Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,890,849 G→T 7.9% intergenic (+81/+39) PP_5164 → / ← plpB penicillin amidase family protein/NLPA lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,890,8490GT 7.9% 195.6 / 3.9 62intergenic (+81/+39)PP_5164/plpBpenicillin amidase family protein/NLPA lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (25/32);  new base T (3/2);  total (28/34)
Fisher's exact test for biased strand distribution p-value = 6.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

CAGTATCAGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATGGCCTCACTGTCCTTGTTG  >  NC_002947/5890700‑5890996
                                                                                                                                                     |                                                                                                                                                   
cAGTATCAGCGGGCGCAGATGGGGGTGATTCCGGCGCCAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGCGCTGAAGAAAAACGCCGACACAAg                                                                                                                                                     >  2:1358697/1‑150 (MQ=255)
  gTATCAGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGgt                                                                                                                                                   <  1:1217163/150‑1 (MQ=255)
  gTATCAGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGgt                                                                                                                                                   <  1:313596/150‑1 (MQ=255)
       aGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAg                                                                                                                                                     <  2:958988/143‑1 (MQ=255)
       aGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAg                                                                                                                                                     >  1:958988/1‑143 (MQ=255)
         cGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCg                                                                                                                                            <  2:1241059/150‑1 (MQ=255)
            gcgcAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCTCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGCGCGGAAGAAAAACGCCGACACAAGGTGTCGGCGTTg                                                                                                                                         <  1:544449/150‑1 (MQ=255)
             cgcgtaggggtgTAATTGCGACGCTAAGTCCCGTACGGCTTGTACCCCCAAAGTAAGAGGCTGCTCGGCGACCGGTTCGCAGAAAAAGGCTTCTACGGTGCTGTGCAGGCAGGCCTGAAGAAAACCGCCGACACAAGTTGTCGGCGTTgg                                                                                                                                        <  2:988764/143‑1 (MQ=255)
                            ttCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGtt                                                                                                                         >  1:948796/1‑150 (MQ=255)
                              ccGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGc                                                                                                                       <  2:283382/150‑1 (MQ=255)
                                      aaGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGaaa                                                                                                               >  2:1046678/1‑150 (MQ=255)
                                        gTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATc                                                                                                             <  2:1274563/150‑1 (MQ=255)
                                        gTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATc                                                                                                             <  1:1046678/150‑1 (MQ=255)
                                         tACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCa                                                                                                            >  2:1019000/1‑150 (MQ=255)
                                             ttACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGaa                                                                                                         >  2:105152/1‑149 (MQ=255)
                                             ttACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGaa                                                                                                         <  1:105152/149‑1 (MQ=255)
                                                  gCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTCGCTCAATCAGAACGCCgg                                                                                                   <  1:1290915/150‑1 (MQ=255)
                                                          ccccGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACgg                                                                                           <  1:542588/150‑1 (MQ=255)
                                                           cccGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAGAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGc                                                                                          >  2:1524164/1‑150 (MQ=255)
                                                           cccGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGc                                                                                          >  1:1437676/1‑150 (MQ=255)
                                                            ccGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGTAGGCCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCa                                                                                         >  2:1635780/1‑150 (MQ=255)
                                                            ccGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCa                                                                                         >  2:899034/1‑150 (MQ=255)
                                                                     gagGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTAc                                                                                >  1:646577/1‑150 (MQ=255)
                                                                       ggCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGGAGGGCTGAAGAAAAACGCCATCACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTActt                                                                              >  1:1434714/1‑150 (MQ=255)
                                                                        gctgctTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTActtc                                                                             >  2:1354023/1‑150 (MQ=255)
                                                                             tttGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGc                                                                        >  1:1375174/1‑150 (MQ=255)
                                                                                gcgcCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCaat                                                                     <  1:683399/150‑1 (MQ=255)
                                                                                  ggCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCGCGGCGCTTGCAGTACGGCACCTTGGTACTTCTTGGCaatga                                                                   >  1:904491/1‑150 (MQ=255)
                                                                                  ggCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCaatga                                                                   <  2:878763/150‑1 (MQ=255)
                                                                                         tCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCtt                                                            <  2:646577/150‑1 (MQ=255)
                                                                                           gcagcaAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTa                                                                                                                     >  2:1353141/1‑91 (MQ=255)
                                                                                           gcagcaAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTa                                                                                                                     <  1:1353141/91‑1 (MQ=255)
                                                                                             agcaAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCa                                                                                                                               >  1:961791/1‑79 (MQ=255)
                                                                                             agcaAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCa                                                                                                                               <  2:961791/79‑1 (MQ=255)
                                                                                              gcaAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCa                                                                                                 <  2:1093108/108‑1 (MQ=255)
                                                                                              gcaAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCa                                                                                                 >  1:1093108/1‑108 (MQ=255)
                                                                                                 aaaGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGaa                                                                                                         >  2:1119772/1‑97 (MQ=255)
                                                                                                 aaaGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGaa                                                                                                         <  1:1119772/97‑1 (MQ=255)
                                                                                                       ttCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGc                                                                        <  2:307377/124‑1 (MQ=255)
                                                                                                       ttCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGc                                                                        >  1:307377/1‑124 (MQ=255)
                                                                                                            cGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAgg                                         <  1:306085/150‑1 (MQ=255)
                                                                                                            cGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAgg                                         <  2:1437676/150‑1 (MQ=255)
                                                                                                                 cTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAATGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCtt                                    <  1:363212/150‑1 (MQ=255)
                                                                                                                      cGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCa                               <  1:1202976/150‑1 (MQ=255)
                                                                                                                              gCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTgg                       >  1:33888/1‑150 (MQ=255)
                                                                                                                               cTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGGACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGa                      <  1:1358697/150‑1 (MQ=255)
                                                                                                                                tGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGAt                     >  2:1246470/1‑150 (MQ=255)
                                                                                                                                tGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGAt                     <  1:1524164/150‑1 (MQ=255)
                                                                                                                                tGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGAt                     >  1:208577/1‑150 (MQ=255)
                                                                                                                                  aagaaAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATgg                   <  2:948796/150‑1 (MQ=255)
                                                                                                                                    tgccaaCGCCGACACCTTGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATGGcc                 >  1:1384941/5‑150 (MQ=255)
                                                                                                                                    gaaAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATGAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATGGcc                 <  1:376052/150‑1 (MQ=255)
                                                                                                                                    gaaAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCggg                                                  <  2:1059987/117‑1 (MQ=255)
                                                                                                                                    gaaAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCggg                                                  >  1:1059987/1‑117 (MQ=255)
                                                                                                                                       caaCGCCGACACCTTGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATGGCCTCa              >  2:499671/2‑150 (MQ=255)
                                                                                                                                        aaCGCCGACACCTTGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGAc                                                         >  2:239227/1‑106 (MQ=255)
                                                                                                                                        aaCGCCGACACCTTGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGAc                                                         <  1:239227/106‑1 (MQ=255)
                                                                                                                                         aCGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTgg                                                                                   <  1:858789/79‑1 (MQ=255)
                                                                                                                                         aCGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTgg                                                                                   >  2:858789/1‑79 (MQ=255)
                                                                                                                                               acacAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATGGCCTCACTGTCCtt      <  2:208577/150‑1 (MQ=255)
                                                                                                                                                cacaAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATGGCCTCACTGTCCttg     <  2:33888/150‑1 (MQ=255)
                                                                                                                                                  caAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATGGCCTCACTGTCCttgtt   <  1:1635780/150‑1 (MQ=255)
                                                                                                                                                   aaGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTg                                                                          >  1:1266419/1‑78 (MQ=255)
                                                                                                                                                   aaGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTg                                                                          <  2:1266419/78‑1 (MQ=255)
                                                                                                                                                   aaGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATGGCCTCACTGTCCttgttg  >  1:338853/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CAGTATCAGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATGGCCTCACTGTCCTTGTTG  >  NC_002947/5890700‑5890996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑