| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,808,041 | T→C | 8.1% | intergenic (+120/+35) | mrcA → / ← maeB | penicillin‑insensitive transglycosylase/penicillin‑sensitive transpeptidase/malic enzyme B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,808,041 | 0 | T | C | 8.1% | 172.5 / 4.4 | 62 | intergenic (+120/+35) | mrcA/maeB | penicillin‑insensitive transglycosylase/penicillin‑sensitive transpeptidase/malic enzyme B |
| Reads supporting (aligned to +/- strand): ref base T (26/30); new base C (3/2); total (29/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01 | |||||||||||
ATGAAGCGACGCCGATGGATCTGTTCTAAGGCAGGGCGCCTGACTCAGGTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACAC‑CTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCTTGGCCACAGCATCGGAAACGACGGT > NC_002947/5807893‑5808186 | aTGAAGCGACGCCGATGGATCTGTTCTAAGGCAGGGCGCCTGACTCAGGTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACAC‑CTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATg < 1:1842626/150‑1 (MQ=255) aCGCCGATGGATCTGTTCTAAGGCAGGGCGCCTGACTCAGGTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACAC‑CTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCgg < 2:387619/150‑1 (MQ=255) gCCGATGGATCTGTTCTAAGGCAGGGCGCCTGACTCAGGTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACAC‑CTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCgggg < 1:1544877/150‑1 (MQ=255) gATGGATCTGTTCTAAGGCAGGGCGCCTGACTCAGGTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACAC‑CTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCtt < 1:1840547/150‑1 (MQ=255) aTGGATCTGTTCTAAGGCAGGGCGCCTGACTCAGGTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACAC‑CTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCttt < 2:88820/150‑1 (MQ=255) aTGGATCTGTTCTAAGGCAGGGCGCCTGACTCAGGTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACAC‑CTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCttt < 1:3040540/150‑1 (MQ=255) tGGATCTGTTCTAAGGCAGGGCGCCTGACTCAGGTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACAC‑CTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCGCATGAGCCGGGGCtttt < 1:60799/150‑1 (MQ=255) aTCTGTTCTAAGGCAGGGCGCCTGACTCAGGTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACAC‑CTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTa > 1:1207698/1‑150 (MQ=255) ttCTAAGGCAGGGCGCCTGACTCAGGTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACAC‑CTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCg > 1:2055561/1‑150 (MQ=255) gTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACAC‑CTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCGCATGAGCCGGGGCtttt < 1:1552666/117‑1 (MQ=38) gTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACAC‑CTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCGCATGAGCCGGGGCtttt > 2:1552666/1‑117 (MQ=38) ttttGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACAC‑CTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACt > 1:2315114/1‑150 (MQ=255) gTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACAC‑CTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATcc > 2:2141546/1‑150 (MQ=255) gcTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACAC‑CTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTg < 2:1207698/150‑1 (MQ=255) tCGATCTGATAGGCGCCACAAGTGCCCTGACCAACAC‑CTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGt > 1:1124072/1‑150 (MQ=255) cGATCTGATAGGCGCCACAAGTGCCCTGACCAACTCTCAAGCA‑CAAGAAGGCATTCCCAAGCCCCATAAAAAAAAACCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTc < 1:2215558/150‑1 (MQ=255) gATCTGATAGGCGCCACAAGTGCCCTGACCAACAC‑CTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCgg > 2:1025176/1‑93 (MQ=255) gATCTGATAGGCGCCACAAGTGCCCTGACCAACAC‑CTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCgg < 1:1025176/93‑1 (MQ=255) gATCTGATAGGCGCCACAAGTGCCCTGACCAACAC‑CTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATg > 2:1575542/1‑105 (MQ=255) gATCTGATAGGCGCCACAAGTGCCCTGACCAACAC‑CTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATg < 1:1575542/105‑1 (MQ=255) ggCGCCACAAGTGCCCTGACCAACAC‑CTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAAt < 2:1124072/150‑1 (MQ=255) cacaAGTGCCCTGACCAACAC‑CTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTc > 1:1123681/1‑150 (MQ=255) aaGTGCCCTGACCAACAC‑CTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCt < 2:1467119/80‑1 (MQ=255) aaGTGCCCTGACCAACAC‑CTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCt > 1:1467119/1‑80 (MQ=255) aaGTGCCCTGACCAACAC‑CTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGa < 1:478345/150‑1 (MQ=255) gTGCCCTGACCAACAC‑CTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAacac < 2:1470476/114‑1 (MQ=255) gTGCCCTGACCAACAC‑CTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAacac > 1:1470476/1‑114 (MQ=255) gCCCTGACCAACAC‑CTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAg < 2:1676052/150‑1 (MQ=255) cccTGACCAACAC‑CTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTtg > 1:1050147/1‑90 (MQ=255) cccTGACCAACAC‑CTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTtg < 2:1050147/90‑1 (MQ=255) ccTGACCAACAC‑CTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGtt < 1:243265/104‑1 (MQ=255) ccTGACCAACAC‑CTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGtt > 2:243265/1‑104 (MQ=255) ccTGACCAACAC‑CTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACg > 2:2686846/1‑120 (MQ=255) ccTGACCAACAC‑CTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACg < 1:2686846/120‑1 (MQ=255) cTGACCAACAC‑CTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAACCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCa < 2:2055561/150‑1 (MQ=255) aaCAC‑CTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAg > 1:2477540/1‑150 (MQ=255) acac‑CTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCgg > 2:3013269/1‑123 (MQ=255) acac‑CTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCgg < 1:3013269/123‑1 (MQ=255) acac‑CTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTaa < 1:2672691/127‑1 (MQ=255) acac‑CTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTaa > 2:2672691/1‑127 (MQ=255) cacaGTCCCAAGCCCCATAAACAAGCCCCGGCTCAGGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGAAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATgg < 2:1123681/150‑1 (MQ=255) ccAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCgg < 1:365937/99‑1 (MQ=255) ccAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCgg > 2:365937/1‑99 (MQ=255) cAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCTTg > 2:2169132/1‑150 (MQ=255) aaaaaGCCCCGGCTCGCATGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCTTGGCCACAGCATc > 1:714991/1‑150 (MQ=39) aaaaGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATc > 1:1324506/1‑71 (MQ=255) aaaaGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATc < 2:1324506/71‑1 (MQ=255) aaaaGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCTTGGCCACAGCATCg > 1:2529554/1‑150 (MQ=255) gCCCCGGCTCGCATGAGCCGGCGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATTCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGCGTAGCTACGCCGGATTGGATAGCAGTCTTGGCCACAGCTTCGGcaa > 2:526021/1‑150 (MQ=255) gCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAgg > 1:698776/1‑104 (MQ=255) gCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAgg < 2:698776/104‑1 (MQ=255) ccccGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCTTGGCCACAGCATCGGAAac < 2:2529554/150‑1 (MQ=255) cccGGCTCATGCGAGCCGGGGCTTTTTTATGGGGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCTTGGCCACAGCATCGGAAacg > 1:280791/1‑150 (MQ=255) cccGGCTCATGCGAGCCGGGGCTTTTTAATATCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCTTGGCCACAGCATCGGAAacg > 1:2094581/1‑150 (MQ=255) cGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCTTGGCCACAGCATCGGAAacgac < 1:2169132/150‑1 (MQ=255) cGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGATTCGGATaa < 2:2123137/94‑1 (MQ=255) cGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGATTCGGATaa > 1:2123137/1‑94 (MQ=255) cTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCTTGGCCACAGCATCGGAAACGACGGt < 2:2315114/150‑1 (MQ=255) aTGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGc < 2:1695835/99‑1 (MQ=255) aTGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGc > 1:1695835/1‑99 (MQ=255) aTGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGcc < 2:345516/105‑1 (MQ=255) aTGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGcc > 1:345516/1‑105 (MQ=255) | ATGAAGCGACGCCGATGGATCTGTTCTAAGGCAGGGCGCCTGACTCAGGTTTTGCGTGGGCGCTCGATCTGATAGGCGCCACAAGTGCCCTGACCAACAC‑CTTGCAGCCCTGACACATTCCCAAGCCCCATAAAAAAGCCCCGGCTCATGCGAGCCGGGGCTTTTTTATGTCGCTTGCTGCAATCAGCCGTTGAACACTTCATCCACGCTGGTCAGCGGGTAATGCTTCGGATAAGGCAGGGTAGCCACGCCGGATTCGATGGCAGCCTTGGCCACAGCATCGGAAACGACGGT > NC_002947/5807893‑5808186 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |