Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,403,597	T→C	33.8%	intergenic (‑57/+24)	PP_1227 ← / ← PP_1228	membrane protein/methyl‑accepting chemotaxis transducer

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,403,597	0	T	C	33.8%	41.9 / 35.1	45	intergenic (‑57/+24)	PP_1227/PP_1228	membrane protein/methyl‑accepting chemotaxis transducer
Reads supporting (aligned to +/- strand):  ref base T (14/15);  new base C (6/9);  total (21/24)
Fisher's exact test for biased strand distribution p-value = 7.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.61e-01

TGGCGATGAACAGGGTGGCGGCGATCGAGGTGCGTAAAAGCCCTTGTTCGCTGGTGATGTCGAAGAAAGTGGATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATGGTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGTGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGTCTCGAATGCCGGCC  >  NC_002947/1403450‑1403744                                                                                                                                                    |                                                                                                                                                    tggCGATGAACAGGGTGGCGGCGATCGAGGTGCGTAAAAGCCCTTGTTCGCTGGTGATGTCGAAGAAAGTGGATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATGGTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGTGc                                                                                                                                                   >  2:1335658/1‑150 (MQ=255)    cGATGAACAGGGTGGCGGCGATCGAGGTGCGTAAAAGCCCTTGTTCGCTGGTGATGTCGAAGAAAGTGGATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATGGTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGTGCCcg                                                                                                                                                <  2:2115646/150‑1 (MQ=255)        gAACAGGGTGGCGGCGATCGAGGTGCGTAAAAGCCCTTGTTCGCTGGTGATGTCGAAGAAAGTGGATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATACCGATGGTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGTGCCCGCGaa                                                                                                                                            <  2:440637/150‑1 (MQ=255)        gAACAGGGTGGCGGCGATCGAGGTGCGTAAAAGCCCTTGTTCGCTGGTGATGTCGAAGAAAGTGGATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATGGTGCGGTGCCTGTCAGGGCCTCTTCGCTGGCACGCCCGCGaa                                                                                                                                            <  2:2862211/150‑1 (MQ=255)        gAACAGGGTGGCGGCGATCGAGGTGCGTAAAAGCCCTTGTTCGCTGGTGATGTCGAAGAAAGTGGATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATGGTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGTGccctctaa                                                                                                                                            <  2:115300/150‑6 (MQ=255)        gAACAGGGTGGCGGCGATCGAGGTGCGTAAAAGCCCTTGTTCGCTGGTGATGTCGAAGAAAGTGGATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATGGTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGTGCCCGCGaa                                                                                                                                            <  2:3071209/150‑1 (MQ=255)          aCAGGGTGGCGGCGATCGAGGTGCGTAAAAGCCCTTGTTCGCTGGTGATGTCGAAGAAAGTGGATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATGGTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGTGCCCGCGAaga                                                                                                                                          <  1:1335658/150‑1 (MQ=255)          aCAGGGTGGCGGCGATCGAGGTGCGTAAAAGCCCTTGTTCGCTGGTGATGTCGAAGAAAGTGGATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATGGTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCACGCCCGCGAaga                                                                                                                                          <  2:1372863/150‑1 (MQ=255)          aCAGGGTGGCGGCGATCGAGGTGCGTAAAAGCCCTTGTTCGCTGGTGATGTCGAAGAAAGTGGACCGGAAGGGATTAGGCATAAGTTGTGCTCAAGAATAACGATGGTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCACGCCCGCGAaga                                                                                                                                          <  1:191709/150‑1 (MQ=39)          aCAGGGTGGCGGCGATCGAGGTGCGTAAAAGCCCTTGTTCGCTGGGGATGTCGAAGAAAGTGGATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATGGTGCGGTGCCAGTCAGGGCCTCTTCGCGGGCGTGCCCGCGAaga                                                                                                                                          <  1:720205/150‑1 (MQ=255)           cAGTGTGGCGGCTATCGAGGTGCGTAAAATCCCTTGTTCGCTGGTGATGTCGAAAAAAGTGGATCGGGTTGCATTAGGCATAAGTTGTGCTCCAGAATACCTATGGTCCGGTTCCTTTCAGGGCCTCTTCGCGGGCACGCCCGCGAagag                                                                                                                                         <  2:2652760/150‑1 (MQ=255)           cAGGGTGGCGGCGATCGAGGTGCGTAAAAGCCCTTGTTCGCTGGTGATGTCGAAGAAAGTGGATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATGGTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGTGCCCGCGAagag                                                                                                                                         <  1:1203762/150‑1 (MQ=255)              ggTGGCGGCGATCGAGGTGCGTAAAAGCCCTTGTTCGCTGGTGATGTCGAAGAAAGTGGATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATGGTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGTGCCCGCGAag                                                                                                                                           >  2:2206961/1‑145 (MQ=255)              ggTGGCGGCGATCGAGGTGCGTAAAAGCCCTTGTTCGCTGGTGATGTCGAAGAAAGTGGATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATGGTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGTGCCCGCGAag                                                                                                                                           <  1:2206961/145‑1 (MQ=255)                    ggcgATCGAGGTGCGTAAAAGCCCTTGTTCGCTGGTGATGTCGAAGAAAGTGGATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATGGTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGTGCCCGCGAagag                                                                                                                                         <  1:1194524/141‑1 (MQ=255)                    ggcgATCGAGGTGCGTAAAAGCCCTTGTTCGCTGGTGATGTCGAAGAAAGTGGATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATGGTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGTGCCCGCGAagag                                                                                                                                         >  2:1194524/1‑141 (MQ=255)                    ggcgATCGAGGTGCGTAAAAGCCCTTGTTCGCTGGTGATGTCGAAGAAAGTGGATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATGGTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGTGCCCGCGAAGAGGCCCTGACa                                                                                                                                <  2:1592819/150‑1 (MQ=255)                                   tAAAAGCCCTTGTTCGCTGGTGATGTCGAAGAAAGTGTATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATGGTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCACGCCCGCGa                                                                                                                                             >  2:2037030/1‑122 (MQ=255)                                   tAAAAGCCCTTGTTCGCTGGTGATGTCGAAGAAAGTGTATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATGGTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCACGCCCGCGa                                                                                                                                             <  1:2037030/122‑1 (MQ=255)                                                      gTGATGTCGAAGAAAGTGGATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATGGTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGTGCCCGCGAagag                                                                                                                                         <  2:2294983/107‑1 (MQ=255)                                                      gTGATGTCGAAGAAAGTGGATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATGGTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGTGCCCGCGAagag                                                                                                                                         >  1:2294983/1‑107 (MQ=255)                                                                                                       aaGAATAACGATGGTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCACGCCCGCGAaga                                                                                                                                          >  1:1880551/1‑57 (MQ=18)                                                                                                       aaGAATAACGATGGTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCACGCCCGCGAaga                                                                                                                                          <  2:1880551/57‑1 (MQ=17)                                                                                                                 aTGGTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGTGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGCCTGCcca                                   >  2:1489455/1‑150 (MQ=255)                                                                                                                              tGTCAGGGCCTCTTCGCGGGCGTGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGa                                                   >  2:2418736/1‑121 (MQ=255)                                                                                                                              tGTCAGGGCCTCTTCGCGGGCGTGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGa                                                   <  1:2418736/121‑1 (MQ=255)                                                                                                                                     gCCGCTTCTCGTGGGGGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGtc               >  2:2817691/1‑150 (MQ=255)                                                                                                                                       ctctTCGCGGGCGTGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCt                                                                    >  2:2318516/1‑95 (MQ=255)                                                                                                                                       ctctTCGCGGGCGTGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCt                                                                    <  1:2318516/95‑1 (MQ=255)                                                                                                                                       ctctTCGCGGGCACGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGtctc             >  2:1980266/1‑150 (MQ=255)                                                                                                                                        tctTCGCGGGCGTGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGTCTCg            >  2:864453/1‑150 (MQ=255)                                                                                                                                        tctTCGCGGGCGTGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGTCTCg            >  2:1825369/1‑150 (MQ=255)                                                                                                                                        tctTCGCGGGCGTGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCACCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCAcacggcc            >  1:1992272/1‑144 (MQ=255)                                                                                                                                        tctTCGCGGGCACGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGcc                                                                                                  >  1:2677900/1‑64 (MQ=21)                                                                                                                                        tctTCGCGGGCACGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGcc                                                                                                  <  2:2677900/64‑1 (MQ=21)                                                                                                                                        tctTCGCGGGCACGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGCCTGCccagc                                 >  2:2511146/1‑129 (MQ=255)                                                                                                                                        tctTCGCGGGCACGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGCCTGCccagc                                 <  1:2511146/129‑1 (MQ=255)                                                                                                                                         ctTCGCGGGCGTGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGTCTCGa           >  1:802872/1‑150 (MQ=255)                                                                                                                                         ctTCGCGGGCACGCCCGCGAAGAGGCCCTGACAGGCTAGAGGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGc                                          >  1:2918085/1‑119 (MQ=255)                                                                                                                                         ctTCGCGGGCACGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGc                                          <  2:2918085/119‑1 (MQ=255)                                                                                                                                          ttCGCGGGCGTGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGTCTCGaa          >  2:1183754/1‑150 (MQ=255)                                                                                                                                             gcgGGCGTGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCtt                                                                   >  2:1046394/1‑90 (MQ=255)                                                                                                                                             gcgGGCGTGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCtt                                                                   <  1:1046394/90‑1 (MQ=255)                                                                                                                                               gggCGTGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGTCTCGAATGCCg     >  1:1621713/1‑150 (MQ=255)                                                                                                                                                  cGTGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGTCTCGAATGCCGGcc  <  1:1183754/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    TGGCGATGAACAGGGTGGCGGCGATCGAGGTGCGTAAAAGCCCTTGTTCGCTGGTGATGTCGAAGAAAGTGGATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATGGTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGTGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGTCTCGAATGCCGGCC  >  NC_002947/1403450‑1403744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑