Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,852,269	C→T	A438V (GCC→GTC)	relA →	ATP:GTP 3'‑pyrophosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,852,269	0	C	T	100.0%	88.1 / NA	31	A438V (GCC→GTC)	relA	ATP:GTP 3'‑pyrophosphotransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/13);  total (18/13)

TGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAGCCGTGACTGGTT  >  NC_002947/1852125‑1852413                                                                                                                                                 |                                                                                                                                                 tGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTAcc                                                                                                                                             <  2:532100/150‑1 (MQ=255)             aCGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCg                                                                                                                                 >  2:96853/1‑150 (MQ=255)                                 ggTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTa                                                                                                                                               >  2:61297/1‑116 (MQ=255)                                 ggTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTa                                                                                                                                               <  1:61297/116‑1 (MQ=255)                                   tggcctggccGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCgcgg                                                                                                           >  1:9424/1‑150 (MQ=255)                                     gcctggccGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCgcggcg                                                                                                         >  1:649158/1‑150 (MQ=255)                                         ggccGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGaa                                                                                                     <  1:534961/150‑1 (MQ=255)                                                        ggTCGACATCGAGCCTGACCGGGTTTATGTGTTCACACCCCTGGATCACGCTATCGACCTGCCCAAAGGCTCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCTCGGCGCGAAGATCAACGGCCGTAt                                                                                      >  1:479022/1‑150 (MQ=255)                                                             aCATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGc                                                                                 <  2:479022/150‑1 (MQ=255)                                                              cATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGAATTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGcc                                                                                <  2:574465/150‑1 (MQ=255)                                                                    tccTGCCCGGGTTTATGTGTTCCCCCCCGCCGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGaa                                                                          <  1:64711/149‑1 (MQ=255)                                                                       tGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACtg                                                                       >  2:28799/1‑149 (MQ=255)                                                                            tgttctATGTGTTCACCCCCGACGCCCACCCCATGCCCCTCCCCGAAGCCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACCGCCGTATCGTGCCGCTGAACTACAGcc                                                                  <  2:269719/145‑1 (MQ=255)                                                                             ggTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCt                                                                 <  2:261989/150‑1 (MQ=255)                                                                              gTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTg                                                                >  1:32616/1‑150 (MQ=255)                                                                               tttATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGc                                                               <  2:32616/150‑1 (MQ=255)                                                                                ttATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCa                                                              >  1:26913/1‑150 (MQ=255)                                                                                  aTGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCACAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCCCGGCCCGAAGATCAACAGCCGCATCGTGCCGCCGAACCACAGCCTGCAGa                                                            >  2:132732/1‑150 (MQ=255)                                                                                         cACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGa                                                     <  1:530721/150‑1 (MQ=255)                                                                                          aCCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGCTCGGCCACAACTGCCGCGGCCCGAAGATCAACGCCCACATAGTGCCGCTGACGGACCCCCTGCCGCCTGGCGAg                                                    >  2:93029/1‑150 (MQ=255)                                                                                          aCCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAg                                                    >  1:224580/1‑150 (MQ=255)                                                                                            ccccGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGAACGGCCACAACCGCCGCGGCGCGACGCTCAACCGACGTATCGTACCACCGACCGACAGCCGGCAGACCGGCGAGCa                                                  >  2:184263/1‑150 (MQ=255)                                                                                             cccGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAg                                                 >  1:525296/1‑150 (MQ=255)                                                                                              ccGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAgg                                                >  1:690499/1‑150 (MQ=255)                                                                                                gggggccaggccaTCGAACCGCCCACGCCCGGCACGCCATTAGACTTGGTCTACCGCGTGGACACCGCGGTCGGCCACAACTGCCGCGGCGCGGCGCGCAACGACCGTAGCACTCCGCGGACCTTCAGCCCGCAGACTAACGAGCAgagg                                              >  2:141985/4‑147 (MQ=255)                                                                                                     ccACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGat                                         <  2:9424/150‑1 (MQ=255)                                                                                                           cATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCAc                                   <  1:96853/150‑1 (MQ=255)                                                                                                                  cTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCACACTGGCGAGCAGGTGGAGATCATCACCagcaag                            <  1:269927/150‑1 (MQ=255)                                                                                                                        aaaGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGc                      >  1:531842/1‑150 (MQ=255)                                                                                                                              gccacgccaTTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAAcccc                >  1:631451/1‑150 (MQ=255)                                                                                                                                            cTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGCACTACAGCCTGCAGCGTGGAGACCAGGCGGGGATCATCACAAGCAAGCCCGTCAACCGCAACCTTGACTGGtt  >  2:41643/1‑150 (MQ=255)                                                                                                                                                 |                                                                                                                                                 TGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAGCCGTGACTGGTT  >  NC_002947/1852125‑1852413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑