Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,883,651 C→G 7.0% A60G (GCC→GGC)  PP_5157 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,883,6510CG 7.0% 181.8 / 5.3 57A60G (GCC→GGC) PP_5157hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (29/24);  new base G (2/2);  total (31/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

GTACTGATCAGTCCATTGGCTCAGGCGGAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGACGGCCCGCTCGATGTAAGCCTGC  >  NC_002947/5883503‑5883800
                                                                                                                                                    |                                                                                                                                                     
gTACTGATCAGTCCATTGGCTCAGGCGGAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGcc                                                                                                                                                      >  2:249178/1‑150 (MQ=255)
   cTGGTCAGTCGAGTGGCTCAGGCGGAGCGGAGCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACAGCGAATTTGGCCAGGCCCTg                                                                                                                                                   <  2:657584/150‑1 (MQ=255)
    tGATCAGTCCATTGGCTCAGGCGGAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTgg                                                                                                                                                  >  2:168860/1‑150 (MQ=255)
           tCCATTGGCTCAGGCGGAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACTCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGa                                                                                                                                           >  2:1051455/1‑150 (MQ=255)
            ccATTGGCTCAGGCGGAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGaa                                                                                                                                          <  1:17987/150‑1 (MQ=255)
             cATTGGCTCAGGCGGAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACTCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAAc                                                                                                                                         <  1:1051455/150‑1 (MQ=255)
                        gCGGAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTgcgc                                                                                                                              <  1:1021929/150‑1 (MQ=255)
                             gCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGa                                                                                                                         <  1:168860/150‑1 (MQ=255)
                                gATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGtt                                                                                                                      >  2:390628/1‑150 (MQ=255)
                                   cgacgaAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTtcgt                                                                                                                   <  2:902469/150‑1 (MQ=255)
                                     acgaAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTtcgtcg                                                                                                                 >  1:472178/1‑150 (MQ=255)
                                             gCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCg                                                                                                         >  1:1098175/1‑150 (MQ=255)
                                              ccGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCATAACAGCAACTCGTCCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCgg                                                                                                        >  2:221968/1‑150 (MQ=255)
                                                     gggCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGAAAGGTTTGGACATCGAATTTGGCCAGGCACTGGACAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGTCCCGGCTagata                                                                                                 >  1:758807/1‑146 (MQ=255)
                                                      ggCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGaa                                                                                                <  2:771016/150‑1 (MQ=255)
                                                             tGCGCATTGCGGTACAGGGCTAGAACGCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTGGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTgg                                                                                         <  2:707043/150‑1 (MQ=255)
                                                             tGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCaa                                                                                                                                              <  2:1255500/97‑1 (MQ=255)
                                                             tGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCaa                                                                                                                                              >  1:1255500/1‑97 (MQ=255)
                                                                gcATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCgg                                                                                      <  1:390628/150‑1 (MQ=255)
                                                                  aTTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGgt                                                                                    >  1:693804/1‑150 (MQ=255)
                                                                       cGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCga                                                                               >  1:954650/1‑150 (MQ=255)
                                                                       cGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCga                                                                               >  1:174325/1‑150 (MQ=255)
                                                                         tACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCgaga                                                                             <  2:693804/150‑1 (MQ=255)
                                                                            aGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTg                                                                          <  1:1538494/150‑1 (MQ=255)
                                                                            aGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTg                                                                          >  1:1578260/1‑150 (MQ=255)
                                                                              gCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGc                                                                        <  2:954650/150‑1 (MQ=255)
                                                                              gCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGc                                                                        >  1:857141/1‑150 (MQ=255)
                                                                                            aTACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGc                                                          >  2:1103643/1‑150 (MQ=255)
                                                                                               cGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCAtt                                                       <  2:857141/150‑1 (MQ=255)
                                                                                                  cTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGaa                                                                                                               >  1:1484658/1‑91 (MQ=255)
                                                                                                  cTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGaa                                                                                                               <  2:1484658/91‑1 (MQ=255)
                                                                                                  cTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCAc                                                    <  2:472178/150‑1 (MQ=255)
                                                                                                      aagcaagACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCg                                                >  2:284597/1‑150 (MQ=255)
                                                                                                        gcaagACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCga                                                                               >  1:451033/1‑117 (MQ=255)
                                                                                                        gcaagACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCga                                                                               <  2:451033/117‑1 (MQ=255)
                                                                                                        gcaagACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGagag                                                                            >  1:175818/1‑120 (MQ=255)
                                                                                                        gcaagACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGagag                                                                            <  2:175818/120‑1 (MQ=255)
                                                                                                          aagACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTTGCGGGTGTTGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGa                                            >  1:513485/1‑150 (MQ=255)
                                                                                                                     cTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGaa                                       <  2:947591/144‑1 (MQ=255)
                                                                                                                     cTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGaa                                       >  1:947591/1‑144 (MQ=255)
                                                                                                                        aCAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTcaca                              >  1:1358464/1‑150 (MQ=255)
                                                                                                                             tttCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGaa                                                                                                <  2:1427962/79‑1 (MQ=255)
                                                                                                                             tttCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGaa                                                                                                >  1:1427962/1‑79 (MQ=255)
                                                                                                                             tttCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGa                         >  2:1566502/1‑150 (MQ=255)
                                                                                                                                  aCATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTgg                                                                         >  2:1086073/1‑97 (MQ=255)
                                                                                                                                  aCATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTgg                                                                         <  1:1086073/97‑1 (MQ=255)
                                                                                                                                    aTCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGACGGCCCg                  >  2:216379/1‑150 (MQ=255)
                                                                                                                                     tCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTcaca                              >  1:346963/1‑137 (MQ=255)
                                                                                                                                     tCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTcaca                              <  2:346963/137‑1 (MQ=255)
                                                                                                                                       gAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGACGGCCCGCTc               >  1:656759/1‑150 (MQ=255)
                                                                                                                                         ccttGGCCAGGGCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCAtt                                                       <  1:242982/106‑1 (MQ=255)
                                                                                                                                         ccttGGCCAGGGCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCAtt                                                       >  2:242982/3‑108 (MQ=255)
                                                                                                                                           ttGGCCAGGGCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGgtg                                                                                   >  1:18165/1‑78 (MQ=255)
                                                                                                                                           ttGGCCAGGGCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGgtg                                                                                   <  2:18165/78‑1 (MQ=255)
                                                                                                                                            tGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGACGGCCCGCTCGATGt          <  2:1525715/150‑1 (MQ=255)
                                                                                                                                                cAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTc                                                                                 <  2:302839/75‑1 (MQ=255)
                                                                                                                                                cAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTc                                                                                 >  1:302839/1‑75 (MQ=255)
                                                                                                                                                cAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGACGGCCCGCTCGATGTAAGc      >  1:1215658/1‑150 (MQ=255)
                                                                                                                                                    cccTGGCCAAGGAACTGGATTTGCGCGCCGGGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTCAGGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGACGGCCCGCTCGATGTAAGCCTGc  <  1:1103643/150‑1 (MQ=255)
                                                                                                                                                    cccTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCCCCCCGCCGATTGAATCGCTCACAGGCGACGGCCCGCTCGATGTAAGCCTGc  <  1:284597/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GTACTGATCAGTCCATTGGCTCAGGCGGAGCTGATCGACGAAATCGCCGACCGGGGCGAACTGCGCATTGCCGTACAGGCCGAGAACTCGCCATACGCCTTCAAGCAAGACGACCACCTGACAGGTTTCGACATCGAATTTGGCCAGGCCCTGGCCAAGGAACTGGATTTGCGCGCCGAGTTCGTCGAAGCCCCGGCTACCGAAGTGCTGGCGGGTGTCGAGAGTGGCAAGTACGACATCGCATTCACCCCGTCGAGTGAATCGCTCACAGGCGACGGCCCGCTCGATGTAAGCCTGC  >  NC_002947/5883503‑5883800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑