Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,798,249 C→T 9.1% intergenic (‑294/+80) gltB ← / ← PP_5077 L‑glutamate synthase subunit alpha/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,798,2490CT 9.1% 146.1 / 5.4 55intergenic (‑294/+80)gltB/PP_5077L‑glutamate synthase subunit alpha/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (28/22);  new base T (2/3);  total (30/25)
Fisher's exact test for biased strand distribution p-value = 6.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.77e-01

TTTCATGTCTCGCCATTGAAAATTTATGTCGCAATTTTGAATGTTTTTGCGCCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTACGTGGCCAAGGTTTACCAGCCTGG  >  NC_002947/5798100‑5798395
                                                                                                                                                     |                                                                                                                                                  
tttCATGTCTCGCCATTGAAAATTTATGTCGCAATTTTGAATGTTTTTGCGCCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGcc                                                                                                                                                    <  1:1405788/150‑1 (MQ=255)
       tctcGCCATTGAATATTTATGTCGCAATTTTGAATGTTTTTGCGCCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCg                                                                                                                                             >  1:799919/1‑150 (MQ=255)
         tcGCCATTGAAAATTTATGTCGCAATTTTGAATGTTTTTGCGCCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCggg                                                                                                                                           >  2:1479717/1‑150 (MQ=255)
                 gAAAATTTATGTCGCAATTTTGAATGTTTTTGCGCCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGcagcag                                                                                                                                   >  2:1122670/1‑150 (MQ=255)
                  aaaaTTTATGTCGCAATTTTGAATGTTTTTTTTTTTTTTTCCTTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTTGGGCGTTGCAgg                                                                                                                                  >  2:1582349/1‑150 (MQ=255)
                      tttATGTCGCAATTTTGAATGTTTTTGCGCCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGGCTTCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCTGCGCCCGAGCGCGGCAGGCttt                                                                                                                              <  1:472368/150‑3 (MQ=255)
                               cAATTTTGAATGTTTTTGCGCCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAACCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCt                                                                                                                     >  2:113563/1‑150 (MQ=255)
                                aaTTTTGAATGTTTTTGCGCCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTa                                                                                                                    <  1:1122670/150‑1 (MQ=255)
                                        aTGTTTTTGCGCCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGc                                                                                                            <  1:1222878/150‑1 (MQ=255)
                                            ttttGCGCCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAg                                                                                                        <  2:1185970/150‑1 (MQ=255)
                                                   ccATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGGCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTgg                                                                                                 <  1:1582349/150‑1 (MQ=255)
                                                          ccGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCTGCGCCCGAGCGCGGCAGGCtttt                                                                                                                             >  2:1616866/1‑112 (MQ=38)
                                                          ccGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCTGCGCCCGAGCGCGGCAGGCtttt                                                                                                                             <  1:1616866/115‑4 (MQ=38)
                                                          ccGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGcggcgg                                                                                          >  2:1537099/1‑150 (MQ=255)
                                                                  gCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTGGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCACAGCAGGCTAGTCTAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATgc                                                                                  <  1:610857/150‑1 (MQ=255)
                                                                    ggCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATgccg                                                                                >  1:1502719/1‑150 (MQ=255)
                                                                            tCGGGTCTGAAGCGTTTCAGCCATAACTGCACATACGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAg                                                                        <  2:1502719/150‑1 (MQ=255)
                                                                            tCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAg                                                                        >  1:1242737/1‑150 (MQ=255)
                                                                                 tCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATc                                                                   >  2:1338936/1‑150 (MQ=255)
                                                                                 tCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATc                                                                   <  1:1160535/150‑1 (MQ=255)
                                                                                   tGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCgcagca                                                                                                                                    >  2:1231440/1‑83 (MQ=255)
                                                                                   tGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCgcagca                                                                                                                                    <  1:1231440/83‑1 (MQ=255)
                                                                                      aGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCg                                                               <  1:411294/149‑1 (MQ=255)
                                                                                      aGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCg                                                               >  2:411294/1‑149 (MQ=255)
                                                                                      aGCGTTTCAGCCATGACTGCAACTAAGAGCTTGTGGCCGGCAGTCCGGGACAGAAAAACCCACCAAGCCCGGGCGCCGCAGGCTAGTCGAAAGCCAGAATTCGCTTCGCTACTGGGCGGCGGGGCGACGCCCCCCGCAAGGTATCAGCgg                                                              >  2:101039/1‑150 (MQ=255)
                                                                                          tttCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAAtt                                                                                                               >  2:820711/1‑97 (MQ=255)
                                                                                          tttCAGCCATGACTGCAACTAAGGGCTTGTGGCAGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAAtt                                                                                                               <  1:820711/97‑1 (MQ=255)
                                                                                            tCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTg                                                        <  1:1479717/150‑1 (MQ=255)
                                                                                            tCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTg                                                        <  2:1242737/150‑1 (MQ=255)
                                                                                                ccATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCa                                                    >  1:1511481/1‑150 (MQ=255)
                                                                                                      cTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCtt                                              <  1:1338936/150‑1 (MQ=255)
                                                                                                       tGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGGCAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCggg                                                                                         <  1:796777/106‑1 (MQ=255)
                                                                                                       tGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCggg                                                                                         >  2:584825/1‑106 (MQ=255)
                                                                                                       tGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCggg                                                                                         <  1:584825/106‑1 (MQ=255)
                                                                                                       tGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCggg                                                                                         >  2:796777/1‑106 (MQ=255)
                                                                                                            cTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTggc                                        <  2:1511481/150‑2 (MQ=255)
                                                                                                             tAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAAtt                                                                                                               <  1:1332402/78‑1 (MQ=255)
                                                                                                             tAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAAtt                                                                                                               >  2:1332402/1‑78 (MQ=255)
                                                                                                                gggCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCggg                                                                                         >  1:184930/1‑97 (MQ=255)
                                                                                                                gggCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCggg                                                                                         <  2:184930/97‑1 (MQ=255)
                                                                                                                   cTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGcc                                 >  1:388854/1‑150 (MQ=255)
                                                                                                                    ttGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCg                                >  2:1409154/1‑150 (MQ=255)
                                                                                                                     tgtgGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGa                               >  2:73338/1‑150 (MQ=255)
                                                                                                                           cGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTAc                         >  2:254134/1‑150 (MQ=255)
                                                                                                                              cAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTACGTg                      <  1:1409154/150‑1 (MQ=255)
                                                                                                                                 tCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTACGTGGcc                   >  2:1435716/1‑150 (MQ=255)
                                                                                                                                 tCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTACGTGGcc                   >  1:824937/1‑150 (MQ=255)
                                                                                                                                        cAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTACGTGGCCAAGGttt            >  2:1460958/1‑150 (MQ=255)
                                                                                                                                          gAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTACGTGGCCAAGGTTTAc          <  1:1537099/150‑1 (MQ=255)
                                                                                                                                              aGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGACCGGAAGGTATCAGCACGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTACGTGGCCAAGGTTTACCAGc      <  1:1435716/150‑1 (MQ=255)
                                                                                                                                                ccTGCTGCGCCCGAGCGCGGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCttgttg                                          <  1:188774/112‑1 (MQ=255)
                                                                                                                                                ccTGCTGCGCCCGAGCGCGGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCttgttg                                          >  2:188774/1‑112 (MQ=255)
                                                                                                                                                 cTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTACGTGGCCAAGGTTTACCAGCCTg   >  1:749011/1‑150 (MQ=255)
                                                                                                                                                  tgctgcGCCCGAGCGCGGCAGGCTTTTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTACGTGGCCAAGGTTTACCAGCCTgg  >  2:1356171/1‑150 (MQ=255)
                                                                                                                                                  tGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGc                                                                <  2:1640065/88‑1 (MQ=255)
                                                                                                                                                  tGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGc                                                                >  1:1640065/1‑88 (MQ=255)
                                                                                                                                                  tGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTACGTGGCCAAGGTTTACCAGCCTgg  >  1:1072160/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
TTTCATGTCTCGCCATTGAAAATTTATGTCGCAATTTTGAATGTTTTTGCGCCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTACGTGGCCAAGGTTTACCAGCCTGG  >  NC_002947/5798100‑5798395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑