Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 58,750 T→A 9.6% intergenic (‑661/+27) PP_5424 ← / ← PP_0050 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294758,7500TA 9.6% 160.6 / 7.6 52intergenic (‑661/+27)PP_5424/PP_0050hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (23/24);  new base A (2/3);  total (25/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01

GTGAGTAGGGATTTCTCGGCTTTTCTGTGAAAAGCACCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACGAGGTGGCGCATACAGGTGGCGCGCTTTCCA  >  NC_002947/58601‑58896
                                                                                                                                                     |                                                                                                                                                  
gTGAGTAGGTATTTCTAGGCTTTTCTGGGAAAAGCACCCCTTCATTTAAAAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCgctgct                                                                                                                                                    <  2:661864/150‑1 (MQ=255)
gTGAGTAGGGATTTCTCGGCTTTTCTGTGAAAAGCACCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCgctgct                                                                                                                                                    >  2:254105/1‑150 (MQ=255)
     tAGGGATTTCTCGGCTTTTCTGTGAAAAGCACCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCggg                                                                                                                                               <  1:1426479/150‑1 (MQ=255)
       gggATTTCTCGGCTTTTCTGTGAAAAGCACCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCt                                                                                                                                             >  1:570844/1‑150 (MQ=255)
          aTTTCTCGGCTTTTCTGTGAAAAGCACCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCtttt                                                                                                                                          <  2:682590/150‑1 (MQ=255)
               tcGGCTTTTCTGTGAAAAGCACCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTg                                                                                                                                     >  1:1522388/1‑150 (MQ=255)
                cGGCTTTTCTGTGAAAAGCACCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGc                                                                                                                                    <  2:155220/150‑1 (MQ=255)
                   cTTTTCTGTGAAAAGCACCCCTTCATCTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCAGCAGCGGGCTTTTTAGTGTCTg                                                                                                                                 <  1:963744/150‑1 (MQ=255)
                         tgtgAAAAGCACCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACa                                                                                                                           >  2:947026/1‑150 (MQ=255)
                          gtgAAAAGCACCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACaa                                                                                                                          <  1:947026/150‑1 (MQ=255)
                             aaaaGCACCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCa                                                                                                                       >  2:555565/1‑150 (MQ=255)
                                     cccTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTACAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCgg                                                                                                               <  1:1532132/150‑1 (MQ=255)
                                       cTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACATATTTTGTGAACATACAAGGTTGAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTg                                                                                                             >  1:198032/1‑150 (MQ=255)
                                              tCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGt                                                                                                      <  2:570844/150‑1 (MQ=255)
                                                 caAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCa                                                                                                   <  1:555565/150‑1 (MQ=255)
                                                  aaaaaaaaTGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCAc                                                                                                  <  1:1359339/150‑1 (MQ=255)
                                                  aaaaaaaaTGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCAc                                                                                                  >  2:1359339/1‑150 (MQ=255)
                                                  aaaaaaaaTGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGCGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCa                                                                                                   >  2:1173650/1‑149 (MQ=255)
                                                  aaaaaaaaTGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGCGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCa                                                                                                   <  1:1173650/149‑1 (MQ=255)
                                                  aaaaaaaaTGGCGAAGATTCCAATTTCACGGGGTATGGCCTATCACCTATTTGGTGAACCTACAAGGTGTAGTGCACCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCAc                                                                                                  <  1:366256/150‑1 (MQ=255)
                                                     aaaaaTGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTga                                                                                                            >  1:1105360/1‑137 (MQ=255)
                                                     aaaaaTGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTga                                                                                                            <  2:1105360/137‑1 (MQ=255)
                                                            gCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCAc                                                                                                  <  1:134525/140‑1 (MQ=255)
                                                            gCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCAc                                                                                                  >  2:134525/1‑140 (MQ=255)
                                                              gAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCa                                                                                                   <  1:787328/137‑1 (MQ=255)
                                                              gAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCa                                                                                                   >  2:787328/1‑137 (MQ=255)
                                                                     ccAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATg                                                                               <  1:254105/150‑1 (MQ=255)
                                                                       aaTTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGc                                                                             <  2:1522388/150‑1 (MQ=255)
                                                                          ttCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTa                                                                                                                                        >  1:866889/1‑88 (MQ=255)
                                                                          ttCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTa                                                                                                                                        <  2:866889/88‑1 (MQ=255)
                                                                                              aCCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGcc                                                                                                                                   >  1:1566969/1‑73 (MQ=255)
                                                                                              aCCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGcc                                                                                                                                   <  2:1566969/73‑1 (MQ=255)
                                                                                              aCCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTca                                                      >  2:1011862/1‑150 (MQ=255)
                                                                                              aCCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTca                                                      >  2:68988/1‑150 (MQ=255)
                                                                                               ccTATTTTGTGAACCTACAAGGTGTAATTCGCCAGGCACTAAAAAGCCCGCAGCAGCGGGCTTTTTAGTGTCTg                                                                                                                                 >  2:1156610/1‑74 (MQ=18)
                                                                                               ccTATTTTGTGAACCTACAAGGTGTAATTCGCCAGGCACTAAAAAGCCCGCAGCAGCGGGCTTTTTAGTGTCTg                                                                                                                                 <  1:1156610/74‑1 (MQ=18)
                                                                                                cTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAg                                                                                                                   >  2:1037932/1‑87 (MQ=255)
                                                                                                cTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAg                                                                                                                   <  1:1037932/87‑1 (MQ=255)
                                                                                                cTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAAc                                                                    <  1:1213208/134‑1 (MQ=255)
                                                                                                cTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAAc                                                                    >  2:1213208/1‑134 (MQ=255)
                                                                                                 tATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACAt                                                   >  2:1617031/1‑150 (MQ=255)
                                                                                                            ccTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACga                                        <  1:1617031/150‑1 (MQ=255)
                                                                                                                    gtgtAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACg                                >  2:318663/1‑150 (MQ=255)
                                                                                                                       tAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACGAgg                             <  1:1011862/150‑1 (MQ=255)
                                                                                                                                 gACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACGAGGTGGCGCATAc                   <  1:318663/150‑1 (MQ=255)
                                                                                                                                     cTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACGAGGTGgcgc                       <  2:434520/142‑1 (MQ=255)
                                                                                                                                     cTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACGAGGTGgcgc                       >  1:434520/1‑142 (MQ=255)
                                                                                                                                      tAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACGAGGTGGCGCATACAGGTg              >  1:1169744/1‑150 (MQ=255)
                                                                                                                                      tAAAAAGCCCGCAGCAGCGGGCTTTTTAGTGTCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTca                                                      <  2:361125/110‑1 (MQ=38)
                                                                                                                                      tAAAAAGCCCGCAGCAGCGGGCTTTTTAGTGTCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTca                                                      >  1:361125/1‑110 (MQ=38)
                                                                                                                                        aaaaGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACGAGGTGGCGCATACAGGTGgc            >  1:852903/1‑150 (MQ=255)
                                                                                                                                             cccGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACGAGGTGGCGCATACAGGTGGCGCGCt       >  2:816915/1‑150 (MQ=255)
                                                                                                                                               cGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTcaca                                                    <  2:69291/103‑1 (MQ=255)
                                                                                                                                               cGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTcaca                                                    >  1:69291/1‑103 (MQ=255)
                                                                                                                                                  tgctgcGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACGAGGTGGCGCATACAGGTGGCGCGCTTTCca  <  2:811271/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
GTGAGTAGGGATTTCTCGGCTTTTCTGTGAAAAGCACCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACGAGGTGGCGCATACAGGTGGCGCGCTTTCCA  >  NC_002947/58601‑58896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑