Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	NC_002947	5,911,030	Δ6 bp	16.9%	coding (1179‑1184/1359 nt)	spuB ←	glutamylpolyamine synthetase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_002947	= 5911029	92 (0.800)	18 (0.160)	17/234	NT	16.9%	coding (1185/1359 nt)	spuB	glutamylpolyamine synthetase
	?	NC_002947	5911036 =	89 (0.810)					coding (1178/1359 nt)	spuB	glutamylpolyamine synthetase

CACGCTCCCACGAACTGATGACGCGCTTGAAGTTTTCATGCTCGGCGCGCTTGGTAGCGACATACCCGGTGATGAATTTCTTGCCCAGGTACTGCACCAGCGCACGGCTGTTTTCCATGCGTTCCAAGGCGTCCTCGATGGTCAGGGGCAGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_002947/5910878‑5911029 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gcaggTTGCGGCGTTCGTAGCCACGGCCCTGCACTGGCGCGCTGGCCTCGATACCTTCGACCATGCCGATGTAGCCACACAGTAGGCTGGCGGCGATGGCCAGGTACGGGTTGGCGTCGGCGCCCGGCAGGCGGTTCTCGACGCGCCGGCTTTG  >  NC_002947/5911036‑5911184                                                                                                                                                                                                                                                                                                               CACGCTCCCACGAACTGATGACGCGCTTGAAGTTTTCATGCTCGGCGCGCTTGGTAGCGACATACCCGGTGATGAATTTCTTGCCCAGGTACTGCACCAGCGCACGGCTGTTTTCCATGCGTTCCAAGGCGTCCTCGATGGTCAGGGGCA                                                                                                                                                         >  2:2618510/1‑150  ACGTTCCCACGAACGGATGACGCGCTTGAAGTTTTCATAATCGCCGCTCTTGGTAGAGACATACCCGGTGATGAATTTCTTGCCCAGGTACTGCACCAGCGCACGGCTGTTTTCCATGCGTTCCAAGGCGTCCTCGATGGTCAGGGGCAG                                                                                                                                                        >  2:1642974/1‑150  ACGCTCCCACGAACTGATGACGCGCTTGAAGTTTTCATGCTCGGCGCGCTTGGTAGCGACATACCCGGTGATGAATTTCTTGCCCAGGTACTGCACCAGCGCACGGCTGTTTTCCATGCGTTCCAAGGCGTCCTCGATGGTCAGGGGCAG                                                                                                                                                        <  2:2624244/150‑1                       GCGCTTGAAGTTTTCATGCTCGGCGCGCTTGGTAGCGACATACCCGGTGATGAATTTCTTGCCCAGGTGGTGCACCAGCGCACGGCTGTTTTCCATGCGTTCCAAGGCGTCCTCGATGGTCAGGGGCAGGTTGCGGCGTTCGTAGCCACG                                                                                                                                   >  1:2518361/1‑150                               AGTTTTCATGCTCGGCGCGCTTGGTAGCGACATACCCGGTGATGAATTTCTTGCCCAGGTACTGCACCAGCGCACGGCTGTTTTCCATGCGTTCCAAGGCGTCCTCGATGGTCAGGGGCAGGTTGCGGCGTTCGTAGCCACGGCCCTGCA                                                                                                                           >  2:1095795/1‑150                                 TTTTCATGCTCGGCGCGCTTGGTAGCGACATACCCGGTGATGAATTTCTTGCCCAGGTACTGCACCAGCGCACGGCTGTTTTCCATGCGTTCCAAGGCGTCCTCGATGGTCAGGGGC                                                                                                                                                          <  1:2670724/117‑1                                 TTTTCATGCTCGGCGCGCTTGGTAGCGACATACCCGGTGATGAATTTCTTGCCCAGGTACTGCACCAGCGCACGGCTGTTTTCCATGCGTTCCAAGGCGTCCTCGATGGTCAGGGGC                                                                                                                                                          >  2:2670724/1‑117                                  TTTCATGCTCGGCGCGCTTGGTAGCGACATACCCGGTGATGAATTTCTTGCCCAGGTACTGCACCAGCGCACGGCTGTTTTCCATGCGTTCCAAGGCGTCCTCGATGGTCAGGGGCAGGTTGCGGCGTTCGTAGCCACGGCCCTGCACTG                                                                                                                        <  2:762316/150‑1                                        GCTCGGCGCGCTTGGTAGCGACATACCCGGTGATGAATTTCTTGCCCAGGTACTGCACCAGCGCACGGCTGTTTTCCATGCGTTCCAAGGCGTCCTCGATGGTCAGGGGCAGGTTGCGGCGTTCGTAGCCACGGCCCTGCACTGGCGCGC                                                                                                                  >  1:2725655/1‑150                                                                    GGTGATGAATTTCTTGCCCAGGTACTGCACCAGCGCACGGCTGTTTTCCATGCGTTCCAAGGCGTCCTCGATGGTCAGGGGCAGGTTGCGGCGTTCGTAGCCACGGCCCTGCACTGGCGCGCTGGCCTCGATACCTTCGACCATGCCGAT                                                                                      <  2:1139133/150‑1                                                                                      CAGGTACTGCACCAGCGCACGGCTGTTTTCCATGCGTTCCAAGGCGTCCTCGATGGTCAGGGGCAGGTTGCGGCGTTCGTAGCCACGGCCCTG                                                                                                                             >  1:1451949/1‑93                                                                                      CAGGTACTGCACCAGCGCACGGCTGTTTTCCATGCGTTCCAAGGCGTCCTCGATGGTCAGGGGCAGGTTGCGGCGTTCGTAGCCACGGCCCTG                                                                                                                             <  2:1451949/93‑1                                                                                         GTACTGCACCAGCGCACGGCTGTTTTCCATGCGTTCCAAGGCGTCCTCGATGGTCAGGGGCAGGTTGCGGCGTTCGTAGCCACGGCCCTGCACTGGCGCGCTGGCCTCGATACCTTCGACCATGCCGATGTAGCCACACAGTAGGCTGGC                                                                 >  1:1819183/1‑150                                                                                                ACCAGCGCACGGCTGTTTTCCATGCGTTCCAAGGCGTCCTCGATGGTCAGGGGCAGGTTGCGGCGTTCGTAGCCACGGCCCTGCACTGGCGCGCTGGCCTCGATACCTTCGACCATGCCGATGTAGCCACACAGTAGGCTGGCGGCGATG                                                          <  2:2518361/150‑1                                                                                                     CGCACGGCTGTTTTCCATGCGTTCCAAGGCGTCCTCGATGGTCAGGGGCAGGTTGCGGCGTTCGTAGCCACGGCCCTGCACTGGCGCGCTGGCCTCGATACCTTCGACCATGCCGATGTAGCCACACAGTAGGCTGGCGGCGATGGCCAG                                                     <  1:1095795/150‑1                                                                                                            CTGTTTTCCATGCGTTCCAAGGCGTCCTCGATGGTCAGGGGCAGGTTGCGGCGTTCGTAGCCACGGCCCTGCACTGGCGCGCTGGCCTCGATACCTTCGACCATGCCGATGTAGCCACACAGTAGGCTGGCGGCGATGGCCAGGTACGGG                                              >  1:1709957/1‑150                                                                                                                      TGCGTTCCAAGGCGTCCTCGATGGTCAGGGGCAGGTTGCGGCGTTCGTAGCCACGGCCCTGCACTGGCGCGCTGGCCTCGATACCTTCGACCATGCCGATGTAGCCACACAGTAGGCTGGCGGCGATGGCCAGGTACGGGTTGGCGTCGG                                    >  2:943378/1‑150                                                                                                                      TGCGTTCCAAGGCGTCCTCGATGGTCAGGGGCAGGTTGCGGCGTTCGTAGCCACGGCCATGCACTGGCGCGCTGGCCTCGATACCTTCGACCATGCCGATGTAGCCACACAGTAGGCTGGCGGCGATGGCCAGGTACGGGTTGGCGTCGG                                    >  2:865290/1‑150                                                                                                                       GCGTTCCAAGGCGTCCTCGATGGTCAGGGGCAGGTTGCGGCGTTCGTAGCCACGGCCCTGCACTGGCGCGCTGGCCTCGATACCTTCGACCATGCCGATGTAGCCACACAGTAGGCTGGCGGCGATGG                                                         <  1:2806336/128‑1                                                                                                                       GCGTTCCAAGGCGTCCTCGATGGTCAGGGGCAGGTTGCGGCGTTCGTAGCCACGGCCCTGCACTGGCGCGCTGGCCTCGATACCTTCGACCATGCCGATGTAGCCACACAGTAGGCTGGCGGCGATGG                                                         >  2:2806336/1‑128                                                                                                                                   GTCCTCGATGGTCAGGGGCAGGTTGCGGCGTTCGTAGCCACGGCCCTGCACTGGCGCGCTGGCCTCGATACCTTCGACCATGCCGATGTAGCCACACAGTAGGCTGGCGGCGATGGCCAGGTACGGGTTGGCGTCGGCGCCCGGCAGGCG                       >  1:1372027/1‑150                                                                                                                                      CTCGATGGTCAGGGGCAGGTTGCGGCGTTCGTAGCCACGGCCCTGCACTGGCGCGCTGGCCTCGATACCTTCGACCATGCCGATGTAGCCACACAGTAGGCTGGCGGCGATGGCCAGGTACGGGTTGGCGTCGGCGCCCGGCAGGCGGTT                    >  2:2685533/1‑150                                                                                                                                            GGTCAGGGGCAGGTTGCGGCGTTCGTAGCCACGGCCCTGCACTGGCGCGCTGGCCTCGATACCTTCGCGTTCGCCGATGTAGCCACACAGTAGGCTGGCGGCGATGGCCAGGTACGGGTTGGCGTCGGCGCCCGGCAGGCGGTTCTCGAC              <  1:1593623/150‑1                                                                                                                                                    GCAGGTTGCGGCGTTCGTAGCCACGGCCCTGCACTGGCGCGCTGGCCTCGATACCTTCGACCATGCCGATGTAGCCACACAGTAGGCTGGCGGCGATGGCCAGGTACGGGTTGGCGTCGGCGCCCGGCAGGCGGTTCTCGACGCGCCGGC      >  2:705200/1‑150                                                                                                                                                     CAGGTTGCGGCGTTCGTAGCCACGGCCCTGCACTGGCGCGCTGGCCTCGATACCTTCGACCATGCCGATGTAGCCACACAGTAGGCTGGCGGCGATGGCCAGGTACGGGTTGGCGTCGGCGCCCGGCAGGCGGTTCTCGACGCGCCGGCT     <  1:979784/150‑1                                                                                                                                                      AGGTTGCGGCGTTCGTAGCCACGGCCCTGCACTGGCGCGCTGGCCTCGATACCTTCGACCATGCCGATGTAGCCACACAGTAGGCTGGCGGCGATGGCCAGGTACGGGTTGGCGTCGGCGCCCGGCAGGCGGTTCTCGACGCGCCGGCTT    <  1:1583489/150‑1                                                                                                                                                        GTTGCGGCGTTCGTAGCCACGGCCCTGCACTGGCGCGCTGGCCTCGCTACCTTCGACCATGCCGATGTAACCAGGCAGTAGGCTGGCGGCGATGGCCAGGTACGGGTGTGCGGCGGCGCCCGGCGGGCGGTTCTAGACGCGCCGGCTTTG  >  1:691166/1‑150                                                                                                                                                                                                                                                                                                               CACGCTCCCACGAACTGATGACGCGCTTGAAGTTTTCATGCTCGGCGCGCTTGGTAGCGACATACCCGGTGATGAATTTCTTGCCCAGGTACTGCACCAGCGCACGGCTGTTTTCCATGCGTTCCAAGGCGTCCTCGATGGTCAGGGGCAGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_002947/5910878‑5911029 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gcaggTTGCGGCGTTCGTAGCCACGGCCCTGCACTGGCGCGCTGGCCTCGATACCTTCGACCATGCCGATGTAGCCACACAGTAGGCTGGCGGCGATGGCCAGGTACGGGTTGGCGTCGGCGCCCGGCAGGCGGTTCTCGACGCGCCGGCTTTG  >  NC_002947/5911036‑5911184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.