Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,798,262 A→G 12.3% intergenic (‑307/+67) gltB ← / ← PP_5077 L‑glutamate synthase subunit alpha/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,798,2620AG12.3% 122.9 / 8.8 51intergenic (‑307/+67)gltB/PP_5077L‑glutamate synthase subunit alpha/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (22/21);  new base G (3/3);  total (27/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.56e-01

GAAAATTTATGTCGCAATTTTGAATGTTTTTGCGCCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTACGTGGCCAAGGTTTACCAGCCTGG  >  NC_002947/5798117‑5798395
                                                                                                                                                 |                                                                                                                                     
gAAAATTTATGTCGCAATTTTGAATGTTTTTGCGCCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGcagcag                                                                                                                                   >  2:1122670/1‑150 (MQ=255)
 aaaaTTTATGTCGCAATTTTGAATGTTTTTTTTTTTTTTTCCTTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTTGGGCGTTGCAgg                                                                                                                                  >  2:1582349/1‑150 (MQ=255)
     tttATGTCGCAATTTTGAATGTTTTTGCGCCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGGCTTCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCTGCGCCCGAGCGCGGCAGGCttt                                                                                                                              <  1:472368/150‑3 (MQ=255)
              cAATTTTGAATGTTTTTGCGCCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAACCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCt                                                                                                                     >  2:113563/1‑150 (MQ=255)
               aaTTTTGAATGTTTTTGCGCCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTa                                                                                                                    <  1:1122670/150‑1 (MQ=255)
                       aTGTTTTTGCGCCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGc                                                                                                            <  1:1222878/150‑1 (MQ=255)
                           ttttGCGCCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAg                                                                                                        <  2:1185970/150‑1 (MQ=255)
                                  ccATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGGCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTgg                                                                                                 <  1:1582349/150‑1 (MQ=255)
                                         ccGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCTGCGCCCGAGCGCGGCAGGCtttt                                                                                                                             >  2:1616866/1‑112 (MQ=38)
                                         ccGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCTGCGCCCGAGCGCGGCAGGCtttt                                                                                                                             <  1:1616866/115‑4 (MQ=38)
                                         ccGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGcggcgg                                                                                          >  2:1537099/1‑150 (MQ=255)
                                                 gCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTGGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCACAGCAGGCTAGTCTAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATgc                                                                                  <  1:610857/150‑1 (MQ=255)
                                                   ggCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATgccg                                                                                >  1:1502719/1‑150 (MQ=255)
                                                           tCGGGTCTGAAGCGTTTCAGCCATAACTGCACATACGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAg                                                                        <  2:1502719/150‑1 (MQ=255)
                                                           tCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAg                                                                        >  1:1242737/1‑150 (MQ=255)
                                                                tCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATc                                                                   >  2:1338936/1‑150 (MQ=255)
                                                                tCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATc                                                                   <  1:1160535/150‑1 (MQ=255)
                                                                  tGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCgcagca                                                                                                                                    >  2:1231440/1‑83 (MQ=255)
                                                                  tGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCgcagca                                                                                                                                    <  1:1231440/83‑1 (MQ=255)
                                                                     aGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCg                                                               >  2:411294/1‑149 (MQ=255)
                                                                     aGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCg                                                               <  1:411294/149‑1 (MQ=255)
                                                                     aGCGTTTCAGCCATGACTGCAACTAAGAGCTTGTGGCCGGCAGTCCGGGACAGAAAAACCCACCAAGCCCGGGCGCCGCAGGCTAGTCGAAAGCCAGAATTCGCTTCGCTACTGGGCGGCGGGGCGACGCCCCCCGCAAGGTATCAGCgg                                                              >  2:101039/1‑150 (MQ=255)
                                                                         tttCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAAtt                                                                                                               >  2:820711/1‑97 (MQ=255)
                                                                         tttCAGCCATGACTGCAACTAAGGGCTTGTGGCAGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAAtt                                                                                                               <  1:820711/97‑1 (MQ=255)
                                                                           tCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTg                                                        <  1:1479717/150‑1 (MQ=255)
                                                                           tCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTg                                                        <  2:1242737/150‑1 (MQ=255)
                                                                               ccATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCa                                                    >  1:1511481/1‑150 (MQ=255)
                                                                                     cTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCtt                                              <  1:1338936/150‑1 (MQ=255)
                                                                                      tGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGGCAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCggg                                                                                         <  1:796777/106‑1 (MQ=255)
                                                                                      tGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCggg                                                                                         <  1:584825/106‑1 (MQ=255)
                                                                                      tGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCggg                                                                                         >  2:584825/1‑106 (MQ=255)
                                                                                      tGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCggg                                                                                         >  2:796777/1‑106 (MQ=255)
                                                                                           cTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTggc                                        <  2:1511481/150‑2 (MQ=255)
                                                                                            tAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAAtt                                                                                                               <  1:1332402/78‑1 (MQ=255)
                                                                                            tAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAAtt                                                                                                               >  2:1332402/1‑78 (MQ=255)
                                                                                               gggCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCggg                                                                                         >  1:184930/1‑97 (MQ=255)
                                                                                               gggCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCggg                                                                                         <  2:184930/97‑1 (MQ=255)
                                                                                                  cTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGcc                                 >  1:388854/1‑150 (MQ=255)
                                                                                                   ttGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCg                                >  2:1409154/1‑150 (MQ=255)
                                                                                                    tgtgGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGa                               >  2:73338/1‑150 (MQ=255)
                                                                                                          cGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTAc                         >  2:254134/1‑150 (MQ=255)
                                                                                                             cAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTACGTg                      <  1:1409154/150‑1 (MQ=255)
                                                                                                                tCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTACGTGGcc                   >  2:1435716/1‑150 (MQ=255)
                                                                                                                tCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTACGTGGcc                   >  1:824937/1‑150 (MQ=255)
                                                                                                                       cAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTACGTGGCCAAGGttt            >  2:1460958/1‑150 (MQ=255)
                                                                                                                         gAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTACGTGGCCAAGGTTTAc          <  1:1537099/150‑1 (MQ=255)
                                                                                                                             aGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGACCGGAAGGTATCAGCACGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTACGTGGCCAAGGTTTACCAGc      <  1:1435716/150‑1 (MQ=255)
                                                                                                                               ccTGCTGCGCCCGAGCGCGGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCttgttg                                          <  1:188774/112‑1 (MQ=255)
                                                                                                                               ccTGCTGCGCCCGAGCGCGGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCttgttg                                          >  2:188774/1‑112 (MQ=255)
                                                                                                                                cTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTACGTGGCCAAGGTTTACCAGCCTg   >  1:749011/1‑150 (MQ=255)
                                                                                                                                 tgctgcGCCCGAGCGCGGCAGGCTTTTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTACGTGGCCAAGGTTTACCAGCCTgg  >  2:1356171/1‑150 (MQ=255)
                                                                                                                                 tGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGc                                                                <  2:1640065/88‑1 (MQ=255)
                                                                                                                                 tGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGc                                                                >  1:1640065/1‑88 (MQ=255)
                                                                                                                                 tGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTACGTGGCCAAGGTTTACCAGCCTgg  >  1:1072160/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                     
GAAAATTTATGTCGCAATTTTGAATGTTTTTGCGCCATGCGCCGTGATAGCGGCTTGGCTCGAGTCTGAAGCGTTTCAGCCATGACTGCAACTAAGGGCTTGTGGCCGGCAGTCCGGGACAGAAAAGCCTGCCGCGCTCGGGCGCAGCAGGCTAGTCGAAAGCTAGAATTCGCTTAGCTACTGGGCGGCGGGGTGATGCCGCCCGGAAGGTATCAGCGGGCCGTGGCCAGCTCTTGTTGGACGCTGCCGACGGTACGTGGCCAAGGTTTACCAGCCTGG  >  NC_002947/5798117‑5798395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑