Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 104,876 G→C 5.9% P345P (CCG→CCC)  PP_0099 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947104,8760GC 5.9% 346.4 / 6.9 110P345P (CCG→CCC) PP_0099hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (48/53);  new base C (2/5);  total (50/60)
Fisher's exact test for biased strand distribution p-value = 4.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 115 total aligned reads displayed.

GTATGACAGCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCA‑ATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGTTCCCGCCTTTTT  >  NC_002947/104732‑105024
                                                                                                                                                |                                                                                                                                                     
gTATGACAGCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTgg                                                                                                                                                  <  1:798842/150‑1 (MQ=255)
     aCAGCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGa                                                                                                                                             <  1:2882606/150‑1 (MQ=255)
       aGCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATg                                                                                                                                           >  2:3012492/1‑150 (MQ=255)
       aGCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCCGGTGGGCaaaac                                                                                                                                           <  1:1540357/150‑5 (MQ=255)
        gCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGc                                                                                                                                                >  1:2501015/1‑144 (MQ=255)
        gCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGc                                                                                                                                                <  2:2501015/144‑1 (MQ=255)
         ccGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTCCAGGCCTAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGACGAGGATGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGgc                                                                                                                                         >  1:1781621/1‑150 (MQ=255)
         ccGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGgc                                                                                                                                         >  1:775892/1‑150 (MQ=255)
             ttCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTg                                                                                                                                     <  1:2099303/150‑1 (MQ=255)
                 gCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCt                                                                                                                                 <  2:2446172/150‑1 (MQ=255)
                 gCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCt                                                                                                                                 <  1:1709662/150‑1 (MQ=255)
                   gTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGa                                                                                                                               <  2:2675797/150‑1 (MQ=255)
                    tGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGAc                                                                                                                              <  2:915001/150‑1 (MQ=255)
                     gcgcATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACa                                                                                                                             <  1:856851/150‑1 (MQ=255)
                       gcATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAAGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATg                                                                                                                           >  2:1177496/1‑150 (MQ=255)
                          tGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAac                                                                                                                        <  1:319654/150‑1 (MQ=255)
                          tGCGTGGTGAAGGAGTATTTGCCGAGTTGCCGGTCGTGCGGTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAac                                                                                                                        <  2:1781621/150‑1 (MQ=255)
                            cGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTc                                                                                                                                  <  2:1416962/138‑1 (MQ=255)
                                 tgAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAaccccaccc                                                                                                                 <  2:1559604/150‑1 (MQ=255)
                                  gAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCCGGTGGGCaaa                                                                                                                                             <  1:2722197/121‑3 (MQ=255)
                                  gAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCCGGTGGGCaaa                                                                                                                                             >  2:2722197/1‑119 (MQ=255)
                                   aaGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGCCGCTGTTCTGACATGAACCCCAccccc                                                                                                               >  1:1542119/1‑150 (MQ=255)
                                      gAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGa                                                                                                                               >  1:1042164/1‑131 (MQ=255)
                                      gAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGa                                                                                                                               <  2:1042164/131‑1 (MQ=255)
                                      gAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCcac                                                                                                            >  1:126059/1‑150 (MQ=255)
                                         tATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAgg                                                                                                         >  2:407830/1‑150 (MQ=255)
                                         tATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGCAACAGGAGTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAgg                                                                                                         >  1:1907319/1‑150 (MQ=255)
                                            ttGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGCGCAGATGGCGCTGTTCTTACATGAACTCCTCCCCCCACAggggg                                                                                                      <  2:1572687/150‑1 (MQ=255)
                                                cGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTgc                                                                                                  >  2:215913/1‑150 (MQ=255)
                                                cGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTACCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTgc                                                                                                  >  1:1261282/1‑150 (MQ=255)
                                                 gAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTgcg                                                                                                 >  2:74551/1‑150 (MQ=255)
                                                       cTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGc                                                                                           >  2:260949/1‑150 (MQ=255)
                                                        tGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCgg                                                                                                                                                      <  1:2397066/90‑1 (MQ=255)
                                                        tGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCgg                                                                                                                                                      >  2:2397066/1‑90 (MQ=255)
                                                        tGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGAc                                                                                                                              >  2:1479296/1‑114 (MQ=255)
                                                        tGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGAc                                                                                                                              <  1:1479296/114‑1 (MQ=255)
                                                        tGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCt                                                                                          >  2:2983346/1‑150 (MQ=255)
                                                            gcAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCAccccc                                                                                                               <  2:1537604/125‑1 (MQ=255)
                                                            gcAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCAccccc                                                                                                               >  1:1537604/1‑125 (MQ=255)
                                                            gcAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTcaca                                                                                      <  2:615316/150‑1 (MQ=255)
                                                               gcgcTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACg                                                                                   <  1:407830/150‑1 (MQ=255)
                                                                 gcTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTca                                                                                        <  2:2593074/143‑1 (MQ=255)
                                                                 gcTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTca                                                                                        >  1:2593074/1‑143 (MQ=255)
                                                                 gcTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCa                                                                                 >  1:1732291/1‑150 (MQ=255)
                                                                  cTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCTGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCaa                                                                                <  1:66591/150‑1 (MQ=255)
                                                                  cTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGa                                                                                                                               >  2:2333882/1‑103 (MQ=255)
                                                                  cTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGa                                                                                                                               <  1:2333882/103‑1 (MQ=255)
                                                                   ttcgCCAAGGCAATGAAACGGCTGCATTTCGACGGGCCCGAGGCGCATGCGTTTGATTGCTCGCCGTTTTCCCCATCTGGTGCCCAGCTGGCGCTGTTCTGACATGACCCCCACCCTCCACATGGTGCTGCGCCTTGCTCACAACGCAAc                                                                               <  1:625282/147‑1 (MQ=255)
                                                                      gCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACtaa                                                                            >  2:2278520/1‑150 (MQ=255)
                                                                      gCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACtaa                                                                            >  1:757357/1‑150 (MQ=255)
                                                                      gCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACtaa                                                                            <  2:757357/150‑1 (MQ=255)
                                                                       ccAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCa                                                                                               >  2:719105/1‑130 (MQ=255)
                                                                        cAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGc                                                                                           >  2:157603/1‑133 (MQ=255)
                                                                        cAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGc                                                                                           <  1:157603/133‑1 (MQ=255)
                                                                         aaGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCAt                                                                                              <  1:1254198/129‑1 (MQ=255)
                                                                         aaGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCAt                                                                                              >  2:1254198/1‑129 (MQ=255)
                                                                          aGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATaac                                                                        <  1:1117579/150‑1 (MQ=255)
                                                                              aaTGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTggg                                                                                                                                                 >  2:2987065/1‑73 (MQ=255)
                                                                              aaTGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTggg                                                                                                                                                 <  1:2987065/73‑1 (MQ=255)
                                                                                 gAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCa‑at                                                                >  1:242912/1‑150 (MQ=255)
                                                                                   aaCGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCa‑ataa                                                              >  1:252396/1‑150 (MQ=255)
                                                                                     cGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCA‑ATAAtt                                                            <  1:2278520/150‑1 (MQ=255)
                                                                                     cGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCA‑ATAAtt                                                            <  1:260949/150‑1 (MQ=255)
                                                                                      ggCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCA‑ATAATTg                                                           <  1:215913/150‑1 (MQ=255)
                                                                                       gCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCgg                                                                                                                                                      <  1:153594/59‑1 (MQ=255)
                                                                                       gCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCgg                                                                                                                                                      >  2:153594/1‑59 (MQ=255)
                                                                                       gCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTgg                                                                                                                                                  >  1:931012/1‑63 (MQ=255)
                                                                                        cTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCA‑ATAATTgcg                                                         <  2:158649/150‑1 (MQ=255)
                                                                                        cTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCA‑ATAATTgcg                                                         <  2:1732291/150‑1 (MQ=255)
                                                                                         tGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCA‑ATAATTgcgc                                                        <  2:252396/150‑1 (MQ=255)
                                                                                           gATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCA‑ATAATTGCGCTa                                                      >  1:301749/1‑150 (MQ=255)
                                                                                            aTTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCA‑ATAATTGCGCTaa                                                     <  2:1542119/150‑1 (MQ=255)
                                                                                             tttCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCA‑ATAATTGCGCTAAt                                                    <  2:1907319/150‑1 (MQ=255)
                                                                                                cGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCGCGCCCGCTAGGCAGATGTCGCTGTTCTCACCCGAGCCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCA‑ATAATTGCGCTAATACa                                                 <  1:2983346/150‑1 (MQ=255)
                                                                                                      gcgcGAGGCGCAGGAGCTGGATTGCTCGGCGTTCTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCA‑ATAATTGCGCTAATACATATTgg                                           <  1:655611/150‑1 (MQ=255)
                                                                                                       cgcgAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAgg                                                                                                         >  1:296731/1‑88 (MQ=255)
                                                                                                        gcgAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCA‑ATAATTgc                                                          >  2:1901594/1‑133 (MQ=255)
                                                                                                        gcgAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCA‑ATAATTgc                                                          <  1:1901594/133‑1 (MQ=255)
                                                                                                          gAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAAT‑ACAAGCAGTTAATTGCGCTAATACATATTGGCAAc                                       <  1:2384502/150‑1 (MQ=255)
                                                                                                               gcAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGc                                                                                           >  1:1994276/1‑94 (MQ=255)
                                                                                                               gcAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGc                                                                                           <  2:1994276/94‑1 (MQ=255)
                                                                                                                   gAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCAt                                                                                              >  2:927032/1‑87 (MQ=255)
                                                                                                                   gAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCAt                                                                                              <  1:927032/87‑1 (MQ=255)
                                                                                                                   gAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCA‑ATAATTGCGCTAATACATATTGGCAACTGATGtttt                              >  1:150162/1‑150 (MQ=255)
                                                                                                                      cTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCaa                                                                                >  1:3008494/1‑98 (MQ=255)
                                                                                                                      cTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCaa                                                                                <  2:3008494/98‑1 (MQ=255)
                                                                                                                       tGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCA‑ATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTg                          <  1:1177496/150‑1 (MQ=255)
                                                                                                                       tGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCA‑ATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTg                          >  1:2358461/1‑150 (MQ=255)
                                                                                                                         gATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCA‑ATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCt                        >  2:3044217/1‑150 (MQ=255)
                                                                                                                               tCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCA‑ATAATTGCGCTAATACATATTGGCAACTGATGttttt                             >  2:290/1‑139 (MQ=255)
                                                                                                                               tCGGCGTTTTGCCCAACCGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCA‑ATAATTGCGCTAATACATATTGGCAACTGATGttttt                             <  1:290/139‑1 (MQ=255)
                                                                                                                                cGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACaa                                                                                     <  2:3038533/83‑1 (MQ=255)
                                                                                                                                cGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACaa                                                                                     >  1:3038533/1‑83 (MQ=255)
                                                                                                                                 ggCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCAACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCA‑ATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCa                >  1:1178192/1‑150 (MQ=255)
                                                                                                                                 ggCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGCTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTc                                                                                         <  1:247234/78‑1 (MQ=255)
                                                                                                                                   cGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCA‑ATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGt              <  2:126059/150‑1 (MQ=255)
                                                                                                                                      tctGCCCACCCGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCAt                                                                                              <  2:2417667/66‑1 (MQ=255)
                                                                                                                                         gCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCA‑ATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGTTCCCGc        <  2:1178192/150‑1 (MQ=255)
                                                                                                                                             acagggTGGGCAGATGGCGCTGTTCTGACATTAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCA‑ATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGTTCCCGCCttt    >  2:1725426/4‑150 (MQ=255)
                                                                                                                                              ccGGGTGGGCAGAGGGCGCTGTTCTGACATGAACCCTCCCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCA‑ATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCGATTATCCAGTTCCCGCCtttt   <  2:242912/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
GTATGACAGCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCA‑ATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGTTCCCGCCTTTTT  >  NC_002947/104732‑105024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑