BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,384,576	G→C	5.1%	G331A (GGC→GCC)	lldP →	L‑lactate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,384,576	0	G	C	5.1%	349.6 / 7.4	111	G331A (GGC→GCC)	lldP	L‑lactate permease
Reads supporting (aligned to +/- strand): ref base G (51/54); new base C (4/2); total (55/56)
Fisher's exact test for biased strand distribution p-value = 4.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

Only 100 of 120 total aligned reads displayed.

TGTCGGCGCAGCCGTTGTGCAGCCAAGCGGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCG‑CCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGACCCGATCTCCGCCACCGGCACCGCGATCTTC  >  NC_002947/5384427‑5384724

tgtCGGCGCAGCCGTTGTGCAGCCAAGCGGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCG‑CCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCggg                                                                                                                                                      >  2:391741/1‑150 (MQ=255)

 gtCGGCGCAGCCGTTGTGCAGCCAAGCGGCAGCCAGCCTAGCCCGTACCGCGTTGGCGAGATCTTCAAGGCCTGGTCG‑CCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGggc                                                                                                                                                     >  1:1616240/1‑150 (MQ=255)

 gtCGGCGCAGCCGTTGTGCAGCCAAGCGGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCG‑CCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGggc                                                                                                                                                     <  1:2244971/150‑1 (MQ=255)

    ggCGCAGCCGTTGTGCAGCCAAGCGGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCG‑CCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGggcggc                                                                                                                                                  >  2:797922/1‑150 (MQ=255)

     gcgcAGCCGTTGTGCAGCCAAGCGGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCG‑CCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGgcggcg                                                                                                                                                 >  2:2140082/1‑150 (MQ=255)

       gcAGCCGTTGTGCAGCCAAGCGGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCG‑CCGTTCCTGATCCTCACCGGGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGcgcg                                                                                                                                               <  1:1280529/150‑1 (MQ=255)

         aGCCGTTGTGCTGCCAAGCTGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGATCTT‑AGGGGAAAGACGTTCGGTCCCGATCCTCACCTTGCTGGTAACCCTCTGGCCCCTCAAGCGGTTTAATGCGGCTTTCGCGCCGGGCGGCGCGAt                                                                                                                                             <  1:700656/150‑1 (MQ=255)

         aGCCGTTGTGCAGCCAAGCGGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCG‑CCGTTCCTGATCCTCCCCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGAt                                                                                                                                             <  2:1394136/150‑1 (MQ=255)

            cGTTGTGCAGCCAAGCGGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCG‑CCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTa                                                                                                                                          >  1:2468109/1‑150 (MQ=255)

                   cAGCCAAGCGGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCG‑CCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTc                                                                                                                                   <  1:109021/150‑1 (MQ=255)

                   cAGCCAAGCGGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCG‑CCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACGTc                                                                                                                                   <  1:121890/150‑1 (MQ=255)

                   cAGCCAAGCGGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCG‑CCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACCTc                                                                                                                                   <  2:2295495/150‑1 (MQ=255)

                      ccAAGCGGCAGCCAGCCTAGCCCGTACAGTTTTGGCGAGATCTTCAAGGCCTGGTCG‑CCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCgtg                                                                                                                                <  2:387403/150‑1 (MQ=255)

                      ccAAGCGGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCG‑CCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCgtg                                                                                                                                <  2:603369/150‑1 (MQ=255)

                      ccAAGCGGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCG‑CCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCgtg                                                                                                                                <  1:797922/150‑1 (MQ=255)

                      ccAAGCGGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCG‑CCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCgtg                                                                                                                                >  1:798876/1‑150 (MQ=255)

                      ccAAGCGGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCG‑CCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCgt                                                                                                                                 <  1:1387404/149‑1 (MQ=255)

                      ccAAGCGGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCG‑CCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCgt                                                                                                                                 >  2:1387404/1‑149 (MQ=255)

                      ccAAGCGGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCG‑CCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGCCCGGCGCGa                                                                                                                                              <  1:2049897/136‑1 (MQ=255)

                      ccAAGCGGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCG‑CCGTTACTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCgtg                                                                                                                                >  1:1351435/1‑150 (MQ=255)

                       cAAGCGGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCG‑CCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCgtgt                                                                                                                               <  2:376882/150‑1 (MQ=255)

                         aGCGGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCG‑CCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTc                                                                                                                             <  1:2193534/150‑1 (MQ=255)

                               agccagccTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCG‑CCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTc                                                                                                                       <  1:2445177/150‑1 (MQ=255)

                                       tAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCG‑CCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATccc                                                                                                               >  1:2739027/1‑150 (MQ=255)

                                       tAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCG‑CCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCAGAGCCGCGCGAGGTGCTTCTACGTTTTCAACTTCGCCATccc                                                                                                               >  2:2334821/1‑150 (MQ=255)

                                        aGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCG‑CCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCg                                                                                                              <  1:1666947/150‑1 (MQ=255)

                                         gCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCG‑CCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGc                                                                                                             >  1:2951650/1‑150 (MQ=255)

                                             gTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCG‑CCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCt                                                                                                         >  2:2589611/1‑150 (MQ=255)

                                              tACAGCTTTGGCGAGATCTTCAAGGCCTGGTCG‑CCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTc                                                                                                        <  2:1405717/150‑1 (MQ=255)

                                                 aGCTTTGGCGAGATCTTCAAGGCCTGGTCG‑CCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGAc                                                                                                     <  1:2140082/150‑1 (MQ=255)

                                                   cTTTGGCGAGATCTTCAAGGCCTGGTCG‑CCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCa                                                                                                   >  2:2729763/1‑150 (MQ=255)

                                                     ttGGCGAGATCTTCAAGGCCTGGTCG‑CCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGGGCCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGc                                                                                                 <  1:2996580/150‑1 (MQ=255)

                                                       ggCGAGATCTTCAAGGCCTGGTCG‑CCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTg                                                                                               <  2:17614/150‑1 (MQ=255)

                                                       ggCGAGATCTTCAAGGCCTGGTCG‑CCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTg                                                                                               <  2:2739027/150‑1 (MQ=255)

                                                          gagaTCTTCAAGGCCTGGTCG‑CCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGCGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTg                                                                                            >  2:1679572/1‑150 (MQ=255)

                                                           agaTCTTCAAGGCCTGGTCG‑CCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGa                                                                                           <  1:2450732/150‑1 (MQ=255)

                                                            gaTCTTCAAGGCCTGGTCG‑CCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGAt                                                                                          <  2:2951650/150‑1 (MQ=255)

                                                            gaTCTTCAAGGCCTGGTCG‑CCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGAt                                                                                          <  2:71195/150‑1 (MQ=255)

                                                             aTCTTCAAGGCCTGGTCG‑CCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATc                                                                                         <  2:2850198/150‑1 (MQ=255)

                                                                ttCAAGGCCTGGTCG‑CCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGAACCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAg                                                                                      >  1:1520826/1‑150 (MQ=255)

                                                                 tCAAGGCCTGGTCG‑CCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGa                                                                                     >  2:2433255/1‑150 (MQ=255)

                                                                    aGGCCTGGTCG‑CCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCTTGTTCAACTTCCCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCg                                                                                  >  1:1743503/1‑150 (MQ=255)

                                                                    aGGCCTGGTCG‑CCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCg                                                                                  >  2:1311663/1‑150 (MQ=255)

                                                                      gcgtGGTCG‑CCGTTCCTGTCCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTGCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCa                                                                                <  2:951069/147‑1 (MQ=255)

                                                                      gCCTGGTCG‑CCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTc                                                                                                                                   <  2:2516283/99‑1 (MQ=255)

                                                                      gCCTGGTCG‑CCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCa                                                                                <  2:798876/150‑1 (MQ=255)

                                                                                ccGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGtt                                                                       <  2:2468109/150‑1 (MQ=255)

                                                                                ccGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGtt                                                                       <  2:2386534/150‑1 (MQ=255)

                                                                                  gTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGc                                                                     <  1:86561/150‑1 (MQ=255)

                                                                                    tCCTGATCCTCACCGTGCTGGTCACCATCTGGACTCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCcg                                                                   <  1:1095251/150‑1 (MQ=255)

                                                                                       tGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCa                                                                                >  1:561491/1‑134 (MQ=255)

                                                                                       tGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCa                                                                                <  2:561491/134‑1 (MQ=255)

                                                                                           ccTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGAc                                                            <  1:3051403/150‑1 (MQ=255)

                                                                                           ccTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGAc                                                            >  2:1504735/1‑150 (MQ=255)

                                                                                            cTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAGGGCGGCTTTCGCGCCggg                                                                                                                                                      >  1:621255/1‑59 (MQ=255)

                                                                                            cTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAGGGCGGCTTTCGCGCCggg                                                                                                                                                      <  2:621255/59‑1 (MQ=255)

                                                                                             tCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGCCGGGGCGATTTACACATTCGTGTTCAACTTCGCCATCCCGCACCTCGACCCGCTGTTAGTCAAGACCGCACCGATCATTGCAGCGGCGACCg                                                          >  2:566910/1‑150 (MQ=255)

                                                                                              cACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGtt                                                                       <  2:1404287/136‑1 (MQ=255)

                                                                                              cACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGtt                                                                       >  1:1404287/1‑136 (MQ=255)

                                                                                              cACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTTCCGCGCCGACCGc                                                         >  2:1766240/1‑150 (MQ=255)

                                                                                               aCCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATcc                                                                                                                <  1:2625074/94‑1 (MQ=255)

                                                                                                 cGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCAt                                                      <  2:852810/150‑1 (MQ=255)

                                                                                                    gCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGcc                                                   <  2:2214098/150‑1 (MQ=255)

                                                                                                         tCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGATCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCcggcgg                                              <  1:2334821/150‑1 (MQ=255)

                                                                                                           aCCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATc                                                                          >  2:416205/1‑120 (MQ=255)

                                                                                                           aCCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGgtg                                            >  1:2438200/1‑150 (MQ=255)

                                                                                                           aCCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGgtg                                            >  1:1075377/1‑150 (MQ=255)

                                                                                                            ccATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGgtgt                                           <  1:2589611/150‑1 (MQ=255)

                                                                                                             cATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGtt                                          >  1:1317348/1‑150 (MQ=255)

                                                                                                              aTCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTTCCGCGCCGACCGCCCTGCCGGCGGTGTTc                                         >  2:1286850/1‑150 (MQ=255)

                                                                                                                cTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCaa                                       <  2:2180962/150‑1 (MQ=255)

                                                                                                                    aCCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTc                                   >  2:23141/1‑150 (MQ=255)

                                                                                                                     cccTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCg                                  >  1:2252981/1‑150 (MQ=255)

                                                                                                                        tGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGAcc                               >  1:1973871/1‑150 (MQ=255)

                                                                                                                         gAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGAccc                              >  1:1080177/1‑150 (MQ=255)

                                                                                                                         gAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGAccc                              >  1:2855273/1‑150 (MQ=255)

                                                                                                                             ccGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGACCCGAtc                          <  2:1075377/150‑1 (MQ=255)

                                                                                                                               gTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGACCCGAtctc                        >  1:2732278/1‑150 (MQ=255)

                                                                                                                               gTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGACCCGAtctc                        >  2:1931392/1‑150 (MQ=255)

                                                                                                                                     ggcggcTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGAc                                                                                    >  1:1832837/1‑84 (MQ=255)

                                                                                                                                     ggcggcTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGAc                                                                                    <  2:1832837/84‑1 (MQ=255)

                                                                                                                                     ggcggcTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGACCCGATCTCCGCCAc                  >  1:611843/1‑150 (MQ=255)

                                                                                                                                     ggcggcTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGACCCGATCTCCGCCAc                  >  1:902991/1‑150 (MQ=255)

                                                                                                                                      gcggcTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGGCCCTCGACCAACTGGTGATCAAGACCGCACCGATCGTTTCCCCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGACCCGATCTCCGCCAcc                 >  1:344930/1‑150 (MQ=255)

                                                                                                                                      gcggcTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGc                                     <  2:2250621/130‑1 (MQ=255)

                                                                                                                                      gcggcTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGc                                     >  1:2250621/1‑130 (MQ=255)

                                                                                                                                       cggcTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGACCCGATCTCCGCCACCg                >  2:1208238/1‑150 (MQ=255)

                                                                                                                                        gtacatCGCGCCGCCCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGc                                     <  1:959971/123‑1 (MQ=255)

                                                                                                                                         gcTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGACCCGATCTCCGCCACCGGc              >  1:1310740/1‑150 (MQ=255)

                                                                                                                                          cTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGACCCGATCTCCGCCACCGGCa             <  2:2160070/150‑1 (MQ=255)

                                                                                                                                          cTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGACACAAACCGCACCGATCGTTGCCGCGCCGAGCGCTGAGCCTTCGGGTTTCAAGCTCGACCCGATCTCCGCCACCGGCa             >  1:1454136/1‑150 (MQ=255)

                                                                                                                                           tttCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGAcc                                                           <  1:2429896/103‑1 (MQ=255)

                                                                                                                                           tttCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGAcc                                                           >  2:2429896/1‑103 (MQ=255)

                                                                                                                                           tttCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGACCCGATCTCCGCCACCGGCAc            >  2:2645011/1‑150 (MQ=255)

                                                                                                                                           catCGCGCCGCCCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGACCCGATCTCCGCCACCGGCAc            >  1:2594492/3‑150 (MQ=255)

                                                                                                                                             tCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCTGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGACCCGATCTCCGCCACCGGCACcg          <  2:2053028/150‑1 (MQ=255)

                                                                                                                                                 gcCGGGCGGCGCGCTGTACAACCTCGTGTTTAACGTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGACCCGATCTCCGCCACCGGCACCGCGAt      <  2:803753/150‑1 (MQ=255)

                                                                                                                                                 gcCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGACCCGATCTCCGCCACCGGCACCGCGAt      <  2:1317348/150‑1 (MQ=255)

                                                                                                                                                 gcCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTAGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGAGCGCCATGCCGGTGGTGTTCAAGGCCGACGCGATCTCCGCCACCGGCACCGCGAt      >  2:335051/1‑150 (MQ=255)

                                                                                                                                                     ggcggcTCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGACCCGATCTCCGCCACCGGCACCGCGATCTTc  <  1:786651/150‑1 (MQ=255)

TGTCGGCGCAGCCGTTGTGCAGCCAAGCGGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCG‑CCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGACCCGATCTCCGCCACCGGCACCGCGATCTTC  >  NC_002947/5384427‑5384724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`