Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,948,739 T→A 5.0% intergenic (‑64/+18) PP_mr62 ← / ← trxA Pseudomon‑Rho regulatory region/thioredoxin I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,948,7390TA 5.0% 251.8 / 3.9 80intergenic (‑64/+18)PP_mr62/trxAPseudomon‑Rho regulatory region/thioredoxin I
Reads supporting (aligned to +/- strand):  ref base T (39/36);  new base A (2/2);  total (42/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.20e-01

GCTGGATGGCGACAGGATCGCGTACTGATTCGAATTAGGGATGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTTGGTGGCTTCGACGTTGCCGTTCTTGAACAGCATCAGCGTCGGGATACCACGCACACCGTGCTTGGCCGGG  >  NC_002947/5948593‑5948883
                                                                                                                                                  |                                                                                                                                                
gcTGGATGGCGACAGGATCGCGTACTGATTCGAATTAGGGATGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGc                                                                                                                                               <  2:1364767/150‑1 (MQ=255)
   ggATGGCGACAGGATCGCGTACTGATTCGAATTAGGGATGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCggg                                                                                                                                            <  1:418065/150‑1 (MQ=255)
   ggATGGCGACAGGATCGCGTACTGATTCGAATTAGGGATGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCggg                                                                                                                                            <  2:1370990/150‑1 (MQ=255)
      tGGCGACAGGATCGCGTACTGATTCGAATTAGGGATGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGGGcc                                                                                                                                         >  1:40228/1‑150 (MQ=255)
      tGGCGACAGGATCGCGTACTGATTCGAATTAGGGATGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGGGcc                                                                                                                                         >  1:992987/1‑150 (MQ=255)
       ggCGACAGGATCGCGTACTGATTCGAATTAGGGACGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGGGCCt                                                                                                                                        <  2:1376933/150‑1 (MQ=255)
                       aCTGATTCGAATTAGGGATGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTg                                                                                                                        <  2:40228/150‑1 (MQ=255)
                         tGATTCGAATTAGGGATGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTggg                                                                                                                      >  2:1635184/1‑150 (MQ=255)
                         tGATCCGAATTAGGGATGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAATAAAAGCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTggg                                                                                                                      <  1:261612/150‑1 (MQ=255)
                           aTTCGAATTAGGGATGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGGGCCTTTtcac                                                                                                                                  <  1:1527200/136‑1 (MQ=255)
                           aTTCGAATTAGGGATGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGGGCCTTTtcac                                                                                                                                  >  2:1527200/1‑136 (MQ=255)
                             tCGAATTAGGGATGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTc                                                                                                                  <  1:308844/150‑1 (MQ=255)
                               gAATTAGGGATGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGa                                                                                                                >  1:257967/1‑150 (MQ=255)
                                  ttAGGGATGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCCTTTGCGGGGCCTTTTCACATCAAAAGTGGGCGTCTAGGa                                                                                                             >  2:370225/1‑150 (MQ=255)
                                  ttAGGGATGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGa                                                                                                             >  2:864872/1‑150 (MQ=255)
                                       gATGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCg                                                                                                        <  2:992987/150‑1 (MQ=255)
                                       gATGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCg                                                                                                        >  1:762375/1‑150 (MQ=255)
                                        aTGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCgg                                                                                                       >  2:173129/1‑150 (MQ=255)
                                        aTGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCgg                                                                                                       >  1:68326/1‑150 (MQ=255)
                                            ttCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGGGCCtttt                                                                                                                                     >  1:720128/1‑116 (MQ=255)
                                            ttCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGGGCCtttt                                                                                                                                     <  2:720128/116‑1 (MQ=255)
                                            ttCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAAAGTGCAACGCGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAg                                                                                                   >  2:1635607/1‑150 (MQ=255)
                                              cGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCt                                                                                                 <  2:1353653/150‑1 (MQ=255)
                                                     ggCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCAAATGCGGGGCtttt                                                                                                                                      <  2:1217154/106‑1 (MQ=255)
                                                     ggCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCAAATGCGGGGCtttt                                                                                                                                      >  1:1217154/1‑106 (MQ=255)
                                                         tGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAACGCGGCCAGCTGCGa                                                                                             >  2:1536891/1‑143 (MQ=255)
                                                         tGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAACGCGGCCAGCTGCGa                                                                                             <  1:1536891/143‑1 (MQ=255)
                                                          gTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACa                                                                                     >  2:1166626/1‑150 (MQ=255)
                                                             catggCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGTGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTg                                                                                  <  1:243808/147‑1 (MQ=255)
                                                              agGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTgc                                                                                 <  2:762375/150‑1 (MQ=255)
                                                                ggCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTgcgc                                                                               >  1:1452635/1‑150 (MQ=255)
                                                                  cACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTgg                                                                                                                       >  1:242099/1‑108 (MQ=255)
                                                                  cACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTgg                                                                                                                       <  2:242099/108‑1 (MQ=255)
                                                                         aaaGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTtgg                                                                      >  1:1614177/1‑150 (MQ=255)
                                                                          aaGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTtggt                                                                     <  1:864872/150‑1 (MQ=255)
                                                                          aaGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTtggt                                                                     <  1:1635184/150‑1 (MQ=255)
                                                                          aaGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTtggt                                                                     <  1:1359742/150‑1 (MQ=255)
                                                                           aGCAGTGCGAGGCCGAATGTAACACTTGATTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTtggtg                                                                    >  2:1253070/1‑150 (MQ=255)
                                                                                tGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGaa                                                                                                            >  2:1573827/1‑105 (MQ=255)
                                                                                tGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGaa                                                                                                            <  1:1573827/105‑1 (MQ=255)
                                                                                tGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTTGGTGGCTTc                                                               <  1:1166626/150‑1 (MQ=255)
                                                                                  cGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGc                                                                                                                                               <  1:982233/68‑1 (MQ=255)
                                                                                  cGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGc                                                                                                                                               >  2:982233/1‑68 (MQ=255)
                                                                                  cGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTTGGTGGCTTCGa                                                             <  2:489578/150‑1 (MQ=255)
                                                                                      gCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGGGCCTTTTCCCACCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTTGGTGGCTTCGACGtt                                                         >  2:132035/1‑150 (MQ=255)
                                                                                      gCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTAGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTTGGTGGCTTCGACGtt                                                         >  2:168776/1‑150 (MQ=255)
                                                                                          aaTGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAACAGCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTTGGTGGCTTCGACGTTGCCg                                                     <  1:370225/150‑1 (MQ=255)
                                                                                          aaTGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCtt                                                                        <  2:592051/131‑1 (MQ=255)
                                                                                          aaTGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCtt                                                                        >  1:592051/1‑131 (MQ=255)
                                                                                          aaTGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTTGGTGGCTTCGACGTTGCCg                                                     >  1:983470/1‑150 (MQ=255)
                                                                                          aaTGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTTGGTGGCTTCGACGTTGCCg                                                     >  1:62700/1‑150 (MQ=255)
                                                                                                    ttGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAg                                                                                                               >  2:57513/1‑82 (MQ=255)
                                                                                                    ttGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAg                                                                                                               <  1:57513/82‑1 (MQ=255)
                                                                                                      gCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTTGGTGGCTTCGACGTTGCCGTTCTTGAACAGc                                         <  2:412024/150‑1 (MQ=255)
                                                                                                        ttttttCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTTGGTGGCTTCGACGTTGCCGTTCTTGAACAGCAt                                       >  1:391884/1‑150 (MQ=255)
                                                                                                        ttttttCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTTGGTGGCTTCGACGTTGCCGTTCTTGAACAGCAt                                       <  2:391884/150‑1 (MQ=255)
                                                                                                        ttttttCTGCGTCTAGTGCTCTGAAAAAGAAAAGCACCGCACTTGAGGAGCTTTTTAACATCACAGCTGGGCGTCGATGAATGCGGCCAGCTGCGATTTGGACAGTGAGCCAACCTTGGTGGCTTCGACGTTGTCGTTCTTGAACGGCAt                                       >  2:57309/1‑150 (MQ=255)
                                                                                                                  gTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAg                                                                                                               >  1:1550692/1‑68 (MQ=255)
                                                                                                                  gTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAg                                                                                                               <  2:1550692/68‑1 (MQ=255)
                                                                                                                       gTGCTCTGAAAAAGAAAAGCCCCGCATGTGCGGGGCCTTTTCAAATCACAGGGGCGCGTCGAGGAATGGCGCCAGGTGCGACTTGGACAGAGCGCCAACGTTGGTGGCGTCGACATTGCCGTGGTTGAACAGCATCAGCGTCgggctgaa                        >  2:570193/1‑145 (MQ=255)
                                                                                                                        tGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTtggt                                                                     <  1:230826/104‑1 (MQ=255)
                                                                                                                        tGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTtggt                                                                     >  2:230826/1‑104 (MQ=255)
                                                                                                                          ctctGAAAAAGAAAAGCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTTGGTGGCTTCGACGTTGCCGTTc                                                  <  2:1520343/121‑1 (MQ=255)
                                                                                                                          ctctGAAAAAGAAAAGCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTTGGTGGCTTCGACGTTGCCGTTc                                                  >  1:1520343/1‑121 (MQ=255)
                                                                                                                           tctGAAAAAGAAAAGCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTTGGTGGCTTCGACGTTGCCGTTCTTGAACAGCATCAGCGTCGGGATACCACGc                    >  2:687333/1‑150 (MQ=255)
                                                                                                                              gAAAAAGAAAAGCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTTGGTGGCTTCGACGTTGCCGTTCTTGAACAGCATCAGCGTCGGGATACCACGcaca                 >  2:662288/1‑150 (MQ=255)
                                                                                                                               aaaaaGAAAAGCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTTGGTGGCTTCGACGTTGCCGTTCTTGAACAGCATCAGCGTCGGGATACCACGCacac                >  2:222647/1‑150 (MQ=255)
                                                                                                                                     aaaggCCCCGCAAATGCGGGGCTTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTTGGTGGCTTCGa                                                             >  1:1320013/1‑99 (MQ=38)
                                                                                                                                     aaaggCCCCGCAAATGCGGGGCTTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTTGGTGGCTTCGa                                                             <  2:1320013/95‑1 (MQ=38)
                                                                                                                                      aaagCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTAGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTTGGTGGCTTCGACGTTGCCGTTCTTGAACAGCATCAGCGTCGGGATACCACGCACACCGTGCtt         <  1:168776/150‑1 (MQ=255)
                                                                                                                                       aagCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTTGGTGGCTTCGACGTTGCCGTTCTTGAACAGCATCAGCGTCGGGATAATACGCACGCAGTGCGTg        >  2:116359/1‑150 (MQ=255)
                                                                                                                                        agCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTTGGTGGCTTCGACGTTGCCGTTCTTGAACAGCATCAGCGTCGGGATACCACGCACACCGTGCTTgg       <  2:257967/150‑1 (MQ=255)
                                                                                                                                            ccGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTTGGTGGCTTCGACGTTGCCGTTCTTGAACAGCAtaa                                     >  2:672644/1‑114 (MQ=255)
                                                                                                                                            ccGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTTGGTGGCTTCGACGTTGCCGTTCTTGAACAGCATCa                                     <  1:672644/116‑1 (MQ=255)
                                                                                                                                            ccGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTTGGTGGCTTCGACGTTGCCGTTCTTGAACAGCATCAGCGTCGGGATACCACGCACACCGTGCTTGGCCgg   >  1:1353121/1‑150 (MQ=255)
                                                                                                                                             cGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAAc                                                                           >  2:643496/1‑77 (MQ=255)
                                                                                                                                             cGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAAc                                                                           <  1:643496/77‑1 (MQ=255)
                                                                                                                                             cGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTTGGTGGCTTCGACGTTGCCGTTCTTGAACAGCATCAGCGTCGGGATACCACGCACACCGTGCTTGGCCggg  <  1:662288/150‑1 (MQ=255)
                                                                                                                                              gCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTtggt                                                                     >  2:1369462/1‑82 (MQ=255)
                                                                                                                                              gCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTtggt                                                                     <  1:1369462/82‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
GCTGGATGGCGACAGGATCGCGTACTGATTCGAATTAGGGATGCTTCGGCGACGGCATGTAGAGGGCACTGGAAAAGCAGTGCGAGGCCGAATGTAACACTTGCTTTTTTCTGCGTCTAGTGCTCTGAAAAAGAAAAGCCCCGCATTTGCGGGGCCTTTTCACATCACAGGTGGGCGTCGAGGAATGCGGCCAGCTGCGATTTGGACAGTGCGCCAACCTTGGTGGCTTCGACGTTGCCGTTCTTGAACAGCATCAGCGTCGGGATACCACGCACACCGTGCTTGGCCGGG  >  NC_002947/5948593‑5948883

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑