BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,310,130	A→T	6.7%	L470Q (CTG→CAG)	PP_1144 ←	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,310,130	0	A	T	6.7%	307.6 / 11.3	104	L470Q (CTG→CAG)	PP_1144	membrane protein
Reads supporting (aligned to +/- strand): ref base A (42/55); new base T (4/3); total (46/58)
Fisher's exact test for biased strand distribution p-value = 6.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.58e-01

Only 100 of 105 total aligned reads displayed.

GGCAATGCCGACAAAACGGTCGGGCCCGAGCAGGCCGTGTTGAGGATGCTGCCAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCAGGTCGGCATGCTTGAGCAGCTCTTCGGCTGTG  >  NC_002947/1309982‑1310270

ggCAATGCCGACAAAACGGTCGGGCCCGAGCAGGCAGTGTTGAGGATGCTGCCAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCATACACAGAGCGCGCTCTTAGAGCAGCGCCac                                                                                                                                             >  2:154959/1‑149 (MQ=255)

  cAATGCCGACAAAACGGTCGGGCCCGAGCAGGCCGTGTTGAGGATGCTGCCAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGgc                                                                                                                                           <  2:2621718/150‑1 (MQ=255)

    aTGCCGACTAAACGGTCGGGCCAGAGCAGGCCGTGTTGAGGATGCTGCCAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGgcgc                                                                                                                                         <  1:779397/150‑1 (MQ=255)

       ccGACAAAACGGTCGGGCCCGAGCAGGCCGTGTTGAGGATGCTGCCAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGc                                                                                                                                      <  2:2655364/150‑1 (MQ=255)

        cGACAAAACGGTCGGGCCCGAGCAGGCCGTGTTGAGGATGCTGCCAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGct                                                                                                                                     >  2:307187/1‑150 (MQ=255)

            aaaaCGGTCAGGACTGCGCCGGCCGTGTTGAGGATGCTGCCAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCtcttc                                                                                                                                 <  2:710348/150‑1 (MQ=255)

               aCGGTCGGGCCCGAGCAGGCCGTGTTGAGGATGCTGCCAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCttcttc                                                                                                                              <  2:465859/150‑1 (MQ=255)

               aCGGTCGGGCCCGAGCAGGCCGTGTTGAGGATGCTGCCAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCttcttc                                                                                                                              <  1:1792093/150‑1 (MQ=255)

               aCGGTCGGGCCCGAGCAGGCCGTGTTGAGGATGCTGCCAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCttcttc                                                                                                                              <  1:1293455/150‑1 (MQ=255)

                 ggTCGGGCCCGAGCAGGCCGTGTTGAGGATGCTGCCAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCa                                                                                                                            <  2:1317459/150‑1 (MQ=255)

                    cGGGCCCGAGCAGGCCGTGTTGAGGATGCTGCCAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGtt                                                                                                                         <  1:2510650/150‑1 (MQ=255)

                      ggCCCGAGCAGGCCGTGTTGAGGATGCTGCCAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGc                                                                                                                       >  2:2314476/1‑150 (MQ=255)

                         ccGAGCAGGCCGTGTTGAGGATGCTGCCAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAg                                                                                                                    <  2:1670852/150‑1 (MQ=255)

                         ccGAGCAGGCCGTGTTGAGGATGCTGCCAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGCAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAg                                                                                                                    <  1:2224659/150‑1 (MQ=255)

                          cGAGCAGGCCGTGTTGAGGATGCTGCCAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAgg                                                                                                                   <  2:778861/150‑1 (MQ=255)

                                 gCCGTGTTGAGGATGCTGCCAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGa                                                                                                            >  1:723772/1‑150 (MQ=255)

                                 gCCGTGTTGAGGATGCTGCCAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGa                                                                                                            >  1:1226337/1‑150 (MQ=255)

                                  ccGTGTTGAGGATGCTGCCAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGAc                                                                                                           <  2:1220064/150‑1 (MQ=255)

                                  ccGTGTTGAGGATGCTGCCAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGAc                                                                                                           <  2:2939100/150‑1 (MQ=255)

                                  ccGTGTTGAGGATGCTGCCAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGAc                                                                                                           <  1:3072072/150‑1 (MQ=255)

                                   cGTGTTGAGGATGCTGCCAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGAcg                                                                                                          >  2:354262/1‑150 (MQ=255)

                                    gtgtTGAGGCTGCTCCCTGCGGACCAGCGCTGCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGAcgc                                                                                                         <  2:79083/150‑1 (MQ=255)

                                     tgtTGAGGATGCTGCCAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCttcttc                                                                                                                              >  1:1844992/1‑128 (MQ=255)

                                     tgtTGAGGATGCTGCCAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCttcttc                                                                                                                              <  2:1844992/128‑1 (MQ=255)

                                       ttGAGGATGCGGCCAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGATCGAAGATCGGCTGGTAGTGCACACACAGCACGCTCTCTTCGAGCAGGCACAGGCGCAGCTCTCCTTCCAGTTGCAATGCGATCGAcgcgcg                                                                                                      >  2:1292684/1‑150 (MQ=255)

                                         gAGGATGCTGCCAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAg                                                                                                    >  2:2874133/1‑150 (MQ=255)

                                                     aGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGt                                                                                        >  2:1092456/1‑150 (MQ=255)

                                                       cGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCa                                                                                      <  1:307187/150‑1 (MQ=255)

                                                        gTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCAt                                                                                     <  1:2395811/150‑1 (MQ=255)

                                                        gTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCAt                                                                                     <  2:2583656/150‑1 (MQ=255)

                                                         tACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCAtt                                                                                    <  1:1810910/150‑1 (MQ=255)

                                                         tACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCAtt                                                                                    <  2:723772/150‑1 (MQ=255)

                                                          aCCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCTCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTg                                                                                   >  1:1504175/1‑150 (MQ=255)

                                                           ccAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGAt                                                                                                               <  1:964014/121‑1 (MQ=255)

                                                            cAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTgaa                                                                                 >  2:3025007/1‑150 (MQ=255)

                                                             aGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGAACACACAGCCCGCGCTGTTCGGGCGATGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTgaag                                                                                >  1:1145245/1‑150 (MQ=255)

                                                              gcgcTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTgaaga                                                                               >  1:915938/1‑150 (MQ=255)

                                                                gcTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGaaa                                                                             <  2:311229/150‑1 (MQ=255)

                                                                  ttCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCtctt                                                                                                                                  >  2:2783490/1‑95 (MQ=255)

                                                                  ttCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCtctt                                                                                                                                  <  1:2783490/95‑1 (MQ=255)

                                                                  ttCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGaaa                                                                             <  1:3023609/148‑1 (MQ=255)

                                                                  ttCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTAGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGaaa                                                                             >  2:3023609/1‑148 (MQ=255)

                                                                   tCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGAAACTc                                                                          <  1:3059776/150‑1 (MQ=255)

                                                                    ccAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGAAATTCa                                                                         >  1:1499332/1‑150 (MQ=255)

                                                                     cAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGAAATTCAg                                                                        <  1:2874133/150‑1 (MQ=255)

                                                                      aGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGAAATTCAgg                                                                       <  1:354262/150‑1 (MQ=255)

                                                                      aGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGAAATTCAgg                                                                       >  2:2508425/1‑150 (MQ=255)

                                                                      aGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGAAATTCAgg                                                                       <  1:2265552/150‑1 (MQ=255)

                                                                            gCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGtt                                                                                                                         >  1:841735/1‑94 (MQ=255)

                                                                            gCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGtt                                                                                                                         <  2:841735/94‑1 (MQ=255)

                                                                            gCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGAAATTCAGGATGTTg                                                                 >  1:2582767/1‑150 (MQ=255)

                                                                              cACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGt                                                                                        <  2:1091328/125‑1 (MQ=255)

                                                                              cACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGt                                                                                        >  1:1091328/1‑125 (MQ=255)

                                                                                    cccGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGtt                                                         <  1:771823/150‑1 (MQ=255)

                                                                                     ccGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTg                                                        <  2:2361883/150‑1 (MQ=255)

                                                                                      cGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGt                                                       >  2:457288/1‑150 (MQ=255)

                                                                                       gCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGAGCGAGCATTCCGGGTGTCATTTAAGAATTTCAGTCTGTTGCGCCCGTTGTc                                                      >  2:2639381/1‑150 (MQ=255)

                                                                                           cgcAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTg                                                  >  1:1444582/1‑150 (MQ=255)

                                                                                                gTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTg                                             >  2:2238799/1‑150 (MQ=255)

                                                                                                 tCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTgg                                            >  2:3030837/1‑150 (MQ=255)

                                                                                                 tCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTgg                                            >  1:1710519/1‑150 (MQ=255)

                                                                                                      gATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTagag                                       <  1:2639381/150‑1 (MQ=255)

                                                                                                       aTCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAgg                                      <  1:2016414/150‑1 (MQ=255)

                                                                                                        tCGGCTGGTAGTGCCCACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTTTTCTTCCAGTTTCAAGGCGAGCGACGCGCGAGCCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAggg                                     <  2:818246/150‑1 (MQ=255)

                                                                                                           gCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTCCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCa                                  >  2:1294134/1‑150 (MQ=255)

                                                                                                               gTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGAAATTCAgg                                                                       <  1:157645/109‑1 (MQ=255)

                                                                                                               gTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGAAATTCAgg                                                                       >  2:157645/1‑109 (MQ=255)

                                                                                                                 aGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCAGGTCgg                            <  1:457288/150‑1 (MQ=255)

                                                                                                                     cacacacaGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCAGGTCGGCATg                        >  1:2447903/1‑150 (MQ=255)

                                                                                                                      acacacaGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCAGGTCGGCATGc                       <  1:2314476/150‑1 (MQ=255)

                                                                                                                        acacaGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTCCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCAGGTCGGCATGCtt                     <  1:1294134/150‑1 (MQ=255)

                                                                                                                          acaGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGAAATTCAGGCTGtt                                                                  <  2:2963791/103‑1 (MQ=255)

                                                                                                                          acaGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGAAATTCAGGCTGtt                                                                  >  1:2963791/1‑103 (MQ=255)

                                                                                                                          acaGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCAGGTCGGCATGCTTGa                   <  2:915938/150‑1 (MQ=255)

                                                                                                                          acaGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCAGGTCGGCATGCTTGa                   >  1:239898/1‑150 (MQ=255)

                                                                                                                            aGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCAGGTCGGCATGCTTGagc                 >  1:611053/1‑150 (MQ=255)

                                                                                                                            aGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCAGGTCGGCATGCTTGagc                 <  1:154959/150‑1 (MQ=255)

                                                                                                                            aGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCAGGTCGGCATGCTTGagc                 >  1:1459954/1‑150 (MQ=255)

                                                                                                                              cccGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCAGGTCGGCATGCTTGagcag               >  1:1316223/1‑150 (MQ=255)

                                                                                                                               ccGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGtt                                                         >  1:1095265/1‑107 (MQ=255)

                                                                                                                               ccGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGtt                                                         <  2:1095265/107‑1 (MQ=255)

                                                                                                                                cgcgCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCAGGTCGGCATGCTTGAGCAGct             >  1:156405/1‑150 (MQ=255)

                                                                                                                                   gcTCTTCGAGCAGCGCCATTCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGAAATTCAGGCTGtt                                                                  <  2:2549367/94‑1 (MQ=255)

                                                                                                                                   gcTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGAAATTCAGGCTGtt                                                                  >  1:2549367/1‑94 (MQ=255)

                                                                                                                                     aagaagAGCTGCGCCTGGCGCTGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTg                                                  >  1:292563/6‑108 (MQ=255)

                                                                                                                                      ctTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGACCTTGGATTGGTAGAGGGCCAGGTCGGCATGCTTGAGCAGCTCTTCgg       <  2:315010/150‑1 (MQ=255)

                                                                                                                                       ttCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGt                                                                                        >  2:1672938/1‑68 (MQ=255)

                                                                                                                                       ttCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGt                                                                                        <  1:1672938/68‑1 (MQ=255)

                                                                                                                                       ttCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCAGGTCGGCATGCTTGAGCAGCTCTTCGGc      <  2:1710519/150‑1 (MQ=255)

                                                                                                                                       gaagAGCTGCGCCTGGCGCTGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCtt                                                   <  1:1559643/102‑1 (MQ=255)

                                                                                                                                       gaagAGCTGCGCCTGGCGCTGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCtt                                                   >  2:1559643/4‑105 (MQ=255)

                                                                                                                                         agAGCTGCGCCTGGCGCTGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCtt                                                   <  1:293945/102‑1 (MQ=255)

                                                                                                                                         agAGCTGCGCCTGGCGCTGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCtt                                                   >  2:293945/2‑103 (MQ=255)

                                                                                                                                          gAGCTGCGCCTGGCGCTGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGAGGCTCTTGAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCAGGTCGGCATGCTTGAGCAGCTCTTCGGCtgt   >  1:219254/1‑150 (MQ=255)

                                                                                                                                          gAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGATGCGCGAGCCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCtt                                                   >  2:2026678/1‑102 (MQ=255)

                                                                                                                                          gAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGATGCGCGAGCCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCtt                                                   <  1:2026678/102‑1 (MQ=255)

                                                                                                                                          gAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCAGGTCGGCATGCt                      <  2:817005/131‑1 (MQ=255)

                                                                                                                                          gAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCAGGTCGGCATGCt                      >  1:817005/1‑131 (MQ=255)

                                                                                                                                          gAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCAGGTCGGCATGCTTGAGCAGCTCTTCGGCtgt   >  2:175133/1‑150 (MQ=255)

                                                                                                                                           agcagcGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCAGGTCGGCATGCTTGAGCAGCTCTTCGGCtgtg  <  1:2508425/150‑1 (MQ=255)

GGCAATGCCGACAAAACGGTCGGGCCCGAGCAGGCCGTGTTGAGGATGCTGCCAGCGTACCAGCGCTTCCAGTTTGGCCACTTGCCCGCTGCGCAGGTCGAAGATCGGCTGGTAGTGCACACACAGCCCGCGCTCTTCGAGCAGCGCCAGGCGCAGCTCTTCTTCCAGTTGCAAGGCGATCGACGCGCGAGCCTTCAGGCTGTCATTGAAGAAATTCAGGCTGTTGCGCCCGTTGTCCTTGGATTGGTAGAGGGCCAGGTCGGCATGCTTGAGCAGCTCTTCGGCTGTG  >  NC_002947/1309982‑1310270

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`