Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,625,312 C→T 5.1% L523L (CTG→CTA)  nadB ← L‑aspartate oxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,625,3120CT 5.1% 239.8 / 2.3 81L523L (CTG→CTA) nadBL‑aspartate oxidase
Reads supporting (aligned to +/- strand):  ref base C (34/41);  new base T (2/2);  total (37/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.38e-01

GGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCTCGGCCACTTGCGCCAGGTTGCGCAGCTCGATCAGGTCACGGCTGACCTTGTAGTTGCTGTAGAACTCG  >  NC_002947/1625173‑1625456
                                                                                                                                           |                                                                                                                                                
ggTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTcc                                                                                                                                                  >  1:713720/1‑140 (MQ=255)
ggTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTcc                                                                                                                                                  <  2:713720/140‑1 (MQ=255)
        ttCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTg                                                                                                                                        <  2:741876/142‑1 (MQ=255)
        ttCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTg                                                                                                                                        >  1:741876/1‑142 (MQ=255)
        ttCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATcc                                                                                                                                >  2:1189694/1‑150 (MQ=255)
         tCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCa                                                                                                                               <  2:1753776/150‑1 (MQ=255)
         tCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCa                                                                                                                               <  1:1503526/150‑1 (MQ=255)
         tCAGCCGGCGGAGCTGGGCTGCGGTGAGCCGGGCATAGATCGAAACCGATTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACTACGCAGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCa                                                                                                                               <  1:1843760/150‑1 (MQ=255)
          cAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAg                                                                                                                              <  2:2067677/150‑1 (MQ=255)
           aGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAgt                                                                                                                             <  1:912009/150‑1 (MQ=255)
            gCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAgtg                                                                                                                            <  2:838458/150‑1 (MQ=255)
             ccGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAgtgt                                                                                                                           >  1:2867741/1‑150 (MQ=255)
                  ggcgCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAAt                                                                                                                      >  1:1083826/1‑150 (MQ=255)
                      cTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCaa                                                                                                                  <  2:2867741/150‑1 (MQ=255)
                       tGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCAACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAAc                                                                                                                 >  2:2261780/1‑150 (MQ=255)
                         cgCTGCGGCGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAAccc                                                                                                               >  2:2972100/2‑150 (MQ=255)
                           ttGCGGTGAGCCGGGCATAGATCGCGACCGGGTTCGCTTCTTCGGGGGCGGGCCCGCGCAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCCCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTc                                                                                                             <  2:2928080/149‑1 (MQ=255)
                                   aGCCGGGGATAGAGCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTcc                                                                                                     <  2:489268/150‑1 (MQ=255)
                                   aGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTcc                                                                                                     >  2:462799/1‑150 (MQ=255)
                                     ccGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCtt                                                                                                   >  1:270207/1‑150 (MQ=255)
                                         gCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGACAGGGATGGTGTGCTTGGCCTCATCGAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTgcgg                                                                                               >  1:206530/1‑149 (MQ=255)
                                          cATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCt                                                                                              <  2:1444160/150‑1 (MQ=255)
                                                tCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATg                                                                                                                     >  1:463155/1‑121 (MQ=255)
                                                tCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATg                                                                                                                     <  2:463155/121‑1 (MQ=255)
                                                tCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATgg                                                                                        >  1:696687/1‑150 (MQ=255)
                                                tCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATgg                                                                                        >  2:2964552/1‑150 (MQ=255)
                                                  gagaCCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCt                                                                                      <  2:2094982/150‑1 (MQ=255)
                                                  gagaCCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCt                                                                                      <  1:1189694/150‑1 (MQ=255)
                                                           ttAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGatc                                                                             <  1:2862224/150‑1 (MQ=255)
                                                            tAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGatca                                                                            <  1:462799/150‑1 (MQ=255)
                                                             aGCTTGTTCGCGGGAGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGatcat                                                                           <  2:605004/150‑1 (MQ=255)
                                                             aGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTgc                                                                                                 >  1:2119243/1‑128 (MQ=255)
                                                             aGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTgc                                                                                                 <  2:2119243/128‑1 (MQ=255)
                                                              gCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGatcatc                                                                          <  2:2142077/150‑1 (MQ=255)
                                                               cggcttcGCGGGCGAGCCCGCGAAGGCCACGACGACGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGAtcatca                                                                         <  2:1413058/147‑1 (MQ=255)
                                                                ttcttcGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTc                                                                                                      >  2:231372/1‑120 (MQ=255)
                                                                ttcttcGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTc                                                                                                      <  1:231372/120‑1 (MQ=255)
                                                                ttcttcGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAg                                                                        >  2:817488/1‑150 (MQ=255)
                                                                  cttcGCGGGCTCGCCCACGAGGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCTAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCt                                                                      >  1:1206221/1‑150 (MQ=255)
                                                                  cttcGCGGGCTCGCCCACGAGGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCTAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCt                                                                      >  1:1205938/1‑150 (MQ=255)
                                                                   ttcGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCTc                                                                     <  2:404402/150‑1 (MQ=255)
                                                                         ggCGAGCCCGCGAAGGCAACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCTCGGCCAc                                                               <  1:2261780/150‑1 (MQ=255)
                                                                          gCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCTCGGCCACt                                                              <  1:2325977/150‑1 (MQ=255)
                                                                            gAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCTCGGCCACTTg                                                            >  2:2680904/1‑150 (MQ=255)
                                                                             aGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCTCGGCCACTTgc                                                           <  2:2488821/150‑1 (MQ=255)
                                                                              gCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCTCGGCCACTTgcg                                                          >  2:683303/1‑150 (MQ=255)
                                                                                ccGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTATCCTTGCGCTGCATGGCTGACAGGATCATCAGCTCGGCCACTTgcgca                                                        >  1:1643942/1‑149 (MQ=255)
                                                                                     aaGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCTCGGCCACTTGCGCCAGGtt                                                   <  2:1083826/150‑1 (MQ=255)
                                                                                       ggCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCTCGGCCACTTGCGCCAGGTTgc                                                 <  1:2667745/150‑1 (MQ=255)
                                                                                       ggCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCTCGGCCACTTGCGCCAGGTTgc                                                 <  2:2514620/150‑1 (MQ=255)
                                                                                        gCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCTCGGCCACTTGCGCCAGGTTgcg                                                <  1:2964552/150‑1 (MQ=255)
                                                                                          cACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCTCGGCCACTTGCGCCAGGTTGCGCa                                              >  1:3106/1‑150 (MQ=255)
                                                                                          cACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAAGGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCTCGGCCACTTGCGCCAGGTTGCGCa                                              >  1:1400219/1‑150 (MQ=255)
                                                                                           acgacgCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGatcat                                                                           <  2:1964466/120‑1 (MQ=255)
                                                                                           acgacgCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGatcat                                                                           >  1:1964466/1‑120 (MQ=255)
                                                                                                gCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCTCGGCCACTTGCGCCAGGTTGCGCAGCTCGa                                        >  2:338193/1‑150 (MQ=255)
                                                                                                  cGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCTCGGCCACTTGCGCCAGGTTGCGCAGCTCGATc                                      >  1:2563351/1‑150 (MQ=255)
                                                                                                   gATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCTCGGCCACTTGCGCCAGGTTGCGCAGCTCGATCa                                     >  2:1644480/1‑150 (MQ=255)
                                                                                                    atatCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACCGGATCATCAGCTCGGCCACTTGCGCCAGGTTGCGCAGCTCGATCAg                                    <  1:714905/150‑1 (MQ=255)
                                                                                                     tatCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCTCGGCCACTTGCGCCAGGTTGCGCAGCTCGATCAg                                    >  2:288088/1‑149 (MQ=255)
                                                                                                     tatCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCTCGGCCACTTGCGCCAGGTTGCGCAGCTCGATCAg                                    <  1:288088/149‑1 (MQ=255)
                                                                                                      atCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGAtcatca                                                                         <  2:1136942/111‑1 (MQ=255)
                                                                                                      atCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGAtcatca                                                                         >  1:1136942/1‑111 (MQ=255)
                                                                                                      atCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCTCGGCCACTTGCGCCAGGTTGCGCAGCTCGATCAGGt                                  <  1:2680904/150‑1 (MQ=255)
                                                                                                           agCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCTCGGCCACTTGCGCCAGGTTGCGCAGCTCGATCAGGTCACgg                             >  1:1857236/1‑150 (MQ=255)
                                                                                                               ggTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCa                                                                                                                               >  2:1095411/1‑48 (MQ=255)
                                                                                                               ggTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCa                                                                                                                               <  1:1095411/48‑1 (MQ=255)
                                                                                                               ggTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCTCGGCCACTTGCGCCAGGTTGCGCAGCTCGATCAGGTCACGGCTGa                         <  2:206530/150‑1 (MQ=255)
                                                                                                                gTTAAGGATGGTGTCCTTGGCCTCGTCTAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCTCGGCCACTTGCGCCAGGTTGCGCAGCTCGATCAGGTCACGGCTGAc                        <  2:1206221/150‑1 (MQ=255)
                                                                                                                gTTAAGGATGGTGTCCTTGGCCTCGTCTAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCTCGGCCACTTGCGCCAGGTTGCGCAGCTCGATCAGGTCACGGCTGAc                        <  2:1205938/150‑1 (MQ=255)
                                                                                                                gTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCa                                                                                                                               <  2:2488578/47‑1 (MQ=255)
                                                                                                                gTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCa                                                                                                                               >  1:2488578/1‑47 (MQ=255)
                                                                                                                gTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGATGCATGGCTGACAGGATCATCAGCTCGGCCACTTGAGCCAGGTTGCGCAGCTCGATCAGGTCCCGGCTGAc                        >  1:1300127/1‑150 (MQ=255)
                                                                                                                    aGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAg                                                                                 <  1:21001/89‑1 (MQ=255)
                                                                                                                    aGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAg                                                                                 >  2:21001/1‑89 (MQ=255)
                                                                                                                    aGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCTCGGCCACTTGCGCCAGGTTGCGCAGCTCGATCAGGTCACGGCTGACCTTg                    <  2:271344/150‑1 (MQ=255)
                                                                                                                     ggATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCTCGGCCACTTGCGCCAGGTTGCGCAGCTCGATCAGGTCACGGCTGACCTTGt                   >  1:475918/1‑150 (MQ=255)
                                                                                                                      gATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCa                                                                                                                               <  1:2852427/41‑1 (MQ=255)
                                                                                                                      gATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCa                                                                                                                               >  2:2852427/1‑41 (MQ=255)
                                                                                                                            gttcTTGGCCTCGTCGAGGATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCTCGGCCACTTGCGCCAGGTTGCGCAGCTCGATCAGGTCACGGCTGACCTTGTAGTTGCt            <  2:1134504/147‑1 (MQ=255)
                                                                                                                               cTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCTCGGCCACTTGCGCCAGGTTGCGCAGCTCGATCAGGTCACGGCTGACCTTGTAGTTGCTGTa         >  2:1774854/1‑150 (MQ=255)
                                                                                                                               cTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCTCGGCCACTTGCGCCAGGTTGCGCAGCTCGATCAGGTCACGGCTGACCTTGTAGTTGCTGTa         <  2:3106/150‑1 (MQ=255)
                                                                                                                                      tCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTAAATGAGCATCAGGGCTGACAGGATCATCAGCTCGGCCACTTGCGCCAGGTTGCGCAGCTCGATCAGGTCACGGCTGACCTTGTAGTTGCTGTAGAACtcg  >  2:755363/1‑150 (MQ=255)
                                                                                                                                           |                                                                                                                                                
GGTTGAAGTTCAGCCGGCGGCGCTGGGCTGCGGTGAGCCGGGCATAGATCGAGACCGGGTTAGCTTCTTCGCGGGCGAGCCCGCGAAGGCCACGACGCCGATATCAGAGCGGGTTAAGGATGGTGTCCTTGGCCTCGTCCAGCATCCCTGGATAATCCAGTGTGTAATGCAACCCTCGGCTTTCCTTGCGCTGCATGGCTGACAGGATCATCAGCTCGGCCACTTGCGCCAGGTTGCGCAGCTCGATCAGGTCACGGCTGACCTTGTAGTTGCTGTAGAACTCG  >  NC_002947/1625173‑1625456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑