Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,204,801 T→C 5.5% M21T (ATG→ACG)  PP_2811 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,204,8010TC 5.5% 275.8 / 3.8 90M21T (ATG→ACG) PP_2811hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (45/40);  new base C (3/2);  total (48/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.28e-01

AACCCTCGCCTGAAGCGCGACTTCTTCACCTCGGATAACCTGCGAGCCATCAGCGAGCGCTAAAACCGACGTCAGATCGAGAGCCAGCATGAAAAAAATAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGTACGGAGTCGCGGGGCTGCAACCTGATCAACCAGGGGCCGACCGGGCACAACGTGCCCTCCGGCTGCCTGGCGCAGA  >  NC_002947/3204652‑3204950
                                                                                                                                                     |                                                                                                                                                     
aaCCCTCGCCTGAAGCGCGACTTCTTCACCTCGGATAACCTGCGAGCCATCAGCGAGCGCTAAAACCGACGTCAGATCGAGAGCCAGCATGAAAAAAATAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAAt                                                                                                                                                       >  1:1020775/1‑150 (MQ=255)
aaCCCTCGCCTGAAGCGCGACTTCTTCACCTCGGATAACCTGCGAGCCATCAGCGAGCGCTAAAACCGACGTCAGATCGAGAGCCAGCATGAAAAAAATAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAAt                                                                                                                                                       >  2:2556399/1‑150 (MQ=255)
aaCCCTCGCCTGAAGCGCGACTTCTTCACCTCGGATAACCTGCGAGCCATCAGCGAGCGCTAAAACCGACGTCAGATCGAGAGCCAGCATGAAAAAAATAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAAt                                                                                                                                                       >  1:869239/1‑150 (MQ=255)
 aCCCTCGCCTGAAGCGCGACTTCTTCACCTCGGATAACCTGCGAGCCATCAGCGAGCGCTAAAACCGACGTCAGATCGAGAGCCAGCATGAAAAAAATAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATg                                                                                                                                                      <  2:2096804/150‑1 (MQ=255)
  cccTCGCCTGAAGCGCGACTTCTTCACCTCGGATAACCTGCGAGCCATCAGCGAGCGCTAAAACCGACGTCAGATCGAGAGCCAGCATGAAAAAAATAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATgg                                                                                                                                                     >  2:2811535/1‑150 (MQ=255)
   ccTCGCCTGAAGCGCGACTTCTTCACCTCGGATAACCTGCGAGCCATCAGCGAGCGCTAAAACCGACGTCAGATCGAGCGCCAGGATGAAAAAAATAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATggg                                                                                                                                                    >  1:54158/1‑150 (MQ=255)
   ccTCGCCTGAAGCGCGACTTCTTCACCTCGGATAACCTGCGAGCCATCAGCGAGCGCTAAAACCGACGTCAGATCGAGAGCCAGCATGAAAAAAATAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATggg                                                                                                                                                    >  2:2377992/1‑150 (MQ=255)
   ccTCGCCTGAAGCGCGACTTCTTCACCTCGGATAACCTGCGAGCCATCAGCGAGCGCTAAAACCGACGTCAGATCGAGAGCCAGCATGAAAAAAATAATTACGAAAGCGTAAGGCCTTTTCATCGTGTGCGCAACACAGTTGATAATggg                                                                                                                                                    >  1:757796/1‑150 (MQ=255)
    cTCGCCTGAAGCGCGACTTCTTCACCTCGGATAACCTGCGAGCCATCAGCGAGCGCTAAAACCGACGTCAGATCGAGAGCCAGCATGAAAAAAATAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATgggg                                                                                                                                                   <  1:1858626/150‑1 (MQ=255)
        ccTGAAGCGCGACTTCTTCACCTCGGATAACCTGCGAGCCATCAGCGAGCGCTAAAACCGACGTCAGATCGAGAGCCAGCATGAAAAAAATAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCa                                                                                                                                               <  2:1852954/150‑1 (MQ=255)
        ccTGAAGCGCGACTTCTTCACCTCGGATAACCTGCGAGCCATCAGCGAGCGCTAAAACCGACGTCAGATCGAGAGCCAGCATGAAAAAAATAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCa                                                                                                                                               <  1:2858720/150‑1 (MQ=255)
         cTGAAGCGCGACTTCTTCACCTCGGATAACCTGCGAGCCATCAGCGAGCGCTAAAACCGACGTCAGATCGAGAGCCAGCATGAAAAAAATAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCAc                                                                                                                                              >  1:1965299/1‑150 (MQ=255)
           gAAGCGCGACTTCTTCACCTCGGATAACCTGCGAGCCATCAGCGAGCGCTAAAACCGACGTCAGATCGAGAGCCAGCATGAAAAAAATAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTc                                                                                                                                                <  1:1093202/146‑1 (MQ=255)
           gAAGCGCGACTTCTTCACCTCGGATAACCTGCGAGCCATCAGCGAGCGCTAAAACCGACGTCAGATCGAGAGCCAGCATGAAAAAAATAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTc                                                                                                                                                >  2:1093202/1‑146 (MQ=255)
              gcgcGACTTCTTCACCTCGGATAACCTGCGAGCCATCAGCGAGCGCTAAAACCGACGTCAGATCGAGAGCCAGCATGAAAAAAATAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTg                                                                                                                                         >  2:446589/1‑150 (MQ=255)
              gcgcGACTTCTTCACCTCGGATAACCTGCGAGCCATCAGCGAGCGCTAAAACCGACGTCAGATCGAGAGCAAGCATGAAAAAAATAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTg                                                                                                                                         >  2:732858/1‑150 (MQ=255)
                 cgACTTCTTCACCTCGGATAACCTGCGAGCCATCAGCGAGCGCTAAAACCGACGTCAGATCGAGAGCCAGCATGAAAAAAATAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTgaaa                                                                                                                                      >  1:1088268/1‑150 (MQ=255)
                       cttcACCTCGGATAACCTGCGAGCCATCAGCGAGCGCTAAAACCGACGTCAGATCGAGAGCCAGCATGAAAAAAATAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTgaaaccgaaa                                                                                                                                <  2:2377669/150‑1 (MQ=255)
                            ccTCGGATAACCTGCGAGCCATCAGCGAGCGCTAAAACCGACGTCAGATCGAGAGCCAGCATGAAAAAAATAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTc                                                                                                                           <  2:1059273/150‑1 (MQ=255)
                             cTCGGATAACCTGCGAGCCATCAGCGAGCGCTAAAACCGACGTCAGATCGAGAGCCAGCATGAAAAAAATAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCa                                                                                                                          >  1:792550/1‑150 (MQ=255)
                             cTCGGATAACCTGCGAGCCATCAGCGAGCGCTAAAACCGACGTCAGATCGAGAGCCAGCATGAAAAAAATAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCa                                                                                                                          >  2:746631/1‑150 (MQ=255)
                              tCGGATAACCTGCGAGCCATCAGCGAGCGCTAAAACCGACGTCAGATCGAGAGCCAGCATGAAAAAAATAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCaa                                                                                                                         >  2:45046/1‑150 (MQ=255)
                              tCGGATAACCTGCGAGCCATCAGCGAGCGATAAATCCTACGTCAGATCGAGAGCCAGCATGAAAAAAATAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCaa                                                                                                                         <  2:1020775/150‑1 (MQ=255)
                               cGGATAACCTGCGAGCCATCAGCGAGCGCTAAAACCGACGTCAGATCGAGAGCCAGCATGAAAAAAATAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAAt                                                                                                                        <  2:1958129/150‑1 (MQ=255)
                               cGGATAACCTGCGAGCCATCAGCGAGCGCTAAAACCGACGTCAGATCGAGAGCCAGCATGAAAAAAATAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAAt                                                                                                                        <  1:2811535/150‑1 (MQ=255)
                                ggATAACCTGCGAGCCATCAGCGAGCGCTAAAACCGACGTCAGATCGAGAGCCAGCATGAAAAAAATAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATg                                                                                                                       <  1:1888851/150‑1 (MQ=255)
                                 gATAACCTGCGAGCCATCAGCGAGCGCTAAAACCGACGTCAGATCGAGAGCCAGCATGAAAAAAATAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATgg                                                                                                                      >  2:777682/1‑150 (MQ=255)
                                  aTAACCTGCGAGCCATCAGCGAGCGCTAAAACCGACGTCAGATCGAGAGCCAGCATGAAAAAAATAATTACGAAAGCGTCATGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATg                                                                                                                                                      >  1:819858/1‑117 (MQ=255)
                                  aTAACCTGCGAGCCATCAGCGAGCGCTAAAACCGACGTCAGATCGAGAGCCAGCATGAAAAAAATAATTACGAAAGCGTCATGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATg                                                                                                                                                      <  2:819858/117‑1 (MQ=255)
                                    acccTTCGAGCCATAAGCGAGCGCTAAACCCGACCTCAGATGGAGAGCCAGCATGAAAAAAATAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTc                                                                                                                   <  1:2160564/148‑1 (MQ=255)
                                    aaCCTGCGAGCCATCAGCGAGCGCTAAAACCGACGTCAGATCGAGAGCCAGCATGAAAAAAATAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTc                                                                                                                   <  1:45046/150‑1 (MQ=255)
                                     aCCTGCGAGCCATCAGCGAGCGCTAAAACCGACGTCAGATCGAGAGCCAGCATGAAAAAAATAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTcc                                                                                                                  <  2:1965299/150‑1 (MQ=255)
                                      ccTGCGAGCCATCAGCGAGCGCTAAAACCGACGTCAGATCGAGAGCCAGCATGAAAAAAATAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCt                                                                                                                 <  2:792550/150‑1 (MQ=255)
                                          cGAGCCATCAGCGAGCGCTAAAACCGACGTCAGATCGAGAGCCAGCATGAAAAAAATAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGa                                                                                                             <  1:777682/150‑1 (MQ=255)
                                           gAGCCATCAGCGAGCGCTAAAACCGACGTCAGATCGAGAGCCAGCATGAAAAAAATAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGa                                                                                                             <  1:821947/149‑1 (MQ=255)
                                           gAGCCATCAGCGAGCGCTAAAACCGACGTCAGATCGAGAGCCAGCATGAAAAAAATAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGa                                                                                                             >  2:821947/1‑149 (MQ=255)
                                              ccATCAGCGAGCGCTAAAACCGACGTCAGATCGAGAGCCAGCATGAAAAAAATAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTgaaaccgaaac                                                                                                                               <  2:1935877/128‑1 (MQ=255)
                                              ccATCAGCGAGCGCTAAAACCGACGTCAGATCGAGAGCCAGCATGAAAAAAATAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTgaaaccgaaac                                                                                                                               >  1:1935877/1‑128 (MQ=255)
                                                aTCAGCGAGCGCTAAAACCGACGTCAGATCGAGAGCCAGCATGAAAAAAATAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTccac                                                                                                       >  1:2606157/1‑150 (MQ=255)
                                                aTCAGCGAGCGCTAAAACCGACGTCAGATCGAGAGCCAGCATGAAAAAAATAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTccac                                                                                                       >  1:2401008/1‑150 (MQ=255)
                                                 tCAGCGAGCGCTAAAACCGACGTCAGATCGAGAGCCAGCATGAAAAAAATAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAGTGGTTCCTTCGACTccacc                                                                                                      >  2:2016607/1‑150 (MQ=255)
                                                     cgagcgCTAAAACCGACGTCAGATCGAGAGCCAGCATGAAAAAAATAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCa                                                                                                  <  1:746631/150‑1 (MQ=255)
                                                                           aTCGAGAGCCAGCATGAAAAAAATAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGTAc                                                                            >  2:846914/1‑150 (MQ=255)
                                                                           aTCGAGAGCCAGCATGAAAAAAATAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGTAc                                                                            <  1:2377992/150‑1 (MQ=255)
                                                                                       cATGAAAAAAATAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGTACgg                                                                          <  2:2629635/140‑1 (MQ=255)
                                                                                       cATGAAAAAAATAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGTACgg                                                                          >  1:2629635/1‑140 (MQ=255)
                                                                                       cATGAAAAAAATAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGTACGGAGTCGCgggg                                                                >  2:1524192/1‑150 (MQ=255)
                                                                                       cATGAAAAAAATAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGTACGGAGTCGCgggg                                                                <  1:2556399/150‑1 (MQ=255)
                                                                                        aTGAAAAAAATAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGTACGGAGTCGCGGGGc                                                               >  2:874833/1‑150 (MQ=255)
                                                                                          gAAAAAAATAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGTACGGAGTCGCGGGGCTg                                                             >  1:745122/1‑150 (MQ=255)
                                                                                           aaaaaaaTAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGTACGGAGTCGCGGGGCTGc                                                            >  1:1093708/1‑150 (MQ=255)
                                                                                           aaaaaaaTAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGTACGGAGTCGCGGGGCTGc                                                            >  2:1268716/1‑150 (MQ=255)
                                                                                             aaaaaTAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGTACGGAGTCGCGGGGCTGCaa                                                          <  1:105109/150‑1 (MQ=255)
                                                                                                 ataatTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGTACGGAGTCGCGGGGCTGCAACCTg                                                      <  1:1268716/150‑1 (MQ=255)
                                                                                                  taatTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGTACGGAGTCGCGGGGCTGCAACCTGa                                                     <  1:2016607/150‑1 (MQ=255)
                                                                                                   aatTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGTACGGAGTCGCGGGGCTGCAACCTGAt                                                    <  2:54158/150‑1 (MQ=255)
                                                                                                     ttACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGTACGGAGTCGCGGGGCTGCAACCTGATCa                                                  >  2:2773492/1‑150 (MQ=255)
                                                                                                     ttACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGTACGGAGTCGCGGGGCTGCAACCTGATCa                                                  >  1:2269274/1‑150 (MQ=255)
                                                                                                          aaaGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGTACGGAGTCGCGGGGCTGCAACCTGATCAACCAg                                             >  2:1997653/1‑150 (MQ=255)
                                                                                                             gCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGTACGGAGTCGCGGGGCTGCAACCTGATCAACCAgggg                                          <  1:1997653/150‑1 (MQ=255)
                                                                                                               gTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGTACGGAGTCGCgg                                                                  >  2:1648833/1‑124 (MQ=255)
                                                                                                               gTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGTACGGAGTCGCgg                                                                  <  1:1648833/124‑1 (MQ=255)
                                                                                                                tCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTcc                                                                                                                  >  2:342596/1‑75 (MQ=255)
                                                                                                                tCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTcc                                                                                                                  <  1:342596/75‑1 (MQ=255)
                                                                                                                  aGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGt                                                                              <  2:2160996/109‑1 (MQ=255)
                                                                                                                  aGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGt                                                                              >  1:2160996/1‑109 (MQ=255)
                                                                                                                    gCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGTACGGAGTCGCGGGGCTGCAACCTGa                                                     >  2:2267206/1‑132 (MQ=255)
                                                                                                                    gCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGTACGGAGTCGCGGGGCTGCAACCTGa                                                     <  1:2267206/132‑1 (MQ=255)
                                                                                                                    gCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGTACGGAGTCGCGGGGCTGCAACCTGATCAACCAGGGGCCGACCg                                   >  2:2062624/1‑150 (MQ=255)
                                                                                                                     ccTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGTACGGAGTCGCGGGGCTGCAACCTGATCAACCAGGGGCCGACCgg                                  >  2:107330/1‑150 (MQ=255)
                                                                                                                     ccTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGTACGGAGTCGCGGGGCTGCAACCTGATCAACCAGGGGCCGACCgg                                  >  1:1564127/1‑150 (MQ=255)
                                                                                                                      cTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGTACGGAGTCGCGGGGCTGCAACCTGATCAACCAGGGGCCGACCggg                                 >  2:2798802/1‑150 (MQ=255)
                                                                                                                      cTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGTACGGAGTCGCGGGGCTGCAACCTGATCAACCAGGGGCCGACCggg                                 <  2:869239/150‑1 (MQ=255)
                                                                                                                      cTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGTACGGAGTCGCGGGGCTGCAACCTGATCAACCAGGGGCCGACCggg                                 >  2:453428/1‑150 (MQ=255)
                                                                                                                        tGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGTACGGAGTCGCgg                                                                  <  2:186303/115‑1 (MQ=255)
                                                                                                                        tGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGTACGGAGTCGCgg                                                                  >  1:186303/1‑115 (MQ=255)
                                                                                                                         gTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGTACGGAGTCGCGGGGCTGCAACCTGATCAACCAGGGGCCGACCGGGcac                              >  1:794731/1‑150 (MQ=255)
                                                                                                                                aaaggtgaaCCCCATTGCCAACGGGGTNCACCTTTGAAACNGAAACCNTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGTACGGAGTCGCGGGGCTGCAACCTGATCAACCAGGGGCCGACCGGGCACAACGTGc                       >  1:2759517/8‑150 (MQ=255)
                                                                                                                                  tgCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGTACGGAGTCGCGGGGCTGCAACCTGATCAACCAGGGGCCGACCGGGCACAACGTGccc                     >  1:1647626/1‑150 (MQ=255)
                                                                                                                                  aggtgaaCCCCATTGCCAACGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGTACGGAGTCGCGGGGCTGCAACCTGATCAAc                                                >  2:2033801/6‑123 (MQ=255)
                                                                                                                                  aggtgaaCCCCATTGCCAACGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGTACGGAGTCGCGGGGCTGCAACCTGATCAAc                                                <  1:2033801/118‑1 (MQ=255)
                                                                                                                                    gtgaaCCCCATTGCCAACGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGTACGGAGTCGCGGGGCTGCAACCTGATCAACCAGGGGCCGAcc                                    >  2:817122/4‑133 (MQ=255)
                                                                                                                                    gtgaaCCCCATTGCCAACGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGTACGGAGTCGCGGGGCTGCAACCTGATCAACCAGGGGCCGAcc                                    <  1:817122/130‑1 (MQ=255)
                                                                                                                                     gcAACCCCGTTGGCCATGGGGGTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGTACGGAGTCGCGGGGCTGCAACCTGATCAACCAGGGGCCGACCGGGCACAACGTGCCCTcc                  >  2:2575347/1‑150 (MQ=255)
                                                                                                                                     gcAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGTACGGAGTCGCGGGGCTGCAACCTGATCAACCAGGGGCCGACCGGGCACAACGTGCCCTcc                  >  1:993033/1‑150 (MQ=255)
                                                                                                                                       aaCCCCGTTGGCAATGGCGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGTACGGAGTCGCGGGCCTGCAACCTGATAAACCAGGTGCCGACCGGGCACGTTGTGcccggtcg                <  2:757796/150‑6 (MQ=255)
                                                                                                                                          cccGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGTACGGAGTCGCGGGGCTGCAACCTGATCAACCAGGGGCCGACCGGGCACAACGTGCCCTCCGGATg             >  2:2805861/1‑150 (MQ=255)
                                                                                                                                           ccGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGTACGGAGTCGCGGGGCTGCAACCTGATCAACCAGGGGCCGACCGGGCACAACGTGCCCTCCGGCTGc            <  1:2062624/150‑1 (MQ=255)
                                                                                                                                              ttGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGTACGGAGTCGCGGGGCTGCAACCTGATCAACCAGGGGCCGACCGGGCACAACGTGCCCTCCGGCTGCCTg         <  2:2606157/150‑1 (MQ=255)
                                                                                                                                                   aaTGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGTACGGAGTCGCGGGGCTGCAACCTGATCAACCAGGGGCCGACCGGGCACAACGTGCCCTCCGGCTGCCTg         >  1:1113750/1‑145 (MQ=255)
                                                                                                                                                   aaTGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGTACGGAGTCGCGGGGCTGCAACCTGATCAACCAGGGGCCGACCGGGCACAACGTGCCCTCCGGCTGCCTg         <  2:1113750/145‑1 (MQ=255)
                                                                                                                                                   aaTGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGTACGGAGTCGCGGGGCTGCAACCTGATCAACCAGGGGCCGACCGGGCACAACGTGCCCTCCGGCTGCCTGGCGCa    <  1:446589/150‑1 (MQ=255)
                                                                                                                                                   aaTGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGTACGGAGTCGCGGGGCTGCAACCTGATCAACCAGGGGCCGACCGGGCACAACGTGCCCTCCGGCTGCCTGGCGCa    <  1:874833/150‑1 (MQ=255)
                                                                                                                                                     tGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGTACGGAGTCGCGGGGCTGCAACCTGATCAACCAGGGGCCGACCGGGCACAACGTGCCCTCCGGCTACCTGGCGCaga  <  2:1088268/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AACCCTCGCCTGAAGCGCGACTTCTTCACCTCGGATAACCTGCGAGCCATCAGCGAGCGCTAAAACCGACGTCAGATCGAGAGCCAGCATGAAAAAAATAATTACGAAAGCGTCAGGCCTTGTCATCGTGTGCGCAACCCCGTTGGCAATGGGGTTCACCTTTGAAACCGAAACCCTCAATGGTTCCTTCGACTCCACCATCACCGCAGGCATGGGCCTGCGTACGGAGTCGCGGGGCTGCAACCTGATCAACCAGGGGCCGACCGGGCACAACGTGCCCTCCGGCTGCCTGGCGCAGA  >  NC_002947/3204652‑3204950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑