| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 191,912 | Δ1 bp | 7.0% | coding (1606/2157 nt) | paxB ← | toxin secretion ATP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 191,912 | 0 | C | . | 7.0% | 184.4 / 8.1 | 57 | coding (1606/2157 nt) | paxB | toxin secretion ATP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base C (28/25); new base . (2/2); total (30/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.07e-01 | |||||||||||
CGGGCGCCGCTGACCAGGTTGTCACGCAAGGTGCCGGCCAGCAGCTGGATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTCTGCTGGTTCGGATAAGTGAAGTCGACCCCGCG > NC_002947/191767‑192059 | cGGGCGCCGCTGACCAGGTTGTCACGCAAGGTGCCGGCCAGCAGCTGGATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAc > 2:1511751/1‑150 (MQ=255) cgcTGACCAGGTTGTCACGCAAGGTGCCGGCCAGCAGCTGGATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCcga > 1:1448970/1‑150 (MQ=255) tGACCAGGTTGTCACGCAAGGTGCCGGCCAGCAGCTGGATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCcgacga < 1:1620330/150‑1 (MQ=255) gACCAGGTTGTCACGCAAGGTGCCGGCCAGCAGCTGGATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGAt < 2:29452/150‑1 (MQ=255) ccAGGTTGTCACGCAAGGTGCCGGCCAGCAGCTGGATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGa > 2:455849/1‑150 (MQ=255) cAGGTTGTCACGCAAGGTGCCGGCCAGCAGCTGGATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAg > 2:284204/1‑150 (MQ=255) ttGTCACGCAAGGTGCCGGCCAGCAGCTGGATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTg < 2:166342/150‑1 (MQ=255) ttGTCACGCAAGGTGCCGGCCAGCAGCTGGATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTg > 2:1371318/1‑150 (MQ=255) gTCACGCAAGGTGCCGGCCAGCAGCTGGATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGc < 2:1535400/150‑1 (MQ=255) gTGCCGGCCAGCAGCTGGATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTg > 1:394160/1‑138 (MQ=255) gTGCCGGCCAGCAGCTGGATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTg < 2:394160/138‑1 (MQ=255) cGGCCAGCAGCTGGATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGc > 1:914984/1‑150 (MQ=255) gcTGGATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACACCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGc > 1:538640/1‑150 (MQ=255) ggATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGc > 2:1149483/1‑150 (MQ=255) tatCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGc < 2:843954/150‑1 (MQ=255) tGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCgatg < 2:1333085/150‑1 (MQ=255) gCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCtt > 2:883565/1‑150 (MQ=255) gATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACACCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTtctc < 2:538640/150‑1 (MQ=255) gttgtGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCcg > 1:514083/1‑85 (MQ=255) gttgtGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCcg < 2:514083/85‑1 (MQ=255) ttgtGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCa < 2:1448970/150‑1 (MQ=255) ggCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGCACTCTTGCCTTAGCCGGTGCGGCCGATGATGCCGACCTTCTCGCCAGGGc < 1:284204/150‑1 (MQ=255) tCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTc < 1:883565/150‑1 (MQ=255) gACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGc < 2:735201/95‑1 (MQ=255) gACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGc > 1:735201/1‑95 (MQ=255) cATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGa > 2:1545744/1‑150 (MQ=255) aCGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTtctc > 2:421946/1‑107 (MQ=255) aCGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTtctc < 1:421946/107‑1 (MQ=255) aTCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGc > 2:640827/1‑80 (MQ=255) aTCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGc < 1:640827/80‑1 (MQ=255) aTCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGcc > 1:1551952/1‑105 (MQ=255) aTCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGcc < 2:1551952/105‑1 (MQ=255) aTCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTctgccg > 1:66524/1‑148 (MQ=255) tCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCga > 1:238540/1‑85 (MQ=255) tCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCga < 2:238540/85‑1 (MQ=255) aCCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTCTGCAGATTc < 2:1471274/150‑1 (MQ=255) agcGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCa < 2:671024/124‑1 (MQ=255) agcGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCa > 1:671024/1‑124 (MQ=255) gcGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGAt < 1:1422702/103‑1 (MQ=255) gcGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGAt > 2:1422702/1‑103 (MQ=255) cGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCTATTGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTCTGCTGGTTCGGATAAGt < 1:199972/150‑1 (MQ=255) gCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTCTGCTGGTTCGGATAAGTGaa > 1:161633/1‑150 (MQ=255) tcatcgtCGGCCT‑GTACGAGGCCGATGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTctgctg > 1:501241/7‑134 (MQ=38) tcatcgtCGGCCT‑GTACGAGGCCGATGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTctgctg < 2:501241/128‑1 (MQ=255) caccaTCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTCTGCTGGTTCGGATAAGTGAAGt > 1:198851/1‑150 (MQ=255) accaTCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTCTGCTGGTTCGGATAAGTGAAGTc < 2:154024/150‑1 (MQ=255) accaTCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTCTGCTGGTTCGGATAAGTGAAGTc > 1:154024/1‑150 (MQ=255) accaTCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTCTGCTGGTTCGGATAAGTGAAGTc < 1:1371318/150‑1 (MQ=255) accaTCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTCTGCTGGTTCGGATAAGTGAAGTc > 1:1203420/1‑150 (MQ=255) tcgtCGGCCT‑GTACGAGGCCGATGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCa > 2:347957/4‑115 (MQ=38) tcgtCGGCCT‑GTACGAGGCCGATGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCa < 1:347957/112‑1 (MQ=38) aTCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTCTGCTGGTTCGGATAAGTGAAGTCGAc < 2:198851/150‑1 (MQ=255) gCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTCTGCTGGTTCGGATAAGTGAAGTCGACCCcg > 1:1059969/1‑150 (MQ=255) ccTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTCTGCTGGTTCGGATAAGTGAAGTCGACCCcgc > 1:915548/1‑150 (MQ=255) cTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGATCTGCTGGTTCGGATAAGTGAAGTCGACCAcgcg > 1:1411474/1‑150 (MQ=255) tCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATg > 2:821833/1‑87 (MQ=255) tCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATg < 1:821833/87‑1 (MQ=255) | CGGGCGCCGCTGACCAGGTTGTCACGCAAGGTGCCGGCCAGCAGCTGGATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTCTGCTGGTTCGGATAAGTGAAGTCGACCCCGCG > NC_002947/191767‑192059 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |