCCGCGCGgcgc                                                                                                                                          >  2:2442867/1‑143 (MQ=255)
          tGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAGATCGAGCGCCGCCCGCGCGgcgc                                                                                                                                          <  1:2442867/143‑1 (MQ=255)
               ccGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGAt                                                                                                                                     >  1:165545/1‑143 (MQ=255)
               ccGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGAt                                                                                                                                     <  2:165545/143‑1 (MQ=255)
                       gATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGCTTTCATCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGAtc                                                                                                                                    >  2:1942669/1‑136 (MQ=37)
                       gATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGCTTTCATCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGAtc                                                                                                                                    <  1:1942669/136‑1 (MQ=37)
                                    tgggtggCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAGATCGAGCGCCGCccgcgcgg                                                                                                                                             >  1:2416113/1‑114 (MQ=255)
                                    tgggtggCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAGATCGAGCGCCGCccgcgcgg                                                                                                                                             <  2:2416113/114‑1 (MQ=255)
                                            tttCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGa                                                                                                                                      <  2:571799/113‑1 (MQ=255)
                                            tttCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTGTCGGCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGa                                                                                                                                      >  1:571799/1‑113 (MQ=255)
                                            tttCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGCATGCCTTTAGGCTTTCATCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGa                                                                                                                                      >  2:1319739/1‑113 (MQ=18)
                                            tttCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGCATCCCTTTAGGCTTTCATCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGa                                                                                                                                      <  1:1319739/113‑1 (MQ=18)
                                                      aagaagGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTaaa                                                                                                              <  1:947076/127‑1 (MQ=255)
                                                      aagaagGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTaaa                                                                                                              >  2:947076/1‑127 (MQ=255)
                                                             cTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGAt                                                                                                                                     <  2:788633/97‑1 (MQ=255)
                                                             cTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGAt                                                                                                                                     >  1:788633/1‑97 (MQ=255)
                                                              tCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAg                                                                                                                              >  1:2239517/1‑103 (MQ=255)
                                                              tCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAg                                                                                                                              <  2:2239517/103‑1 (MQ=255)
                                                                      ccAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTcaca                                                                                                                               >  2:853428/1‑94 (MQ=255)
                                                                      ccAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTcaca                                                                                                                               <  1:853428/94‑1 (MQ=255)
                                                                        aaGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTc                                                                                                                                        >  1:2125077/1‑83 (MQ=255)
                                                                        aaGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTc                                                                                                                                        <  2:2125077/83‑1 (MQ=255)
                                                                                             gTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAgg                                                                                                                             <  1:1229905/73‑1 (MQ=255)
                                                                                             gTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAgg                                                                                                                             >  2:1229905/1‑73 (MQ=255)
                                                                                                                            tgtgAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAAtgct                                                           <  1:1635620/108‑1 (MQ=255)
                                                                                                                            tgtgAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAAtgct                                                           >  2:1635620/1‑108 (MQ=255)
                                                                                                                              tgAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCg               >  1:635360/1‑150 (MQ=255)
                                                                                                                                  aTCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCt                                                                                          <  2:2361166/71‑1 (MQ=21)
                                                                                                                                  aTCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCt                                                                                          >  1:2361166/1‑71 (MQ=21)
                                                                                                                                   tCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCAc          >  1:2274142/1‑150 (MQ=255)
                                                                                                                                    cGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCAcc         >  2:898494/1‑150 (MQ=255)
                                                                                                                                    cGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGCCTGGCGGCCCTGACGCGATCCCCTTCCATGCACCTCAATGCTGCCTCCGCCAAGGCCGCACAGTGGTGTACAACAACACCGTAGCCCGCAcc         >  2:1008392/1‑150 (MQ=38)
                                                                                                                                     gAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGcc                                                                                            <  2:876179/66‑1 (MQ=255)
                                                                                                                                     gAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGcc                                                                                            >  1:876179/1‑66 (MQ=255)
                                                                                                                                     gAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCa                                                                         >  1:53362/1‑85 (MQ=255)
                                                                                                                                     gAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCa                                                                         <  2:53362/85‑1 (MQ=255)
                                                                                                                                      aGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCACCgg       >  2:1235861/1‑150 (MQ=255)
                                                                                                                                           cgcgcgGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGc                <  1:463965/136‑1 (MQ=255)
                                                                                                                                           cgcgcgGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGc                >  2:463965/1‑136 (MQ=255)
                                                                                                                                           cgcgcgGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCACCGGCAGTa  >  2:1439332/1‑150 (MQ=255)
                                                                                                                                           cgcgcgGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCACCGGCAGTa  <  1:1008392/150‑1 (MQ=255)
                                                                                                                                           cgcgcgGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCACCGGCAGTa  <  1:898494/150‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
TCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCACCGGCAGTA  >  NC_002947/1070123‑1070411

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑