Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,651,428 C→A 9.5% intergenic (‑185/+28) PP_4959 ← / ← fba diguanylate cyclase/fructose‑bisphosphate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,651,4280CA 9.5% 432.0 / 20.6 137intergenic (‑185/+28)PP_4959/fbadiguanylate cyclase/fructose‑bisphosphate aldolase
Reads supporting (aligned to +/- strand):  ref base C (69/55);  new base A (6/7);  total (75/62)
Fisher's exact test for biased strand distribution p-value = 5.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01

Only 100 of 137 total aligned reads displayed.

GTTGGATCCATTAGTACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAGGCTTCGTAACGCGCGATGC  >  NC_002947/5651280‑5651574
                                                                                                                                                    |                                                                                                                                                  
      tCCATTAGTACTCTAGACCTGAGGCTGCAACACGCAATGTAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCAGCGATGCGGGt                                                                                                                                             <  1:1674913/150‑1 (MQ=255)
      tCCATTAGTACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCAGCGATGCGGGt                                                                                                                                             <  2:653267/150‑1 (MQ=255)
         aTTAGTACTCTAGACGTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGAAATTATGTACAGGTTTCCATCTACCATAAAAAAACCCGCCTCGCTGCGGGtttt                                                                                                                                          >  2:843165/1‑150 (MQ=255)
         aTTAGTACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGtttt                                                                                                                                          >  1:747641/1‑150 (MQ=255)
         aTTAGTACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGtttt                                                                                                                                          >  1:2729584/1‑150 (MQ=255)
         aTTAGTACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGtttt                                                                                                                                          >  1:2496738/1‑150 (MQ=255)
             gTACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGATGCGGGTTTTTCAg                                                                                                                                      <  2:2850856/150‑1 (MQ=255)
              tACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAgg                                                                                                                                     <  1:960710/150‑1 (MQ=255)
               aCTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAggg                                                                                                                                    <  1:42988/150‑1 (MQ=255)
                      aCCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTg                                                                                                                             <  2:1401965/150‑1 (MQ=255)
                       ccTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGTGGGGTTTTCAGGGTTACCTGt                                                                                                                            >  1:2459582/1‑150 (MQ=255)
                        cTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAgg                                                                                                                                     >  1:1146993/1‑140 (MQ=255)
                        cTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAgg                                                                                                                                     <  2:1146993/140‑1 (MQ=255)
                        cTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTc                                                                                                                           <  1:1205351/150‑1 (MQ=255)
                             gCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCa                                                                                                                      <  1:86538/150‑1 (MQ=255)
                             gCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCa                                                                                                                      >  1:2886263/1‑150 (MQ=255)
                               tGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGt                                                                                                                    >  2:2546232/1‑150 (MQ=255)
                                gCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGtt                                                                                                                   <  2:4924/150‑1 (MQ=255)
                                  aaCACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTAc                                                                                                                                >  2:2900853/1‑135 (MQ=255)
                                  aaCACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTAc                                                                                                                                <  1:2900853/135‑1 (MQ=255)
                                  aaCACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGa                                                                                                                 <  2:2729584/150‑1 (MQ=255)
                                  aaCACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGa                                                                                                                 <  2:747641/150‑1 (MQ=255)
                                   acacGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGAc                                                                                                                >  1:2811571/1‑150 (MQ=255)
                                     acGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCt                                                                                                              >  1:2128958/1‑150 (MQ=255)
                                     acGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCt                                                                                                              >  1:768906/1‑150 (MQ=255)
                                     acGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCt                                                                                                              >  2:3001651/1‑150 (MQ=255)
                                       gcaatgcaatCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTg                                                                                                            <  2:2894104/150‑1 (MQ=255)
                                         aatgcaatCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCTTGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTggc                                                                                                          >  1:2333736/1‑150 (MQ=255)
                                         aatgcaatCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAg                                                                                                                                      >  2:754280/1‑122 (MQ=255)
                                         aatgcaatCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAg                                                                                                                                      <  1:754280/122‑1 (MQ=255)
                                         aatgcaatCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACACGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACATTggg                                                                                                          >  1:1233469/1‑149 (MQ=255)
                                            gcaatCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTggcggc                                                                                                       <  2:2161864/150‑1 (MQ=255)
                                             caatCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGcc                                                                                                      >  1:3040609/1‑150 (MQ=255)
                                              aatCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAg                                                                                                                                      <  1:597705/117‑1 (MQ=255)
                                              aatCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAg                                                                                                                                      >  2:597705/1‑117 (MQ=255)
                                                tCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTa                                                                                                                                 <  2:1111382/120‑1 (MQ=255)
                                                tCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGt                                                                                                   >  2:2717108/1‑150 (MQ=255)
                                                   gAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCa                                                                                                <  2:2128958/150‑1 (MQ=255)
                                                    aaCGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCAc                                                                                               >  1:823326/1‑150 (MQ=255)
                                                       gCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCtt                                                                                            >  1:1382636/1‑150 (MQ=255)
                                                         tATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTc                                                                                          <  2:2480600/150‑1 (MQ=255)
                                                          aTCAATGATCAAACTGTACGGTAGCCGACCAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCa                                                                                         <  2:3020240/150‑1 (MQ=255)
                                                             aaTGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGt                                                                                      >  2:1048349/1‑150 (MQ=255)
                                                             aaTGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGt                                                                                      >  1:2047088/1‑150 (MQ=255)
                                                                gATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTTTCGAt                                                                                                                        >  2:2039048/1‑113 (MQ=255)
                                                                    aaaCTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTg                                                                               <  2:23224/150‑1 (MQ=255)
                                                                     aaCTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAgc                                                                                   <  1:1663722/145‑1 (MQ=255)
                                                                        tGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCGGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTAc                                                                           >  2:2435253/1‑150 (MQ=255)
                                                                         gTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACa                                                                          <  2:823326/150‑1 (MQ=255)
                                                                          tACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCGGCGGGTTTTTCAGGGTTACCTGTc                                                                                                                           >  1:372312/1‑100 (MQ=255)
                                                                                 gCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGCTCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTcc                                                                  <  2:1747306/150‑1 (MQ=255)
                                                                                    gACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCaga                                                               <  1:1013314/150‑1 (MQ=255)
                                                                                    gACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCaga                                                               <  1:2717108/150‑1 (MQ=255)
                                                                                     aCTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTAc                                                                                                                                <  2:1638444/84‑1 (MQ=255)
                                                                                          cggtcggtCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATc                                                         >  1:2526687/1‑150 (MQ=255)
                                                                                             tctgtcTGCCGGAATTATGTACAGGTTTCCAGCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATAAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGc                                                      <  2:1523415/147‑1 (MQ=255)
                                                                                             tcggtcTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGc                                                      >  2:2415203/1‑150 (MQ=255)
                                                                                              cggtcTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCt                                                     <  2:2496738/150‑1 (MQ=255)
                                                                                                    gCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCt                                               <  1:479798/150‑1 (MQ=255)
                                                                                                     ccGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGa                                                                                                                 >  1:1691418/1‑83 (MQ=255)
                                                                                                     ccGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGa                                                                                                                 <  2:1691418/83‑1 (MQ=255)
                                                                                                           ttATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCaga                                                               >  2:2328083/1‑127 (MQ=255)
                                                                                                           ttATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCaga                                                               <  1:2328083/127‑1 (MQ=255)
                                                                                                           ttATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGc                                        >  1:1223081/1‑150 (MQ=255)
                                                                                                             aTGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCTAGGCAt                                      <  1:1048349/150‑1 (MQ=255)
                                                                                                                tccAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGc                                   >  2:1654784/3‑150 (MQ=255)
                                                                                                                tACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCACGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGGGCTGGTACATGCCTTCCAGAAAGATCGGCTTGATCTTCGAGGCCTTGc                                   >  2:412652/1‑150 (MQ=255)
                                                                                                                 aCAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGcc                                  >  2:2766219/1‑150 (MQ=255)
                                                                                                                 aCAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGcc                                  >  1:2052485/1‑150 (MQ=255)
                                                                                                                   aGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAg                                                                                                    >  1:1581287/1‑82 (MQ=255)
                                                                                                                   aGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAg                                                                                                    <  2:1581287/82‑1 (MQ=255)
                                                                                                                     gTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCcgcg                              <  1:3001651/150‑1 (MQ=255)
                                                                                                                         ccATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGc                          >  2:1152478/1‑150 (MQ=255)
                                                                                                                         ccATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGc                          <  1:176478/150‑1 (MQ=255)
                                                                                                                          cATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGcc                         >  1:2028230/1‑150 (MQ=255)
                                                                                                                          cATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGcc                         >  2:1089420/1‑150 (MQ=255)
                                                                                                                           aTCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCg                        >  1:1075967/1‑150 (MQ=255)
                                                                                                                            tctcCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTAACTGCCTTTACGGGAAATCGGCTTGCTCTTCGAGGCATTGCCCGCGGTGCCgg                       >  1:1570152/1‑149 (MQ=255)
                                                                                                                              tcCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCt                                                                                <  2:318551/91‑1 (MQ=255)
                                                                                                                              tcCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCt                                                                                >  1:318551/1‑91 (MQ=255)
                                                                                                                               cccATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAgg                    <  1:2766219/150‑1 (MQ=255)
                                                                                                                                ccATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAg                                                                                                                     >  1:613953/1‑52 (MQ=255)
                                                                                                                                ccATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAg                                                                                                    <  1:3055539/69‑1 (MQ=255)
                                                                                                                                ccATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAg                                                                                                    >  2:3055539/1‑69 (MQ=255)
                                                                                                                                  aTAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTc                                             >  2:2398354/1‑122 (MQ=255)
                                                                                                                                  aTAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTc                                             <  1:2398354/122‑1 (MQ=255)
                                                                                                                                  aTAAAAAAACCCGCATCGCTGCGGGCGTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCAAGAGACAGCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAGGCtt                 >  2:404983/1‑150 (MQ=255)
                                                                                                                                   tAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCtt                                                    >  1:2109136/1‑114 (MQ=255)
                                                                                                                                   tAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCtt                                                    <  2:2109136/114‑1 (MQ=255)
                                                                                                                                   tAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAGGCTTc                <  1:1152478/150‑1 (MQ=255)
                                                                                                                                   tAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAGGCTTc                <  2:2063140/150‑1 (MQ=255)
                                                                                                                                    tgaaaaaCCCGCAGCGATGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAGGCTTCg               >  2:507908/3‑150 (MQ=255)
                                                                                                                                     gaaaaaCCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAGGCTTCGt              >  1:263163/2‑150 (MQ=255)
                                                                                                                                      aaaaaCCCGCAGCGATGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCt                                                                     <  1:2004663/94‑1 (MQ=255)
                                                                                                                                      aaaaaCCCGCAGCGATGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCt                                                                     >  2:2004663/1‑94 (MQ=255)
                                                                                                                                        aaaCCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGc                          >  1:208035/1‑135 (MQ=255)
                                                                                                                                        aaaCCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAGGCTTCGTAAc           >  2:2586038/1‑150 (MQ=255)
                                                                                                                                            ccGCATCGCTGCGGGTTTTTCGGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAGGCTTCGTAAcgcgc       <  1:2435253/150‑1 (MQ=255)
                                                                                                                                            ccGCATCGCTGCGGGTTTTTCAGGGTTACGGTTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAGGCTTCGTAAcgcgc       >  1:2386590/1‑150 (MQ=255)
                                                                                                                                            ccGCAGCGATGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAGGCTTCGTAAcgcgc       >  2:147878/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GTTGGATCCATTAGTACTCTAGACCTGAGGCTGCAACACGCAATGCAATCAGAACGCTATCAATGATCAAACTGTACGGTAGCCGACTAACGGTCGGTCTGCCGGAATTATGTACAGGTTTCCATCTCCCATAAAAAAACCCGCATCGCTGCGGGTTTTTCAGGGTTACCTGTCGATCAGTTGACCTTGGCGGCCAGTTCACCTTTCAGGTAGCGCTGGTACATGCCTTCCAGAGAGATCGGCTTGATCTTCGAGGCATTGCCCGCGGTGCCGAAGGCTTCGTAACGCGCGATGC  >  NC_002947/5651280‑5651574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑