Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,882,228 G→C 6.7% E245Q (GAG→CAG)  serA → bifunctional D‑3‑phosphoglycerate dehydrogenase/alpha‑ketoglutarate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,882,2280GC 6.7% 187.2 / 4.2 60E245Q (GAG→CAG) serAbifunctional D‑3‑phosphoglycerate dehydrogenase/alpha‑ketoglutarate reductase
Reads supporting (aligned to +/- strand):  ref base G (29/27);  new base C (2/2);  total (31/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

CTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCTGATCGGCGCCGCCATCGACGTGTTCCCGGTCGAGCCACGCTCCAACGACGAAGAGTTCGAGAGCCCGCTGCGCGGCCTCGACAACGTGATCCTGACGCCGCACATTGGT  >  NC_002947/5882084‑5882377
                                                                                                                                                |                                                                                                                                                     
cTGAACGAGCTGCTGGGCCTGGCCGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTg                                                                                                                                                  <  2:610518/150‑1 (MQ=255)
 tGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTgg                                                                                                                                                 <  1:926310/150‑1 (MQ=255)
          tgctgGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTgg                                                                                                                                        >  2:217986/1‑150 (MQ=255)
              gggCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGc                                                                                                                                    <  1:217986/150‑1 (MQ=255)
                 ccTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGc                                                                                                                                 <  1:1102111/150‑1 (MQ=255)
                       tGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCaa                                                                                                                           >  2:1519935/1‑150 (MQ=255)
                       tGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCaa                                                                                                                           <  2:1030914/150‑1 (MQ=255)
                               tctcGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGc                                                                                                                   >  1:635876/1‑150 (MQ=255)
                                 tcGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCAc                                                                                                                 <  2:635876/150‑1 (MQ=255)
                                 tcGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCAc                                                                                                                 <  1:1535838/150‑1 (MQ=255)
                                       cACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCTGATc                                                                                                           <  2:795940/150‑1 (MQ=255)
                                       cACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCTGATc                                                                                                           <  2:690452/150‑1 (MQ=255)
                                                   cTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCCAGCTCGaccacg                                                                                                                                           >  1:1307131/1‑105 (MQ=255)
                                                   cTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCCAGCTCGaccacg                                                                                                                                           <  2:1307131/106‑2 (MQ=255)
                                                      ccATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTgg                                                                                                                                        <  2:1461949/106‑1 (MQ=255)
                                                      ccATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTgg                                                                                                                                        >  1:1461949/1‑106 (MQ=255)
                                                      ccATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCTGATCGTCGCCGCCATCGAc                                                                                            >  2:576020/1‑150 (MQ=255)
                                                        aTCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCt                                                                                                                                                   >  2:1025614/1‑93 (MQ=255)
                                                        aTCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCt                                                                                                                                                   <  1:1025614/93‑1 (MQ=255)
                                                        aTCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCTGATCGGCGCCGCCATCGACgt                                                                                          >  2:1259020/1‑150 (MQ=255)
                                                            aCCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGaccac                                                                                                                                            >  2:1087389/1‑96 (MQ=255)
                                                            aCCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGaccac                                                                                                                                            <  1:1087389/96‑1 (MQ=255)
                                                            aCCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCTGATCGGCGCCGCCATCGACGTGTTc                                                                                      >  1:687422/1‑150 (MQ=255)
                                                             cccAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCTCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCTGATCGGCGCCGCCATCGACGTGTTcc                                                                                     <  1:841747/150‑1 (MQ=255)
                                                             cccAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCTGATCGTCGCCGCCATCGACGTGTTcc                                                                                     <  1:576020/150‑1 (MQ=255)
                                                             cccAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCTGATCGGCGCCGCCATCGACGTGTTcc                                                                                     <  2:88215/150‑1 (MQ=255)
                                                                  tGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCTGATCGGCGCCGCCATCGACGTGTTCCCGGTc                                                                                >  2:199204/1‑150 (MQ=255)
                                                                    gatgatCGGCGAGAAGGAAATCCGCTCGATGCAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCTGATCGGCGCCGCCATCGACGTGTTCCCgg                                                                                  <  2:793446/146‑1 (MQ=255)
                                                                    gatgatCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCTGATCGGCGCCGCCATCGACGTGTTCCCgg                                                                                  >  1:793446/1‑146 (MQ=255)
                                                                      tgatCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCTGATCGGCGCCGCCATCGACGTGTTCCCGGTCGAGc                                                                            >  1:1369700/1‑150 (MQ=255)
                                                                           ggCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCTGATCGGCGCCGCCATCGACGTGTTCCCGGTCGAGCCACGc                                                                       <  1:894457/150‑1 (MQ=255)
                                                                              gagaAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCTGATCGGCGCCGCCATCGACGTGTTCCCGGTCGAGCCACGCTcc                                                                    >  2:133690/1‑150 (MQ=255)
                                                                                 aaGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCTGATCGGCGCCGCCATCGACgt                                                                                          >  1:1223144/1‑125 (MQ=255)
                                                                                 aaGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCTGATCGGCGCCGCCATCGACgt                                                                                          <  2:1223144/125‑1 (MQ=255)
                                                                                        tCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCTGATCGGCGCCGCCATCGAc                                                                                            >  1:30905/1‑116 (MQ=255)
                                                                                        tCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCTGATCGGCGCCGCCATCGAc                                                                                            <  2:30905/116‑1 (MQ=255)
                                                                                          cGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCTGATCGGCGCCGCCATCGACGTGTTCCCGGTCGAGCCACGCTCCAACGACGAagag                                                        >  2:896076/1‑150 (MQ=255)
                                                                                          cGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCCAGCTCGaccacgg                                                                                                                                          >  2:1131321/1‑66 (MQ=38)
                                                                                          cGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCCAGCTCGaccacgg                                                                                                                                          <  1:1131321/68‑3 (MQ=38)
                                                                                           gCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCTGATCGGCGCCGCCATCGACGTGTTCCCGGTCGAGCCACGCTCCAACGACGAAGAGt                                                       >  1:1116281/1‑150 (MQ=255)
                                                                                               gATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCTGATCGGCGCCGCCATCGACGTGTTCCCGGTCGAGCCACGCTCCAACGACGaa                                                           >  1:839049/1‑142 (MQ=255)
                                                                                               gATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCTGATCGGCGCCGCCATCGACGTGTTCCCGGTCGAGCCACGCTCCAACGACGaa                                                           <  2:839049/142‑1 (MQ=255)
                                                                                                 tGAAGAAGGGCGCGATCCTTATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCTGATCGGCGCCGCCATCGACGTGTTCCCGGTCGAGCCACGCTCCAACGACGAAGAGTTCgaga                                                 >  1:1622221/1‑150 (MQ=255)
                                                                                                 tGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCTGATCGGCGCCGCCATCGACGTGTTCCCGGTCGAGCCACGCTCCAACGACGAAGAGTTCgaga                                                 >  1:879414/1‑150 (MQ=255)
                                                                                                 tGAAGAAGGGCGCGATCCTGATCAACGACGCCAGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCTGATCGGCGCCGCCATCGACGTGTTCCCGGTCGAGCCACGCTCCAACGACGAAGAGTTCgaga                                                 >  2:1252539/1‑150 (MQ=255)
                                                                                                         ggCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCTGATCGGCGCCGCCATCGACGTGTTCCCGGTCGAGCCACGCTCCAACGACGAAGAGTTCGAGAGCCCGCTg                                         >  2:441341/1‑150 (MQ=255)
                                                                                                             cgATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCTGATCGGCGCCGCCATCGACGTGTTCCCGGTCGAGCCACGCTCCAACGACGAAGAGTTCGAGAGCCCGCTGcgcg                                     <  2:687422/150‑1 (MQ=255)
                                                                                                              gATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCTGATCGGCGCCGCCATCGACGTGTTCCCGGTCGAGCCACGCTCCAACGACGAAGAGTTCGAGAGCCCGCTGCGCgg                                    >  1:64631/1‑150 (MQ=255)
                                                                                                                   tGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCTGATCGGCGCCGCCATCGAc                                                                                            <  1:1599209/89‑1 (MQ=255)
                                                                                                                   tGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCTGATCGGCGCCGCCATCGAc                                                                                            >  2:1599209/1‑89 (MQ=255)
                                                                                                                    gATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCaa                                                                                                                           <  2:315984/57‑1 (MQ=255)
                                                                                                                    gATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCaa                                                                                                                           >  1:315984/1‑57 (MQ=255)
                                                                                                                    gATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCTGATCGGCGCCGCCATCGACGTGTTCCCGGTCGAGCCACGCTc                                                                     >  1:1304862/1‑111 (MQ=255)
                                                                                                                    gATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCTGATCGGCGCCGCCATCGACGTGTTCCCGGTCGAGCCACGCTc                                                                     <  2:1304862/111‑1 (MQ=255)
                                                                                                                             cgccCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCTGATCGGCGCCGCCATCGACGTGTTCCCGGTCGAGCCACGCTCCAACGACGAAGAGTTCGAGAGCCCGCTGCGCGGCCTCGACAACGTGAt                     >  2:1108670/1‑150 (MQ=255)
                                                                                                                                     gCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCTGATCGGCGCCGCCATCGACGTGTTCCCGGTCGAGCCACGCTCCAACGACGAAGAGTTCGAGAGCCCGCTGCGCGGCCTCGACAACGTGATCCTGAcgc             >  1:1614394/1‑150 (MQ=255)
                                                                                                                                      cACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCTGATCGGCGCCGCCATCGACGTGTTCCCGGTCGAGCCACGCTCCAACGACGAAGAGTTCGAGAGCCCGCTGCGCGGCCTCGACAACGTGATCCTGAcgcc            <  1:133690/150‑1 (MQ=255)
                                                                                                                                       aCCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCTGATCGGCGCCGCCATCGACGTGTTCCCGGTCGAGCCACGCTCCAACGACGAAGAGTTCGAGAGCCCGCTgcgc                                      >  2:1355649/1‑123 (MQ=255)
                                                                                                                                       aCCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCTGATCGGCGCCGCCATCGACGGGTTCCCGGTCGAGCCACGCTCCAACGACGAAGAGTTCGAGAGCCCGCTgcgc                                      <  1:1355649/123‑1 (MQ=255)
                                                                                                                                               cGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCTGATCGGCGCCGCCATCGACGTGTTCCCGGTCGAGCCACGCTCCAACGACGAAGAGTTCGAGAGCCCGCTGCGCGGCCTCGACAACGTGATCCTGACGCCGCACATtgg   <  1:1108670/150‑1 (MQ=255)
                                                                                                                                                gAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCTGATCGGCGCCGCCATCGACGTGTTCCCGGTCGAGCCACGCTCCAACGACGAAGAGTTCGAGAGCCCGCTGCGCGGCCTCGACAACGTGATCCTGACGCCGCACATtggt  <  2:1614394/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
CTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCTGCCATCAAGGACAAGCACCTGATCGGCGCCGCCATCGACGTGTTCCCGGTCGAGCCACGCTCCAACGACGAAGAGTTCGAGAGCCCGCTGCGCGGCCTCGACAACGTGATCCTGACGCCGCACATTGGT  >  NC_002947/5882084‑5882377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑