Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,741,273	1	.	C	61.5%	78.5 / 50.9	39	intergenic (+464/+288)	gltA/yeiW	citrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base . (5/10);  new base C (13/11);  total (18/21)
Fisher's exact test for biased strand distribution p-value = 3.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GTAAGTCGGCTCTTCTTTCAGGTTACGGGTCAGGCTGCCATCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑AA‑CCTCC‑GCCAAC‑TGCATTCGTGAA‑CCAAGCAACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGC  >  NC_002947/4741127‑4741401                                                                                                                                                    |                                                                                                                                     gTAAGTCGGCTCTTCTTTCAGGTTACGGGTCAGGCTGCCATCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGAGGGAGTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTgagcag                                                                                                                                    >  1:420575/1‑147 (MQ=255)                                         tCCGCCTCGTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCGCCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCT‑GCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCg                                                                                        <  1:120711/150‑1 (MQ=255)                                            gCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGac                                                                                     >  1:273518/1‑150 (MQ=255)                                                       ggTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCT‑GCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTg                                                                          >  1:465956/1‑150 (MQ=255)                                                        gTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGc                                                                         >  1:1101092/1‑150 (MQ=255)                                                        gTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGc                                                                         >  1:767749/1‑150 (MQ=255)                                                                      caCGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGacac                                                                                   <  2:1332138/126‑1 (MQ=255)                                                                      caCGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGacac                                                                                   >  1:1332138/1‑126 (MQ=255)                                                                      caCGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCtt                                                           >  1:645596/1‑150 (MQ=255)                                                                      caCGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCT‑GCGACT‑TGCATTCGTGAATCCACGC‑ACAGGCCCCGGACACGTCCTCCGGCAGCGCCTCGACCtt                                                           >  2:911075/1‑150 (MQ=255)                                                                      caCGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTC‑TGCGACTTGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCtt                                                           <  2:711423/150‑1 (MQ=255)                                                                      caCGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTC‑TGCGACTTGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCCCCTGCAGCGCCTCGACCtt                                                           >  1:236870/1‑150 (MQ=255)                                                                         ggAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGc                                                        <  2:1153442/150‑1 (MQ=255)                                                                                        cACCGGCCATGGCGTATTGAACTTGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTC‑TGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGAt                                         <  2:137115/150‑1 (MQ=255)                                                                                          ccGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCT‑GCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCa                                       >  1:556348/1‑150 (MQ=255)                                                                                            ggCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCT‑GCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACg                                     <  1:1220413/150‑1 (MQ=255)                                                                                            ggCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTC‑TGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACg                                     <  1:440302/150‑1 (MQ=255)                                                                                                        ttGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCT‑GCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCCCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGa                         <  2:236870/150‑1 (MQ=255)                                                                                                           aaCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAg                      >  2:1161518/1‑150 (MQ=255)                                                                                                           aaCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCT‑GCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAg                      <  1:111490/150‑1 (MQ=255)                                                                                                                ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCtt                 <  2:1101092/150‑1 (MQ=255)                                                                                                                ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCT‑GCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGc                                                        <  1:1140588/111‑1 (MQ=255)                                                                                                                ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGc                                                        >  2:1140588/1‑111 (MQ=255)                                                                                                                  tataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGt               >  1:788248/1‑150 (MQ=255)                                                                                                                  tataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCT‑GCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGt               >  1:1201008/1‑150 (MQ=255)                                                                                                                   ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTa              <  2:788248/150‑1 (MQ=255)                                                                                                                      ggTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACtt           <  2:273518/150‑1 (MQ=255)                                                                                                                      ggTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACtt           <  1:320406/150‑1 (MQ=255)                                                                                                                       gTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCT‑GCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTGGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTg          <  1:911075/150‑1 (MQ=255)                                                                                                                        tCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTg                                                          >  1:749933/1‑101 (MQ=255)                                                                                                                        tCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCT‑GCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTg                                                          <  2:749933/101‑1 (MQ=255)                                                                                                                          cAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGAc       >  2:595047/1‑150 (MQ=255)                                                                                                                           aGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCT‑GCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTcaca                               >  2:591514/1‑125 (MQ=255)                                                                                                                           aGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTcaca                               <  1:591514/125‑1 (MQ=255)                                                                                                                               ctGGTCACCTTCGTGCTTGAGCAG‑CTTCT‑GCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGc  <  2:767749/150‑1 (MQ=255)                                                                                                                               ctGGTCACCTTCGTGCTTGAG‑CAGCTTCT‑GCGACT‑TGCATTCGTGAA‑TCCCGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGc  <  2:465956/150‑1 (MQ=255)                                                                                                                                   tCACCTTCGTGCTTGAG‑CAGCTTCT‑GCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGa        >  1:1200976/1‑140 (MQ=255)                                                                                                                                   tCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGa        <  2:1200976/140‑1 (MQ=255)                                                                                                                                     aCCTTCGTGCTTGAGCAG‑CTTCT‑GCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTcaca                               >  1:1032488/1‑115 (MQ=255)                                                                                                                                     aCCTTCGTGCTTGAG‑CAGCTTCT‑GCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTcaca                               <  2:1032488/115‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                     GTAAGTCGGCTCTTCTTTCAGGTTACGGGTCAGGCTGCCATCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑AA‑CCTCC‑GCCAAC‑TGCATTCGTGAA‑CCAAGCAACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGC  >  NC_002947/4741127‑4741401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑