BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,843,487	G→T	17.6%	S450* (TCA→TAA)	gacS ←	sensor protein GacS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,843,487	0	G	T	17.6%	364.2 / 43.7	140	S450* (TCA→TAA)	gacS	sensor protein GacS
Reads supporting (aligned to +/- strand): ref base G (62/52); new base T (10/15); total (73/67)
Fisher's exact test for biased strand distribution p-value = 2.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 141 total aligned reads displayed.

GGGTACTGTCGACACCGATCTCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGC  >  NC_002947/1843341‑1843636

gggTACTGTCGACACCGATCTCGCCGCCCATCTGTTCGATAAGGCGCTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTTACa                                                                                                                                                    >  1:2919884/1‑150 (MQ=255)

  gTACTGTCGACACCGATCTCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAgg                                                                                                                                                  >  1:951230/1‑150 (MQ=255)

  gTACTGTCGACACCGATCTCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAgg                                                                                                                                                  <  1:1941521/150‑1 (MQ=255)

      tGTCGACACCGATCTCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGa                                                                                                                                              >  1:1834352/1‑150 (MQ=255)

          gACACCGATCTCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGcc                                                                                                                                          <  2:62269/150‑1 (MQ=255)

           acacCGATCTCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTTACAGGCCGATGCCg                                                                                                                                         <  1:1705053/150‑1 (MQ=255)

           acacCGATCTCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGa                                                                                                                                                      >  2:1496754/1‑137 (MQ=255)

             acCGATCTCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGgt                                                                                                                                       <  1:1999643/150‑1 (MQ=255)

              ccGATCTCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGgtg                                                                                                                                      <  1:1967078/150‑1 (MQ=255)

               cGATCTCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGgtgt                                                                                                                                     >  1:514306/1‑150 (MQ=255)

                gATCTCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTc                                                                                                                                    >  2:2109621/1‑150 (MQ=255)

                    tcGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCg                                                                                                                                         <  1:208006/141‑1 (MQ=255)

                      gccgccCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCAc                                                                                                                              >  2:1182323/1‑150 (MQ=255)

                        cgccCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGc                                                                                                                            <  1:1182323/150‑1 (MQ=255)

                        cgccCATCTGTTCGAGCAGGCGCTTGGAAATCACCAGACCCAGCCCGGCGCCTCCGGGCTTGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGGGGTGACAGGCCGATGCCGGTGTCCTGCACGc                                                                                                                            <  2:1119654/150‑1 (MQ=255)

                           ccATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCAGATGCAGTTTTCCTGCACGCTGa                                                                                                                         >  1:1443479/1‑150 (MQ=255)

                             aTCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATg                                                                                                                       >  1:2495273/1‑150 (MQ=255)

                              ccTGTCCGAGCAGGCGCTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATgc                                                                                                                      <  1:518540/149‑1 (MQ=255)

                                 gTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCa                                                                                                                   >  2:789234/1‑150 (MQ=255)

                                  ttCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTTACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCaa                                                                                                                  >  2:2025783/1‑150 (MQ=255)

                                  ttCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTg                                                                                                                                 <  2:2902110/135‑1 (MQ=255)

                                  ttCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTg                                                                                                                                 >  1:2902110/1‑135 (MQ=255)

                                  ttCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCaa                                                                                                                  <  2:1241303/150‑1 (MQ=255)

                                  ttCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCaa                                                                                                                  <  1:737634/150‑1 (MQ=255)

                                      aTCAGGCGCTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTgc                                                                                                              >  1:2511691/1‑150 (MQ=255)

                                           gcgcTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCa                                                                                                         >  2:1818922/1‑150 (MQ=255)

                                           gcgcTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCACACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCa                                                                                                         >  1:1733939/1‑150 (MQ=255)

                                             gcTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTTACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGt                                                                                                       >  2:2625610/1‑150 (MQ=255)

                                               ttGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGATTAGAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCt                                                                                                     >  1:186058/1‑150 (MQ=255)

                                                 ggAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTTACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTc                                                                                                   <  2:2737918/150‑1 (MQ=255)

                                                 ggAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTc                                                                                                   <  1:2181681/150‑1 (MQ=255)

                                                  gAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGTGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCg                                                                                                  >  2:104631/1‑150 (MQ=255)

                                                   aaaTCACCAGCCCCAGACCGGTGCCGCCGGGCTGGTGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGt                                                                                                 <  1:104631/150‑1 (MQ=255)

                                                   aaaTCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGt                                                                                                 >  1:1593633/1‑150 (MQ=255)

                                                         ccAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTgcgcg                                                                                                           <  1:3067751/134‑1 (MQ=255)

                                                           ggCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCt                                                                                         >  2:2108067/2‑150 (MQ=255)

                                                            gCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCtt                                                                                        >  2:2475621/1‑150 (MQ=255)

                                                               ccAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTc                                                                                             >  1:2963722/1‑142 (MQ=255)

                                                               ccAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTc                                                                                             <  2:2963722/142‑1 (MQ=255)

                                                                cAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAgg                                                                                                                                                  >  2:1041319/1‑88 (MQ=255)

                                                                cAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAgg                                                                                                                                                  <  1:1041319/88‑1 (MQ=255)

                                                                  gACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGTCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCa                                                                                  >  2:2227702/1‑150 (MQ=255)

                                                                       gTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGgcg                                                                             >  2:73646/1‑150 (MQ=255)

                                                                          ccgccgGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCg                                                                                                                                         >  1:319572/1‑87 (MQ=255)

                                                                          ccgccgGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCg                                                                                                                                         <  2:319572/87‑1 (MQ=255)

                                                                            gccgGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATgcgc                                                                                                                    <  2:2045650/106‑1 (MQ=255)

                                                                            gccgGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATgcgc                                                                                                                    >  1:2045650/1‑106 (MQ=255)

                                                                            gccgGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATg                                                                                                                       <  1:596610/103‑1 (MQ=255)

                                                                              cgGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGc                                                                                                                                                 <  2:2798460/75‑1 (MQ=255)

                                                                              cgGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGc                                                                                                                                                 >  1:2798460/1‑75 (MQ=255)

                                                                                 gCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTTACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGc                                                                                               >  2:2882720/1‑122 (MQ=255)

                                                                                     gCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGt                                                               <  2:455085/150‑1 (MQ=255)

                                                                                      cGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTg                                                              <  1:789234/150‑1 (MQ=255)

                                                                                       gggCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGc                                                             <  2:1593633/150‑1 (MQ=255)

                                                                                          ccAGTGAGTTGTCAGTCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCtt                                                          <  2:947997/150‑1 (MQ=255)

                                                                                          ccAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCtt                                                          >  2:2569554/1‑150 (MQ=255)

                                                                                             gTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTcgc                                                       >  2:344956/1‑150 (MQ=255)

                                                                                              tGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCa                                                                                     <  2:301103/119‑1 (MQ=255)

                                                                                              tGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCa                                                                                     >  1:301103/1‑119 (MQ=255)

                                                                                               gAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCgg                                                     <  1:572297/150‑1 (MQ=255)

                                                                                               gAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCgg                                                     <  1:344956/150‑1 (MQ=255)

                                                                                                 gTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGt                                                   <  2:2402284/150‑1 (MQ=255)

                                                                                                  ttGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTg                                                  >  2:185580/1‑150 (MQ=255)

                                                                                                     tCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAAc                                               >  2:1265312/1‑150 (MQ=255)

                                                                                                       aGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTGGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACtt                                             >  2:2058510/1‑150 (MQ=255)

                                                                                                       aGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCacga                                                                      <  2:1095451/125‑1 (MQ=255)

                                                                                                       aGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCacga                                                                      >  1:1095451/1‑125 (MQ=255)

                                                                                                         ccTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGc                                                             <  2:539187/132‑1 (MQ=255)

                                                                                                          cTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTTACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGAt                                          <  1:2625610/150‑1 (MQ=255)

                                                                                                          cTGGCTGAAGGCCTGGAACAGTGTGCACACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGAt                                          <  2:1733939/150‑1 (MQ=255)

                                                                                                             gCTGAAGGCCTGAAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGc                                       <  1:2475621/150‑1 (MQ=255)

                                                                                                               tGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGt                                     >  1:2207212/1‑150 (MQ=255)

                                                                                                                   ggCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATg                                                                 <  2:2424198/118‑1 (MQ=255)

                                                                                                                   ggCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATg                                                                 >  1:2424198/1‑118 (MQ=255)

                                                                                                                   ggCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCt                                 >  2:717826/1‑150 (MQ=255)

                                                                                                                    gCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTg                                >  1:1629607/1‑150 (MQ=255)

                                                                                                                    gCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGAAGGCGTTGCTg                                <  2:2495273/150‑1 (MQ=255)

                                                                                                                      cTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGAc                              <  2:1627770/150‑1 (MQ=255)

                                                                                                                       tGGAACAGTGTGCGCACATCCTGAGGTTACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCt                                 <  2:131124/146‑1 (MQ=255)

                                                                                                                       tGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGAcc                             >  1:2404027/1‑150 (MQ=255)

                                                                                                                       tGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGAcc                             >  1:699350/1‑150 (MQ=255)

                                                                                                                         gAACAGTGTGCGCACATCCTGAGGTTACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAg                           >  1:1554093/1‑150 (MQ=255)

                                                                                                                            cAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGtt                        >  2:2934959/1‑150 (MQ=255)

                                                                                                                             aGTGTGCGCACATCCTGAGGTTACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTg                       >  1:2882413/1‑150 (MQ=255)

                                                                                                                              gtgtGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTgg                      >  1:1282366/1‑150 (MQ=255)

                                                                                                                               tgtgCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGt                     >  1:625732/1‑150 (MQ=255)

                                                                                                                                   cgcACATCCTGAGGTTACAGGCCGATGCCGTTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAgg                 <  1:2663291/150‑1 (MQ=255)

                                                                                                                                    gcACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTGGCTGACCAGGTTGGTGAGGa                >  2:574949/1‑150 (MQ=255)

                                                                                                                                     cacaTCCTGAGGTTACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGAt               <  1:698954/150‑1 (MQ=255)

                                                                                                                                      acaTCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATc              >  2:2932946/1‑150 (MQ=255)

                                                                                                                                      acaTCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATc              >  2:1393712/1‑150 (MQ=255)

                                                                                                                                         tCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGc           <  1:2932946/150‑1 (MQ=255)

                                                                                                                                           cTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTcc                                                                                      >  1:1407239/1‑73 (MQ=255)

                                                                                                                                             gAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGa       <  2:618829/150‑1 (MQ=255)

                                                                                                                                              aGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCgg                                                     <  1:1478568/103‑1 (MQ=255)

                                                                                                                                              aGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCgg                                                     >  2:1478568/1‑103 (MQ=255)

                                                                                                                                               ggTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGc     >  2:2988662/1‑150 (MQ=255)

                                                                                                                                               ggTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGc     <  2:625732/150‑1 (MQ=255)

                                                                                                                                               ggTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGc     <  1:2569554/150‑1 (MQ=255)

                                                                                                                                                 tGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCg   <  2:2616500/150‑1 (MQ=255)

GGGTACTGTCGACACCGATCTCGCCGCCCATCTGTTCGATCAGGCGCTTGGAAATCACCAGCCCCAGACCGGTGCCGCCGGGCTGGCGGGCCAGTGAGTTGTCAGCCTGGCTGAAGGCCTGGAACAGTGTGCGCACATCCTGAGGTGACAGGCCGATGCCGGTGTCCTGCACGCTGATGCGCAACTGCGCGCAGTCTTCGTGCTCGTCTTCCAGCATGGCGCGCACGACGATGGTGCCTTCGCGGGTGAACTTGATGGCGTTGCTGACCAGGTTGGTGAGGATCTGCTTGAGCCGC  >  NC_002947/1843341‑1843636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`