BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,577,398	C→G	5.0%	L357V (CTG→GTG)	PP_4906 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,577,398	0	C	G	5.0%	415.0 / 9.9	130	L357V (CTG→GTG)	PP_4906	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (61/62); new base G (4/3); total (65/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01

Only 100 of 138 total aligned reads displayed.

CCCGCCGCCACGCCCGGCACGCCCTGTTCCACCCGGCCGAGGCCTTGATCTGGCCGTGGCACCAACGCCGCCCTCACCCGGACATGCTGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGCCCTGCTGGCCTGCGGCATGCAACGCATCATGCTGCTCAGCCTGGACAAGGCCAGCGACTACCTGCGCGTGCAGCAAATTGCCGGCCTG  >  NC_002947/5577252‑5577547

cccgccGCCACGCCCGGCACGCCCTGTTCCACCCGGCCGAGGCCTTGATCTGGCCGTGGCACCAACGCCGCCCTCACCCGGACATGCTGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACctgc                                                                                                                                                     <  2:2738053/150‑1 (MQ=255)

 ccgccgCCACGCCCGGCACGCCCTGTTCCACCCGGCCGAGGCCTTGATCTGGCCGTGGCACCAACGCCGCCCTCACCCGGACATGCTGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACctgct                                                                                                                                                    >  1:1843231/1‑150 (MQ=255)

 ccgccgCCACGCCCGGCACGCCCTGTTCCACCCGGCCGAGGCCTTGATCTGGCCGTGGCACCAACGCCGCCCTCACCCGGACATGCTGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACctgcc                                                                                                                                                    >  2:930232/1‑149 (MQ=255)

        cACGCCCGGCACGCCCTGTTCCACCCGGCCGAGGCCTTGATCTGGCCGTGGCACCAACGCCGCCCTCACCCGGACATGCTGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGc                                                                                                                                                 <  2:2400400/146‑1 (MQ=255)

         aCGCCCGGCACGCCCTGTTCCACCCGGCCGAGGCCTTGATCTGGCCGTGGCACCAACGCCGCCCTCACCCGGACATGCTGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAAc                                                                                                                                            >  2:112370/1‑150 (MQ=255)

         aCGCCCGGCACGCCCTGTTCCACCCGGCCGAGGCCTTGATCTGGCCGTGGCACCAACGCCGCCCTCACCCGGACATGCTGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAAc                                                                                                                                            <  1:2800777/150‑1 (MQ=255)

           gCCCGGCACGCCCTGTCCCACCCGGCCGAGGCCTTGATCTGGCCGTGGCACCAACGCCGCCCTCACCCGGACATGCTGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAAccc                                                                                                                                          <  2:1074204/150‑1 (MQ=255)

                     cccTGTTCCACCCGGCCGAGGCCTTGATCTGGCCGTGGCACCAACGCCGCCCTCACCCGGACATGCTGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCa                                                                                                                                <  2:605223/150‑1 (MQ=255)

                     cccTGTTCCACCCGGCCGAGGCCTTGATATGGCCGTGGCACCAACGCCGCCCTCACCCGGACATGCTGGCCCCGCCTCAGCACAGCAGCGACGTTCTTGATAGCTCGGGCATTACGTGCGATGACCTGCTGGCCCAACCCAGCCCGTTCa                                                                                                                                <  1:1303872/150‑1 (MQ=255)

                      ccTGTTCCACCCGGCCGAGGCCTTGATCTGGCCGTGGCACCAACGCCGCCCTCGCCCGGACATGCTGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCAc                                                                                                                               >  2:35001/1‑150 (MQ=255)

                        tGTTCCACCCGGCCGAGGCCTTGATCTGGCCGTGGCACCAACGCCGCCCTCACCCGGACATGCTGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGcac                                                                                                                                               >  1:1076856/1‑130 (MQ=255)

                        tGTTCCACCCGGCCGAGGCCTTGATCTGGCCGTGGCACCAACGCCGCCCTCACCCGGACATGCTGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGcac                                                                                                                                               <  2:1076856/132‑3 (MQ=255)

                                ccGGCCGAGGCCTTGATCTGGCCGTGGCACCAACGCCGCCCTCACCCGGACATGCTGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTg                                                                                                                     <  2:2928726/150‑1 (MQ=255)

                                ccGGCCGAGGCCTTGATCTGGCCGTGGCACCAACGCCGCCCTCACCCGGACATGCTGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTg                                                                                                                     >  1:26363/1‑150 (MQ=255)

                                 cGGCCGAGGCCTTGATCTGGCCGTGGCACCAACGCCGCCCTCACCCGGACATGCTGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGc                                                                                                                    <  1:3039452/150‑1 (MQ=255)

                                      gAGGCCTTGATCTGGCCGTGGCACCAACGCCGCCCTCACCCGGACATGCTGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCTTTCACCAACGCCGTGCACCT‑g                                                                                                              <  2:508305/150‑1 (MQ=255)

                                      gAGGCCTTGATCTGGCCGTGGCACCAACGCCGCCCTCACCCGGACATGCTGGCCCCGCCACCGCCCAGCACCGACGACCTCGCACGCTGGCCCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCCACGCCGTCCACCT‑g                                                                                                              >  2:1256294/1‑150 (MQ=255)

                                           cTTGATCTGGCCGTGGCACCAACGCCGCCCTCACCCGGACATGCTGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCAc                                                                                                         >  2:3071453/1‑150 (MQ=255)

                                           cTTGATCTGGCCGTGGCACCAACGCCGCCATCACCCGGAAATGCTGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCAc                                                                                                         >  1:1005654/1‑150 (MQ=255)

                                              tctCTGGGCGTGGCACCCACGCCGCGCCCACCCGCACATCCTGGATGCCACACCGCCCAGCCGGGTCGACCTGACACGCGGGCGCACATTCTGGCAGCACCTGCTGGCCCAACCCGGCCCGGTCACCAACGCCGTGCACCT‑GGCCACCCa                                                                                                      <  2:2363161/148‑1 (MQ=255)

                                                tCTTGCCGTGGCACCAACGCCGCCCTCACCCGGACATGCTGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAgg                                                                                                    <  1:1286175/150‑1 (MQ=255)

                                                  tGGCCGTGGCACCAACGCCGCCCTCCCCCGGCCATACTGGTAGCAACAACGTACCCCAGCGCCGGCCGGGCTCGCTGGCGCACATTGTGCCAGCACCTGCTGTCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAgaga                                                                                                  >  1:2363161/1‑147 (MQ=255)

                                                   ggCCGTGGCACCAACGCGGACCTCAGCCGGACATGCTGGCGCCGCCACCGCCCAGCAGCGATGACCTGGCACCCTGGCGCCCATTGTGCCAGCACCTGCTGGCCCCACCCAGCCCGTTCACCAACGCCGTGCAGCT‑GGCCACCCAGGccc                                                                                                 >  2:1540633/1‑150 (MQ=255)

                                                    gCCGTGGCACCAACGCCGCCCTCACCCGGACATGCTGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCa                                                                                                >  1:422567/1‑150 (MQ=255)

                                                    gCCGTGGCACCAACGCCGCCCTCACCCGGACATGCTGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGGGCCAGCAGGTGCTGGCACaat                                                                                                                                            <  1:1888388/107‑2 (MQ=255)

                                                    gCCGTGGCACCAACGCCGCCCTCACCCGGACATGCTGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGGGCCAGCAGGTGCTGGCACaat                                                                                                                                            >  2:1888388/1‑106 (MQ=255)

                                                     ccGTGGCACCAACGCCGCCCTCACCCGGACATGCTGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCaa                                                                                               <  1:3035310/150‑1 (MQ=255)

                                                      cGTGGCACCAACGCCGCCCTCACCCGGACATGCTGGCCCCGTCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCaag                                                                                              <  2:2097636/150‑1 (MQ=255)

                                                        tGGCACCAACGCCGCCCTCACCCGGACATGCTGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCaagaa                                                                                            <  2:2926670/150‑1 (MQ=255)

                                                         ggCACCAGCGCCGCCCTCCCCCGGACATGCTGGGCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGACCAACCCCGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCaagaag                                                                                           <  1:1256294/150‑1 (MQ=255)

                                                           cACCAACGCCGCCCTCACCCGGACATGCTGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGcc                                                                                         <  1:1161605/150‑1 (MQ=255)

                                                           cACCAACGCCGCCCTCACCCGGACATGCTGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGcc                                                                                         <  1:1729973/150‑1 (MQ=255)

                                                            aCCAACGCCGCCCTCACCCGGACATGCTGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGccc                                                                                        >  1:1435495/1‑150 (MQ=255)

                                                             ccAACGCCGCCCTCACCCGGACATGCTGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTTCACCT‑GGCCACCCAGGCCCAAGAAGCCct                                                                                       >  1:1378350/1‑150 (MQ=255)

                                                               aaCGCCGCCCTCACCCGGACATGCTGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGCCctgc                                                                                     <  1:1487493/150‑1 (MQ=255)

                                                               aaCGCCGCCCTCACCCGGACATGCTGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGCCctgc                                                                                     >  1:2119847/1‑150 (MQ=255)

                                                               aaCGCCGCCCTCACCCGGACATGCTGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGCCctgc                                                                                     >  1:602194/1‑150 (MQ=255)

                                                                 cgccgcCCTCACCCGGACATGCTGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGCCctgctg                                                                                   <  2:3019113/150‑1 (MQ=255)

                                                                   ccgccCTCACCCGGACATGCTGGCCCCGCCACCGCCGTGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGCCCTGCTGGc                                                                                 <  1:939468/150‑1 (MQ=255)

                                                                   ccgccCTCACCCGGACATGCTGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGCCCTGCTGGc                                                                                 <  1:2739959/150‑1 (MQ=255)

                                                                       ccTCACCCGGACATGCTGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGCCCTGCTGGCCTGc                                                                             >  1:1161357/1‑150 (MQ=255)

                                                                        cTCACCCGGACACGCTGGCCCCGCCAACGCCCAGCAGCGACGACCTGGCACGCTGGCGCACGTTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGCCCTGCTGGCCTGCg                                                                            <  2:1498751/150‑1 (MQ=255)

                                                                         tCACCCGGACATGCTGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCAc                                                                                                                               <  1:313844/99‑1 (MQ=255)

                                                                         tCACCCGGACATGCTGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCAc                                                                                                                               >  2:313844/1‑99 (MQ=255)

                                                                             ccGGACATGCTGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAgg                                                                                                    <  2:2867842/121‑1 (MQ=255)

                                                                             ccGGACATGCTGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAgg                                                                                                    >  1:2867842/1‑121 (MQ=255)

                                                                             ccGGACATGCTGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGccc                                                                                        >  2:1262864/1‑133 (MQ=255)

                                                                             ccGGACATGCTGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGCCCTGCTGGc                                                                                 >  2:258645/1‑140 (MQ=255)

                                                                             ccGGACATGCTGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGCCCTGCTGGc                                                                                 <  1:258645/140‑1 (MQ=255)

                                                                             ccGGACATGCTGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGCCCTGCTGGCCTGCGGCATg                                                                       >  2:2773306/1‑150 (MQ=255)

                                                                             ccGGACATGCTGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGCCCTGCTGGCCTGCGGCATg                                                                       >  2:2993446/1‑150 (MQ=255)

                                                                               ggACATGCTGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCaag                                                                                              <  2:649787/125‑1 (MQ=255)

                                                                               ggACATGCTGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCaag                                                                                              >  1:649787/1‑125 (MQ=255)

                                                                               ggACATGCTGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGCCCTGCTGGCCTGCGGCATGCa                                                                     >  2:2263057/1‑150 (MQ=255)

                                                                                gACATGCTGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGCCCTGCTGGCCTGCGGCATGCaa                                                                    <  2:26363/150‑1 (MQ=255)

                                                                                   aTGCTGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGCCCTGCTGGCCTGCGGCATGCAACGc                                                                 <  2:602194/150‑1 (MQ=255)

                                                                                     gCTGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGCCCTGCTGGCCTGCGGCATGCAACGcat                                                               <  2:1435495/150‑1 (MQ=255)

                                                                                      cTGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGCCCTGCTGGCCTGCGGCATGCAACGcatc                                                              >  1:2417149/1‑150 (MQ=255)

                                                                                       tGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGCCCTGCTGGCCTGCGGCATGCAACGcatca                                                             <  2:1752623/150‑1 (MQ=255)

                                                                                       tGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCCGGCGCACATGGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGCCCTGCTGGCCTGCGGCATGCAACGcatca                                                             <  1:806406/150‑1 (MQ=255)

                                                                                         gCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGCCCTGCTGGCCTGCGGCATGCAACGCATCAtg                                                           >  1:165885/1‑150 (MQ=255)

                                                                                         gCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGCCCTGCTGGCCTGCGGCATGCAACGCATCAtg                                                           >  1:1448654/1‑150 (MQ=255)

                                                                                         gCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGCCCTGCTGGCCTGCGGCATGCAACGCATCAtg                                                           >  2:2728076/1‑150 (MQ=255)

                                                                                          ccccccCACCCCCCAACAGCGACGACCTGGCACGCTGGCTCACATTGTTCCAGCACCTGCTGCCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGCCCCAACAAGCCCTGCTGCCCTGCGGCATGCAACGCATCAtgc                                                          <  2:1005654/150‑1 (MQ=255)

                                                                                           cccGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGCCCTGCTGGCCTGCGGCATGCAACGCATCAtgct                                                         >  2:307400/1‑150 (MQ=255)

                                                                                            ccGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGCCCTGCTGGCCTGCGGCATGCAACGCATCAtgctg                                                        >  1:1758781/1‑150 (MQ=255)

                                                                                              gCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGcc                                                                                                                                                >  2:1551199/1‑61 (MQ=255)

                                                                                              gCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCAGTTCACCAACGCCGGGCACCT‑GGCCACCCAGGCCCAAGAAGCCCTGCTGGCCTGCGGCATGCAACGCATCATgctgct                                                      >  1:1143638/1‑150 (MQ=255)

                                                                                                 aCCGCCCAGCAGCGACGACCTGGCACGCTGCAGAGTATAGGGAAAGGATGGGCTGGCGCAGCACAGCCGGTGGACC‑ACGGTTTCCACTTCCGCCTCGCAGGCCCAAGAAGCCCTGCTGGCCTGCGGCATGCAGAACATCATGCTTCTCAg                                                   >  1:701642/1‑150 (MQ=255)

                                                                                                    gCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGCCCTGCTGGCCTGCGGCATGCAACGca                                                                <  2:345409/134‑1 (MQ=255)

                                                                                                    gCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGCCCTGCTGGCCTGCGGCATGCAACGca                                                                >  1:345409/1‑134 (MQ=255)

                                                                                                    gCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGCCCTGCTGGCCTGCGGCATGCAACGCATCATGCTGCTCAGCCt                                                >  2:1999545/1‑150 (MQ=255)

                                                                                                     cccAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCAGGTGCTGGCACaat                                                                                                                                            >  1:249540/1‑57 (MQ=25)

                                                                                                      ccAGCAGCGACGACCTGGCACGCTGGCGGACATTGTGCCAGCACCTGCTGGCCCAACCCAGACGGTTCACCAACGCGGTGCACAT‑GGCCACGCAGGCCCAAGAAGCCCTGCTGACCTGCGGCATGCAACGCATCATGCTGCTCAGCCTgg                                              >  1:1285067/1‑150 (MQ=255)

                                                                                                       cagcagCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCATGCACCT‑GGCCACCCAGGCCCAAGAAGCCCTGCTGGCCTGCGGCATGCAACGCATCATGCTGCTCAGCCTGGa                                             <  2:2819912/150‑1 (MQ=255)

                                                                                                         gcagcGACGACCTGGCACGCTGGCGCACATTGAGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCAGCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGCCCTGCTGGCCTGCGGCATGCAACGCATCATGCTGCTCAGCCTGGACa                                           >  1:1083856/1‑150 (MQ=255)

                                                                                                          cagcGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGCCCTGCTGGCCTGCGGCATGCAACGCATCATGCTGCTCAGCCTGGACaa                                          <  1:2818346/150‑1 (MQ=255)

                                                                                                           agcGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGCCCTGCTGGCCTGCGGCATGCAACGCATCATGCTGCTCAGCCTGGACAAg                                         <  1:2728076/150‑1 (MQ=255)

                                                                                                           agcGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGCCCTGCTGGCCTGCGGCATGCAACGCATCATGCTGCTCAGCCTGGACAAg                                         <  2:731991/150‑1 (MQ=255)

                                                                                                            gcGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGCCCTGCTGGCCTGCGGCATGCAACGCATCATGCTGCTCAGCCTGGACAAgg                                        <  2:2119847/150‑1 (MQ=255)

                                                                                                                cgacCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGCCCTGCTGGCCTGCGGCATGCAACGCATCATGCTGCTCAGCCTGGACAAGGCCAg                                    <  1:2993446/150‑1 (MQ=255)

                                                                                                                 gacCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGCCCTGCTGGCCTGCGGCATGCAACGCATCATGCTGCTCAGCCTGGACAAGGCCAGc                                   >  1:451886/1‑150 (MQ=255)

                                                                                                                   ccTGGCACGCTGGCGCACATTGGGCCAGCAGGTGCTGGCACaat                                                                                                                                            <  2:510483/44‑2 (MQ=2)

                                                                                                                    cTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCTGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGCCCTGCTGGCCTGCGGCATGCAACGCATCATGCTGCTCAGCCTGGACAAGGCCAGCGAc                                >  1:704028/1‑150 (MQ=255)

                                                                                                                    cTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGCCCTGCTGGCCTGCGGCATGCAACGCATCATGCTGCTCAGCCTGGACAAGGCCAGCGAc                                >  1:2281886/1‑150 (MQ=255)

                                                                                                                       gCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGCCCTGCTGGCCTGCGGCATGCAACGCATCATGCTGCTCAGCCTGGACAAGGCCAGCGACTAc                             <  2:2587614/150‑1 (MQ=255)

                                                                                                                           gCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGCCCTGCTGGCCTGCGGCATGCAACGCATCATGCTGCTCAGCCTGGACaa                                          <  1:666781/133‑1 (MQ=255)

                                                                                                                           gCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGCCCTGCTGGCCTGCGGCATGCAACGCATCATGCTGCTCAGCCTGGACAAGGCCAGCGACTACCTgc                         <  1:2263057/150‑1 (MQ=255)

                                                                                                                           gCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGCCCTGCTGGCCTGCGGCATGCAACGCATCATGCTGCTCAGCCTGGACAAGGCCAGCGACTACCTgc                         >  2:3041201/1‑150 (MQ=255)

                                                                                                                             tGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGCCCTGCTGGCCTGCGGCATGCAACGCATCATGCTGCTCAGCCTGGACAAGGCCAGCGACTACCTgcgc                       <  1:1219088/150‑1 (MQ=255)

                                                                                                                                  cacaTTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGCCCTGCTGGCCTGCGGCATGCAACGCATCATGCTGCTCAGCCTGGACAAGGCCAGCGACTACCTGCGCGTgca                  <  1:2393783/150‑1 (MQ=255)

                                                                                                                                   acaTTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGCCCTGCTGGCCTGCGGCATGCAACGCATCATGCTGCTCAGCCTGGACAAGGCCAGCGACTACCTGCGCGTgcag                 >  1:1936017/1‑150 (MQ=255)

                                                                                                                                     aTTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCaa                                                                                               <  2:349782/70‑1 (MQ=255)

                                                                                                                                     aTTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCaa                                                                                               >  1:349782/1‑70 (MQ=255)

                                                                                                                                           ccAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCaa                                                                                               >  2:242390/1‑64 (MQ=255)

                                                                                                                                           ccAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCaa                                                                                               <  1:242390/64‑1 (MQ=255)

                                                                                                                                           ccAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGCCCTGCTGGCCTGCGGCATGCAACGCATCATGCTGCTCAGCCTGGACAAGGCCAGCGACTACCTGCGCGTGCCGCAAAtttc         >  1:1411630/1‑148 (MQ=255)

                                                                                                                                           ccAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGCCCTGCTGGCCTGCGGCATGCAACGCATCATGCTGCTCAGCCTGGACAAGGCCAGCGACTACCTGCGCGTGCAGCAAATTGc         <  2:2388785/150‑1 (MQ=255)

                                                                                                                                               cACCTGCTGGCCCAACCCAGCCCGTTCACCACCGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGCCCTGCTGGCCTGCGGCATGCAACGCATCATGCTGCTCAGCCTGGACAAGGCCAGCGACTACCTGCGCGTGCAGCAAATTGCCGGc     <  2:1843231/150‑1 (MQ=255)

                                                                                                                                                  ctgctgGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGCCCTGCTGGCCTGCGGCATGCAACGCATCATGCTGCTCAGCCTGGCCAAGGCCAGCCCATCCCTGCGCGGGCAGCAGATTTCCGGCCTg  >  2:2191251/1‑150 (MQ=255)

CCCGCCGCCACGCCCGGCACGCCCTGTTCCACCCGGCCGAGGCCTTGATCTGGCCGTGGCACCAACGCCGCCCTCACCCGGACATGCTGGCCCCGCCACCGCCCAGCAGCGACGACCTGGCACGCTGGCGCACATTGTGCCAGCACCTGCTGGCCCAACCCAGCCCGTTCACCAACGCCGTGCACCT‑GGCCACCCAGGCCCAAGAAGCCCTGCTGGCCTGCGGCATGCAACGCATCATGCTGCTCAGCCTGGACAAGGCCAGCGACTACCTGCGCGTGCAGCAAATTGCCGGCCTG  >  NC_002947/5577252‑5577547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`