Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,798,254 T→C 12.2% intergenic (‑299/+75) gltB ← / ← PP_5077 L‑glutamate synthase subunit alpha/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,798,2540TC12.2% 141.9 / 8.3 56intergenic (‑299/+75)gltB/PP_5077L‑glutamate synthase subunit alpha/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (27/22);  new base C (4/3);  total (31/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

TCTCGCCATTGAAAATTTATGTCGCAATTTTGAATGTTTTTGCGCCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTACGTGGCCAAGGTTTACCAGCCTGG  >  NC_002947/5798107‑5798395
                                                                                                                                                   |                                                                                                                                             
tctcGCCATTGAATATTTATGTCGCAATTTTGAATGTTTTTGCGCCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCg                                                                                                                                             >  1:799919/1‑150 (MQ=255)
  tcGCCATTGAAAATTTATGTCGCAATTTTGAATGTTTTTGCGCCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCggg                                                                                                                                           >  2:1479717/1‑150 (MQ=255)
          gAAAATTTATGTCGCAATTTTGAATGTTTTTGCGCCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGcagcag                                                                                                                                   >  2:1122670/1‑150 (MQ=255)
           aaaaTTTATGTCGCAATTTTGAATGTTTTTTTTTTTTTTTCCTTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTTGGGCGTTGCAgg                                                                                                                                  >  2:1582349/1‑150 (MQ=255)
               tttATGTCGCAATTTTGAATGTTTTTGCGCCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGGCTTCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCTGCGCCCGAGCGCGGCAGGCttt                                                                                                                              <  1:472368/150‑3 (MQ=255)
                        cAATTTTGAATGTTTTTGCGCCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAACCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCt                                                                                                                     >  2:113563/1‑150 (MQ=255)
                         aaTTTTGAATGTTTTTGCGCCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTa                                                                                                                    <  1:1122670/150‑1 (MQ=255)
                                 aTGTTTTTGCGCCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGc                                                                                                            <  1:1222878/150‑1 (MQ=255)
                                     ttttGCGCCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAg                                                                                                        <  2:1185970/150‑1 (MQ=255)
                                            ccATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGGCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTgg                                                                                                 <  1:1582349/150‑1 (MQ=255)
                                                   ccGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCTGCGCCCGAGCGCGGCAGGCtttt                                                                                                                             >  2:1616866/1‑112 (MQ=38)
                                                   ccGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCTGCGCCCGAGCGCGGCAGGCtttt                                                                                                                             <  1:1616866/115‑4 (MQ=38)
                                                   ccGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGcggcgg                                                                                          >  2:1537099/1‑150 (MQ=255)
                                                           gCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTGGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCACAGCAGGCTAGTCTAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATgc                                                                                  <  1:610857/150‑1 (MQ=255)
                                                             ggCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATgccg                                                                                >  1:1502719/1‑150 (MQ=255)
                                                                     tCGGGTCTGAAGCGTTTCAGCCATAACTGCACATACGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAg                                                                        <  2:1502719/150‑1 (MQ=255)
                                                                     tCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAg                                                                        >  1:1242737/1‑150 (MQ=255)
                                                                          tCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATc                                                                   <  1:1160535/150‑1 (MQ=255)
                                                                          tCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATc                                                                   >  2:1338936/1‑150 (MQ=255)
                                                                            tGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCgcagca                                                                                                                                    >  2:1231440/1‑83 (MQ=255)
                                                                            tGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCgcagca                                                                                                                                    <  1:1231440/83‑1 (MQ=255)
                                                                               aGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCg                                                               >  2:411294/1‑149 (MQ=255)
                                                                               aGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCg                                                               <  1:411294/149‑1 (MQ=255)
                                                                               aGCGTTTCAGCCATGACTGCAACTAAGAGCTTGTGGCCGGCAGTCCGGGACAGAAAAACCCACCAAGCCCGGGCGCCGCAGGCTAGTCGAAAGCCAGAATTCGCTTCGCTACTGGGCGGCGGGGCGACGCCCCCCGCAAGGTATCAGCgg                                                              >  2:101039/1‑150 (MQ=255)
                                                                                   tttCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAAtt                                                                                                               >  2:820711/1‑97 (MQ=255)
                                                                                   tttCAGCCATGACTGCAACTAAGGGCTTGTGGCAGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAAtt                                                                                                               <  1:820711/97‑1 (MQ=255)
                                                                                     tCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTg                                                        <  1:1479717/150‑1 (MQ=255)
                                                                                     tCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTg                                                        <  2:1242737/150‑1 (MQ=255)
                                                                                         ccATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCa                                                    >  1:1511481/1‑150 (MQ=255)
                                                                                               cTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCtt                                              <  1:1338936/150‑1 (MQ=255)
                                                                                                tGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGGCAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCggg                                                                                         <  1:796777/106‑1 (MQ=255)
                                                                                                tGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCggg                                                                                         >  2:584825/1‑106 (MQ=255)
                                                                                                tGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCggg                                                                                         <  1:584825/106‑1 (MQ=255)
                                                                                                tGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCggg                                                                                         >  2:796777/1‑106 (MQ=255)
                                                                                                     cTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTggc                                        <  2:1511481/150‑2 (MQ=255)
                                                                                                      tAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAAtt                                                                                                               <  1:1332402/78‑1 (MQ=255)
                                                                                                      tAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAAtt                                                                                                               >  2:1332402/1‑78 (MQ=255)
                                                                                                         gggCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCggg                                                                                         >  1:184930/1‑97 (MQ=255)
                                                                                                         gggCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCggg                                                                                         <  2:184930/97‑1 (MQ=255)
                                                                                                            cTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGcc                                 >  1:388854/1‑150 (MQ=255)
                                                                                                             ttGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCg                                >  2:1409154/1‑150 (MQ=255)
                                                                                                              tgtgGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGa                               >  2:73338/1‑150 (MQ=255)
                                                                                                                    cGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTAc                         >  2:254134/1‑150 (MQ=255)
                                                                                                                       cAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTACGTg                      <  1:1409154/150‑1 (MQ=255)
                                                                                                                          tCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTACGTGGcc                   >  2:1435716/1‑150 (MQ=255)
                                                                                                                          tCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTACGTGGcc                   >  1:824937/1‑150 (MQ=255)
                                                                                                                                 cAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTACGTGGCCAAGGttt            >  2:1460958/1‑150 (MQ=255)
                                                                                                                                   gAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTACGTGGCCAAGGTTTAc          <  1:1537099/150‑1 (MQ=255)
                                                                                                                                       aGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGACCGGAAGGTATCAGCACGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTACGTGGCCAAGGTTTACCAGc      <  1:1435716/150‑1 (MQ=255)
                                                                                                                                         ccTGCTGCGCCCGAGCGCGGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCttgttg                                          <  1:188774/112‑1 (MQ=255)
                                                                                                                                         ccTGCTGCGCCCGAGCGCGGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCttgttg                                          >  2:188774/1‑112 (MQ=255)
                                                                                                                                          cTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTACGTGGCCAAGGTTTACCAGCCTg   >  1:749011/1‑150 (MQ=255)
                                                                                                                                           tgctgcGCCCGAGCGCGGCAGGCTTTTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTACGTGGCCAAGGTTTACCAGCCTgg  >  2:1356171/1‑150 (MQ=255)
                                                                                                                                           tGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGc                                                                <  2:1640065/88‑1 (MQ=255)
                                                                                                                                           tGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGc                                                                >  1:1640065/1‑88 (MQ=255)
                                                                                                                                           tGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTACGTGGCCAAGGTTTACCAGCCTgg  >  1:1072160/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                             
TCTCGCCATTGAAAATTTATGTCGCAATTTTGAATGTTTTTGCGCCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTACGTGGCCAAGGTTTACCAGCCTGG  >  NC_002947/5798107‑5798395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑