Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,338,101 C→G 6.3% intergenic (+60/‑224) PP_4696 → / → PP_mr53 nitrogen regulation protein NR(I)/CrcZ ncRNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,338,1010CG 6.3% 285.8 / 7.9 91intergenic (+60/‑224)PP_4696/PP_mr53nitrogen regulation protein NR(I)/CrcZ ncRNA
Reads supporting (aligned to +/- strand):  ref base C (37/47);  new base G (2/4);  total (39/52)
Fisher's exact test for biased strand distribution p-value = 6.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

GCTCGGCGTAAGCCGCAAGTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGACATTTTGAACCTGC  >  NC_002947/5337957‑5338247
                                                                                                                                                |                                                                                                                                                   
gCTCGGCGTAAGCCGCAAGTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCg‑cggc                                                                                                                                               <  2:470267/150‑1 (MQ=255)
    ggCGTAAGCCGCAAGTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTc                                                                                                                                           >  1:3000929/1‑150 (MQ=255)
      cGTAAGCCGCAAGTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGGCCGAGCGCCGCGCGGG‑CGGCGCTc                                                                                                                                           >  1:1997794/1‑148 (MQ=255)
      cGTAAGCCGCAAGTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGGCCGAGCGCCGCGCGGG‑CGGCGCTc                                                                                                                                           <  2:1997794/148‑1 (MQ=255)
           gCCGCAAGTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTca                                                                                                                                    >  1:1683497/1‑150 (MQ=255)
            ccGCAAGTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCCCCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGGCCGAGCGCCGCGCGGG‑CGGCGCTCGGTCTcac                                                                                                                                   <  1:2985196/150‑1 (MQ=39)
            ccGCAAGTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTcac                                                                                                                                   >  1:2935853/1‑150 (MQ=255)
              gCAAGTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAg                                                                                                                                 <  2:2935853/150‑1 (MQ=255)
               cAAGTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAgg                                                                                                                                <  1:831138/150‑1 (MQ=255)
                 aGTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGgcg                                                                                                                              <  2:1045980/150‑1 (MQ=255)
                  gTGCCTGTGGCAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAACAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGgcgc                                                                                                                             <  1:1457428/150‑1 (MQ=255)
                       tgtgtgAACTCCGCCATCGCCTGTGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCGCCTACGCACCGTACTCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGTTCGGCCTCACAGGCGCTGAAc                                                                                                                        <  2:379521/150‑1 (MQ=38)
                        gtgGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACg                                                                                                                       <  1:2505678/150‑1 (MQ=255)
                         tgGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACgt                                                                                                                      <  2:2025215/150‑1 (MQ=255)
                           ggAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACgtgt                                                                                                                    >  2:286261/1‑150 (MQ=255)
                           ggAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACgtgt                                                                                                                    <  1:1028577/150‑1 (MQ=255)
                           ggAACGCCGCCAGCCCCGGGGCATCCGACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTTAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACgtgt                                                                                                                    <  2:102522/150‑1 (MQ=255)
                                gccgccAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCacga                                                                                                               <  2:1511713/150‑1 (MQ=255)
                                gccgccAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCacga                                                                                                               >  1:1014594/1‑150 (MQ=255)
                                 ccgccAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCacgac                                                                                                              >  1:248285/1‑150 (MQ=255)
                                  cgccAGCGCCTGGGCATCCCACGGCGAAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCACCCTATGCGCCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCacgacg                                                                                                             >  1:2861588/1‑150 (MQ=255)
                                    ccAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGaa                                                                                                           <  1:286261/150‑1 (MQ=255)
                                       gcgcCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCGCGGG‑CGGCGCTCg                                                                                                                                          >  1:770793/1‑116 (MQ=255)
                                       gcgcCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCGCGGG‑CGGCGCTCg                                                                                                                                          <  2:770793/116‑1 (MQ=255)
                                           cTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGTCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCa                                                                                                    <  2:2281540/150‑1 (MQ=255)
                                           cTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCa                                                                                                    >  1:3044746/1‑150 (MQ=255)
                                           cTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCa                                                                                                    <  2:2796757/150‑1 (MQ=255)
                                           cTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCa                                                                                                    >  1:1326512/1‑150 (MQ=255)
                                           cTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCGACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCTTCACAGGCGCTGAACGTGTCACGACGAACACCCCa                                                                                                    <  1:2255867/150‑1 (MQ=255)
                                            tGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGGCGGTGAGACCGAGCACCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAg                                                                                                   <  2:986193/150‑1 (MQ=255)
                                                cATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCcgcg                                                                                                                                                    >  1:257720/1‑98 (MQ=255)
                                                cATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCcgcg                                                                                                                                                    <  2:257720/98‑1 (MQ=255)
                                                  tCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAaca                                                                                             >  2:1084626/1‑150 (MQ=255)
                                                   cccACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGCGACCTAGCGCCTACCAAGACGGCG‑TTGGCGGCGCATTCGTTTAACGTGTCACGACGAACACCCCAGGTAacac                                                                                            <  1:59454/150‑1 (MQ=255)
                                                   cccACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAacac                                                                                            <  2:787008/150‑1 (MQ=255)
                                                      aCGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTcaca                                                                                                                                  >  1:1523510/1‑109 (MQ=255)
                                                      aCGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTcaca                                                                                                                                  <  2:1523510/109‑1 (MQ=255)
                                                      aCGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAAcacctc                                                                                         >  2:2559922/1‑150 (MQ=255)
                                                      aCGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAAcacctc                                                                                         <  1:1907119/150‑1 (MQ=255)
                                                        ggCGCAAGAGCAACGCTACCAGCGAATACACCCCCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGTGCCCCCGGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACTACCAATACCCCTGGTAAcacctcac                                                                                       <  2:811414/150‑1 (MQ=255)
                                                           gcAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCacga                                                                                                               <  2:222066/123‑1 (MQ=255)
                                                           gcAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCacga                                                                                                               >  1:222066/1‑123 (MQ=255)
                                                             aaGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTccc                                                                                  <  1:3010466/150‑1 (MQ=255)
                                                              agagCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCg                                                                                 >  2:2090275/1‑150 (MQ=255)
                                                              agagCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCg                                                                                 >  2:1214968/1‑150 (MQ=255)
                                                                   aaCGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTaaaa                                                                            >  2:3048249/1‑150 (MQ=255)
                                                                     cGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAAt                                                                          >  1:1848532/1‑150 (MQ=255)
                                                                       cTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTccc                                                                                  >  2:789422/1‑140 (MQ=255)
                                                                       cTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTccc                                                                                  <  1:789422/140‑1 (MQ=255)
                                                                       cTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTCTACGGTTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCt                                                                        <  1:2090275/150‑1 (MQ=255)
                                                                          ccAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGtt                                                                     <  2:1683497/150‑1 (MQ=255)
                                                                           cAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTaa                                                                                               >  1:2251965/1‑123 (MQ=255)
                                                                           cAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTaa                                                                                               <  2:2251965/123‑1 (MQ=255)
                                                                           cAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTa                                                                    <  2:1014594/150‑1 (MQ=255)
                                                                            aGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTAc                                                                   <  2:169432/150‑1 (MQ=255)
                                                                               gAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACCACGAAAACACTATAAAACATTTCATCTCCCGGAAAAATCTGTTAcaca                                                                >  2:2336139/1‑150 (MQ=37)
                                                                                aaTAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCaa                                                               <  2:2494036/150‑1 (MQ=255)
                                                                                 aTAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAg                                                              <  1:1496530/150‑1 (MQ=255)
                                                                                  tAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCCCTTGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGc                                                             <  1:2336139/150‑1 (MQ=255)
                                                                                   aaCCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCt                                                            >  2:1008194/1‑150 (MQ=255)
                                                                                    aCCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCa                                                                                                    <  1:1884924/109‑1 (MQ=255)
                                                                                    aCCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCCCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCa                                                                                                    >  2:1884924/1‑109 (MQ=255)
                                                                                          cTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACAcccc                                                                                                     >  2:1420288/1‑102 (MQ=255)
                                                                                          cTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACAcccc                                                                                                     <  1:1420288/102‑1 (MQ=255)
                                                                                            aCGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAGCACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGt                                                   >  1:2318526/1‑150 (MQ=255)
                                                                                            aCGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGt                                                   <  2:248285/150‑1 (MQ=255)
                                                                                              gcaccgcacGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTaa                                                 <  1:1773878/150‑1 (MQ=255)
                                                                                               caccgcacGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAAc                                                <  2:3000929/150‑1 (MQ=255)
                                                                                                 ccgcacGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAg                                              >  1:1961222/1‑150 (MQ=255)
                                                                                                 ccgcacGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAg                                              >  2:2722623/1‑150 (MQ=255)
                                                                                                  cgcacGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTa                                                                    >  2:424417/1‑127 (MQ=255)
                                                                                                  cgcacGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTa                                                                    <  1:424417/127‑1 (MQ=255)
                                                                                                  cgcacGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGt                                             <  1:1084626/150‑1 (MQ=255)
                                                                                                   gcacGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAac                                                                                              >  2:870612/1‑100 (MQ=255)
                                                                                                   gcacGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAac                                                                                              <  1:870612/100‑1 (MQ=255)
                                                                                                    cacGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCt                                                                        <  1:374103/121‑1 (MQ=255)
                                                                                                    cacGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCt                                                                        >  2:374103/1‑121 (MQ=255)
                                                                                                    cacGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTg                                                                       >  1:2784623/1‑122 (MQ=255)
                                                                                                    cacGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTg                                                                       <  2:2784623/122‑1 (MQ=255)
                                                                                                         cgcAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAg                                      <  1:1214968/150‑1 (MQ=255)
                                                                                                              ccTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTa                                 >  1:1390119/1‑150 (MQ=255)
                                                                                                                tATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTAc                                                                   <  2:20082/114‑1 (MQ=255)
                                                                                                                tATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTAc                                                                   >  1:20082/1‑114 (MQ=255)
                                                                                                                           ggTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAAtt                    >  1:238076/1‑150 (MQ=255)
                                                                                                                              gagaCCGCGCGCCGCCCCCC‑CGGCGCTCGGCCACACAGGCGCTGAACGTGTGAGGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCg                 <  2:2861588/150‑1 (MQ=255)
                                                                                                                               agaCCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGa                <  2:238076/150‑1 (MQ=255)
                                                                                                                                  ccGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGACAt             >  2:2158977/1‑150 (MQ=255)
                                                                                                                                   cGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGt                                                                                                 <  2:2284843/65‑1 (MQ=255)
                                                                                                                                   cGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGt                                                                                                 >  1:2284843/1‑65 (MQ=255)
                                                                                                                                   cGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAAcacct                                                                                          >  1:469230/1‑72 (MQ=255)
                                                                                                                                   cGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAAcacct                                                                                          <  2:469230/72‑1 (MQ=255)
                                                                                                                                   cGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGACAtt            >  1:2620982/1‑150 (MQ=255)
                                                                                                                                     ggcgcCGCCCGGG‑CGGCGCTCGGCCTCACAAGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCGCCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGACAtttt          <  2:1326512/149‑1 (MQ=255)
                                                                                                                                       gtgaggccgaG‑CGCCGCGCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGACATTTTGa        <  2:235879/144‑1 (MQ=255)
                                                                                                                                         ccgcccgcG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAGCACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGACATTTTGAAc      <  2:2318526/150‑1 (MQ=255)
                                                                                                                                           gcccgcG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCa                                                                                                    >  2:1810115/1‑54 (MQ=255)
                                                                                                                                           gcccgcG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCa                                                                                                    <  1:1810115/54‑1 (MQ=255)
                                                                                                                                           gcccgcG‑CGGCGCTCGGCCTCACAGGCGCTGAACGCGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGACATTTTGAACCt    >  2:52716/1‑150 (MQ=255)
                                                                                                                                             ccgcG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGACATTTTGAACCTGc  <  1:2559922/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
GCTCGGCGTAAGCCGCAAGTGCCTGTGGGAACGCCGCCAGCGCCTGGGCATCCCACGGCGCAAGAGCAACGCTACCAGCGAATAACCCACCTACGCACCGCACGCCGCAGCCTATGCGGCGTCGGTGAGACCGAGCGCCGCCCGCG‑CGGCGCTCGGCCTCACAGGCGCTGAACGTGTCACGACGAACACCCCAGGTAACACCTCACCTCCCGTAAAAATCTGTTACCCAAGCTTTCCGCCGTAACAGTCCCCGAGGCGTACGGTAACGAAATTGCGACATTTTGAACCTGC  >  NC_002947/5337957‑5338247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑