Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,830,754 G→T 5.6% A482A (GCC→GCA)  ftsY ← signal recognition particle protein translocation receptor GTPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,830,7540GT 5.6% 231.7 / 2.3 71A482A (GCC→GCA) ftsYsignal recognition particle protein translocation receptor GTPase
Reads supporting (aligned to +/- strand):  ref base G (32/35);  new base T (2/2);  total (34/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.16e-01

CGGTGACGAACAGGAATTCGCCCCGGCGCGCCCGGAAACTCAGCTCATGCAAACCCACATGACCGTTGGGATAGCGCTTGGCAACCTGCTCGAATCGGATCATGGTCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCTTGGTCAGGGCCAGGCCGGTCAGCTCGACGCTC  >  NC_002947/5830610‑5830901
                                                                                                                                                |                                                                                                                                                   
cGGTGACGAACAGGAATTCGCCCCGGCGCGCCCGGAAACTCAGCTCATGCAAACCCACATGACCGTTGGGATAGCGCTTGGCAACCTGCTCGAATCGGATCATGGTCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTc                                                                                                                                                >  1:517126/1‑150 (MQ=255)
cGGTGACGAACAGGAATTCGCCCCGGCGCGCCCGGAAACTCAGCTCATGCAAACCCACATGACCGTTGGGATAGCGCTTGGCAACCTGCTCGAATCGGATCATGGTCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTc                                                                                                                                                >  1:929844/1‑150 (MQ=255)
 ggTGACGAACAGGAATTCGCCCCGGCGCGCCCGGAAACTCAGCTCATGCAAACCCACATGACCGTTGGGATAGCGCTTGGCAACCTGCTCGAATCGGATCATGGTCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCg                                                                                                                                               >  1:297863/1‑150 (MQ=255)
    gACGAACAGGAATTCGCCCCGGCGCGCCCGGAAACTCAGCTCATGCAAACCCACATGACCGTTGGGATAGCGCTTGGCAACCTGCTCGAATCGGATCATGGTCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAg                                                                                                                                            <  2:297863/150‑1 (MQ=255)
    gACGAACAGGAATTCGCCCCGGCGCGCCCGGAAACCCAGCTCAAGCAAACCCACATGACCGTTGGGATAGCGCTTGGCAACCTGCTCGAATCGGATCATGGTCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAg                                                                                                                                            <  1:1328177/150‑1 (MQ=255)
       gAACAGGAATTCGCCCCGGCGCGCCCGGAAACTCAGCTCATGCAAACCCACATGACCGTTGGGATAGCGCTTGGCAACCTGCTCGAATCGGATCATGGTCAATCTCGCTCGGCTAACAGAGCCTTGACGAACGGCTCGGCTTCGAAg                                                                                                                                            <  2:440871/147‑1 (MQ=255)
       gAACAGGAATTCGCCCCGGCGCGCCCGGAAACTCAGCTCATGCAAACCCACATGACCGTTGGGATAGCGCTTGGCAACCTGCTCGAATCGGATCATGGTCAATCTCGCTCGGCTAACAGAGCCTTGACGAACGGCTCGGCTTCGAAg                                                                                                                                            >  1:440871/1‑147 (MQ=255)
       gAACAGGAATTCGCCCCGGCGCGCCCGGAAACTCAGCTCATGCAAACCCACATGACCGTTGGGATAGCGCTTGGCAACCTGCTCGAATCGGATCATGGTCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTa                                                                                                                                         >  1:137873/1‑150 (MQ=255)
       gAACAGGAATTCGCCCCGGCGCGCCCGGAAACTCAGCTCATGCAAACCCACATGACCGTTGGGATAGCGCTTGGCAACCTGCTCGAATCGGATCATGGTCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTa                                                                                                                                         <  2:599348/150‑1 (MQ=255)
        aaCAGGAATTCGCCCCGGCGCGCCCGGAAACTCAGCTCATGCAAACCCACATGACCGTTGGGATAGCGCTTGGCAACCTGCTCGAATCGGATCATGGTCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGGTCGGCTTCGAAGGTAc                                                                                                                                        <  2:373389/150‑1 (MQ=255)
        aaCAGGAATTCGCCCCGGCGCGCCCGGAAACTCAGCTCATGCAAACCCACATGACCGTTGGGATAGCGCTTGGCAACCTGCTCGAATCGGATCATGGTCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTAc                                                                                                                                        >  1:291224/1‑150 (MQ=255)
         aCAGGAATTCGCCCCGGCGCGCCCGGAAACTCAGCTCATGCAAACCCACATGACCGTTGGGATAGCGCTTGGCAACCTGCTCGAATCGGATCATGGTCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAg                                                                                                                                            >  2:489092/1‑145 (MQ=255)
         aCAGGAATTCGCCCCGGCGCGCCCGGAAACTCAGCTCATGCAAACCCACATGACCGTTGGGATAGCGCTTGGCAACCTGCTCGAATCGGATCATGGTCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAg                                                                                                                                            <  1:489092/145‑1 (MQ=255)
          cAGGAATTCGCCCCGGCGCGCCCGGAAACTCAGCTCATGCAAACCCACATGACCGTTGGGATAGCGCTTGGCAACCTGCTCGAATCGGATCATGGTCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGc                                                                                                                                      <  2:517126/150‑1 (MQ=255)
          cAGGAATTCGCCCCGGCGCGCCCGGAAACTCAGCTCATGCAAACCCACATGACCGTTGGGATAGCGCTTGGCAACCTGCTCGAATCGGATCATGGTCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGc                                                                                                                                      <  1:764271/150‑1 (MQ=255)
           ttgAAGTCGCCCCGGCGCGCCCGGAAACTCAGCTCATGCAAACCCACATGACCGTTGGGATAGCGCGTGGCAACCGGCTCGAATCGGATCATGGTCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGCa                                                                                                                                     <  1:361204/148‑1 (MQ=255)
                    ccccGGCGCGCCCGGAAACTCAGCTCATGCAAACCCACATGACCGTTGGGATAGCGCTTGGCAACCTGCTCGAATCGGATCATGGTCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGa                                                                                                                            >  2:943495/1‑150 (MQ=255)
                     cccGGCGCGCCCGGAAACTCAGCTCATGCAAACCCACATGACCGTTGGGATAGCGCTTGGCAACCTGCTCGAATCGGATCATGGTCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGAt                                                                                                                           <  1:234392/150‑1 (MQ=255)
                       cGGCGCGCCCGGAAACTCAGCTCATGCAAACCCACATGACCGTTGGGATAGCGCTTGGCAACCTGCTCGAATCGGATCATGGTCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGc                                                                                                                         <  1:657624/150‑1 (MQ=255)
                                     aCTCAGCTCATGCAAACCCACATGACCGTTGGGATAGCGCTTGGCAACCTGCTCGAATCGGATCATGGTCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCTGCTTCGAAGGTACGCAGgtc                                                                                                                                 <  1:1443454/128‑1 (MQ=255)
                                     aCTCAGCTCATGCAAACCCACATGACCGTTGGGATAGCGCTTGGCAACCTGCTCGAATCGGATCATGGTCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCTGCTTCGAAGGTACGCAGgtc                                                                                                                                 <  1:1443817/128‑1 (MQ=255)
                                     aCTCAGCTCATGCAAACCCACATGACCGTTGGGATAGCGCTTGGCAACCTGCTCGAATCGGATCATGGTCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCTGCTTCGAAGGTACGCAGgtc                                                                                                                                 >  2:1443817/1‑128 (MQ=255)
                                     aCTCAGCTCATGCAAACCCACATGACCGTTGGGATAGCGCTTGGCAACCTGCTCGAATCGGATCATGGTCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCTGCTTCGAAGGTACGCAGgtc                                                                                                                                 >  2:1443454/1‑128 (MQ=255)
                                       tCAGCTCATGCAAACCCACATGACCGTTGGGATAGCGCTTGGCAACCTGCTCGAATCGGATCATGGTCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGAt                                                                                                         <  1:353709/150‑1 (MQ=255)
                                       tCAGCTCATGCAAACCCACATGACCGTTGGGATAGCGCTTGGCAACCTGCTCGAATCGGATCATGGTCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGAt                                                                                                         >  1:666480/1‑150 (MQ=255)
                                           cTCATGCAAACCCACATGACCGTTGGGATAGCGCTTGGCAACCTGCTCGAATCGGATCATGGTCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGaaa                                                                                                     <  2:1448315/150‑1 (MQ=255)
                                            tCATGCAAACCCACATGACCGTTGGGATAGCGCTTGGCAACCTGCTCGAATCGGATCATGGTCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAAc                                                                                                    >  2:1184204/1‑150 (MQ=255)
                                              aTGCAAACCCACATGACCGTTGGGATAGCGCTTGGCAACCTGCTCGAATCGGATCATGGTCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACgg                                                                                                  >  2:1215850/1‑150 (MQ=255)
                                               tGCAAACCCACATGACCGTTGGGATAGCGCTTGGCAACCTGCTCGAATCGGATCATGGTCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGa                                                                                                 <  1:1172699/150‑1 (MQ=255)
                                                gCAAACCCACATGACCGTTGGGATAGCGCTTGGCAACCTGCTCGAATCGGATCATGGTCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGAt                                                                                                <  1:618981/150‑1 (MQ=255)
                                                        acaTGACCGTTGGGATAGCGCTTGGCAACCTGCTCGAATCGGATCATGGTCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGt                                                                                                                                          <  1:186242/100‑1 (MQ=255)
                                                        acaTGACCGTTGGGATAGCGCTTGGCAACCTGCTCGAATCGGATCATGGTCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGt                                                                                                                                          >  2:186242/1‑100 (MQ=255)
                                                        acaTGACCGTTGGGATAGCGCTTGGCAACCTGCTCGAATCGGATCATGGTCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGt                                                                                        <  2:666480/150‑1 (MQ=255)
                                                               cGTTGGGATAGCGCTTGGCAACCTGCTCGAATCGGATCATGGTCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCCCCGACGCCGATGAACCGGATCGGGATCTTGAACTg                                                                                 >  2:125511/1‑150 (MQ=255)
                                                               cGTTGGGATAGCGCTTGGCAACCTGCTCGAATCGGATCATGGTCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTg                                                                                 >  1:157038/1‑150 (MQ=255)
                                                                gTTGGGATAGCGCTTGGCAACCTGCTCGAATCGGATCATGGTCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGc                                                                                >  1:844477/1‑150 (MQ=255)
                                                                             ttGGCAACCTGCTCGAATCGGATCATGGTCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGa                                                                   >  2:274844/1‑150 (MQ=255)
                                                                              tGGCAACCTGCTCGAATCGGATCATGGTCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGaa                                                                  <  1:558948/150‑1 (MQ=255)
                                                                              tGGCAACCTGCTCGAATCGGATCATGGTCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGaa                                                                  <  2:291224/150‑1 (MQ=255)
                                                                               ggCAACCTGCTCGAATCGGATCATGGTCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACTGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAg                                                                 <  2:929844/150‑1 (MQ=255)
                                                                                 cAACCTGCTCGAATCGGATCATGGTCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGAt                                                               <  1:1499528/150‑1 (MQ=255)
                                                                                  aaCCTGCTCGAATCGGATCATGGTCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATc                                                              >  1:970334/1‑150 (MQ=255)
                                                                                     cTGCTCGAATCGGATCATGGTCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGa                                                                                                 >  1:1579522/1‑112 (MQ=255)
                                                                                     cTGCTCGAATCGGATCATGGTCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGa                                                                                                 <  2:1579522/112‑1 (MQ=255)
                                                                                     cTGCTCGAATCGGATCATGGTCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCAcc                                                           >  1:482750/1‑150 (MQ=255)
                                                                                      tGCTCGAATCGGATCATGGTCAATCGCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCAccc                                                          >  2:256086/1‑150 (MQ=255)
                                                                                         tCGAATCGGATCATGGTCAATCTCGCTCGGCGAACAGAGCCTTGACGAGCTGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCATCCCGc                                                       >  2:1559406/1‑150 (MQ=255)
                                                                                           gAATCGGATCATGGTCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTAGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGGTATTAACCTGCTTGGCCAGGGCGAAGATCACCCCGccc                                                     >  2:125947/1‑150 (MQ=255)
                                                                                            aaTCGGATCATGGTCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACTCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGATTGGCCAGGGCGAAGATCACCCCGCCCt                                                    >  2:380163/1‑150 (MQ=255)
                                                                                                     aTGGTCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGc                                           <  1:943495/150‑1 (MQ=255)
                                                                                                        gTCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGt                                        >  2:1502185/1‑150 (MQ=255)
                                                                                                        gTCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGt                                        >  1:504577/1‑150 (MQ=255)
                                                                                                         tCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTc                                       >  2:552884/1‑150 (MQ=255)
                                                                                                             tctcGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGaa                                                                                    <  2:736278/101‑1 (MQ=255)
                                                                                                             tctcGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGaa                                                                                    >  1:736278/1‑101 (MQ=255)
                                                                                                             tctcGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGc                                   <  1:1630447/150‑1 (MQ=255)
                                                                                                             tctcGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGc                                   <  2:32438/150‑1 (MQ=255)
                                                                                                                cgctcgGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCTTg                                <  2:1434827/150‑1 (MQ=255)
                                                                                                                cgctcgGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCTTg                                <  2:137873/150‑1 (MQ=255)
                                                                                                                 gctcgGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCTTgg                               >  1:541510/1‑150 (MQ=255)
                                                                                                                   tcgGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTc                                       >  1:662905/1‑140 (MQ=255)
                                                                                                                   tcgGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTc                                       <  2:662905/140‑1 (MQ=255)
                                                                                                                    cgGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCTTGGTCa                            <  2:157038/150‑1 (MQ=255)
                                                                                                                      gCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTg                                            <  1:2917/132‑1 (MQ=255)
                                                                                                                      gCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTg                                            >  2:2917/1‑132 (MQ=255)
                                                                                                                      gCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCTTGGTCAgg                          <  1:274844/150‑1 (MQ=255)
                                                                                                                          aCAGAGCCTTGACTAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCTTGGTCAGGGCCa                      <  2:800002/150‑1 (MQ=255)
                                                                                                                             gagCCTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGCTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCTTGGTCAGGGCCAGGc                   >  1:1412841/1‑150 (MQ=255)
                                                                                                                                 cTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTGGGCAGTGCCGTCCAGCTTGGTCAGGGCCAGGCCGGt               <  1:125947/150‑1 (MQ=255)
                                                                                                                                       gAACGGCTCGGCTTCGAAGGTACGCAGGTCGTTGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAAc                                                                                   >  2:916976/1‑76 (MQ=255)
                                                                                                                                       gAACGGCTCGGCTTCGAAGGTACGCAGGTCGTTGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAAc                                                                                   <  1:916976/76‑1 (MQ=255)
                                                                                                                                              tcggctTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGTCAGGGGGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCTTGGTCAGGGCCAGGCCGGTCAGCTCGACGctc  >  1:609051/1‑150 (MQ=255)
                                                                                                                                              tcggctTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCTTGGTCAGGGCCAGGCCGGTCAGCTCGACGctc  >  1:314799/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
CGGTGACGAACAGGAATTCGCCCCGGCGCGCCCGGAAACTCAGCTCATGCAAACCCACATGACCGTTGGGATAGCGCTTGGCAACCTGCTCGAATCGGATCATGGTCAATCTCGCTCGGCGAACAGAGCCTTGACGAACGGCTCGGCTTCGAAGGTACGCAGGTCGTCGATGCCTTCACCGACGCCGATGAAACGGATCGGGATGTTGAACTGCTTGGCCAGGGCGAAGATCACCCCGCCCTTGGCAGTGCCGTCCAGCTTGGTCAGGGCCAGGCCGGTCAGCTCGACGCTC  >  NC_002947/5830610‑5830901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑