Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,753,541 T→G 15.4% intergenic (+46/‑320) glnA → / → glnL glutamine synthetase/two‑component system sensor histidine kinase/phosphatase GlnL/GlnG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,753,5410TG15.4% 113.1 / 17.2 52intergenic (+46/‑320)glnA/glnLglutamine synthetase/two‑component system sensor histidine kinase/phosphatase GlnL/GlnG
Reads supporting (aligned to +/- strand):  ref base T (24/20);  new base G (4/4);  total (28/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.77e-01

CTTCATCGATGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTTCAGGTTAAACGTTTCACCAAGACTTTC  >  NC_002947/5753399‑5753687
                                                                                                                                              |                                                                                                                                                  
cTTCATCGATGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAgg                                                                                                                                             <  2:834309/150‑1 (MQ=255)
    aTCGATGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGt                                                                                                                                         >  2:863831/1‑150 (MQ=255)
    aTCGATGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCGAGGGAGGCCg                                                                                                                                          <  1:1476600/149‑1 (MQ=255)
    aTCGATGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCGAGGGAGGCCg                                                                                                                                          >  2:1476600/1‑149 (MQ=255)
    aTCGATGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCGAGGGAGGCCGt                                                                                                                                         <  1:54126/150‑1 (MQ=255)
    aTCGATGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTAATACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGt                                                                                                                                         >  2:1065921/1‑150 (MQ=255)
      cGATGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCGAGGGAGGCCGttt                                                                                                                                       <  2:547842/150‑1 (MQ=255)
        aTGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGttttt                                                                                                                                     <  2:30993/150‑1 (MQ=255)
           ccTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGtt                                                                                                                                  >  2:1273730/1‑150 (MQ=255)
             ttCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTc                                                                                                                                >  1:570196/1‑150 (MQ=255)
                 tCGAGCTGACGAGCGAAGAAGAAAGCAAGGCCACTCCATTCGTACCAGCGAGAGACTACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCCAAACGGCCTCCCTCGGGAGGCCGGTTTTGTTTCTgcg                                                                                                                            >  1:693057/1‑150 (MQ=255)
                    aGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCAc                                                                                                                         >  1:1590677/1‑150 (MQ=255)
                               taagaagaaATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAgc                                                                                                              <  1:759328/149‑1 (MQ=255)
                                aagaagaaATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTc                                                                                                                                <  1:684011/131‑1 (MQ=255)
                                aagaagaaATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTc                                                                                                                                >  2:684011/1‑131 (MQ=255)
                                   aagaaATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTTATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGcgccg                                                                                                          >  2:1358030/1‑150 (MQ=255)
                                   aagaaATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGcgccg                                                                                                          <  1:863831/150‑1 (MQ=255)
                                           aaGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCAc                                                                                                                         <  2:653210/127‑1 (MQ=255)
                                           aaGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCAc                                                                                                                         >  1:653210/1‑127 (MQ=255)
                                               tCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCGAGGGAGGCCGttt                                                                                                                                       <  1:287334/109‑1 (MQ=255)
                                               tCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCGAGGGAGGCCGttt                                                                                                                                       >  2:287334/1‑109 (MQ=255)
                                                  gTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCa                                                                                           <  2:394377/150‑1 (MQ=255)
                                                     ccTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGc                                                                                        >  2:298715/1‑150 (MQ=255)
                                                      cTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCt                                                                                       >  2:1594355/1‑150 (MQ=255)
                                                      cTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCt                                                                                       >  2:989232/1‑150 (MQ=255)
                                                                 cGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCt                                                                            <  2:1590677/150‑1 (MQ=255)
                                                                   cTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCCTTTTTGTTTCTGCGCACCt                                                                                                                       <  1:553369/105‑1 (MQ=255)
                                                                   cTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCCTTTTTGTTTCTGCGCACCt                                                                                                                       >  2:553369/1‑105 (MQ=255)
                                                                         tACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACg                                                                                         >  2:1379625/1‑129 (MQ=255)
                                                                         tACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACg                                                                                         <  1:1379625/129‑1 (MQ=255)
                                                                              gCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTg                                                                                                                     >  1:642099/1‑96 (MQ=255)
                                                                              gCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTg                                                                                                                     <  2:642099/96‑1 (MQ=255)
                                                                                    ctacAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACAcc                                                         >  1:193915/1‑150 (MQ=255)
                                                                                        aGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGc                                                     <  2:810884/150‑1 (MQ=255)
                                                                                             ctgatctgatTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGAGAGGCCGTTTTTGTTTCTGCGCAc                                                                                                                         <  1:1306534/77‑1 (MQ=255)
                                                                                             ctgatctgatTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGAGAGGCCGTTTTTGTTTCTGCGCAc                                                                                                                         >  2:1306534/1‑77 (MQ=255)
                                                                                              tgatctgatTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCaa                                               <  1:1065921/150‑1 (MQ=255)
                                                                                              tgatctgatTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCaa                                               <  2:570196/150‑1 (MQ=255)
                                                                                                atctgatTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAAtt                                             <  1:1273730/150‑1 (MQ=255)
                                                                                                 tctgatTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTg                                            >  1:810792/1‑150 (MQ=255)
                                                                                                     atTGGCGCCTCGCGCCGCTGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTcc                                        >  1:728592/1‑150 (MQ=255)
                                                                                                              tCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTgg                               >  2:807035/1‑150 (MQ=255)
                                                                                                                    cGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTTCAgg                         <  1:1358030/150‑1 (MQ=255)
                                                                                                                     gATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTTCAGGt                        >  2:1534061/1‑150 (MQ=255)
                                                                                                                       tGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTTCAg                          >  1:1221403/1‑146 (MQ=255)
                                                                                                                       tGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTTCAg                          <  2:1221403/146‑1 (MQ=255)
                                                                                                                            tGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTca                                                             >  2:406888/1‑106 (MQ=255)
                                                                                                                            tGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTca                                                             <  1:406888/106‑1 (MQ=255)
                                                                                                                               tCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGtt                             <  2:128567/135‑1 (MQ=255)
                                                                                                                               tCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGtt                             >  1:128567/1‑135 (MQ=255)
                                                                                                                                aaaaaCGGCCTCCCGAGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTTCAGGTTAAACGTTTCa             >  2:283352/2‑150 (MQ=255)
                                                                                                                                aaaaaCGGCCTCCCGAGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTTCAGGTTAAACGTTTCa             >  1:607169/2‑150 (MQ=255)
                                                                                                                                         ctccctcGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTTCAGGTTAAACGTTTCACCAAGACtt    >  1:502943/1‑150 (MQ=255)
                                                                                                                                          tccctcGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTTCAGGTTAAACGTTTCACCAAGACttt   >  1:501512/1‑150 (MQ=255)
                                                                                                                                           ccctcGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTTCAGGTTAAACGTTTCACCAAGACTTTc  >  1:1376576/1‑150 (MQ=255)
                                                                                                                                           ccctcGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTTCAGGTTAAACGTTTCACCAAGACTTTc  <  1:807035/150‑1 (MQ=255)
                                                                                                                                            cctcGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAAtt                                                               <  2:1351256/88‑1 (MQ=255)
                                                                                                                                            cctcGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAAtt                                                               >  1:1351256/1‑88 (MQ=255)
                                                                                                                                             ctcGGGAGGCCGTTTTTGTTTCTGCGCACCTTTCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTTCAGGTTAAACGTTTCACCAAGa       <  2:751291/143‑1 (MQ=255)
                                                                                                                                             ctcGGGAGGCCGTTTTTGTTTCTGCGCACCTTTCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTTCAGGTTAAACGTTTCACCAAGa       >  1:751291/1‑143 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
CTTCATCGATGCCTTCATCGAGCTGAAGAGCGAAGAAGAAATCAAGGTCCGTACCTTCGTACACCCGCTGGAATACGAGCTGTACTACAGCTGCTGATCTGATTGGCGCCTCGCGCCGATGACATGATCAAAACGGCCTCCCTCGGGAGGCCGTTTTTGTTTCTGCGCACCTTGCAGAAGCGCCGCTGATTCGCCTGTCACGCTATGCTCTATCTTGGTGCATTAATTCACACCATGCCCTCAATTGCTCCCCAATCTGGTTCAGGTTAAACGTTTCACCAAGACTTTC  >  NC_002947/5753399‑5753687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑