Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,883,615 C→G 7.1% A197G (GCC→GGC) ‡ PP_3428 → TPR repeat‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,883,6150CG 7.1% 348.3 / 13.8 108A197G (GCC→GGC) ‡PP_3428TPR repeat‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (54/46);  new base G (3/5);  total (57/51)
Fisher's exact test for biased strand distribution p-value = 4.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01

Only 100 of 112 total aligned reads displayed.

GACGCCATCGGCCTCTATTTCGGCCCCCTGCAGTCAGCCCTGGAGCAATACCAGCAAGCCCTGGAGCAACAGCCTGAAGATGCCGACGCCCAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAGGTACGCCTGCTCGAGCGCCAGGGCCACCACGACGCCTCCCGCCAGGTGCTGGGCAAAGCCCTGGCGCTGAGCCCCGACTCGGCCTTTCTGCAGT  >  NC_002947/3883467‑3883764
                                                                                                                                                     |                                                                                                                                                     
gACGCCGTCTGCCTCTATTTCGGCCCCCTGCAGTCAGCCCTGGAGCAATACCAGCAAGCCCTGGAGCAACAGCCTGAAGATGCCGACGCCCAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGcc                                                                                                                                                      <  2:649116/150‑1 (MQ=255)
gACGCCATCGGCCTCTATTTCGGCCCCCTGCAGTCAGCCCTGGAGCAATACCAGCAAGCCCTGGAGCAACAGCCTGAAGATGCCGACGCCCAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGcc                                                                                                                                                      <  1:1194965/150‑1 (MQ=255)
gACGCCATCGGCCTCTATTTCGGCCCCCTGCAGTCAGCCCTGGAGCAATACCAGCAAGCCCTGGAGCAACAGCCTGAAGATGCCGACGCCCAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGcc                                                                                                                                                      >  2:100637/1‑150 (MQ=255)
     cATCGGCCTCTATTTCGGCCCCCTGCAGTCAGCCCTGGAGCAATACCAGCAAGCCCTGGAGCAACAGCCTGAAGATGCCGACGCCCAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCa                                                                                                                                                 <  2:237355/150‑1 (MQ=255)
     cATCGGCCTCTATTTCGGCCCCCTGCAGTCAGCCCTGGAGCAATACCAGCAAGCCCTGGAGCAACAGCCTGAAGATGCCGACGCCCAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCa                                                                                                                                                 <  2:499951/150‑1 (MQ=255)
        cGGCCTCTATTTCGGCCCCCTGCAGTCAGCCCTGGAGCAATACCAGCAAGCCCTGGAGCAACAGCCTGAAGATGCCGACGCCCAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCagcg                                                                                                                                              >  1:2766264/1‑150 (MQ=255)
         ggCCTCTATTTCGGCCCCCTGCAGTCAGCCCTGGAGCAATACCAGCAAGCCCTGGAGCAACAGCCTGAAGATGCCGACGCCCAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCagcga                                                                                                                                             <  1:2706769/150‑1 (MQ=255)
            ctctATTTCGGCCCCCTGCAGTCAGCCCTGGAGCAATACCAGCAAGCCCTGGAGCAACAGCCTGAAGATGCCGACGCCCAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCagcgagcg                                                                                                                                          >  2:1340380/1‑150 (MQ=255)
             tctATTTCGGCCCCCTGCAGTCAGCCCTGGAGCAATACCAGCAAGCCCTGGAGCAACAGCCTGAAGATGCCGACGCCCAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAgcgc                                                                                                                                         >  1:981907/1‑150 (MQ=255)
              ctATTTCGGCCCCCTGCAGTCAGCCCTGGAGCAATACCAGCAAGCCCTGGAGCAACAGCCTGAAGATGCCGACGCCCAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGGGCTGCagcggcgtgc                                                                                                                                        <  2:482271/150‑7 (MQ=255)
              ctATTTCGGCCCCCTGCAGTCAGCCCTGGAGCAATACCAGCAAGCCCTGGAGCAACAGCCTGAAGATGCCGAAGGCTATTTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCt                                                                                                                                        >  1:689355/1‑150 (MQ=255)
               tATTTCGGCCCCCTGCAGTCAGCCCTGGAGCAATACCAGCAAGCCCTGGAGCAACAGCCTGAAGATGCCGACGCCCACGTGCACCTGGCGCGCCTGTCCCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTc                                                                                                                                       <  2:1649896/150‑1 (MQ=255)
                 tttCGGCCCCCTGCAGTCAGCCCTGGAGCAATACCAGCAAGCCCTGGAGCAACAGCCTGAAGATGCCGACGCCCAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTcgc                                                                                                                                     >  1:2913034/1‑150 (MQ=255)
                      gCCCCCTGCAGTCAGCCCTGGAGCAATACCAGCAAGCCCTGGAGCAACAGCCTGAAGATGCCGACGCCCAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTg                                                                                                                                >  2:1186281/1‑150 (MQ=255)
                         cccTGCAGTCAGCCCTGGAGCAATACCAGCAAGCCCTGGAGCAACAGCCTGAAGATGCCGACGCCCAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGccc                                                                                                                             >  1:1340516/1‑150 (MQ=255)
                         cccTGCAGTCAGCCCTGGAGCAATACCAGCAAGCCCTGGAGCAACAGCCTGAAGATGCCGACGCCCAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGccc                                                                                                                             >  2:1654925/1‑150 (MQ=255)
                                 tCAGCCCTGGAGCAATACCAGCAAGCCCTGGAGCAACAGCCTGAAGATGCCGACGCCCAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCgg                                                                                                                                   >  2:271389/1‑136 (MQ=255)
                                  cAGCCCTGGAGCAATACCAGCAAGCCCTGGAGCAACAGCCTGAAGATGCCGACGCCCAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAAcc                                                                                                                    <  2:2727159/150‑1 (MQ=255)
                                   aGCCCTGGAGCAATACCAGCAAGCCCTGGAGCAACAGCCTGAAGATGCCGACGCCCAAGTGCACCTGGTGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCt                                                                                                                   >  2:1760185/1‑150 (MQ=255)
                                   aGCCCTGGAGCAATACAAGCAAGCCCTGTATCAACAGCCTGAAGATGCCGACGCCCAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCt                                                                                                                   >  1:2374364/1‑150 (MQ=255)
                                      ccTGGAGCAATACCAGCAAGCCCTGGAGCAACAGCCTGAAGATGCCGACGCCCAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGa                                                                                                                >  1:1151088/1‑150 (MQ=255)
                                       cTGGAGCAATACCAGCAAGCCCTGGAGCAACAGCCTGAAGATGCCGACGCCCAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGAt                                                                                                               <  1:615742/150‑1 (MQ=255)
                                        tGGAGCAATACCAGCAAGCCCTGGAGCAACAGCCTGAAGATGCCGACGCCCAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCTAGCGCTCGCGGTTGCCCCCGGCAAc                                                                                                                     <  2:465414/143‑1 (MQ=255)
                                           aGCAATACCAGCAAGCCCTGGAGCAACAGCCTGAAGATGCCGACGCCCAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGccc                                                                                                           <  2:2374364/150‑1 (MQ=255)
                                             cAATACCAGCAAGCCCTGGAGCAACAGCCTGAAGATGCCGACGCCCAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTg                                                                                                         >  1:2188130/1‑150 (MQ=255)
                                             cAATACCAGCAAGCCCTGGAGCAACAGAAGAAAGATGCCGACGCCCAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTg                                                                                                         >  1:2860709/1‑150 (MQ=255)
                                              aaTACCAGCAAGCCCTGGAGCAACAGCCTGAAGATGCCGACGCCCAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTgg                                                                                                        >  1:2153576/1‑150 (MQ=255)
                                              aaTACCAGCAAGCCCTGGAGCAACAGCCTGAAGATGCCGACGCCCAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTgg                                                                                                        <  1:1099608/150‑1 (MQ=255)
                                                  ccAGCAAGCCCTGGAGCAACAGCCTGAAGATGCCGACGCCCAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGAc                                                                                                    >  2:2326057/1‑150 (MQ=255)
                                                      cAAGCCCTGGAGCAACAGCCTGAAGATGCCGACGCCCAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGccc                                                                                                           >  1:1230718/1‑139 (MQ=255)
                                                      cAAGCCCTGGAGCAACAGCCTGAAGATGCCGACGCCCAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGccc                                                                                                           <  2:1230718/139‑1 (MQ=255)
                                                      cAAGCCCTGGAGCAACAGCCTGAAGATGCCGACGCCCAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAg                                                                                                <  1:1186281/150‑1 (MQ=255)
                                                      cAAGCCCTGGAGCAACAGCCTGAAGATGCCGACGCCCAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAg                                                                                                <  2:2766264/150‑1 (MQ=255)
                                                              ggAGCAACAGCCTGAAGATGCCGACGCCCAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAGGTACGCCt                                                                                        >  1:1709406/1‑150 (MQ=255)
                                                               gAGCAACAGCCTGAAGATGCCGACGCCCAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAgg                                                                                               <  2:925219/142‑1 (MQ=255)
                                                                    aCAGCCTGAAGATGCCGACGCCCAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAGGTACGCCTGCTCGa                                                                                  <  2:1151088/150‑1 (MQ=255)
                                                                     cAGCCTGAAGATGCCGACGCCCAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCt                                                                                                                                                    <  2:2613609/83‑1 (MQ=255)
                                                                     cAGCCTGAAGATGCCGACGCCCAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCt                                                                                                                                                    >  1:2613609/1‑83 (MQ=255)
                                                                        ccTGAAGATGCCGACGCCCAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGGGCTGCagc                                                                                                                                               >  1:1755102/1‑85 (MQ=255)
                                                                        ccTGAAGATGCCGACGCCCAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGGGCTGCagc                                                                                                                                               <  2:1755102/85‑1 (MQ=255)
                                                                         cTGAAGATGCCGACGCCCAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGtt                                                                                                                                 <  2:2625063/98‑1 (MQ=255)
                                                                         cTGAAGATGCCGACGCCCAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGtt                                                                                                                                 >  1:2625063/1‑98 (MQ=255)
                                                                           gAAGATGCCGACGCCCAAGTGCACATTACCTTCCTTTACCTGCACGAAAACGACTACACGCATCGCC‑CTGACGGCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAGGTACGCCTGCTCGAGCGCCAg                                                                           <  1:55946/150‑1 (MQ=255)
                                                                            aaGATGCCGACGCCCAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTc                                                                                                  >  1:2670186/1‑126 (MQ=255)
                                                                            aaGATGCCGACGCCCAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTc                                                                                                  <  2:2670186/126‑1 (MQ=255)
                                                                            aaGATGCCGACGCCCAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAGGTACGCCTGCTCGAGCGCCAgg                                                                          >  1:1332457/1‑150 (MQ=255)
                                                                            aaGATGCCGACGCCCAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAGGTACGCCTGCTCGAGCGCCAgg                                                                          <  1:2326057/150‑1 (MQ=255)
                                                                            aaGATGCCGACGCCCAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAGGTACGCCTGCTCGAGCGCCAgg                                                                          <  2:2153576/150‑1 (MQ=255)
                                                                            aaGATGCCGACGCCCAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAGGTACGCCTGCTCGAGCGCCAgg                                                                          <  2:211144/150‑1 (MQ=255)
                                                                            aaGATGCCGACGCCCAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGAAGCACTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAGGTACGCCTGCTCGAGCGCCAgg                                                                          >  1:1051979/1‑150 (MQ=255)
                                                                              gATGCCGACGCCCAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAAc                                                                                                                     >  2:447221/1‑105 (MQ=255)
                                                                              gATGCCGACGCCCAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAAc                                                                                                                     <  1:447221/105‑1 (MQ=255)
                                                                                       gCCCAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAGGTACGCCTGCTCGAGCGCCAGGGCCACCacgac                                                               >  1:2094475/1‑150 (MQ=255)
                                                                                         ccAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAGGTACGCCTGCTCGAg                                                                                 >  1:470302/1‑130 (MQ=255)
                                                                                         ccAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAGGTACGCCTGCTCGAg                                                                                 <  2:470302/130‑1 (MQ=255)
                                                                                         ccAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAGGTACGCCTGCTCGAGCGCCAGGGccac                                                                     <  2:2711215/142‑1 (MQ=255)
                                                                                         ccAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAGGTACGCCTGCTCGAGCGCCAGGGccac                                                                     >  1:2711215/1‑142 (MQ=255)
                                                                                         ccAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAGGTACGCCTGCTCGAGCGCCAGGGCCACCACGACGc                                                             >  1:1306673/1‑150 (MQ=255)
                                                                                              tGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAGGTACGCCTGCTCGAGCGCCAGGGCCACCACGACGCCTccc                                                        >  2:959077/1‑150 (MQ=255)
                                                                                                   cTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGcc                                                                                                            >  2:483994/1‑93 (MQ=255)
                                                                                                   cTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGcc                                                                                                            <  1:483994/93‑1 (MQ=255)
                                                                                                   cTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAGGTACGCCTGCTCGAGCGCCAGGGCCACCACGACGCCTCCCGCCAg                                                   >  1:894586/1‑150 (MQ=255)
                                                                                                           ccTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAGGTACGCCTGCTCGAGCGCCAGGGCCACCACGACGCCTCCCGCCAGGTGCTggg                                           >  2:356081/1‑150 (MQ=255)
                                                                                                           ccTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAGGTACGCCTGCTCGAGCGCCAGGGCCACCACGACGCCTCCCGCCAGGTGCTggg                                           <  2:689355/150‑1 (MQ=255)
                                                                                                            cTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCg                                                                                                                 <  1:1861130/79‑1 (MQ=255)
                                                                                                            cTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCg                                                                                                                 >  2:1861130/1‑79 (MQ=255)
                                                                                                            cTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAGGTACGCCTGCTCGAGCGCCAGGGCCACCACGACGCCTCCCGCCAGGTGCTGGGc                                          <  2:2377709/150‑1 (MQ=255)
                                                                                                               tACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAGGTACGCCTGCTCGAGCGCCAGGGCCACCACGACGCCTCCCGCCAGGTGCTGGGCaaa                                       >  1:1645482/1‑150 (MQ=255)
                                                                                                                   tGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGa                                                                                                                >  1:32222/1‑73 (MQ=255)
                                                                                                                   tGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAGGTACGCCTGCTCGAGCGCCAGGGCCACCACGACGCCTCCCGCCAGGTGCTGGGCAAAGccc                                   >  2:492775/1‑150 (MQ=255)
                                                                                                                   tGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAGGTACGCCTGCTCGAGCGCCAGGGCCACCACGACGCCTCCCGCCAGGTGCTGGGCAAAGccc                                   <  1:3029193/150‑1 (MQ=255)
                                                                                                                    gCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAGGTACGCCTGCTCGAGCGCCAgg                                                                          <  1:2563617/110‑1 (MQ=255)
                                                                                                                       cGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAGGTACGCCTGCTCGAGCGCCAGGGCCACCACGACGCCTCCCGCCAGGTGCTGGGCAAAGCCCTGgc                               >  1:2373067/1‑150 (MQ=255)
                                                                                                                          aaaCGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAGGTACGCCTGCTCGAGCGCCAGGGCCACCACGACGCCTCCCGCCAGGTGCTGGGCAAAGCCCTGGCGCt                            >  2:941561/1‑150 (MQ=255)
                                                                                                                            aCGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAGGTACGCCTGCTCGAGCGCCAGGGCCACCACGACGCCTCCCGCCAGGTGCTGGGCAAAGCCCTGGCGCTGa                          >  2:123857/1‑150 (MQ=255)
                                                                                                                            aCGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAGGTACGCCTGCTCGAGCGCCAGGGCCACCACGACGCCTCCCGCCAGGTGCTGGGCAAAGCCCTGGCGCTGa                          <  1:2804617/150‑1 (MQ=255)
                                                                                                                             cGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAGGTACGCCTGCTCGAGCGCCAGGGCCACCACGACGCCTCCCGCCAGGTGCTGGGCAAAGCCCTGGCGCTGAg                         <  2:1645482/150‑1 (MQ=255)
                                                                                                                              gACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAGGTACGCCTGCTCGAGCGCCAGGGCCACCACGACGCCTCCCGCCAGGTGCTGGGCAAAGCCCTGGCGCTGAGc                        >  1:2218411/1‑150 (MQ=255)
                                                                                                                                cTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGACCCCGGCAACCTCGATGCCCTGGCGACTCAGGTACGCCTGCTCGAGCGCCAGGGCCACCACGACGCCTCCCGCCAGGTGCTGGGCAAAGCCCTGGCGCTGAGccc                      <  2:2218411/150‑1 (MQ=255)
                                                                                                                                   cacgca‑cgCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAGGTACGCCTGCTCGAGCGCCAGGGCCACCACGACGCCTCCCGCCAGGTGCTGGGCAAAGCCCTGGCGCTGAGCCCCGa                   <  2:2310234/150‑1 (MQ=255)
                                                                                                                                    acgca‑cgcCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAGGTACGCCTGCTCGAGCGCCAGGGCCACCACGACGCCTCCCGCCAGGTGCTGGGCAAAGCCCTGGCGCTGAGCCCCGAc                  >  1:2299512/1‑150 (MQ=255)
                                                                                                                                      gca‑cgcCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAGGTACGCCTGCTCGAGCGCCAGGGc                                                                        <  1:1120044/94‑1 (MQ=255)
                                                                                                                                      gca‑cgcCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAGGTACGCCTGCTCGAGCGCCAGGGc                                                                        >  2:1120044/1‑94 (MQ=255)
                                                                                                                                      gca‑cgcCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAGGTACGCCTGCTCGAGCGCCAGGGCCACCACGACGCCTCCCGCCAGGTGCTGGGCAAAGCCCTGGCGCTGGGcccccgcac                >  2:880583/1‑145 (MQ=255)
                                                                                                                                      gca‑cgcCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAGGTACGCCTGCTCGAGCGCCAGGGCCACCACGACGCCTCCCGCCAGGTGCTGGGCAAAGCCCTGGCGCTGAGCCCCGACTc                >  1:1079487/1‑150 (MQ=255)
                                                                                                                                       ca‑cgcCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAGGTACGCCTGCTCGAGCGCCAGGGCCACCACGACGCCTCCCGCCAGGTGCTGGGCAAAGCCCTGGCGCTGAGCCCCGACTCg               <  1:100637/150‑1 (MQ=255)
                                                                                                                                       ca‑cgcCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAGGTACGCCTGCTCGAGCGCCAGGGCCACCACGACGCCTCCCGCCAGGTGCTGGGCAAAGCCCTGGCGCTGAGCCCCGACTCg               <  2:1079487/150‑1 (MQ=255)
                                                                                                                                        a‑cgcCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAGGTACGCCTGCTCGAGCGCCAGGGCCACCACGACGCCTCCCGCCAGGTGCTGGGCAAAGCCCTGGCGCTGAGCCCCGACTCgg              >  1:1521027/1‑150 (MQ=255)
                                                                                                                                           ctcgcTGCAGGGCTGCAGCG‑GCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAGGTACGCCTGCTCGAGCGCCAGGGCCACCACGACGCCTCCCGCCAGGTGCTGGGCAAAGCCCTGGCGCTGAGc                        <  2:620051/135‑1 (MQ=255)
                                                                                                                                           ctcgcTGCAGGGCTGCAGCG‑GCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAGGTACGCCTGCTCGAGCGCCAGGGCCACCACGACGCCTCCCGCCAGGTGCTGGGCAAAGCCCTGGCGCTGAGc                        >  1:620051/3‑137 (MQ=255)
                                                                                                                                            tcgcTGCAGGGCTGCAGCGGGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAGGTACGCCTGCTCGAGCGCCAGGGCCACCACGACGCCTCCCGCCAGGTGCTGGGCAAAGCCCTGGCGCTGAGCCCCGa                   >  2:3019236/2‑142 (MQ=255)
                                                                                                                                            tcgcTGCAGGGCTGCAGCGGGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAGGTACGCCTGCTCGAGCGCCAGGGCCACCACGACGCCTCCCGCCAGGTGCTGGGCAAAGCCCTGGCGCTGAGCCCCGa                   <  1:3019236/141‑1 (MQ=255)
                                                                                                                                              gcTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAGGTACGCCTGCTCGAGCGCCAGGGCCACCACGACGCCTCCCGCCAGGTGCTGGGCAAAGCCCTGGCGCTGAGCCCCGACTCGGCCttt         <  2:2860709/150‑1 (MQ=255)
                                                                                                                                                tGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAGGTACGCCTGCTCGAGCGCCAGGGCCACCACGACGCCTCCCGCCAGGTGCTGGGc                                          <  1:923299/115‑1 (MQ=255)
                                                                                                                                                tGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAGGTACGCCTGCTCGAGCGCCAGGGCCACCACGACGCCTCCCGCCAGGTGCTGGGc                                          >  2:923299/1‑115 (MQ=255)
                                                                                                                                                tGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAGGTACGCCTGCTCGAGCGCCAGGGCCACCACGACGCCTCCCGCCAGGTGCTGGGCAAAGCCCTGGCGCTGAGCCCCGACTCGGCCTTTCt       <  1:941561/150‑1 (MQ=255)
                                                                                                                                                   aGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGAt                                                                                                               >  1:1388354/1‑43 (MQ=255)
                                                                                                                                                   aGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGAt                                                                                                               <  2:1388354/43‑1 (MQ=255)
                                                                                                                                                   aGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAGGTACGCCTGCTCGAGCGCCAGGGCCACCACGACGCCTCCCGCCAGGTGCTGGGCAAAGCCCTGGCGCTGAGCCCCGACTCGGCCTTTCTGCa    <  1:1309937/150‑1 (MQ=255)
                                                                                                                                                     cccTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAGGTACGCCTGCTCGAGCGCCAGGGCCACCACGACGCCTCCCGCCAGGTGCTGGGCAAAGCCCTGGCGCTGAGCCCCGACTCGGCCTTTCTGCAGt  >  1:1186996/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GACGCCATCGGCCTCTATTTCGGCCCCCTGCAGTCAGCCCTGGAGCAATACCAGCAAGCCCTGGAGCAACAGCCTGAAGATGCCGACGCCCAAGTGCACCTGGCGCGCCTGTACCTGCACGAAAACGACTACACGCA‑CGCCGCTGCAGCCCTGCAGCGAGCGCTCGCGGTTGCCCCCGGCAACCTCGATGCCCTGGCGACTCAGGTACGCCTGCTCGAGCGCCAGGGCCACCACGACGCCTCCCGCCAGGTGCTGGGCAAAGCCCTGGCGCTGAGCCCCGACTCGGCCTTTCTGCAGT  >  NC_002947/3883467‑3883764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑