| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,914,184 | A→G | 13.1% | intergenic (+20/+133) | spuI → / ← argA | glutamylpolyamine synthetase/amino acid N‑acetyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,914,184 | 0 | A | G | 13.1% | 153.3 / 13.3 | 61 | intergenic (+20/+133) | spuI/argA | glutamylpolyamine synthetase/amino acid N‑acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (24/29); new base G (3/5); total (27/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.10e-01 | |||||||||||
ATCCTGAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCTGTGGGAGCGGGCGTGCCCGCGAAGAGGGCGGCCCAGGCTTACAGGG > NC_002947/5914039‑5914324 | aTCCTGAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGggc < 1:187330/150‑1 (MQ=255) tCCTGAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAggg > 2:1375194/1‑148 (MQ=255) tCCTGAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAggg < 1:1375194/148‑1 (MQ=255) cTGAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGggcggc > 2:441802/1‑150 (MQ=255) tGAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGgcggcg < 2:217727/150‑1 (MQ=255) tGAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACAAAAACGCCGCCCTTGAGGGgcggcg > 2:605354/1‑150 (MQ=255) aaCAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTTGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTTAAGGGCGGCGtt < 1:1327474/150‑1 (MQ=255) aaCAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACAAAAACGCCGCCCTTGAGGGGCGGCGtt < 1:605354/150‑1 (MQ=39) aaGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACAAAAACGCCGCCCTTGAGGGGCGGCGttttc < 2:134473/150‑2 (MQ=38) aGTACTTAGATCCTAAATACATTGCCATTTTCCTCGCGTGCAAGTAAAGCGAGCTGGAATTGTTCTAGCCCTCGATCTCCGACCTCCAGTACAACTGGTACCTGCATACCGTGTAAACAAATACGCCGCCCTTGAGGGGCGGCGttttcg < 1:540850/150‑3 (MQ=255) aTCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTga < 1:798831/150‑1 (MQ=255) tCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCg < 1:1609344/150‑1 (MQ=255) ccGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCgg < 1:660806/150‑1 (MQ=255) aTCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCct > 1:1522269/1‑150 (MQ=255) gtcgCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCAc < 1:441802/150‑1 (MQ=255) gcgTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGGCCCTCAAggg < 1:164327/104‑1 (MQ=255) gcgTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAggg > 2:164327/1‑104 (MQ=255) tGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGc > 2:802130/1‑150 (MQ=255) aaGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTcc > 1:8949/1‑150 (MQ=255) aGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAgg < 2:701777/150‑1 (MQ=255) aGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAgg < 2:1522269/150‑1 (MQ=255) cGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGt < 1:802130/150‑1 (MQ=255) gTTCGAGCACTCGATCTCCGACCTCGATTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTga < 2:666422/94‑1 (MQ=255) gTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTga > 1:666422/1‑94 (MQ=255) gAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCCGCCGCCGGGCACTTTCGCAggatcga > 2:300201/1‑145 (MQ=255) tCGATCTCCCACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCtgt < 1:213773/150‑1 (MQ=255) gATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTcgc < 1:573043/103‑1 (MQ=255) gATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTcgc > 2:573043/1‑103 (MQ=255) gATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGa < 1:300201/150‑1 (MQ=255) gATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGa > 2:1301884/1‑150 (MQ=255) ctcCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACtt > 1:13007/1‑150 (MQ=255) tcCGACGGCGAGTACGACTGGTACCTGGATAGCGGGTAAAGGAAAACGCCGCCCCTCAAGGGCGGCGTTGTTGTTTGAGACCGGTGTGGCCCTCTTGGCGGGCAGGCCCGCTGCCGCAGGGGAATTTGGAGGGCTGAAGCGTGTGACTTa > 2:1256453/1‑150 (MQ=255) tcCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTa < 1:1301884/150‑1 (MQ=255) ccGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTAc < 2:13007/150‑1 (MQ=255) ccTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCtgt < 1:1119899/139‑1 (MQ=255) ccTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCACGCAGGGTAATTTCGAAGGCTGAAGCCtgt > 2:1119899/1‑139 (MQ=255) tACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCtgt > 2:461498/1‑132 (MQ=255) tACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCtgt < 1:461498/132‑1 (MQ=255) tGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCTGTGGGAGCGGGCGTg > 1:1077977/1‑150 (MQ=255) cTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCTGTGGGAGCGGGCGTGCCcgcg < 2:1077977/150‑1 (MQ=255) tACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCTGTGGGAGCggg > 1:262793/1‑135 (MQ=255) tACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCTGTGGGAGCggg < 2:262793/135‑1 (MQ=255) tctcaaaCAAAAACGCCGCCCTTGAGGGGCGGCGTTTTCGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCtgtg < 1:640915/119‑1 (MQ=37) tctcaaaCAAAAACGCCGCCCTTGAGGGGCGGCGTTTTCGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCtgtg > 2:640915/5‑123 (MQ=37) tgtAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCtgt > 1:1503967/1‑110 (MQ=255) tgtAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCtgt < 2:1503967/110‑1 (MQ=255) tgtAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCtgt > 1:367081/1‑121 (MQ=255) tgtAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCtgt < 2:367081/121‑1 (MQ=255) aaCGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCTGTGGGAGCGGGCGt > 2:1550189/1‑129 (MQ=255) aaCGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCTGTGGGAGCGGGCGt < 1:1550189/129‑1 (MQ=255) acaaaaaCGCCGCCCTTGAGGGGCGGCGTTTTCGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGcc > 2:1584957/4‑69 (MQ=17) acaaaaaCGCCGCCCTTGAGGGGCGGCGTTTTCGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGcc < 1:1584957/66‑1 (MQ=17) cGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCTGTGGGAGCGGGCGTGCCCGCGAAGAgg < 2:1557815/140‑1 (MQ=255) cGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCGCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCTGTGGGAGCGGGCGTGCCCGCGAAGAgg > 1:1557815/1‑140 (MQ=255) aaCGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCTGTGGGAGCGGGCGTGCCCGCGAAGAgg > 1:408693/1‑136 (MQ=255) aaCGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCTGTGGGAGCGGGCGTGCCCGCGAAGAgg < 2:408693/136‑1 (MQ=255) aCGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGaa < 1:87274/86‑1 (MQ=255) aCGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGaa > 2:87274/1‑86 (MQ=255) cgccCCTCAAGGGCGGCGTTTTTGTGAGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCTGTGGGAGCGGGCGTGCCCGCGAAGAGGGCGGCCCAGGCTTACAggg > 1:631556/1‑150 (MQ=255) ccTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCTGTGGGAGCGGGCGTGCCcgc > 2:630396/1‑120 (MQ=255) ccTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCTGTGGGAGCGGGCGTGCCcgc < 1:630396/120‑1 (MQ=255) | ATCCTGAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGGGCGGCGTTTTTGTTTGAGACCGGTGTCGCCCTCTTCGCGGGCACGCCCGCTCCCGCAGGGTAATTTCGAAGGCTGAAGCCTGTGACTTACCTGTGGGAGCGGGCGTGCCCGCGAAGAGGGCGGCCCAGGCTTACAGGG > NC_002947/5914039‑5914324 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |