| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,921,504 | A→G | 6.7% | E91G (GAG→GGG) | PP_5191 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,921,504 | 0 | A | G | 6.7% | 175.4 / 2.4 | 60 | E91G (GAG→GGG) | PP_5191 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (28/28); new base G (2/2); total (30/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.10e-01 | |||||||||||
TCGCGGGATCACAAGCTGGTAGTGGCCGCGCAGGATGACGCCAGCAGCTTCGTCGCCAGCGAAGGCGCGATCCGCGGGCCGTTCCTGGAAGCGGCCATGCGCGAGGCACGGGCAGAGAACCCAGGGATGCAGGCTTCCGACATGGAGTTGGCCAATGCCATCCTGGCCAAGAATGCGGTAGCCGAGTAACTGCGTCGCCTGCTTCGCGGGTGAACCCGCTCCTACAGGCACCCGCGAAGCATCCAACACCAACCTCAGCGATAAGCCTCTACCGGCACACACGCACAGAACA > NC_002947/5921359‑5921650 | tCGCGGGATCACAAGCTGGTAGTGGCCGCGCAGGATGACGCCAGCAGCTTCGTCGCCCGCGAAGGCGCGATCCGCGGGCCGTTCCTGGAAGCGGCCATGCGCGAGGCACGGGCAGAGAACCCAGGGATGCAGGCTTCCGACATGGAGTTg > 2:767482/1‑150 (MQ=255)tCGCGGGATCACAAGCTGGTAGTGGCCGCGCAGGATGACGCCAGCAGCTTCGTCGCCAGCGAAGGCGCGATCCGCGGGCCGTTCCTGGAAGCGGCCATGCGCGATACACGTTCATAGAACCCAGGGATGCAGGCTTCCGACATGGAGTTg > 2:1504223/1‑150 (MQ=255)tCGCGGGATCACAAGCTGGTAGTGGCCGCGCAGGATGACGCCAGCAGCTTCGTCGCCAGCGAAGGCGCGATCCGCGGGCCGTTCCTGGAAGCGGCCATGCGCGAGGCACGGGCAGAGAACCCAGGGATGCAGGCTTCCGACATGGAGTTg < 1:1314376/150‑1 (MQ=255) gcgGGATCACAAGCTGGTAGTGGCCGCGCAGGATGACGCCAGCAGCTTCGTCGCCAGCGAAGGCGCGATCCGCGGGCCGTTCCTGGAAGCGGCCATGCGCGAGGCACGGGCAGAGAACCCAGGGATGCAGGCTTCCGACATGGa < 2:369238/144‑1 (MQ=255) gcgGGATCACAAGCTGGTAGTGGCCGCGCAGGATGACGCCAGCAGCTTCGTCGCCAGCGAAGGCGCGATCCGCGGGCCGTTCCTGGAAGCGGCCATGCGCGAGGCACGGGCAGAGAACCCAGGGATGCAGGCTTCCGACATGGa > 1:369238/1‑144 (MQ=255) tCACAAGCTGGTAGTGGCCGCGCAGGATGACGCCAGCAGCTTCGTCGCCAGCGAAGGCGCGATCCGCGGGCCGTTCCTGGAAGCGGCCATGCGCGAGGCACGGGCAGAGAACCCAGGGATGCCGTCTTCCGACATGGAGTTGGCCAATGc > 2:1199362/1‑150 (MQ=255) tCACAAGCTGGTAGTGGCCGCGCAGGATGACGCCAGCAGCTTCGTCGCCAGCGAAGGCGCGATCCGCGGGCCGTTCCTGGAAGCGGCCATGCGCGAGGCACGGGCAGAGAACCCAGGGATGCAGGCTTCCGACATGGAGTTGGCCAATGc > 1:1544139/1‑150 (MQ=255) acaAGCTGGTAGTGGCCGCGCAGGATGACGCCAGCAGCTTCGTCGCCAGCGAAGGCGCGATCCGCGGGCCGTTCCTGGAAGCGGCCATGCGCGAGGCACGGGCAGAGAACCCAGGGATGCAGGCTTCCGACATGGAGTTGGCCAATGCCa < 1:398011/150‑1 (MQ=255) caAGCTGGTAGTGGCCGCGCAGGATGACGCCAGCAGCTTCGTCGCCAGCGAAGGCGCGATCCGCGGGCCGTTCCTGGAAGCGGCCATGCGCGAGGCACGGGCAGAGAACCCAGGGATGCAGGCTTCCGACATGGAGTTGGCCAATGCCAt > 1:728510/1‑150 (MQ=255) aaGCTGGTAGTGGCCGCGCAGGATGACGCCAGCAGCTTCGTCGCCCGCGAAGGCGCGATCCGCGGGCCGTTCCTGGAAGCGGCCATGCGCGAGGCACGGGCAGAGAACCCAGGGATGCAGGCTTCCGACATGGAGTTGGCCAATGCCa < 1:1600665/148‑1 (MQ=255) aaGCTGGTAGTGGCCGCGCAGGATGACGCCAGCAGCTTCGTCGCCAGCGAAGGCGCGATCCGCGGGCCGTTCCTGGAAGCGGCCATGCGCGAGGCACGGGCAGAGAACCCAGGGATGCAGGCTTCCGACATGGAGTTGGCCAATGCCa > 2:1600665/1‑148 (MQ=255) aGCTGGTAGTGGCCGCGCAGGATGACGCCAGCAGCTTCGTCGCCAGCGAAGCTGCTCTGCTCGGGCCGTTCCTGGAAGCGGCCATGCGCGAGGCACGGGCAGAGAACCCAGGGATGCAGGCTTCCGACATGGAGTTGGCCAATGCCATcc < 2:1041574/150‑1 (MQ=255) cTGGTAGTGGCCGCGCAGGATGACGCCAGCAGCTTCGTCGACAGCGAAGGCGCGATCCGCGGGCCGTTCCTGGAAGCGGCCATGCGCGAGGCACGGGCAGAGAACCCAGGGATGCAGGCTTCCGACATGGAGTTGGCCAATGCCATCCTg < 1:1504223/150‑1 (MQ=255) gTAGTGGCCGCGCAGGATGACGCCAGCAGCTTCGTCGCCAGCGAAGGCGCGATCCGCGGGCCGTTCCTGGAAGCGGCCATGCGCGAGGCACGGGCAGAGAACCCAGGGATGCAGGCTTCCGACATGGAGTTGGCCAATGCCATCCTGGcc > 1:1180897/1‑150 (MQ=255) aGTGGCCGCGCAGGATGACGCCAGCAGTTTCGTCGCCAGCGAAGGCGCGATCCTCGGGCCGTTCCTGGAAGCGGCCATGCGCGAGGCACGGGCAGAGAACCCAGGGATGCAGGCTTCCGACATGGAGTTGGCCAATGCCATCCTGGCCaa < 2:863512/150‑1 (MQ=255) aGTGGCCGCGCAGGATGACGCCAGCAGCTTCGTCGCCAGCGAAGGCGCGATCCGCGGGCCGTTCCTGGAAGCGGCCATGCGCGAGGCACGGGCAGAGAACCCAGGGATGCAGGCTTCCGACATGGAGTTGGCCAATGCCATCCTGGCCaa > 2:1259610/1‑150 (MQ=255) ggCCGCGCAGGATGACGCCAGCAGCTTCGTCGCCAGCGAAGGCGCGATCCGCGGGCCGTTCCTGGAAGCGGCCATGCGCGAGGCACGGGCAGAGAACCCAGGGATGCAGGCTTCCGACATGGAGTTGGCCAATGCCATCCTGGCCAAGaa < 1:238284/150‑1 (MQ=255) ggCCGCGCAGGATGACGCCAGCAGCTTCGTCGCCAGCGAAGGCGCGATCCGCGGGCCGTTCCTGGAAGCGGCCATGCGCGAGGCACGGGCAGAGAACCCAGGGATGCAGGCTTCCGACATGGAGTTGGCCAATGCCATCCTGGCCAAGaa < 2:1180897/150‑1 (MQ=255) cctcgcgCAGGATGACGCCAGCAGCTTCGTCGCCAGCGAAGGCGCGATCCGCGGGCCGTTCCTGGAAGCGGCCATGCGCGAGGCACGGGCAGAGAACCCAGGGATGCAGGCTTCCGACATGGAGTTGGCCAATGCCATCCTGGCCAAGaa > 2:73637/4‑150 (MQ=255) cgcgCAGGATGACGCCAGCAGCTTCGTCGCCAGCGAAGGCGCGATCCGCGGGCCGTTCCTGGAAGCGGCCATGCGCGAGGCACGGGCAGAGAACCCAGGGATGCAGGCTTCCGACATGGAGTTGGCCAATGCCATCCTGGCCAAGAATGc < 1:1259610/150‑1 (MQ=255) gATGACGCCAGCAGCTTCGTCGCCAGCGAAGGCGCGATCCGCGGGCCGTTCCTGGAAGCGGCCATGCGCGAGGCACGGGCAGAGAACCCAGGGATGCAGGCTTCCGACATGGAGGTGGCCAATGCCATCCTGGCCAAGAATGCGGTAGcc > 1:1437315/1‑150 (MQ=255) gCCAGCAGCTTCGTCGCCAGCGAAGGCGCGATCCGCGGGCCGTTCCTGGAAGCGGCCATGCGCGAGGCACGGGCAGAGACCACAGGGATGCAGGCTTCCGACAGGGAGTTGGCCAATGCCATCCTGGCCAAGAATGCGGTAGCCGAGTaa > 1:1263369/1‑150 (MQ=255) gCCAGCAGCTTCGTCGCCAGCGAAGGCGCGATCCGCGGGCCGTTCCTGGAAGCGGCCATGCGCGAGGCACGGGCAGAGAACCCAGGGATGCAGGCTTCCGACATGGAGTTGGCCAATGCCATCCTGGCCAAGAATGCGGTAGCCGAGTaa > 2:1376235/1‑150 (MQ=255) ccAGCAGCTTCGTCGCCAGCGAAGGCGCGATCCGCGGGCCGTTCCTGGAAGCGGCCATGCGCGAGGCACGGGCAGAGAACCCAGGGATGCAGGCTTCCGACATGGAGTTGGCCAATGCCATCCTGGCCAAGAATGCGGTAGCCGAGTAAc > 2:1217501/1‑150 (MQ=255) cagcagCTTCGTCGCCAGCGAAGGCGCGATCCGCGGGCCGTTCCTGGAAGCGGCCATGCGCGAGGCACGGGCAGAGAACCCAGGGATGCAGGCTTCCGACATGGGGTTGGCCAATGCCATCCTGGCCAAGAATGCGGTAGCCGAGTAACt > 1:710900/1‑150 (MQ=255) ttCGTCGCCAGCGAAGGCGCGATCCGCGGGCCGTTCCTGGAAGCGGCCATGCGCGAGGCACGGGCAGAGAACCCAGGGATGCAGGCTTCCGACATGGGGTTGGCCAATGCCATCCTGGCCAAGAATGCGGTAGCCGa > 2:680791/1‑137 (MQ=255) ttCGTCGCCAGCGAAGGCGCGATCCGCGGGCCGTTCCTGGAAGCGGCCATGCGCGAGGCACGGGCAGAGAACCCAGGGATGCAGGCTTCCGACATGGGGTTGGCCAATGCCATCCTGGCCAAGAATGCGGTAGCCGa < 1:680791/137‑1 (MQ=255) cgtcgCCAGCGAAGGCGCGATCCGCGGGCCGTTCCTGGAAGCGGCCATGCGCGAGGCACGGGCAGAGAACCCAGGGATGCAGGCTTCCGACATGGAGTTCGCCAAGTCAATCCTGGCCAAGAATGCGGTAGCCGAGTAACTGCGTCGCCt < 1:1199362/150‑1 (MQ=255) cAGCGAAGGCGCGATCCGCGGGCCGTTCCTGGAAGCGGCCATGCGCGAGGCACGGGCAGAGAACCCAGGGATGCAGGCTTCCGACATGGAGTTGGCCAATGCCATCCTGGCCAAGAATGCGGTAGCCGAGTAACTGCGTCGCCTGCTTcg > 2:1076332/1‑150 (MQ=255) gCGAAGGCGCGATCCGCGGGCCGTTCCTGGAAGCGGCCATGCGCGAGGCACGGGCAGAGAACCCAGGGATGCAGGCTTCCGACATGGAGTTGGCCAATGCCATCCTGGCCAAGAATGCGGTa < 2:311584/122‑1 (MQ=255) gCGAAGGCGCGATCCGCGGGCCGTTCCTGGAAGCGGCCATGCGCGAGGCACGGGCAGAGAACCCAGGGATGCAGGCTTCCGACATGGAGTTGGCCAATGCCATCCTGGCCAAGAATGCGGTa > 1:311584/1‑122 (MQ=255) gAAGGCGCGATCCGCGGGCCGTTCCTGGAAGCGGCCATGCGCGAGGCACGGGCAGAGAACCCAGGGATGCAGGCTTCCGACATGGAGTTGGCCAATGCCATCCTGGCCAAGAATGCGGTAGCCGAGTAACTGCGTCGCCTGCTTCGCggg < 1:1217501/150‑1 (MQ=255) cgcgATCCGCGGGCCGTTCCTGGAAGCGGCCATGCGCGAGGCACGGGCAGAGAACCCAGGGATGCAGGCTTCCGACATGGAGTTGGCCAATGCCATCCTGGCCAAGAATGCGGTAGCCGAGTAACTGCGTCGCCTGCTTCGCGGGTGAAc < 1:675547/150‑1 (MQ=255) gccGCGGGCCGTGCCTGGAAGCGGCCATGCGCGAGGCACGGGCAGAGCAACCAGGGATGCAGGCTTCCGACATGGAGTTGGCCAATGCCATCCTGGCCAAGAATGCGGTAGCCGAGTAACTGCGTCGCCTGCTTCGCGGGTGAACCCGCt < 1:100392/149‑1 (MQ=255) cgGGCCGTTCCTGGAAGCGGCCATGCGCGAGGCACGGGCAGAGAACCCAGGGATGCAGGCTTCCGACATGGAGTTGGCCAATGCCATCCTGGCCAAGAATGCGGTAGCCGAGTAACTGCGTCGCCTGCTTCGCGGGTGAACCCGCTCCTa < 1:1645375/150‑1 (MQ=255) gggCCGTTCCTGGAAGCGGCCATGCGCGAGGCACGGGCAGAGAACCCAGGGATGCAGGCTTCCGACATGGAGTTGGCCAATGCCATCCTGGCCAAGAATGCGGTAGCCGAGTAACTGCGTCGCCTGCTTCGCGGGTGAACCCGCTCCTAc > 2:389895/1‑150 (MQ=255) ggCCGTTCCTGGAAGCGGCCATGCGCGAGGCACGGGCAGAGAACCCAGGGATGCAGGCTTCCGACATGGAGTTGGCCAATGCCATCCTGGCCAAGAATGCGGTAGCCGAGTAACTGCGTCGCCTGCTTCGCGGGTGAACCCGCTCCTACa < 2:1079169/150‑1 (MQ=255) cTGGAAGCGGCCATGCGCGAGGCACGGGCAGAGAACCCAGGGATGCAGGCTTCCGACATGGAGTTGGCCAATGCCATCCTGGCCAAGAATGCGGTAGCCGAGTAACTGCGTCGCCTGCTTCGCGGGTGAACCCGCTCCTACAGGCACCcg > 1:644536/1‑150 (MQ=255) aaGCGGCCATGCGCGAGGCACGGGCAGAGAACCCAGGGATGCAGGCTTCCGACATGGAGTTGGCCAATGCCATCCTGGCCAAGAATGCGGTAGCCGAGTAACTGCGTCGCCTGCTTCGCGGGTGAACCCGCTCCTACAGGCACCCGCGaa > 1:19489/1‑150 (MQ=255) cATGCGCGAGGCACGGGCAGAGAACCCAGGGATGCAGGCTTCCGACATGGAGTTGGCCAATGCCATCCTGGCCAAGAATGCGGTAGCCGAGTAACTGCGTCGCCTGCTTCGCGGGTGAACCCGCTCCTACAGGCACCCGCGAAGCATCCa > 2:1084908/1‑150 (MQ=255) gcgAGGCACGGGCAGAGAACCCAGGGATGCAGGCTTCCGACATGGAGTTGGCCaa > 1:1507053/1‑55 (MQ=255) gcgAGGCACGGGCAGAGAACCCAGGGATGCAGGCTTCCGACATGGAGTTGGCCaa < 2:1507053/55‑1 (MQ=255) gAGGCACGGGCAGAGAACCCAGGGATGCAGGCTTCCGACATGGAGTTGGCCAATGCCATCCTGGCCAAGAATGCGGTAGCCGAGTAACTGCGTc < 2:1495402/94‑1 (MQ=255) gAGGCACGGGCAGAGAACCCAGGGATGCAGGCTTCCGACATGGAGTTGGCCAATGCCATCCTGGCCAAGAATGCGGTAGCCGAGTAACTGCGTc > 1:1495402/1‑94 (MQ=255) aGGCACGGGCAGAGAACCCAGGGATGCAGGCTTCCGACATGGAGTTGGCCAATGCCATCCTGGCCAAGAATGCGGTAGCCGAGTAACTGCGTCGCCTGCTTCGCGGGTGAACCCGCTCCTACAGGCACCCGCGAAGCATCCAACACCAAc < 1:1376235/150‑1 (MQ=255) aGGCACGGGCAGAGAACCCAGGGATGCAGGCTTCCGACATGGAGTTGGCCAATGCCATCCTGGCCAAGAATGCGGTAGCCGAGTAACTGCGTCGCCTGCTTCGCGGGTGAACCCGCTCCTACAGGCACCCGCGAAGCATCCAACACCAAc < 2:19489/150‑1 (MQ=255) gagaACCCAGGGATGCAGGCTTCCGACATGGAGTTGGCCAATGCCATCCTGGCCAAGAATGCGGTAGCCGAGTAACTGCGTCGCCTGCTTCGCGGGTGAACCCGCTCCTACAGGCACCCGCGAAGCATCCAACACCAACCTCAGCGATaa < 2:194460/150‑1 (MQ=255) gaACCCAGGGATGCAGGCTTCCGACATGGAGTTGGCCAATGCCATCCTGGCCAAGAATGCGGTAGCCGAGTAACTGCGTCGCCTGCTTCGCGGGTGAACCCGCTCCTACAGGCACCCGCGAAGCATCCAACACCAACCTCAGCGATAAGc < 2:728510/150‑1 (MQ=255) ccAGGGATGCAGGCTTCCGACATGGAGTTGGCCAATGCCATCCTGGCCAAGAATGCGGTAGCCGAGTAACTGCGTCGCCTGCTTCGCGGGTGAACCCGCTCCTACAGGCACCCGCGAAGCATCCAACACCAACCTCAGCGATAAGCctct > 2:884339/1‑150 (MQ=255) aGGGATGCAGGCTTCCGACATGGAGTTGGCCAATGCCATCCTGGCCAAGAATGCGGTAGCCGAGTAACTGCGTCGCCTGCTTCGCGGGTGAACCCGCTCCTAc > 2:644182/1‑103 (MQ=255) aGGGATGCAGGCTTCCGACATGGAGTTGGCCAATGCCATCCTGGCCAAGAATGCGGTAGCCGAGTAACTGCGTCGCCTGCTTCGCGGGTGAACCCGCTCCTAc < 1:644182/103‑1 (MQ=255) gggATGCAGGCTTCCGACATGGAGTTGGCCAATGCCATCCTGGCCAAGAATGCGGTAGCCGAGTAACTGCGTCGCCTGCTTCGCGGGTGAACCCGCTCCTACAGGCACCCGCGAAGCATCCAACACCAACCTCAGCGATAAGCCTCTAcc < 1:389895/150‑1 (MQ=255) aTGCAGGCTTCCGACATGGAGTTGGCCAATGCCATCCTGGCCAAGAATGCGGTAGCCGAGTAACTGCGTCGCCTGCTTCGCGGGTGAACCCGCTCCTACAGGCACCCGCGAAGCATCCAACACCAACCTCAGCGATAAGCCTCTACCGGc > 1:887361/1‑150 (MQ=255) aGGCTTCCGACATGGAGTTGGCCAATGCCATCCTGGCCAAGAATGCGGTAGCCGAGTAACTGCGTCGCCTGCTTCGCGGGTGAACCCGCTCCTACAGGCACCCGCGAAGCATCCAACACCaa < 1:1249589/122‑1 (MQ=255) aGGCTTCCGACATGGAGTTGGCCAATGCCATCCTGGCCAAGAATGCGGTAGCCGAGTAACTGCGTCGCCTGCTTCGCGGGTGAACCCGCTCCTACAGGCACCCGCGAAGCATCCAACACCaa > 2:1249589/1‑122 (MQ=255) aGGCTTCCGACATGGAGTTGGCCAATGCCATCCTGGCCAAGAATGCGGTAGCCGAGTAACTGCGTCGCCTGCTTCGCGGGTGAACCCGCTCCTACAGGCACCCGCGAAGCATCCAACACCAAc > 2:1374608/1‑123 (MQ=255) aGGCTTCCGACATGGAGTTGGCCAATGCCATCCTGGCCAAGAATGCGGTAGCCGAGTAACTGCGTCGCCTGCTTCGCGGGTGAACCCGCTCCTACAGGCACCCGCGAAGCATCCAACACCAAc < 1:1374608/123‑1 (MQ=255) tCCGACATGGAGTTGGCCAATGCCATCCTGGCCAAGAATGCGGTAGCCGAGTAACTGCGTCGCCTGCTTCGCGGGTGAACCCGCTCCTACAGGCACCCGCGAAGCATCCAACACCAACCTCAGCGATAAGCCTCTACCGGCACACACCca < 2:644536/150‑1 (MQ=255) cGACATGGGGTTGGCCAATGCCATCCTGGCCAAGAATGCGGTAGCCGAGTAACTGCGTCGCCTGCTTCGCGGGTGAACCCGCTCCTACAGGCACCCGCGAAGCATCCAACACCAACCTCAGCGATAAGCCTCTACCGGCACACACGcaca < 2:710900/150‑1 (MQ=255) cGACATGGAGTTGGCCAATGCCATCCTGGCCAAGAATGCGGTAGCCGAGTAACTGCGTCGCCTGCTTCGCGGGTGAACCCGCTCCTACa > 2:558137/1‑89 (MQ=255) cGACATGGAGTTGGCCAATGCCATCCTGGCCAAGAATGCGGTAGCCGAGTAACTGCGTCGCCTGCTTCGCGGGTGAACCCGCTCCTACa < 1:558137/89‑1 (MQ=255) gggAGGTGGCCAATGCCATCCTGGCCAAGAATGCGGTAGCCGAGTAACTGCGTCGCCTGCTTCGCGGGTGAACCCGCTCCTACAGGCACCCGCGAAGCATCCAACACCAACCTCAGCGATAAGCCTCTACCGGCACACACGCACAGAACa > 2:844120/2‑150 (MQ=255) | TCGCGGGATCACAAGCTGGTAGTGGCCGCGCAGGATGACGCCAGCAGCTTCGTCGCCAGCGAAGGCGCGATCCGCGGGCCGTTCCTGGAAGCGGCCATGCGCGAGGCACGGGCAGAGAACCCAGGGATGCAGGCTTCCGACATGGAGTTGGCCAATGCCATCCTGGCCAAGAATGCGGTAGCCGAGTAACTGCGTCGCCTGCTTCGCGGGTGAACCCGCTCCTACAGGCACCCGCGAAGCATCCAACACCAACCTCAGCGATAAGCCTCTACCGGCACACACGCACAGAACA > NC_002947/5921359‑5921650 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |