Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,667,961 C→T 7.2% A307A (GCC→GCT)  ffh → signal recognition particle GTPase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,667,9610CT 7.2% 392.5 / 15.6 138A307A (GCC→GCT) ffhsignal recognition particle GTPase subunit
Reads supporting (aligned to +/- strand):  ref base C (68/60);  new base T (6/4);  total (74/64)
Fisher's exact test for biased strand distribution p-value = 7.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.30e-01

Only 100 of 140 total aligned reads displayed.

CTGCGCTGTCGGTACGTGCAATCACCGGCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGCCTCGGCGGCCTGATGGACAAGCTGCCGAGCAT  >  NC_002947/1667813‑1668110
                                                                                                                                                    |                                                                                                                                                     
ctgcGCTGTCGGTACGTGCAATCACCGGCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCg                                                                                                                                                      >  2:1226048/1‑150 (MQ=255)
  gcgcTGTCGGTACGTGCAATCACCGGCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGCTCGATCAGGCTGAg                                                                                                                                                    <  2:76979/150‑1 (MQ=255)
  gcgcTGTCGGTACGTGCAATCACCGGCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGac                                                                                                                                                    <  2:2247797/150‑2 (MQ=255)
           gTACGTGCAATCTCCGTCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATc                                                                                                                                           <  2:490028/150‑1 (MQ=255)
           gTACGTGCAATCACCGGCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATc                                                                                                                                           <  2:2395417/150‑1 (MQ=255)
           gTACGTGCAATCACCGGCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATc                                                                                                                                           >  2:1763259/1‑150 (MQ=255)
           gTACGTGCAATCACCGGCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATc                                                                                                                                           <  1:804325/150‑1 (MQ=255)
           gTACGTGCAATCACCGGCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATc                                                                                                                                           <  1:1974879/150‑1 (MQ=255)
               gTGCAATCACCGGCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATc                                                                                                                                           <  2:2681081/146‑1 (MQ=255)
                tGCAATCACCGGCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGAcaa                                                                                                                                      >  2:2822795/1‑150 (MQ=255)
                tGCAATCACCGGCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGAcaa                                                                                                                                      >  1:2447673/1‑150 (MQ=255)
                 gCAATCACCGGCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGACGAGCAGACCATCGAcaag                                                                                                                                     >  2:2023441/1‑150 (MQ=255)
                   aaTCACCGGCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGAcaaggc                                                                                                                                   >  1:298006/1‑150 (MQ=255)
                       aCCGGCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGAcaaggccaag                                                                                                                               >  1:2767831/1‑150 (MQ=255)
                         cGGCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGAcaaggccaaggc                                                                                                                             >  1:1866828/1‑150 (MQ=255)
                           gCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCg                                                                                                                           >  2:469726/1‑150 (MQ=255)
                            cAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGa                                                                                                                          <  1:2032831/150‑1 (MQ=255)
                               aCCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACaa                                                                                                                       <  1:1764836/150‑1 (MQ=255)
                                  gATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAg                                                                                                                                                    <  2:2817080/118‑1 (MQ=255)
                                  gATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAg                                                                                                                                                    >  1:2817080/1‑118 (MQ=255)
                                   aTCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTg                                                                                                                   <  1:1763259/150‑1 (MQ=255)
                                    tCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTgg                                                                                                                  <  2:1866828/150‑1 (MQ=255)
                                    tCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCAGACAAGCTgg                                                                                                                  >  2:251391/1‑150 (MQ=255)
                                      aaGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGcc                                                                                                                <  2:1703408/150‑1 (MQ=255)
                                      aaGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGcc                                                                                                                >  2:1610404/1‑150 (MQ=255)
                                          tCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGa                                                                                                            >  1:576698/1‑150 (MQ=255)
                                             tCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAAc                                                                                                         <  1:908200/150‑1 (MQ=255)
                                                 tATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTgaa                                                                                                     <  1:269857/150‑1 (MQ=255)
                                                      ttGAGAAGACCGATGCCATCGAGCCGTTCCAACCCGACCCCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCGCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGaagg                                                                                                <  2:1265437/149‑1 (MQ=255)
                                                      gTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGaagg                                                                                                >  2:2674174/1‑150 (MQ=255)
                                                      gTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGaagg                                                                                                >  2:2599231/1‑150 (MQ=255)
                                                          gaagaCCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGa                                                                                                            >  1:264369/1‑134 (MQ=255)
                                                           aagaCCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGaagggcaag                                                                                           <  2:2245585/150‑1 (MQ=255)
                                                             gaCCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGaagggcaaggg                                                                                         >  1:1973713/1‑150 (MQ=255)
                                                                 gAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAGCGGCAAAGCCAACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTc                                                                                     <  1:2833954/150‑1 (MQ=255)
                                                                 gAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTc                                                                                     >  2:2006391/1‑150 (MQ=255)
                                                                    gCCCTCGGGCCGTTCCACCCCCACCGCGTCGCTTCGCGCATCCTCGGTATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCGGGCCAAGAAACTGAAGTAGGGCAAGGGCTTCGAc                                                                                  <  2:1579020/150‑1 (MQ=255)
                                                                    gCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGAc                                                                                  <  2:2767831/150‑1 (MQ=255)
                                                                       cTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCGCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTGCGACCTc                                                                               >  2:2584912/1‑150 (MQ=255)
                                                                       cTCGAGCCGTTCCACACCGACCGCGTCGCGTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTc                                                                               >  1:729245/1‑150 (MQ=255)
                                                                        tCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCg                                                                              >  2:7887/1‑150 (MQ=255)
                                                                          gAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGaa                                                                            <  1:2102765/150‑1 (MQ=255)
                                                                          gAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGaa                                                                            <  1:1610404/150‑1 (MQ=255)
                                                                          gAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGaa                                                                            <  1:737697/150‑1 (MQ=255)
                                                                            gCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGa                                                                          <  1:2791956/150‑1 (MQ=255)
                                                                                 tCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTcc                                                                     <  1:1226048/150‑1 (MQ=255)
                                                                                    aCCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGACGGGCCAGGGCTTCGACCTCGACGACTTCcgcg                                                                  >  2:953820/1‑150 (MQ=255)
                                                                                    aCCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCcgcg                                                                  >  1:951982/1‑150 (MQ=255)
                                                                                    aCCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCcgcg                                                                  >  2:2873545/1‑150 (MQ=255)
                                                                                     ccccGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGAcaaggccaag                                                                                                                               >  2:1668464/1‑88 (MQ=255)
                                                                                     ccccGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGAcaaggccaag                                                                                                                               <  1:1668464/88‑1 (MQ=255)
                                                                                      cccGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAg                                                                           <  1:2114093/139‑1 (MQ=255)
                                                                                      cccGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAg                                                                           >  2:2114093/1‑139 (MQ=255)
                                                                                      cccGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGAc                                                                <  2:2447673/150‑1 (MQ=255)
                                                                                      cccGACCGCCTAGATTAGCACATCCGCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGAc                                                                >  2:756049/1‑150 (MQ=255)
                                                                                             gcgTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTc                                                                      <  2:2937704/137‑1 (MQ=255)
                                                                                             gcgTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTc                                                                      >  1:2937704/1‑137 (MQ=255)
                                                                                              cgTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTgca                                                        <  1:2023441/150‑1 (MQ=255)
                                                                                                   cTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCaaa                                                   >  2:607210/1‑150 (MQ=255)
                                                                                                   cTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCaaa                                                   <  1:2006391/150‑1 (MQ=255)
                                                                                                   cTTCGCGCATCCTCGGCATGGGCCACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCaaa                                                   <  2:2065241/150‑1 (MQ=255)
                                                                                                    ttCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAAt                                                  >  1:1967440/1‑150 (MQ=255)
                                                                                                         gcATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGaa                                                                            >  1:910073/1‑119 (MQ=255)
                                                                                                          cATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATgaagaa                                            <  1:2575990/150‑1 (MQ=255)
                                                                                                           atcatcGGCATGGGCGACGTGCACAGGCTGATCGAGCAGGCCGAGCAGACCATCGACAAGACCAAAGCCGACAAGCTGGCCAAGAAAGTGAAGAAGGGCAAGGGATTCGACCTCGAAGACTTCCGAGACCAGCTGCAGCAAATgaagaaa                                           >  2:879925/1‑149 (MQ=255)
                                                                                                               tCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATgg                                       >  2:2010432/1‑150 (MQ=255)
                                                                                                               tCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATgg                                       >  2:911574/1‑150 (MQ=255)
                                                                                                                   cATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACATCCGCGACCAGCTGCAGCAAATGAAGAACATGggcgg                                   >  1:318490/1‑150 (MQ=255)
                                                                                                                     tGGGCGACGTGCTCAGCCTGATCGAGCAGGTCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGcc                                 <  2:1996914/150‑1 (MQ=255)
                                                                                                                      gggCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGaagggc                                                                                              <  1:2909208/88‑1 (MQ=255)
                                                                                                                      gggCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGaagggc                                                                                              >  2:2909208/1‑88 (MQ=255)
                                                                                                                        gCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTgaa                                                                                                     >  1:268211/1‑79 (MQ=255)
                                                                                                                        gCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTgaa                                                                                                     <  2:268211/79‑1 (MQ=255)
                                                                                                                            tctGCTCGGCCTGCTCGATCAGGCTGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGTAGCAAATGAAGAACATGGGCGGCCTcggcgg                          >  2:10829/3‑150 (MQ=255)
                                                                                                                             gTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGCCTCggcggc                         >  2:376560/1‑150 (MQ=255)
                                                                                                                             gTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGCCTCggcggc                         >  2:368959/1‑150 (MQ=255)
                                                                                                                                ctcggCCTGCTCGATCAGGCTGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGaa                                                                            <  1:539723/92‑1 (MQ=38)
                                                                                                                                cTCGGCCTGCTCGATCAGGCTGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGaa                                                                            >  2:539723/1‑96 (MQ=38)
                                                                                                                                cTCGGCCTGCTCGATCAGGCTGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGCCTCGGCGGCCTg                      >  1:455263/1‑150 (MQ=255)
                                                                                                                                 tcggCCTGCTCGATCAGGCTGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTc                                                                                     <  2:1412836/83‑1 (MQ=38)
                                                                                                                                 tcggCCTGCTCGATCAGGCTGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTc                                                                                     >  1:1412836/4‑86 (MQ=38)
                                                                                                                                 tCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGCCTCGGCGGCCTGa                     >  2:2699769/1‑150 (MQ=255)
                                                                                                                                 tCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGCCTCGGCGGCCTGa                     >  2:1971304/1‑150 (MQ=255)
                                                                                                                                   aGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGCCTCGGCGGCCTGATg                   <  1:1186278/150‑1 (MQ=255)
                                                                                                                                   aGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGCCTCGGCGGCCTGATg                   <  1:2699769/150‑1 (MQ=255)
                                                                                                                                     ccTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGCCTCGGCGGCCTGATGGa                 >  2:888871/1‑150 (MQ=255)
                                                                                                                                     ccTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGCCTCGGCGGCCTGATGGa                 >  1:1113819/1‑150 (MQ=255)
                                                                                                                                     ccTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGCCTCGGCGGCCTGATGGa                 >  1:763986/1‑150 (MQ=255)
                                                                                                                                     ccTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGCCTCGGCGGCCTGATGGa                 >  1:1796447/1‑150 (MQ=255)
                                                                                                                                        gATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGCCTcgg                             >  2:536237/1‑135 (MQ=255)
                                                                                                                                        gATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGCCTcgg                             <  1:536237/135‑1 (MQ=255)
                                                                                                                                         aTCGAGCAGGCCGAGCAGACCATCGACTGGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGCCTCGGCGGCCTGATGGagatc             >  1:1617880/1‑146 (MQ=255)
                                                                                                                                         aTCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGCCTCGGCGGCCTGATGGACAAg             >  1:1376338/1‑150 (MQ=255)
                                                                                                                                          tCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGCCTCGGCGGCCTGATGGACAAGc            >  1:3024657/1‑150 (MQ=255)
                                                                                                                                           cGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGCCTCGGCGGCCTGATGGACAAGCt           >  2:1953967/1‑150 (MQ=255)
                                                                                                                                              gCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGCCTCGGCGGCCTGATGGACAAGCTGcc        <  1:888871/150‑1 (MQ=255)
                                                                                                                                                  gCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCATGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTgcagc                                                      >  2:39733/1‑100 (MQ=255)
                                                                                                                                                  gCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCATGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTgcagc                                                      <  1:39733/100‑1 (MQ=255)
                                                                                                                                                    cGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGCCTCGGCGGCCTGATGGACAAGCTGCCGAGCAt  >  1:2692334/1‑150 (MQ=255)
                                                                                                                                                    cGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGCCTCGGCGGCCTGATGGACAAGCTGCCGAGCAt  >  2:2073107/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CTGCGCTGTCGGTACGTGCAATCACCGGCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGCCTCGGCGGCCTGATGGACAAGCTGCCGAGCAT  >  NC_002947/1667813‑1668110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑