BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,693,853	T→C	5.6%	V34A (GTC→GCC)	PP_2362 →	usher protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,693,853	0	T	C	5.6%	319.0 / 5.9	106	V34A (GTC→GCC)	PP_2362	usher protein
Reads supporting (aligned to +/- strand): ref base T (52/48); new base C (3/3); total (55/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.75e-01

Only 100 of 109 total aligned reads displayed.

CAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGATACCGGTGCAGCAGCGGGCCGGGCGGTTGT  >  NC_002947/2693705‑2694002

cAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTc                                                                                                                                                       >  2:395371/1‑150 (MQ=255)

     cTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGccc                                                                                                                                                  >  2:1831378/1‑150 (MQ=255)

        ggcggACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGACCATGGCCAGccccag                                                                                                                                               <  1:1357021/150‑3 (MQ=255)

          cggACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCgcgc                                                                                                                                             >  1:941502/1‑150 (MQ=255)

            gACGCCATCCGCCAAGGCCAATGACCCAACGCCAGCAATTAGCGCACGGTCCATTTATGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTg                                                                                                                                           <  1:2214520/150‑1 (MQ=255)

             aCGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTgg                                                                                                                                          <  2:941502/150‑1 (MQ=255)

               gCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTa                                                                                                                                        >  2:835486/1‑150 (MQ=255)

                ccATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTCCATGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTAc                                                                                                                                       <  1:807217/150‑1 (MQ=255)

                 cATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGGGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACt                                                                                                                                      >  1:940737/1‑150 (MQ=255)

                  aTCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTg                                                                                                                                     >  1:619862/1‑150 (MQ=255)

                   tCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTgg                                                                                                                                    <  2:2866971/150‑1 (MQ=255)

                     cGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGcc                                                                                                                                  >  1:1662894/1‑150 (MQ=255)

                      gCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCcg                                                                                                                                 >  2:1239648/1‑150 (MQ=255)

                        cAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTg                                                                                                                                     <  1:1655871/144‑1 (MQ=255)

                        cAAGGCCAAGGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTg                                                                                                                                     >  2:1655871/1‑144 (MQ=255)

                             ccAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑tt                                                                                                                         <  2:3052549/150‑1 (MQ=255)

                              cAATGAGTCAACGGCCGAATGAAGTGCAGGAGCCAGGGCGAACCGAAAGATGGCTGGAAACCGTGTGGGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTg                                                                                                                        >  1:59145/1‑150 (MQ=255)

                              cAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGACCATGGCCAGCCCcg                                                                                                                                                >  1:142736/1‑127 (MQ=255)

                               aaTGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCCCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑tttc                                                                                                                       >  2:619037/1‑148 (MQ=255)

                               aaTGAGCCAACGGCAGCATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGc                                                                                                                       >  2:1808193/1‑150 (MQ=255)

                                aTGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGcc                                                                                                                      >  1:3012067/1‑150 (MQ=255)

                                    gCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCa                                                                                                                  >  2:768954/1‑150 (MQ=255)

                                     ccAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGcc                                                                                                                                  >  1:752616/1‑134 (MQ=255)

                                     ccAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGcc                                                                                                                                  <  2:752616/134‑1 (MQ=255)

                                     ccAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGccc                                                                                                                     <  2:2511916/146‑1 (MQ=255)

                                     ccAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGccc                                                                                                                     >  1:2511916/1‑146 (MQ=255)

                                        aCGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGc                                                                                                              <  2:59145/150‑1 (MQ=255)

                                          ggCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGccc                                                                                                            <  2:1662894/150‑1 (MQ=255)

                                           gCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCa                                                                                                           >  2:2683084/1‑150 (MQ=255)

                                            ccGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCAc                                                                                                          <  1:1239648/150‑1 (MQ=255)

                                                aTGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGgag                                                                                                      >  1:515521/1‑150 (MQ=255)

                                                  gAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGAt                                                                                                    <  2:2351883/150‑1 (MQ=255)

                                                     gTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTc                                                                                                 >  2:2603098/1‑150 (MQ=255)

                                                       gCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCg                                                                                               <  2:1918944/150‑1 (MQ=255)

                                                            ggCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGcagcg                                                                                          >  2:2192268/1‑146 (MQ=255)

                                                            ggCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATc                                                                                          >  1:1261067/1‑150 (MQ=255)

                                                                aGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCg                                                                                      >  2:2785196/1‑150 (MQ=255)

                                                                      gaCCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCAGGCGACGCTGTACCGCTGGTGCCTGGGGCTGTCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGccc                                                                                >  1:2068367/1‑149 (MQ=255)

                                                                       aCCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCAc                                                                               <  2:2068367/150‑1 (MQ=255)

                                                                         ccGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGCGATGTCCGCCATCGCCGAGGCCAccc                                                                             >  1:2073848/1‑150 (MQ=255)

                                                                         ccGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCAccc                                                                             >  2:398142/1‑150 (MQ=255)

                                                                           ggCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCtt                                                                           <  1:2603098/150‑1 (MQ=255)

                                                                                   ttGGAAATCTTTTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGa                                                                   <  2:778387/150‑1 (MQ=255)

                                                                                   ttGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGa                                                                   >  1:1953351/1‑150 (MQ=255)

                                                                                   ttGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGa                                                                   >  1:1347737/1‑150 (MQ=255)

                                                                                        aaCCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCtgg                                                              <  2:1261067/150‑1 (MQ=255)

                                                                                         aCCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACCGGCCGACGACATATGCCACACC‑CGGGCCTACAGGTCGGCCATCGCCGAGGCCACCCTTTACCTCGACCtggt                                                             <  1:835486/150‑1 (MQ=255)

                                                                                           cGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCtggtgg                                                           <  1:1808193/150‑1 (MQ=255)

                                                                                            gtgtgtGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCACCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCAACCCTTTACCTCGCCCtggtgtt                                                          >  2:353102/1‑148 (MQ=255)

                                                                                             tgtgtgAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGgtggtg                                                         <  1:2683084/150‑1 (MQ=255)

                                                                                                gtgAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCATTTACCTCGACCTGGTGGTGAAc                                                      >  2:456347/1‑150 (MQ=255)

                                                                                                  gAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCa                                                    >  2:2669102/1‑150 (MQ=255)

                                                                                                   aaaTGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGc                                                                                                                       <  2:733506/82‑1 (MQ=255)

                                                                                                   aaaTGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGc                                                                                                                       >  1:733506/1‑82 (MQ=255)

                                                                                                   aaaTGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAg                                                   >  2:1052285/1‑150 (MQ=255)

                                                                                                    aaTGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGa                                                  >  2:2746978/1‑150 (MQ=255)

                                                                                                     agcggtCCCCGCTACGACGTCCCGCTGCTGCCTGGGGCTGGCCATGTTCAGCCTCGCGCTTGTATTGGCCGACTAC‑TTGCCCCCACTGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGAt                                                 <  2:224937/145‑1 (MQ=255)

                                                                                                     aTGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGAt                                                 >  1:2231192/1‑150 (MQ=255)

                                                                                                       ggCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGc                                               <  1:250823/150‑1 (MQ=255)

                                                                                                          tCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGa                                                                                     >  1:632925/1‑109 (MQ=255)

                                                                                                          tCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCa                                            >  1:2186359/1‑150 (MQ=255)

                                                                                                          tCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCa                                            >  2:684387/1‑150 (MQ=255)

                                                                                                          tCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCa                                            >  2:1589440/1‑150 (MQ=255)

                                                                                                            ccGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCg                                                                                      >  1:847697/1‑106 (MQ=255)

                                                                                                            ccGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCg                                                                                      <  2:847697/106‑1 (MQ=255)

                                                                                                            ccGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAgg                                          <  2:515521/150‑1 (MQ=255)

                                                                                                             cGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAggg                                         >  1:1112365/1‑150 (MQ=255)

                                                                                                              ggCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCG‑CATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGtt                                       <  1:2192268/150‑1 (MQ=255)

                                                                                                               gCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGACCGCGGAGATGTCCGACATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGtt                                       <  1:255304/150‑1 (MQ=255)

                                                                                                                cgacgaCGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTg                                      <  1:981841/150‑1 (MQ=255)

                                                                                                                  acgacgTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGAc                                    <  1:684387/150‑1 (MQ=255)

                                                                                                                   cgacgTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGAcc                                   <  1:1247754/150‑1 (MQ=255)

                                                                                                                      cgTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGGCCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGa                                <  2:2073848/150‑1 (MQ=255)

                                                                                                                      cgTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGa                                <  2:2312667/150‑1 (MQ=255)

                                                                                                                      cgTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGa                                <  2:940737/150‑1 (MQ=255)

                                                                                                                       gTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGAt                               <  1:2746978/150‑1 (MQ=255)

                                                                                                                         gCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGATAc                             <  2:3012067/150‑1 (MQ=255)

                                                                                                                          ccGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGTTGAACCAGATGCCCAGGGTTGACCTGATAcc                            >  2:1706644/1‑150 (MQ=255)

                                                                                                                           cGCTTGGGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGATACCg                           >  2:824481/1‑150 (MQ=255)

                                                                                                                             cTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCg                                                                    <  1:1886397/107‑1 (MQ=255)

                                                                                                                             cTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCg                                                                    >  2:1886397/1‑107 (MQ=255)

                                                                                                                               ggTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTTCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGATACCGGTgc                       <  1:619037/150‑1 (MQ=255)

                                                                                                                                cagcgcggggCTGACCATGGCCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGATACCGGTgca                      >  1:2607747/7‑150 (MQ=255)

                                                                                                                                      ggggCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGATACCGGTGCAGCAGcg                <  1:2179641/150‑1 (MQ=255)

                                                                                                                                        ggCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGATACCGGTGCAGCAGcggg              >  1:619246/1‑150 (MQ=255)

                                                                                                                                        ggCTGGCAATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGGGAACCAGATGCCCAGGGTTGACCTGATACCGGTGCAGCAGcggg              >  2:373120/1‑150 (MQ=255)

                                                                                                                                         gCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGATACCGGTGCAGCAGcgggc             >  1:332676/1‑150 (MQ=255)

                                                                                                                                             gCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGATACCGGTGCAGCAGcgggccggg         >  1:2032551/1‑150 (MQ=255)

                                                                                                                                             accATGGCCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGAt                               <  1:2434875/127‑1 (MQ=255)

                                                                                                                                             accATGGCCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGAt                               >  2:2434875/2‑128 (MQ=255)

                                                                                                                                               cATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCGCgg                                                                                                        <  2:1189606/53‑1 (MQ=255)

                                                                                                                                               cATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCGCgg                                                                                                        >  1:1189606/1‑53 (MQ=255)

                                                                                                                                               cATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCtggtg                                                            <  1:828087/97‑1 (MQ=255)

                                                                                                                                               cATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCtggtg                                                            >  2:828087/1‑97 (MQ=255)

                                                                                                                                               cATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGATACCGGTGCAGCAGCGGGCCGGGCg       <  1:2785196/150‑1 (MQ=255)

                                                                                                                                               cATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGATACCGGTGCAGCAGCGGGCCGGGCg       <  1:1052285/150‑1 (MQ=255)

                                                                                                                                                  ggTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGATACCGGTGCAGCAGCGGGCCGGGCGGtt    <  1:61731/150‑1 (MQ=255)

                                                                                                                                                   gTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGATACCGGTGCAGCAGCGGGCCGGGCGGTTg   >  1:2577676/1‑150 (MQ=255)

                                                                                                                                                    tCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCTGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGATACCGGTGCAGCAGCGGGCCGGGCGGTTGt  >  1:2211567/1‑150 (MQ=255)

                                                                                                                                                    tCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGATACCGGTGCAGCAGCGGGCCGGGCGGTTGt  >  2:402818/1‑150 (MQ=255)

CAACACTCGGCGGACGCCATCCGCCAAGGCCAATGAGCCAACGGCCGAATGAAGTGCAGGGGCCAGGGAGGACCCGGCAATGGTTGGAAACCGTGTGTGAAATGGCTCCCGGCGACGACGTGCCGCTGGTGCCTGGGGCTGGCCATGGTCAGCCCCGCGCTGGTACTGGCCGACGAC‑TTGCCCCCACCGCCCACGGAGATGTCCGCCATCGCCGAGGCCACCCTTTACCTCGACCTGGTGGTGAACCAGATGCCCAGGGTTGACCTGATACCGGTGCAGCAGCGGGCCGGGCGGTTGT  >  NC_002947/2693705‑2694002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`