BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,688,223	G→T	6.1%	L99L (CTG→CTT)	PP_3253 →	carboxylate‑amine ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,688,223	0	G	T	6.1%	410.3 / 7.6	128	L99L (CTG→CTT)	PP_3253	carboxylate‑amine ligase
Reads supporting (aligned to +/- strand): ref base G (62/58); new base T (4/4); total (66/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

Only 100 of 134 total aligned reads displayed.

GCCAGATCGAGCTGGCGTCGCCGGTGTTCACCAACCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACC‑‑GTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATGCAACTGATCAACCGCCTTCTGC  >  NC_002947/3688075‑3688371

    gATCGAGCTGGCGTCGCCGGTGTTCACCAACCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACC‑‑GTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCa                                                                                                                                                   <  1:486956/150‑1 (MQ=255)

    gATCGAGCTGGCGTCGCCGGTGTTCACCAACCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACC‑‑GTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCa                                                                                                                                                   <  2:1965870/150‑1 (MQ=255)

       cGAGCTGGCGTCGCCGGTGTTCACCAACCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACC‑‑GTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGaa                                                                                                                                                <  1:506640/150‑1 (MQ=255)

            tGGCGTCGCCGGTGTTCACCAACCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACC‑‑GTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑cg                                                                                                                                          >  1:2716002/1‑150 (MQ=255)

                 tCGCCGGTGTTCACCAACCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACC‑‑GTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGca                                                                                                                                     <  1:649136/150‑1 (MQ=255)

                  cGCCGGTGTTCACCAACCTGTATGAAGCACGTGAGTTTCTCCCGTGAAGCCTGGACTACAACTAC‑CG‑GGAAGTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGTGCCGCG‑GGCTTCTGCAGCAGCCACGCGGcgc                                                                                                                                    <  1:1135338/150‑3 (MQ=255)

                   gCCGGTGTTCACCAACCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACC‑‑GTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGaa                                                                                                                                                >  2:1692998/1‑138 (MQ=255)

                   gCCGGTGTCCACCAACCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACC‑‑GTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGaa                                                                                                                                                <  1:1692998/138‑1 (MQ=255)

                      ggTGTTCACCAACCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACC‑‑GTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGTGCCGCG‑GGCTTCTGCAGCAGCCACGCGGcgcaggg                                                                                                                                <  1:2634992/150‑7 (MQ=38)

                        tgtTCACCAACCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACC‑‑GTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGc                                                                                                                              >  1:2540860/1‑150 (MQ=255)

                             aCCAACCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACC‑‑GTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGgctgct                                                                                                                                                      >  2:774259/1‑122 (MQ=255)

                             aCCAACCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACC‑‑GTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACtat                                                                                                                         <  2:1307775/150‑1 (MQ=255)

                                aaCCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACC‑‑GTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAag                                                                                                                      >  2:2601777/1‑150 (MQ=255)

                                 aCCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACC‑‑GTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAagc                                                                                                                     <  1:2049703/150‑1 (MQ=255)

                                   cTGTATGAAGCACGTGAGTTTCTCCAGCGCAACC‑‑GTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAagcag                                                                                                                   <  1:755066/150‑1 (MQ=255)

                                   cTGTATGAAGCACGTGAGTTTCTCCAGCGCAACC‑‑GTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAagcag                                                                                                                   >  1:1956493/1‑150 (MQ=255)

                                        tGAAGCACGTGAGTTTCTCCAGCGCAACC‑‑GTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCAc                                                                                                                            <  2:2481336/136‑1 (MQ=255)

                                        tGAAGCACGTGAGTTTCTCCAGCGCAACC‑‑GTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCAc                                                                                                                            >  1:2481336/1‑136 (MQ=255)

                                        tGAAGCACGTGAGTTTCTCCAGCGCAACC‑‑GTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGtt                                                                                                              <  1:1341940/150‑1 (MQ=255)

                                         gAAGCACGTGAGTTTCTCCAGCGCAACC‑‑GTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATCCGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTc                                                                                                             >  1:1144228/1‑150 (MQ=255)

                                          aaGCACGTGAGTTTCTCCAGCGCAACC‑‑GTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACACGGGCACTATAAGCAGCTGTTcg                                                                                                            >  1:2736351/1‑150 (MQ=255)

                                            gCACGTGAGTTTCTCCAGCGCAACC‑‑GTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTcgac                                                                                                          <  1:863284/150‑1 (MQ=255)

                                            gCACGTGAGTTTCTCCAGCGCAACC‑‑GTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTcgac                                                                                                          >  2:863284/1‑150 (MQ=255)

                                            gCACGTGAGTTTCTCCAGCGCAACC‑‑GTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTcgac                                                                                                          <  2:2419202/150‑1 (MQ=255)

                                              aCGTGAGTTTCTCCAGCGCAACC‑‑GTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATaa                                                                                                                       <  1:2167454/135‑1 (MQ=255)

                                              aCGTGAGTTTCTCCAGCGCAACC‑‑GTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATaa                                                                                                                       >  2:2167454/1‑135 (MQ=255)

                                                gTGAGTTTCTCCAGCGCAACC‑‑GTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACt                                                                                                     >  1:349869/1‑150 (MQ=255)

                                                 tGAGTTTCTCCAGCGCAACC‑‑GTCAGCGGCTGCGCGTGGCATTGGCCGAAGTTGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTa                                                                                                    >  1:1329036/1‑150 (MQ=255)

                                                 tGAGTTTCTCCAGCGCAACC‑‑GTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTa                                                                                                    >  1:1109898/1‑150 (MQ=255)

                                                  gAGTTTCTCCAGCGCAACC‑‑GTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTAc                                                                                                   >  1:1875213/1‑150 (MQ=255)

                                                   aGTTTCTCCAGCGCAACC‑‑GTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTAcc                                                                                                  >  1:2145054/1‑150 (MQ=255)

                                                    gTTTCTCCAGCGCAACC‑‑GTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCg                                                                                                 >  1:1424666/1‑150 (MQ=255)

                                                    gTTTCTCCAGCGCAACC‑‑GTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCGGCAGAGGCC‑CGCGGCACAAGAGCACGATAAGCAGCTGTTCGACG‑ACTACCg                                                                                                 >  2:2039201/1‑150 (MQ=255)

                                                     tttCTCCAGCGCAACC‑‑GTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGc                                                                                                >  2:2401215/1‑150 (MQ=255)

                                                     tttCTCCAGCGCAACC‑‑GTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGc                                                                                                >  2:2064196/1‑150 (MQ=255)

                                                     tttCCCCAGCGCAACC‑‑GTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGc                                                                                                <  1:347766/150‑1 (MQ=255)

                                                         tcCAGCGCAACC‑‑GTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATCCGCTGCGGCAAGCCACCCCTGCGCCGCG‑GGCTTCTGCAGCAGccacgc                                                                                                                                         <  1:1606196/106‑5 (MQ=38)

                                                         tcCAGCGCAACC‑‑GTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGTGCCGCG‑GGCTTCTGCAGCAGCCACGCGGCGCAggggtgg                                                                                                                            <  2:1481949/119‑4 (MQ=25)

                                                         tcCAGCGCAACC‑‑GTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAg                                                                                                                                                  >  1:877407/1‑98 (MQ=255)

                                                         tcCAGCGCAACC‑‑GTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCAtg                                                                                            >  1:1887972/1‑150 (MQ=255)

                                                         tcCAGCGCAACC‑‑GTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGAGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCAtg                                                                                            >  2:1646561/1‑150 (MQ=255)

                                                          ccAGCGCAACC‑‑GTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCAtgt                                                                                           <  2:2540860/150‑1 (MQ=255)

                                                           cAGCGCAACC‑‑GTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCt                                                                                                                 <  1:2686593/128‑1 (MQ=255)

                                                           cAGCGCAACC‑‑GTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCt                                                                                                                 >  2:2686593/1‑128 (MQ=255)

                                                                  aCC‑‑GTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCATGTGGCAAGGc                                                                                   <  2:1887972/150‑1 (MQ=255)

                                                                   cc‑‑GTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCTTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCATGTGGCAAGGCg                                                                                  >  2:2199635/1‑150 (MQ=255)

                                                                       gTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCATGTGGCAAGGCGTa                                                                                >  2:941467/1‑150 (MQ=255)

                                                                         tgacGGTTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCATGTGGCAAGGCGTAGc                                                                              <  2:2791883/147‑1 (MQ=255)

                                                                          aGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCATGTGGCAAGGCGTAGcc                                                                             >  2:431741/1‑150 (MQ=255)

                                                                            cGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCAc                                                                                                                            <  1:955159/102‑1 (MQ=255)

                                                                            cGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCAc                                                                                                                            >  2:955159/1‑102 (MQ=255)

                                                                              gCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCATGTGGCAAGGCGTAGCCTGCt                                                                         <  1:1665892/150‑1 (MQ=255)

                                                                                tGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCATGTGGCAAGGCGTAGCCTGCTCa                                                                       >  1:577502/1‑150 (MQ=255)

                                                                                     gTGGAATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGc                                                                  <  2:851456/150‑1 (MQ=255)

                                                                                      tGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCAtgt                                                                                           <  2:2868130/124‑1 (MQ=255)

                                                                                      tGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCAtgt                                                                                           >  1:2868130/1‑124 (MQ=255)

                                                                                      tGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATg                                                                    <  1:592208/147‑1 (MQ=255)

                                                                                      tGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATg                                                                    >  2:592208/1‑147 (MQ=255)

                                                                                       ggCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCt                                                                >  2:2765138/1‑150 (MQ=255)

                                                                                         cATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGc                                                              >  2:2563027/1‑150 (MQ=255)

                                                                                          aTTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCATGTGGCAAGGCGTa                                                                                <  2:638383/131‑1 (MQ=255)

                                                                                          aTTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCa                                                             <  1:2601777/150‑1 (MQ=255)

                                                                                           ttGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCAt                                                            <  1:2064196/150‑1 (MQ=255)

                                                                                             ggCCGAAGAGGGGATGGGGCCATAAGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGTCTGCAtgt                                                          <  1:8332/150‑1 (MQ=255)

                                                                                               ccGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCa                                                                                                                             >  2:513483/1‑82 (MQ=255)

                                                                                               ccGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGc                                                        >  2:1589886/1‑150 (MQ=255)

                                                                                                 gAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCAc                                                      <  1:2560822/150‑1 (MQ=255)

                                                                                                      ggggATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCAtgt                                                                                           <  1:2909769/108‑1 (MQ=255)

                                                                                                      ggggATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCAtgt                                                                                           >  2:2909769/1‑108 (MQ=255)

                                                                                                       gggATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGc                                                >  2:1855171/1‑150 (MQ=255)

                                                                                                           tGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGGCG‑ACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTgc                                            <  1:236099/150‑1 (MQ=255)

                                                                                                           tGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTgc                                            <  2:469566/150‑1 (MQ=255)

                                                                                                               gCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTgccgcc                                        >  2:428969/1‑150 (MQ=255)

                                                                                                               gCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTgccgcc                                        >  2:1500444/1‑150 (MQ=255)

                                                                                                                ccATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCa                                       >  2:2604466/1‑150 (MQ=255)

                                                                                                                   tACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCt                                    >  1:2667266/1‑150 (MQ=255)

                                                                                                                    aCGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGTAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCtt                                   <  1:2039201/150‑1 (MQ=255)

                                                                                                                    aCGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCATGTGGCAAGGCGTAGCCTg                                                                           <  2:1996589/110‑1 (MQ=255)

                                                                                                                    aCGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCtt                                   <  1:2401215/150‑1 (MQ=255)

                                                                                                                      gCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTtgt                                 <  1:431741/150‑1 (MQ=255)

                                                                                                                        tGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGa                               <  1:501384/150‑1 (MQ=255)

                                                                                                                        tGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGa                               <  1:2253396/150‑1 (MQ=255)

                                                                                                                           ggCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTtgt                                 >  2:1760765/1‑145 (MQ=255)

                                                                                                                           ggCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTtgt                                 <  1:1760765/145‑1 (MQ=255)

                                                                                                                           ggCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCg                            >  1:2779175/1‑150 (MQ=255)

                                                                                                                                 ccACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCAt                         <  2:3027157/147‑1 (MQ=255)

                                                                                                                                 ccACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCAt                         >  1:3027157/1‑147 (MQ=255)

                                                                                                                                     ccttgtgccgcG‑GGCTTCTGCAGCAGCCACGCGGCGC‑AGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATGCAACTGa                 >  1:404791/7‑150 (MQ=37)

                                                                                                                                     cccTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATGCAACTg                  <  1:428969/150‑1 (MQ=255)

                                                                                                                                      cttgtgccgcG‑GGCTTCTGCAGCAGCCACGCGGCGCAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATGCAACTGa                 >  2:1736895/6‑150 (MQ=38)

                                                                                                                                      cttGCGCCGCGTGGCTGCTGCAAAAGCC‑CGGGGCACAAGAGAACTCTACGCAGCAGTTGTCTGTTCTGTCG‑AAGTTGGCAAGGCTTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATGCAACTGa                 <  1:55091/148‑1 (MQ=255)

                                                                                                                                      ccTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATGCAACTGa                 >  2:2809318/1‑150 (MQ=255)

                                                                                                                                      ccTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATGCAACTGa                 <  2:1329036/150‑1 (MQ=255)

                                                                                                                                            ccgcGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATg                                                                    >  2:1759363/1‑93 (MQ=255)

                                                                                                                                            ccgcGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATg                                                                    <  1:1759363/93‑1 (MQ=255)

                                                                                                                                                gTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATGCAACTGATCAAc            >  2:1688681/1‑145 (MQ=255)

                                                                                                                                                 tGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATGCAACTGATCAACCGCCtt      <  2:2507382/150‑1 (MQ=255)

                                                                                                                                                    ctgctgCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATGCAACTGATCAACCGCCTTCTg   <  1:1500444/150‑1 (MQ=255)

                                                                                                                                                     tgctgcAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCATGTGGCAAGGCTGAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATGCAACTGATCAACCGCCTTCTGc  >  1:2518306/1‑150 (MQ=255)

                                                                                                                                                     tgctgcAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATGCAACTGATCAACCGCCTTCTGc  >  2:1843906/1‑150 (MQ=255)

GCCAGATCGAGCTGGCGTCGCCGGTGTTCACCAACCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACC‑‑GTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACG‑ACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATGCAACTGATCAACCGCCTTCTGC  >  NC_002947/3688075‑3688371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`