Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,065,582 A→T 5.7% E160V (GAG→GTG)  PP_2677 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,065,5820AT 5.7% 323.6 / 9.5 105E160V (GAG→GTG) PP_2677hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (45/54);  new base T (3/3);  total (48/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.14e-01

Only 100 of 118 total aligned reads displayed.

GTCGATCCGCATCCGCATCGAACAGGCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGTTCTTTCTCAACCATGCCGAGTTACAAGGTGCAGATG  >  NC_002947/3065433‑3065731
                                                                                                                                                     |                                                                                                                                                     
gTCGATCCGCATCCGCATCGAACAGGCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGgga                                                                                                                                                       <  1:2606509/150‑1 (MQ=255)
 tCGATCCGCATCCGCATCGAACAGGCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCCCGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGggag                                                                                                                                                      <  1:201791/150‑1 (MQ=255)
 tCGATCCGCATCCGCATCGAACAGGCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGggag                                                                                                                                                      >  1:1167143/1‑150 (MQ=255)
 tCGATCCGCATCCGCATCGAACAGGCCACACCGCTGCGCCCAGTTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGggag                                                                                                                                                      <  2:966763/150‑1 (MQ=255)
  cGATCCGCATCCGCATCGAACAGGCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGggagg                                                                                                                                                     <  1:448911/150‑1 (MQ=255)
  cGATCCGCATCCGCATCGAACAGGCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGggagg                                                                                                                                                     <  2:2078244/150‑1 (MQ=255)
     tccgcatccgcaTCGAACAGGCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGc                                                                                                                                                  <  1:2340270/150‑1 (MQ=255)
            ccgcatcGAACAGGCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCgg                                                                                                                                           >  2:2878795/1‑150 (MQ=255)
             cgcatcGAACAGGCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCggg                                                                                                                                          <  2:1206968/150‑1 (MQ=255)
              gcatcGAACAGGCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCCCgggg                                                                                                                                         >  1:2346793/1‑150 (MQ=255)
               catcGAACAGGCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGa                                                                                                                                        <  1:1786086/150‑1 (MQ=255)
               catcGAACAGGCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATCGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGa                                                                                                                                        <  1:1005259/150‑1 (MQ=255)
                 tcGAACAGGCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAgg                                                                                                                                      <  2:1937340/150‑1 (MQ=255)
                    aaCAGGCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGc                                                                                                                                   >  2:108069/1‑150 (MQ=255)
                    aaCAGGCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGc                                                                                                                                   >  2:2723999/1‑150 (MQ=255)
                     aCAGGCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCt                                                                                                                                  <  1:2723999/150‑1 (MQ=255)
                      cAGGCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTg                                                                                                                                 <  1:20430/150‑1 (MQ=255)
                        ggCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTggg                                                                                                                               >  1:1400355/1‑150 (MQ=255)
                           cacaCCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGc                                                                                                                                                  >  2:1784044/1‑128 (MQ=255)
                           cacaCCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGc                                                                                                                                                  <  1:1784044/128‑1 (MQ=255)
                           cacaCCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTgggcgg                                                                                                                            <  1:2370780/150‑1 (MQ=255)
                           cacaCCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTgggcgg                                                                                                                            <  2:3066985/150‑1 (MQ=255)
                            acacCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTgggcggg                                                                                                                           >  1:1950715/1‑150 (MQ=255)
                             cacCGCTGCGCGCCTCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTgggcgggc                                                                                                                          <  1:629962/150‑1 (MQ=255)
                              acCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGggcgggcg                                                                                                                         >  1:400746/1‑150 (MQ=255)
                                 gCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTa                                                                                                                      >  1:2060285/1‑150 (MQ=255)
                                        gccgcTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGt                                                                                                               >  1:2106794/1‑150 (MQ=255)
                                          cgcTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTgg                                                                                                             >  2:472927/1‑150 (MQ=255)
                                           gcTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGc                                                                                                            <  1:1900092/150‑1 (MQ=255)
                                             tGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGa                                                                                                          >  2:1798426/1‑150 (MQ=255)
                                               tACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGAca                                                                                                        <  2:769028/150‑1 (MQ=255)
                                               tACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGAca                                                                                                        <  2:698973/150‑1 (MQ=255)
                                                  tGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGAcagca                                                                                                     >  1:12955/1‑150 (MQ=255)
                                                   gACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCCCCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGCGCTGGGTGGGCGCTCCCCGCGTGGCGAcagcag                                                                                                    <  2:2346793/150‑1 (MQ=255)
                                                     cgcgcgATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAgcc                                                                                                  <  1:1291987/150‑1 (MQ=255)
                                                        gcgATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAgccgcc                                                                                               >  2:1354489/1‑150 (MQ=255)
                                                          gATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCcgcctg                                                                                             >  2:1912752/1‑150 (MQ=255)
                                                            tGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCcgcctgcg                                                                                           >  1:1290869/1‑150 (MQ=255)
                                                               ccTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCcgcctgcgcct                                                                                        >  1:2326699/1‑150 (MQ=255)
                                                                cTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCcgcctgcgcctg                                                                                       >  1:2568209/1‑150 (MQ=255)
                                                                cTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCcgcctgcgcctg                                                                                       >  1:1329413/1‑150 (MQ=255)
                                                                       aCGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTgg                                                                                >  2:1385362/1‑150 (MQ=255)
                                                                       aCGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTgg                                                                                <  1:1266435/150‑1 (MQ=255)
                                                                         gTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGcc                                                                              <  1:1076409/150‑1 (MQ=255)
                                                                         gTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGcc                                                                              <  1:2683124/150‑1 (MQ=255)
                                                                         gTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCCCCGAGGTGCtcc                                                                                                                                >  1:1387150/1‑98 (MQ=255)
                                                                          tCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGccc                                                                             <  1:3009240/150‑1 (MQ=255)
                                                                             gTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCAcc                                                                          >  1:2723344/1‑150 (MQ=255)
                                                                                cGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCa                                                                       <  2:1167143/150‑1 (MQ=255)
                                                                                 gACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCCCCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCAt                                                                      <  2:1950715/150‑1 (MQ=255)
                                                                                 gACCCGGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCAt                                                                      <  2:1331972/150‑1 (MQ=255)
                                                                                  aCCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATg                                                                     <  2:1090217/150‑1 (MQ=255)
                                                                                    ccTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCTTGGCGACAGCAGCCGCCTGCGCCTGCAGGTTGCCCACCCCATGGa                                                                   >  2:646419/1‑150 (MQ=255)
                                                                                     cTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGgga                                                                                                                                                       <  2:2069628/65‑1 (MQ=255)
                                                                                     cTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGgga                                                                                                                                                       >  1:2069628/1‑65 (MQ=255)
                                                                                      tGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCCGGTGGCCCCCCCCATGGATa                                                                 >  2:1257805/1‑150 (MQ=255)
                                                                                       gATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATaa                                                                >  1:901543/1‑150 (MQ=255)
                                                                                        aTCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGgaggag                                                                                                                                                   >  1:989908/1‑66 (MQ=255)
                                                                                         tCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGAGCACCCAGCCCGGCTGCAAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTTGCCCACCCCATGGATAACg                                                              <  2:873165/150‑1 (MQ=255)
                                                                                         tCGATGCGGCCGGCGGCGGTTGGACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACg                                                              <  1:2459546/150‑1 (MQ=255)
                                                                                           gATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGc                                                            <  1:2878795/150‑1 (MQ=255)
                                                                                            aTGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGcc                                                           >  2:797589/1‑150 (MQ=255)
                                                                                              gCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTg                                                         <  2:400746/150‑1 (MQ=255)
                                                                                              gCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTg                                                         <  2:1887909/150‑1 (MQ=255)
                                                                                                ggccggcGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGt                                                       <  2:2411474/150‑1 (MQ=255)
                                                                                                        gcggTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCcgcctgcgcct                                                                                        >  1:1732729/1‑109 (MQ=255)
                                                                                                        gcggTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCcgcctgcgcct                                                                                        <  2:1732729/109‑1 (MQ=255)
                                                                                                               tACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTgg                                        <  1:108069/150‑2 (MQ=255)
                                                                                                                   gCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTg                                                                                                              >  2:2404653/1‑76 (MQ=255)
                                                                                                                      cccAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGttcttt                                 <  1:1205340/150‑1 (MQ=255)
                                                                                                                      cccAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGttcttt                                 <  1:2752449/150‑1 (MQ=255)
                                                                                                                       ccAGCGTGGTGCGCACCCCGACCGGCTGGGAGGCGCCCCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGttctttc                                <  2:2174347/150‑1 (MQ=255)
                                                                                                                       ccAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGttctttc                                >  2:1415280/1‑150 (MQ=255)
                                                                                                                       ccAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGttctttc                                >  2:2748322/1‑150 (MQ=255)
                                                                                                                           ggtggtgCGCACCCAGCCCGGGGGGGAGGAGCACCTCGGTGAGGTGCTGGGCGGGGGCTACCCGCGTGGCCAGAGCGGCCGCCTGTGCGTGCAGGTGGCCCTCCGCATGGGTAAAGACCTGGTGAGCCGCATTCCTGAGTTCTTTCTCaa                            <  2:248782/149‑1 (MQ=255)
                                                                                                                               gtgCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCAGGGATAACGGCGTGGTCAGCGGCATTCCTGAGTTCTTTCTCAACCAt                        <  1:472927/150‑1 (MQ=255)
                                                                                                                                 gcgcACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGt                                                                                                                                     >  1:1651495/1‑39 (MQ=255)
                                                                                                                                 gcgcACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGt                                                                                                                                     <  2:1651495/39‑1 (MQ=255)
                                                                                                                                 gcgcACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGGGTTCTTTCTCAACCATGc                      >  1:1365031/1‑150 (MQ=255)
                                                                                                                                 ctcctcccAGCCGGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGTCTGTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGTTCTTTCTCAACCATGc                      >  1:1725635/6‑150 (MQ=255)
                                                                                                                                  tcctcccAGCCGGGCTGGGTGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGttcttt                                 <  1:1216713/134‑1 (MQ=255)
                                                                                                                                  tcctcccAGCCGGGCTGGGTGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGttcttt                                 >  2:1216713/5‑138 (MQ=255)
                                                                                                                                   cctcccAGCCGGGCTGGGTGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCt                                                          <  1:5517/109‑1 (MQ=255)
                                                                                                                                   cctcccAGCCGGGCTGGGTGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCt                                                          >  2:5517/4‑112 (MQ=255)
                                                                                                                                    cACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGTTCTTTCTCAACCATGCCGa                   <  2:2060285/150‑1 (MQ=255)
                                                                                                                                    cACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGTTCTTTCTCAACCATGCCGa                   <  2:2723344/150‑1 (MQ=255)
                                                                                                                                     aCCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGgcgc                                                                                                                        >  2:1495008/1‑48 (MQ=255)
                                                                                                                                     aCCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGgcgc                                                                                                                        <  1:1495008/48‑1 (MQ=255)
                                                                                                                                     aCCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGTTCTTTCTCAACCATGCCGAg                  <  2:2100052/150‑1 (MQ=255)
                                                                                                                                      cccAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGTTCTTTCTCAACCATGCCGAGt                 <  2:2213815/150‑1 (MQ=255)
                                                                                                                                       ccAGCCGGGCTGGGTGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGcc                                                           <  2:1525427/107‑1 (MQ=255)
                                                                                                                                       ccAGCCGGGCTGGGTGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGcc                                                           >  1:1525427/1‑107 (MQ=255)
                                                                                                                                       ccAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATatc                                                               <  1:2892016/103‑3 (MQ=255)
                                                                                                                                       ccAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGa                                        >  2:2921378/1‑126 (MQ=255)
                                                                                                                                         aGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGTTCTTTCTCAACCATGCCGAGTTAc              >  1:1564072/1‑150 (MQ=255)
                                                                                                                                         aGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTCCACTTGCGCTCTGTCGACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGTTCTTTCTCAACCATGCCGAGTTAc              >  2:1782366/1‑150 (MQ=255)
                                                                                                                                             cGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGtt                                     >  2:2430586/1‑123 (MQ=255)
                                                                                                                                             cGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGtt                                     <  1:2430586/123‑1 (MQ=255)
                                                                                                                                                 tGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGTTCTTTCTCAACCATGCCGAGTTACAAGGTGCa      <  1:2169265/150‑1 (MQ=255)
                                                                                                                                                    gaggagCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGTTCTTTCTCAACCATGCCGAGTTACAAGGTGCAGAt   >  1:2452783/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTCGATCCGCATCCGCATCGAACAGGCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGTTCTTTCTCAACCATGCCGAGTTACAAGGTGCAGATG  >  NC_002947/3065433‑3065731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑