Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,951,3770GC37.1% 23.2 / 38.2 35intergenic (‑113/+48)oguA/PP_25858‑oxoguanine deaminase/alpha‑ketoglutaric semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (12/10);  new base C (7/6);  total (19/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-02
Rejected as consensus: Frequency below/above cutoff threshold.

GACGAGGAGCGTTTTTGCAGGGTTTGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAGGGTAGTT  >  NC_002947/2951229‑2951523
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gACGAGGAGCGTTTTTGCAGGGTTTGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTgg                                                                                                                                                   <  2:821486/150‑1 (MQ=255)
gACGAGGAGCGTTTTTGCAGGGTTTGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTgg                                                                                                                                                   <  2:272829/150‑1 (MQ=255)
gACGAGGAGCGTTTTTGCAGGGTTTGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTgg                                                                                                                                                   <  2:261198/150‑1 (MQ=255)
  cGAGGAGCGTTTTTGCAGGGTTTGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTcgcg                                                                                                                                                 >  1:172234/1‑150 (MQ=255)
        gCGTTTTTGCAGGGTTTGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTACCCGTGCCGGCCTCTTCGCGGCtcta                                                                                                                                           >  2:1164434/1‑147 (MQ=255)
                  aGGGTTTGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTCGCGGCTCTAGCCGCCAaga                                                                                                                                 <  2:172234/150‑1 (MQ=255)
                       ttGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTACCCGTGCCGGCCTCTTCGCGGCTCTAGCCGCCAAGAGGCCg                                                                                                                            <  1:1164434/150‑1 (MQ=38)
                        tGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTCGCGGCTCTAGCCGCCAAGAGGCCgg                                                                                                                           <  2:994999/150‑1 (MQ=255)
                                  aTGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCgg                                                                                                                           >  1:1122183/1‑140 (MQ=255)
                                  aTGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCgg                                                                                                                           <  2:1122183/140‑1 (MQ=255)
                                     gTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGCAGAGGCCGGCACGGGTTGCAGc                                                                                                              >  1:1192351/1‑150 (MQ=255)
                                        cAAGCCTGCGCAACACGACGCGGCGATTACCGATGTCATAGCTGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCTGGCCTCTTGGCGGCTAGAGCCGCTAAGCGGCCGGCACGGGTTGCAGCCAt                                                                                                           <  2:1015452/150‑1 (MQ=255)
                                            ccTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGaaa                                                                                                       <  1:3980/150‑1 (MQ=255)
                                               gcgcAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGAt                                                                                                    <  2:1057944/150‑1 (MQ=255)
                                                          aTCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTCGCGGCTCTAGCCGCCAaga                                                                                                                                 >  2:701103/1‑110 (MQ=38)
                                                          aTCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTCGCGGCTCTAGCCGCCAaga                                                                                                                                 <  1:701103/110‑1 (MQ=38)
                                                                      cGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGaaa                                                                                                       <  1:616321/124‑1 (MQ=255)
                                                                      cGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGaaa                                                                                                       >  2:616321/1‑124 (MQ=255)
                                                                      cGATGTCAGAGGCGCTCGGTATTGGAAGCAAGCGTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTcac                                                                             >  2:17626/1‑150 (MQ=255)
                                                                             aTAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTCGCGGCTCTAGCCGCCAaga                                                                                                                                 >  1:331703/1‑91 (MQ=25)
                                                                             aTAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTCGCGGCTCTAGCCGCCAaga                                                                                                                                 <  2:331703/91‑1 (MQ=25)
                                                                             aTAGGCACTCGGTATTGTAAGCAATCTTTTTACTGATTGTATACAGCTCGCTGTGCCCGTGCCGTCCTCTTGTCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTcc                                                                      >  2:61487/1‑150 (MQ=255)
                                                                                   gcTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAAc                                                                >  2:1003254/1‑150 (MQ=255)
                                                                                                    agcTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCgg                                               <  1:1003254/150‑1 (MQ=255)
                                                                                                              tGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGttt                                     >  1:943018/1‑150 (MQ=255)
                                                                                                                aTTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGt                                             >  2:688685/1‑140 (MQ=255)
                                                                                                                aTTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGt                                             <  1:688685/140‑1 (MQ=255)
                                                                                                                      taCAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGc                             >  1:65268/1‑150 (MQ=255)
                                                                                                                                  tGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCCCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGcagcag                 >  2:919770/1‑150 (MQ=255)
                                                                                                                                   gCCCGTGCCGGCCTCTTGGAGGCTAAGGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGt                <  2:1192351/150‑1 (MQ=255)
                                                                                                                                           cGGCCTCTTCGCGGCTCTAGCCGCCAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAgg        >  2:400116/1‑150 (MQ=255)
                                                                                                                                            ggCCTCTTCGCGGCTCTAGCCGCCAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAggg       >  2:1003530/1‑150 (MQ=39)
                                                                                                                                             gCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAGGGt      >  2:62755/1‑150 (MQ=255)
                                                                                                                                                tctTCGCGGCTCTAGCCGCCAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCaa                                <  2:1141579/121‑1 (MQ=38)
                                                                                                                                                tctTCGCGGCTCTAGCCGCCAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCaa                                >  1:1141579/1‑121 (MQ=38)
                                                                                                                                                tctTCGCGGCTCTAGCCGCCAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAGGGTAGt   >  1:1237337/1‑150 (MQ=39)
                                                                                                                                                 ctTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAGGGTAGtt  <  1:17626/150‑1 (MQ=255)
                                                                                                                                                  ttGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAgg        >  2:1318929/1‑143 (MQ=255)
                                                                                                                                                  ttGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAgg        <  1:1318929/143‑1 (MQ=255)
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GACGAGGAGCGTTTTTGCAGGGTTTGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAGGGTAGTT  >  NC_002947/2951229‑2951523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑