Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,356,411 G→C 12.0% G94A (GGC→GCC)  PP_1181 → two‑component system response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,356,4110GC12.0% 383.5 / 41.2 134G94A (GGC→GCC) PP_1181two‑component system response regulator
Reads supporting (aligned to +/- strand):  ref base G (66/52);  new base C (8/8);  total (74/60)
Fisher's exact test for biased strand distribution p-value = 7.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.67e-01

Only 100 of 137 total aligned reads displayed.

ATCATCGACAGCCAGCCCGACCTGGTGATCCTCGACCTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCTTCGGTGCGCTGCGTATCGACAACCGCC  >  NC_002947/1356263‑1356560
                                                                                                                                                    |                                                                                                                                                     
atcatcGACAGCCAGCCCGACCTGGTGATCCTCGACCTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGc                                                                                                                                                      <  1:84293/150‑1 (MQ=255)
  catcGACAGCCAGCCCGACCTGGTGATCCTCGACCTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCt                                                                                                                                                    <  2:1731156/150‑1 (MQ=255)
  catcGACAGCCAGCCCGACCTGGTGATCCTCGACCTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCt                                                                                                                                                    <  1:872716/150‑1 (MQ=255)
      gACAGCCAGCCCGACCTGGTGATCCTCGACCTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGCCCTGGAc                                                                                                                                                >  1:1168629/1‑150 (MQ=255)
         agccagccCGACCTGGTGATCCTCGACCTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGCCCTGGACCTg                                                                                                                                             <  2:1168629/150‑1 (MQ=255)
           ccagccCGACCTGGTGATCCTCGACCTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTggg                                                                                                                                           >  2:1717963/1‑150 (MQ=255)
            cagccCGACCTGGTGATCCTCGACCTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCt                                                                                                                                              >  1:1375750/1‑146 (MQ=255)
              gccCGACCTGGTGATCCTCGACCTGATGCTCCCCGGCGAGGACGGCCTGATCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATACTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGgcgc                                                                                                                                        <  1:387180/150‑1 (MQ=255)
                  gACCTGGTGATCCTCGACCTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCcgac                                                                                                                                    >  1:881632/1‑150 (MQ=255)
                     cTGGTGATCCTCGACCTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTg                                                                                                                                                   <  1:561820/132‑1 (MQ=255)
                        gTGATCCTCGACCTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTgg                                                                                                                                            <  2:2768748/136‑1 (MQ=255)
                        gTGATCCTCGACCTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTAc                                                                                                                              >  1:2353803/1‑150 (MQ=255)
                           aTCCTCGACCTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTc                                                                                                                           <  2:711330/150‑1 (MQ=255)
                            tCCTCGACCTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTTATGAACTGGACCAGGTCCAGGCCCTGGACCTgg                                                                                                                                            <  1:2343523/132‑1 (MQ=255)
                            tCCTCGACCTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTTATGAACTGGACCAGGTCCAGGCCCTGGACCTgg                                                                                                                                            >  2:2343523/1‑132 (MQ=255)
                              cTCGACCTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGc                                                                                                                        >  1:2071357/1‑150 (MQ=255)
                                  aCCTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAAc                                                                                                                    <  1:365326/150‑1 (MQ=255)
                                   ccTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAAcc                                                                                                                   >  2:43242/1‑150 (MQ=255)
                                   ccTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAAcc                                                                                                                   >  2:1031851/1‑150 (MQ=255)
                                     tGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCg                                                                                                                 >  1:505409/1‑150 (MQ=255)
                                      gATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGt                                                                                                                <  2:881632/150‑1 (MQ=255)
                                      gATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCGCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGATCTGGGCGCCGACGACTACGTCTGCAAACCCGt                                                                                                                >  2:502133/1‑150 (MQ=255)
                                           tCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGcc                                                                                                           <  1:2451591/150‑1 (MQ=255)
                                           tCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGcc                                                                                                           <  1:1248289/150‑1 (MQ=255)
                                           tCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGCCCTGGACCTg                                                                                                                                             <  1:1846362/116‑1 (MQ=255)
                                           tCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGCCCTGGACCTg                                                                                                                                             >  2:1846362/1‑116 (MQ=255)
                                            ccccGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGAc                                                                                                                                                >  1:2329837/1‑112 (MQ=255)
                                            ccccGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGAc                                                                                                                                                <  2:2329837/112‑1 (MQ=255)
                                             cccGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCa                                                                                                         <  1:328513/150‑1 (MQ=255)
                                             cccGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCa                                                                                                         >  1:1173779/1‑150 (MQ=255)
                                             cccGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCa                                                                                                         >  2:518587/1‑150 (MQ=255)
                                                  cGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCtt                                                                                                    <  2:2139529/150‑1 (MQ=255)
                                                   gAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCtt                                                                                                    >  2:359952/1‑149 (MQ=255)
                                                      gACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCAACGCTtgctg                                                                                                >  1:1346897/1‑150 (MQ=255)
                                                       aCGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTtgctgc                                                                                               >  1:3037802/1‑150 (MQ=255)
                                                          gCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTgg                                                                                            <  2:1984435/150‑1 (MQ=255)
                                                             tGAGCATCTGCCTCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCAc                                                                                         <  2:1541317/150‑1 (MQ=255)
                                                             tGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCAc                                                                                         >  1:355440/1‑150 (MQ=255)
                                                             tGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGCGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCAc                                                                                         <  2:1038547/150‑1 (MQ=255)
                                                                  aTCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATc                                                                                    >  1:1532476/1‑150 (MQ=255)
                                                                            gggTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCctgc                                                                          <  1:1084611/150‑1 (MQ=255)
                                                                              gTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTgcgc                                                                                                            >  1:1559070/1‑114 (MQ=255)
                                                                              gTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTgcgc                                                                                                            <  2:1559070/114‑1 (MQ=255)
                                                                                gcgcAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTgcg                                                                      <  2:1532476/150‑1 (MQ=255)
                                                                                  gcAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTgg                                                                                                                                            >  1:1639241/1‑78 (MQ=255)
                                                                                  gcAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTgg                                                                                                                                            <  2:1639241/78‑1 (MQ=255)
                                                                                  gcAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGcgcc                                                                    >  1:2184684/1‑150 (MQ=255)
                                                                                      ccAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGCCCTGGACCTg                                                                                                                                             >  2:882845/1‑73 (MQ=255)
                                                                                      ccAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGCCCTGGACCTg                                                                                                                                             <  1:882845/73‑1 (MQ=255)
                                                                                            ccAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAAc                                                          >  2:2009880/1‑150 (MQ=255)
                                                                                             cAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAAcc                                                         >  1:673434/1‑150 (MQ=255)
                                                                                              aGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCg                                                        >  1:280878/1‑150 (MQ=255)
                                                                                                    cGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACa                                                  <  1:1939130/150‑1 (MQ=255)
                                                                                                    cGATCCTCATGCTCACCGCGCGCAGTGATGAAATGGAACAGCTACAGGGTCTGGACCGGTGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTCTCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACa                                                  <  2:673434/150‑1 (MQ=255)
                                                                                                     gATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAg                                                 >  2:2730495/1‑150 (MQ=255)
                                                                                                      aTCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGc                                                >  1:2501289/1‑150 (MQ=255)
                                                                                                          tCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAgc                                            >  1:2472386/1‑150 (MQ=255)
                                                                                                          tCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAgc                                            >  2:1984256/1‑150 (MQ=255)
                                                                                                            aTGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCt                                                                                                                          <  1:1669409/70‑1 (MQ=255)
                                                                                                            aTGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCt                                                                                                                          >  2:1669409/1‑70 (MQ=255)
                                                                                                              gCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCa                                        <  2:558603/150‑1 (MQ=255)
                                                                                                               atCACTGCGCGCGGTGATGAACTGGACCAGGTCCAGGGCCTGGAc                                                                                                                                                <  2:1914587/44‑1 (MQ=25)
                                                                                                               atCACTGCGCGCGGTGATGAACTGGACCAGGTCCAGGGCCTGGAc                                                                                                                                                >  1:1914587/2‑45 (MQ=25)
                                                                                                                 cACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGa                                     <  1:1031851/150‑1 (MQ=255)
                                                                                                                  cccGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGAc                                    <  1:1460017/149‑1 (MQ=255)
                                                                                                                   ccGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGt                                                       <  1:12210/130‑1 (MQ=255)
                                                                                                                   ccGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGt                                                       >  2:12210/1‑130 (MQ=255)
                                                                                                                    cgcgcgCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCt                                  <  2:1817471/150‑1 (MQ=255)
                                                                                                                     gcgcgcAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTg                                 <  2:401580/150‑1 (MQ=255)
                                                                                                                      cgcgcAGTGATGAACTGGACCAGGTCCAGGGCCGGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTgg                                <  2:158840/150‑1 (MQ=255)
                                                                                                                       gcgcGGTGATGAACTGGACCAGGTCCAGGGCATGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAAGCGTGGACAGCAAGCGCCAGGACCTGGc                               >  1:111381/1‑150 (MQ=255)
                                                                                                                         gcAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCGGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCt                             >  2:2224823/1‑150 (MQ=255)
                                                                                                                            gTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCTTCg                          <  2:355440/150‑1 (MQ=255)
                                                                                                                            gTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCTTCg                          <  2:2472386/150‑1 (MQ=255)
                                                                                                                               atgaACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCtt                            >  1:2206467/1‑145 (MQ=255)
                                                                                                                                  aaCTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCTTCGGTgcgc                    <  2:2353803/150‑1 (MQ=255)
                                                                                                                                   aCTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTg                                 >  1:1141209/1‑136 (MQ=255)
                                                                                                                                    cTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCTTCGGTGCGCTg                  >  2:100609/1‑150 (MQ=255)
                                                                                                                                     tGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCa                                               <  1:2761270/120‑1 (MQ=255)
                                                                                                                                     tGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCa                                               >  2:2761270/1‑120 (MQ=255)
                                                                                                                                     tGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCTTCGGTGCGCTGc                 >  1:2507390/1‑150 (MQ=255)
                                                                                                                                       gACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACATGGCCTTCGGTGCGCTGCGt               >  1:1232537/1‑150 (MQ=255)
                                                                                                                                        aCCAGGTCCAGTGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCTTCGGTGCGCTGCGTa              >  2:2049596/1‑150 (MQ=255)
                                                                                                                                         ccAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCTTCGGTGCGCTGCGTAt             <  1:667298/150‑1 (MQ=255)
                                                                                                                                         ccAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCTTCGGTGCGCTGCGTAt             >  2:667298/1‑150 (MQ=255)
                                                                                                                                         ccAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCTTCGGTGCGCTGCGTAt             >  1:1662683/1‑150 (MQ=255)
                                                                                                                                         ccAGGTCCAGGCCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCa                                                                                  <  2:2593070/81‑1 (MQ=255)
                                                                                                                                         ccAGGTCCAGGCCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCa                                                                                  >  1:2593070/1‑81 (MQ=255)
                                                                                                                                         ccAGGTCCAGGCCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGAc                                    <  1:2042769/127‑1 (MQ=255)
                                                                                                                                         ccAGGTCCAGGCCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCTTCGGTGCGCTGc                 >  2:2415380/1‑146 (MQ=255)
                                                                                                                                         ccAGGTCCAGGCCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCTTCGGTGCGCTGc                 <  1:2415380/146‑1 (MQ=255)
                                                                                                                                          cAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCTTCGGTGCGCTGCGTATc            >  1:2545110/1‑150 (MQ=255)
                                                                                                                                          cAGGTCCAGGCCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGaaa                                                           >  2:2265762/1‑103 (MQ=255)
                                                                                                                                          cAGGTCCAGGCCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGaaa                                                           <  1:2265762/103‑1 (MQ=255)
                                                                                                                                           aGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCTTCGGTGCGCTGCGTATCg           >  2:2302295/1‑150 (MQ=255)
                                                                                                                                            ggTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTtgctgc                                                                                               >  2:375422/1‑65 (MQ=255)
                                                                                                                                            ggTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTtgctgc                                                                                               <  1:375422/65‑1 (MQ=255)
                                                                                                                                             gTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACTTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCTTCGGTGCGCTGCGTATCGAc         >  2:2400609/1‑150 (MQ=255)
                                                                                                                                                  gggCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCTTCGGTGCGCTGCGTATCGACAACCg    >  1:355496/1‑150 (MQ=255)
                                                                                                                                                    gCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCTTCGGTGCGCTGCGTATCGACAACCGcc  >  2:2538568/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ATCATCGACAGCCAGCCCGACCTGGTGATCCTCGACCTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCTTCGGTGCGCTGCGTATCGACAACCGCC  >  NC_002947/1356263‑1356560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑