Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,223,0470CG55.2% ‑5.5 / 18.3 29R433P (CGG→CCG) PP_4603transposase
Reads supporting (aligned to +/- strand):  ref base C (0/13);  new base G (0/16);  total (0/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GCGACAGCGCAGCCCGGAAGGGCTGGGTGATCTCTCATCAAACAAAATGCAACCCA‑TTGTTTTCTAAGCACTCAAGCGGCCACCAGTTGATACCCCAGTTTCCGGGCTTTGCGAAGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACAGGTTGTACACCAGACGTGCCAACTGATGCGCAGTGGCCTTGATGGCGC  >  NC_002947/5222945‑5223191
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gctactgcgcAGCCCGGATGGGCTGGGTGATCTCACACCAAACAAAATGAAACCCT‑TTGTTTTCTAAGCACTCAAGCGGCCACCATTTGATACCCCTGTTTCGGGGATTTGCTAAGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGtt                                                                                                   <  2:3695839/144‑1 (MQ=1)
             ccggtcgggCTGGGTGATCTCTCATCAAACAAAATTCAACCCT‑TTTTTTTCTAACCAAACACGCGGCCACCATTTTATACCCCTGTTTCGGGGCTTTGCGAAGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCaa                                                                                      <  2:843140/144‑1 (MQ=1)
                    gggtGGTTGATCTCTCATAAAACAAAATGCACCCCA‑TTGTTTTCTAAGCACTCACGCGGCCACCAGTTGATACCCCTGTTTCGGGGCTTTGCGAAGAAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTg                                                                               <  2:4657356/147‑1 (MQ=1)
                          gttaTCTCTCATCAAACAAAATCCAACCCA‑TTGTTTTCTAAGCACTCAAGCGGCCACCTTTTGATACCCCAGTTTCCGGGCTTTGCGAAGAAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAAc                                                                         <  2:5067557/147‑1 (MQ=1)
                          gttaTCTCTCAACAAAAAAAAGGCAACCAT‑TTTTTTTCTACGCACTCACGCGGCCCCCAGTTGATACCCCAGTTTCCGGGATTTGCGAAGAAACGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAAc                                                                         <  1:4932760/147‑1 (MQ=1)
                                tcccatcaAAAAAATTGCAACCCT‑TTGTTTTCTACGCACTCCCGGGGCCCCCCGTTGATCCCCCTTTTTCGGGGGTTTGCGAAGCAACGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGc                                                                   <  1:2983572/147‑1 (MQ=1)
                                    aaaaaccaaaATGCAACCCT‑TTGTTTTCTAAGCACTCAAGCGGCCACCAGTTGATACCCCAGTTTCGGGGCTTTGCGAAGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGc                                                               <  1:5006394/144‑1 (MQ=0)
                                      cacacaaaATCCACCCCC‑TTTTTTTCAAAGAACTCACGCGGCCCCCAGTGGATACCCCATTTGCCGGGCTTTGCGAAGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGctt                                                             <  1:4951501/147‑1 (MQ=1)
                                         accaaaGGCAACCCC‑TTGTTTTCTAAGCACTCCCGGGGCCACCTGTTGAAACCCCTGTTTCCGGGCTTTGCGAAGCACCGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGcttctt                                                          <  2:1153003/147‑1 (MQ=1)
                                           aaaaaccAACCCATTTTTTTTCTAACCACACACGCGGCCACCTTTTCAACCCCCTGTTGCGGGGTTTTGCCACCCAACCCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTg                                                         <  1:1723362/144‑1 (MQ=0)
                                                 caccccT‑TTGTTTTCTAAGCACTCAAGCGGCCACCTGTTGATACCCCTGTTGCCGGGCTTGGCGAAGCAATGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACa                                                  <  2:2646873/147‑1 (MQ=1)
                                                 caccccT‑TTGTTTTCTAAGCACTCAAGCGGCCACCAGTTGATACCCCAGTTTCCGGGCTTTGCGAAGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACa                                                  <  2:1318986/147‑1 (MQ=1)
                                                 caccccA‑TTGTTTTCTACGCACCCACGCGGCCACCCGTTGATCCCCCCGTTTCCGGGCTTTGCGAAGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACa                                                  <  2:2895060/147‑1 (MQ=1)
                                                      ccttttttttCTACCCACCCCCGCGCCCACCCGTTGATCCCCCTGTTTCGGGTCTTGGCGACCAAACCCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACAGGtt                                              <  1:850857/144‑1 (MQ=1)
                                                      ccttttttttCTACCAACACACGCGGCCACCTTTTTAAACCCCTTTTGCGGGTCTTTGCGAAGCAACCCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACAGGtt                                              <  1:4124101/144‑1 (MQ=1)
                                                      cctttGTTTTCTACGCACCCCCGCGGCCCCCCGTTGCTACCCCAGTGGCGGGGCTTTGCGACGCAACGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACAGGtt                                              <  2:1671363/147‑1 (MQ=1)
                                                      cctttGTTTTCTAAGCACTCCAGGGGCCACCCGTTGAGACCCCTGGTTCCGGGGGTTGCGAAGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACAGGtt                                              <  2:5329324/147‑1 (MQ=1)
                                                      cctttGTTTTCAACGCACTCACGCGGCCACCTGTTGATACCCCTGTTTCGGGGCTTTGCTACGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACAGGtt                                              <  2:4026177/147‑1 (MQ=1)
                                                      cccttGTTTTCTCCGCACCCACGCGGCCACCCGTGGAGACCCCCGTTTCGGGGCTTTGCGAAGCAACGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACAGGtt                                              <  2:3246502/147‑1 (MQ=1)
                                                       cttttttttCTATCCACACAGGCGCCCACCCGTCCTTCCCCCTTTTGCGGGGCTTGGCGACCAAACCCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACAGGTTg                                             <  1:4162289/145‑1 (MQ=0)
                                                       cttttttttCTAAGCACTCACGGGGCCACCAGTTGATACCCCCGTTTCCGGGCTTTGCGAAGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACAGGTTg                                             <  1:3482870/145‑1 (MQ=0)
                                                                   agaaCTCAAGCGGCCACCTTTTTATACCCCAGTTTCGGGGGTTTGCTAAGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACAGGTTGTACACCAGACGt                                 <  2:895083/147‑1 (MQ=1)
                                                                      acacacgcgcCCACCTGTTGAAACCCCTGTTTCGGGGCTTTGCGATGAAAACCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACAGGTTGTACACCAGACGTGcc                              <  1:4761147/144‑1 (MQ=1)
                                                                         cacgCGGCCCCCTTTTGACACCCATGTTGCGGGGCTTTCCGAACAAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACAGGTTGTACACCAGACGTGCCAAc                           <  1:5099522/147‑1 (MQ=1)
                                                                                       tttaTACCCCCGTTTCCGGGCTTTGCGAAGCAACGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACAGGTTGTACACCAGACGTGCCAACTGATGCGCAGTGGc             <  2:4562375/147‑1 (MQ=0)
                                                                                          atcccccAGTTGCCGGGCTTGGCGACGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACAGGTTGTACACCAGACGTGCCAACTGATGCGCAGTGGCCtt          <  1:2488571/147‑1 (MQ=0)
                                                                                             cccctttttCGGGGTTTTGCTAAGAAACCCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACAGGTTGTACACCAGACGTGCCAACTGATGCGCAGTGGCCTTGAt       <  1:3055602/144‑1 (MQ=1)
                                                                                                 cgtctCCGGGCTTTGCGACGCACAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACAGGTTGTACACCAGACGTGCCAACTGATGCGCAGTGGCCTTGATGgcg   <  2:4076996/146‑1 (MQ=0)
                                                                                                  gtgtCCGGGCTTTGCGAAGAAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACAGGTTGTACACCAGACGTGCCAACTGATGCGCAGTGGCCTTGATGgcgc  <  1:5375619/147‑1 (MQ=1)
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GCGACAGCGCAGCCCGGAAGGGCTGGGTGATCTCTCATCAAACAAAATGCAACCCA‑TTGTTTTCTAAGCACTCAAGCGGCCACCAGTTGATACCCCAGTTTCCGGGCTTTGCGAAGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGTTTCGAACTCCTCAAGACCTTGTTCAACATAAGCCTGCTTCTTGGTTAACAGGTTGTACACCAGACGTGCCAACTGATGCGCAGTGGCCTTGATGGCGC  >  NC_002947/5222945‑5223191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑