| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 473,350 | G→T | 9.1% | A546A (GCC→GCA) | dnaG ← | DNA primase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 473,350 | 0 | G | T | 9.1% | 162.4 / 3.0 | 53 | A546A (GCC→GCA) | dnaG | DNA primase |
| Reads supporting (aligned to +/- strand): ref base G (31/16); new base T (3/2); total (35/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGCCATTCCTTTTCCGCCAGGGCGCGTAGCAGGCGGCCCTGTTCGGTGCCATGCCAACGTGCGATCAACTGCATTGAGCTTAGCCCAGGATTTTTCTGCGCGGCTTCGATCAATGCCACCAGCAGCTGGCTGTACAGGTGTTCTTCGTCGGCGAAGTGGCTGGCATCTTCCACCTTGCCGGCCAGCAGTGGGTGGTGCAGCAGGGTGCGCAGGGCGGCCAGCGTGGGCGGCTCGACCGGCGCTGGCACGCGGGGAGGCGCTTCGTCGCGTTGCTGCCAGGGCTTGCCGCCCTTGCGG > NC_002947/473201‑473497 | aGCCATTCCTTTTCCGCCAGGGCGCGTAGCAGGCGGCCCTGTTCGGTGCCATGCCAACGTGCGATCAACTGCATTGAGCTTAGCCCAGGATTTTTCTGCGCGGCTTCGATCAATGCCACCAGCAGCTGGCTGTACAGGTGTTCTtcgtcg > 2:678566/1‑150 (MQ=255) aTTCCTTTTCCGCCAGGGCGCGTAGCAGGCGGCCCTGTTCGGTGCCATGCCAACGTGCGATCAACTGCATTGAGCTGAGCCCAGGATTTTTCTGCGCGGCTTCGATCAATGCCACCAGCAGCTGGCTGTACAGGTGTTCTTCGTCGGCGa > 1:927634/1‑150 (MQ=255) ttttCCGCCAGGGCGCGTAGCAGGCGTCCCTGTTCGGTGCCATGCCAACGTGCGATCAACTGCATTGAGCTTAGCCCAGGATTTTTCTGCGCGGCTTCGATCAATGCCACCAGCAGCTGGCTGTACAGGTGTTCTTCGTCGGCGAAGtgg > 2:1329803/1‑150 (MQ=255) ttttCCGCCAGGGCGCGTAGCAGGCGGCCCTGTTCGGTGCCATGCCAACGTGCGATCAACTGCATTGAGCTTAGCCCAGGATTTTTCTGCGCGGCTTCGATCAATGCCACCAGCAGCTGGCTGTACAGGTGTTCTTCGTCGGCGAAGtgg > 2:748470/1‑150 (MQ=255) ttttCCGCCAGGGCGCGTAGCAGGCGGCCCTGTTCGGTGCCATGCCAACGTGCGATCAACTGCATTGAGCTTAGCACAGGATTTTTCTGCGCGGCTTCGATCAATGCCACCAGCAGCTGGCTGTACAGGTGTTCTTCGTCGACGAAGtgg > 2:230730/1‑150 (MQ=255) accgaacAGGGCGCGTAGCAGGCGGCCCTGTTCGGTGCCATGCCAACGTGCGATCAACTGCATTGAGCTTAGCCCAGGATTTTTCTGCGCGGCTTCGATCAATGCCACCAGCAGCTGGCTGTACAGGTGTTCTTCGTCGGCGAAGtggct > 1:721200/7‑150 (MQ=255) tCCGCCAGGGCGCGTAGCAGGCGGCCCTGTTCGGTGCCATGCCAACGTGCGATCAACTGCATTGAGCTTAGCCCAGGATTTTTCTGCGCGGCTTCGATCAATGCCACCAGCAGCTGGCTGTACAGGTGTTCTTCGTCGGCGAAGtggctg < 1:748470/150‑1 (MQ=255) aGGGCGCGTAGCAGGCGGCCCTGTTCGGTGCCATGCCAACGTGCGATCAACTGCATTGAGCTTAGCCCAGGATTTTTCTGCGCGGCTTCGATCAATGCCACCAGCAGCTGGCTGTACAGGTGTTCTTCGTCGGCGAAGTGGCTGGCATCt < 2:721200/150‑1 (MQ=255) aGCAGGCGGCCCTGTTCGGTGCCATGCCAACGTGCGATCAACTGCATTGAGCTTAGCCCAGGATTTTTCTGCGCGGCTTCGATCAATGCCACCAGCAGCTGGCTGTACAGGTGTTCTTCGTCGGCGAAGTGGCTGGCATCTTCCa > 1:282233/1‑145 (MQ=255) aGCAGGCGGCCCTGTTCGGTGCCATGCCAACGTGCGATCAACTGCATTGAGCTTAGCCCAGGATTTTTCTGCGCGGCTTCGATCAATGCCACCAGCAGCTGGCTGTACAGGTGTTCTTCGTCGGCGAAGTGGCTGGCATCTTCCa < 2:282233/145‑1 (MQ=255) gCAGGCGGCCCTGTTCGGTGCCATGCCAACGTGCGATCAACTGCATTGAGCTTAGCCCAGGATTTTTCTGCGCGGCTTCGATCAATGCCACCAGCAGCTGGCTGTACAGGTGTTCTTCGTCGGCGAAGTGGCTGGCATCTTCCACCTTGc > 2:907450/1‑150 (MQ=255) gcggcCCTGTTCGGTGCCATGCCAACGTGCGATCAACTGCATTGAGCTTAGCCCAGGATTTTTCTGCGCGGCTTCGATCAATGCCACCAGCAGCTGGCTGTACAGGTGTTCTTCGTCGGCGAAGTGGCTGGCATCTTCCACCTTGCCGGc < 1:907450/150‑1 (MQ=255) gcggcCCTGTTCGGTGCCATGCCAACATCCAATAAACCAGCTCGAGGTCTATTTATTGTTTTTCTGCGATGCGGCGGTCAATGCCACCAGCAGCTGGCTGTACAGGTGTTCTTCGTCCGCGAAGTGGCTGGCATCTTACACCTTGCCGGc > 1:691975/1‑150 (MQ=255) cggcCCTGTTCGGTGCCATGCCAACGTGCGATCAACTGCATTGAGCTTAGCCCAGGATTTTTCTGCGCGGCTTCGATCAATGCCACCAGCAGCTGGCTGTACAGGTGTTCTTCGTCGGCGAAGTGGCTGGCATCTTCCACCTTGCCGGcc < 2:1117941/150‑1 (MQ=255) ggcCCTGTTCGGTGCCATGCCAACGTGCGATCAACTGCATTGAGCTTAGCCCAGGATTTTTCTGCGCGGCTTCGATCAATGCCACCAGCAGCTGGCTGTACAGGTGTTCTTCGGCGGCGAAGTGGCTGGCATCTTCCACCTTGCCGGCca < 2:1166226/150‑1 (MQ=255) cccTGTTCGGTGACATGCCAACGTGCGATCAACTGCATTGAGCTTAGCCCAGGATGTTTCTGCGCGGCTTCGATCAATGCCACCAGCAGCTGGCTGTACAGGTGTTCTTAATCGGCGAAGTGGCTGGCATCTTCCACCTTGCCGGCcagc > 2:569733/1‑150 (MQ=255) ccTGTTCGGTGCCATGCCAACGTGCGATCAACTGCATTGAGCTTAGCCCAGGATTTTTCTGCGCGGCTTCGATCAATGCCACCAGCAGCTGGCTGTACAGGTGTTCTTCGTCGGCGAAGTGGCTGGCATCTTCCACCTTGCCGGCcagca > 2:1451465/1‑150 (MQ=255) cGGTGCCATGCCAACGTGCGATCAACTGCATTGAGCTTAGCCCAGGATTTTTCTGCGCGGCTTCGATCAATGCCACCAGCAGCTGGCTGTACAGGTGTTCTTCGTCGGCGAAGTGGCTGGCATCTTCCACCTTGCCGGCCAGCAgtgggt > 1:437781/1‑150 (MQ=255) gccatgccaACGTGCGATCAACTGCATTGAGCTTAGCCCAGGATTTTTCTGCGCGGCTTCGATCAATGCCACCAGCAGCTGGCTGTACAGGTGTTCTTCGTCGGCGAAGTGGCTGGCATCTTCCACCTTGCCGGCCAGCAGTGGgtggtg < 2:437781/150‑1 (MQ=255) tGCGATCAACTGCATTGAGCTTAGCCCAGGATTTTTCTGCGCGGCTTCGATCAATGCCACCAGCAGCTGGCTGTACAGGTGTTCTTCGTCGGCGAAGTGGCTGGCATCTTCCACCTTGc > 2:1495534/1‑119 (MQ=255) tGCGATCAACTGCATTGAGCTTAGCCCAGGATTTTTCTGCGCGGCTTCGATCAATGCCACCAGCAGCTGGCTGTACAGGTGTTCTTCGTCGGCGAAGTGGCTGGCATCTTCCACCTTGc < 1:1495534/119‑1 (MQ=255) aTCAACTGCATTGAGCTTAGCCCAGGATTTTTCTGCGCGACTTCGAGCAAGGCCAGCAGCAGCTGGCTGTACAGGTGTTCTTCTTCGGCTAAGTGGCTGGCATCTTCCACCTTGCCGGCCAGCAGTGGGTGGTGCAGCAGGGTGCGCAgg > 2:1006915/1‑150 (MQ=255) tCAACTGCATTGAGCTTAGCCCAGGATTTTTCTGCGCGGCTTCGATCAATGCCACCAGCAGCTGGCTGTACAGGTGTTCTTCGTCGGCGAAGTGGCTGGCATCTTCCACCTTGCCGGCca < 1:1243246/120‑1 (MQ=255) tCAACTGCATTGAGCTTAGCCCAGGATTTTTCTGCGCGGCTTCGATCAATGCCACCAGCAGCTGGCTGTACAGGTGTTCTTCGTCGGCGAAGTGGCTGGCATCTTCCACCTTGCCGGCca > 2:1243246/1‑120 (MQ=255) cTGCATTGAGCTTAGCCCAGGATTTTTCTGCGCGGCTTCGATCAATGCCACCAGCAGCTGGCTGTACAGGTGTTCTTCGTCGGCGAAGTGGCTGGCATCTTCCACCTTGCCGGCCAGCAGTGGGTGGTGCAGCAGGGTGCGCAGggcggc > 2:965769/1‑150 (MQ=255) tGCATTGAGCTTAGCCCAGGATTTTTCTGCGCGGCTTCGATCAATGCCACCAGCCGCTGGATGTCCAGGTGTTCTTCGTCTGCCGAGTGGCTGTTATTTTTTACCTTGCCGGCCAGCAGTGGGTGGTGCAGCAGGGTGCGCAGGGCGGcc > 2:184105/1‑150 (MQ=255) tGCATTGAGCTTAGCCCAGGATTTTTCTGCGCGGCTTCGATCAATGCCACCAGCAGCTGGCTGTACAGGTGTTCTTCGTCGGCGAAGTGGCTGGCATCTTCCACCTTGCCGGCCAGCAGTGGGTGGTGCGGCAGGGTGCGCAGGGCGGcc > 2:1570579/1‑150 (MQ=255) cccAGGATTTTTCTGCGCGGCTTCGATCAATGCCACCAGCAGCTGGCTGTACAGGTGTTCTTCGTCGGCGAAGTGGCTGGCATCTTCCACCTTGCCGGCCAGCAGTGGGTAGTGCAGCAGGGTGCGCAGGGCGGCCAGCGTGGGCGGCTc > 1:830652/1‑150 (MQ=255) tCTGCGCGTCTTCGATCAATGCCCCGAGCAGTTGGCTGTACAGGTGTTCTTCGTCGGCGAAGTGGCTGGCATCTTCCACCTTGCCGGCCAGCAGTGGGTGGTGCAGCAGGGTGCGCAGGGCGGCCAGCGTGGGCGGCTCGACCGGCGCTg < 2:205747/150‑1 (MQ=255) gcgGCTTCGATCAATGCCACCAGCAGCTGGCTGTACAGGTGTTCTTCGTCTGCGAAGTGGCTGGCATCTTCCACCTTGCCGGCCAGCAGTGGGTGGTGCAGCAGGGTGCGCAGGGCGGCCAGCGTGGGCGGCTCGACCGGCGCTGGCAcg > 2:39128/1‑150 (MQ=255) gcgGCTTCGATCAATGCCACCAGCAGCTGGCTGTACAGGTGTTCTTCGTCTGCGAAGTGGCTGGCATCTTCCACCTTGCCGGCCAGCAGTGGGTGGTGCAGCAGGGTGCGCAGGGCGGCCAGCGTGGGCGGCTCGACCGGCGCTGGCAcg > 2:39027/1‑150 (MQ=255) cTTCGATCAATGCCACCAGCAGCTGGCTGTACAGGTGTTCTTCGTCGGCGAAGTGGCTGGCATCTTCCACCTTGCCGGCCAGCAGTGGGTGGTGCAGCAGGGTGCGCAGGGCGGCCAGCGTGGGCGGCTCGACCGGCGCTGGCACGCggg > 1:1109430/1‑150 (MQ=255) gATCAATGCCACCAGCAGCTGGCTGTACAGGTGTTCTTCGTCGGCGAAGTGGCTGGCATCTTCCACCTTGCCGGCCAGCAGTGGGTGGTGCAGCAGGGTGCTCAGGGCGGCCAGCGTGGGCGGCTCGACCGGCGCTGGCACGCGGGGAgg > 1:514047/1‑150 (MQ=255) gATCAATGCCACCAGCAGCTGGCTGTACAGGTGTTCTTCGTCGGCGAAGTGGCTGGCATCTTCCACCTTGCCGGCCAGCAGTGGGTGGTGCAGCAGGGTGCGCAGGGCGGCCAGCGTGGGCGGCTCGACCGGCGCTGGCACGCGGGGAgg < 2:1109430/150‑1 (MQ=255) aTCAATGCCACCAGCAGCTGGCTGTACAGGTATTCTTCGTCGGCGAAGTGGCTGGCATCTTCCACCTTGCCGGCCAGCAGTGGGTGGTGCAGCAGGGTGCGCAGGGCGGCCAGCGTGGGCGGCTCGACCGGCGCTGGCACGCGGGGAGgc > 2:106700/1‑150 (MQ=255) tGCCACCAGCAGCTGGCTGTACAGGTGTTCTTCGTCGGCGAAGTGGCTGGCATCTTCCACCTTGCCGGCCAGCAGTGGGTGGTGCAGCAGGGTGAGCAACTCTGCCAGCGTGGGCGGCTCGACCGGCGCTGGCACGCGGGGAGGCGCTtc > 1:1178660/1‑150 (MQ=255) caccaGCAGCTGGCTGTACAGGTGTTCTTCGTCGGCGAAGTGGCTGGCATCTTCCACCTTGCCGGCCAGCAGTGGGTGGTGCAGCAGGGTGCGCAGGGCGGCCAGCGTGGGCGGCTCGACCGGCGCTGGCACGCGGGGAGGCGCTtcgtc < 2:927634/150‑1 (MQ=255) caccaGCAGCTGGCTGTACAGGTGTTCTTCGTCGGCGAAGTGGCTGGCATCTTCCACCTTGCCGGCCAGCAGTGGGTGGTGCAGCAGGGTGCGCAGGGCGGCCAGCGTGGGCGGCTCGACCGGCGCTGGCACGCGGGGAGGCGCTtcgtc > 1:534332/1‑150 (MQ=255) caccaGCAGCTGGCTGTACAGGTGTTCTTCGTCGGCGAAGTGGCTGGCATCTTCCACCTTGCCGGCCAGCAGTGGGTGGTGCAGCAGGGTGCGCAGGGCGGCCAGCGTGGGCGGCTCGACCGGCGCTGGCACGCGGGGAGGCGCTtcgtc < 2:718637/150‑1 (MQ=255) caccaGCAGCTGGCTGTACAGGTGTTCTTCGTCGGCGAAGTGGCTGGCATCTTCCACCTTGCCGGCCAGCAGTGGGTGGTGCAGCAGGGTGCGCAGGGCGGCCAGCGTGGGCGGCTCGACCGGCGCTGGCACGCGGGGAGGCGCTtcgtc > 2:1041932/1‑150 (MQ=255) accaGCAGCTGGCTGTACAGGTGTTCTTCGTCGGCGAAGTGGCTGGCATCTTCCACCTTGCCGGCCAGCAGTGGGTGGTGCAGCAGGGTGCGCAGGGCGGCCAGCGTGGGCGGCt > 2:1534304/1‑115 (MQ=255) accaGCAGCTGGCTGTACAGGTGTTCTTCGTCGGCGAAGTGGCTGGCATCTTCCACCTTGCCGGCCAGCAGTGGGTGGTGCAGCAGGGTGCGCAGGGCGGCCAGCGTGGGCGGCt < 1:1534304/115‑1 (MQ=255) gTACAGGTGTTCTTCGTCGGCGAAGTGGCTGGCATCTTCCACCTTGCCGGCCAGCAGTGGGTGGTGCAGCAGGGTGCGCAGGGCGGCCAGCGTGGGCGGCTCGACCGGCGCTGGCACGCGGGGAGGCGCTTCGTCGCGTTGCTGCCAggg < 1:678566/150‑1 (MQ=255) aGGTGTTCTTCGTCGGCGAAGTGGCTGGCATCTTCCACCTTGCCGGCCAGCAGTGGGTGGTGCAGCAGGGTGCGCAGGGCGGCCAGCGTGGGCGGCTCGACCGGCGCTGGCACGCGGGGAGGCGCTTCGTCGCGTTGCTGCCAGGGCTTg > 1:329709/1‑150 (MQ=255) ggTGTTCTTCGTCGGCGAAGTGGCTGGCATCTTCCACCTTGCCGGCCAGCAGTGGGTGGTGCAGCAGGGTGCGCAGGGCGGCCAGCGTGGGCGGCTCGACCGGCGCTGGCACGCGGGGAGGCGCTTCGTCGCGTTGCTGCCAGGGCTTgc > 2:921308/1‑150 (MQ=255) gtgtTCTTCGTCTGCGAAGTGGCTGGCATCTTCCACCTTGCCGGCCAGCAGTGGGTGGTGCAGCAGGGTGCGCAGGGCGGCCAGCGTGGGCGGCTCGACCGGCGCTGGCACGCGGGGAGGCGCTTCGTCGCGTTGCTGCCAGGGCTTgcc < 1:39128/150‑1 (MQ=255) gtgtTCTTCGTCTGCGAAGTGGCTGGCATCTTCCACCTTGCCGGCCAGCAGTGGGTGGTGCAGCAGGGTGCGCAGGGCGGCCAGCGTGGGCGGCTCGACCGGCGCTGGCACGCGGGGAGGCGCTTCGTCGCGTTGCTGCCAGGGCTTgcc < 1:39027/150‑1 (MQ=255) gtgtTCTTCGTCGGCGAAGTGGCTGGCATCTTCCACCTTGCCGGCCAGCAGTGGGTGGTGCAGCAGGGTGCGCAGGGCGGCCa < 1:94941/83‑1 (MQ=255) gtgtTCTTCGTCGGCGAAGTGGCTGGCATCTTCCACCTTGCCGGCCAGCAGTGGGTGGTGCAGCAGGGTGCGCAGGGCGGCCa > 2:94941/1‑83 (MQ=255) gtgtTCTTCGTCGGCGAAGTGGCTGGCATCTTCCACCTTGCCGGCCAGCAGTGGGTGGTGCAGCAGGGTGCGCAGGGCGGCCAGCGTGGGCGGCTCGACCGGCGCTGGCACGCGGGGAGGCGCTTCGTCGCGTTGCTGCCAGGGCTTgcc > 2:329223/1‑150 (MQ=255) tgtTCTTCGTCGGCGAAGTGGCTGGCATCTTCCACCTTGCCGGCCAGCAGTGGGTGGTGCAGCAGGGTGCGCAGGGCGGCCAGCGTGGGCGGCTCGACCGGCGCTGGCACGCGGGGAGGCGCTTCGTCGCGTTGCTGCCAGGGCTTgccg > 2:1161106/1‑150 (MQ=255) tgtTCTTCGTCGGCGAAGTGGCTGGCATCTTCCACCTTGCCGGCCAGCAGTGGGTGGTGCAGCAGGGTGCGCAGGGCGGCCAGCGTGGGCGGCTCGACCGGCGCTGGCACGCGGGGAGGCGCTTCGTCGCGTTGCTGCCAGGGCTTgccg > 1:127232/1‑150 (MQ=255) ttcGTCGGCGAAGTGGCTGGCATCTTCCACCTTGCCGGCCAGCAGTGGGTGGTGCAGCAGGGTGCGCAGGGCGGCCAGCGTGGGCGGCTCGACCGGCGCTGGCACGCGGGGAGGCGCTTCGTCGCGTTGCTGCCAGGGCTTGCCGCCCtt > 2:1516957/1‑150 (MQ=255) ttcGTCGGCGAAGTGGCTGGCATCTTCCACCTTGCCGGCCAGCAGTGGGTGGTGCAGCAGGGTGCGCAGGGCGGCCAGCGTGGGCGGCTCGACCGGCGCTGGCACGCGGGGAGGCGCTTCGTCGCGTTGCTGCCAGGGCTTGCCGCCCtt > 2:803708/1‑150 (MQ=255) gtcgGCGAAGTGGCTGGCATCTTCCACCTTGCCGGCCAGCAGTGGGTGGTGCAGCAGGGTGCGCAGGGCGGCCAGCGTGGGCGGCTCGACCGGCGCTGGCACGCGGGGAGGCGCTTCGTCGCGTTGCTGCCAGGGCTTGCCGCCCTTGCg > 2:1566142/1‑150 (MQ=255) tcgGCGAAGTGGCTGGCATCTTCCACCTTGCCGGCCAGCAGTGGGTGGTGCAGCAGGGTGCGCAGGGCGGCCAGCGTGGGCGGCTCGACCGGCGCTGGCACGCGGGGAGGCGCTTCGTCGCGTTGCTGCCAGGGCTTGCCGCCCTTGCgg < 2:329709/150‑1 (MQ=255) tcgGCGAAGTGGCTGGCATCTTCCACCTTGCCGGCCAGCAGTGGGTGGTGCAGCAGGGTGCGCAGGGCGGCCAGCGTGGGCGGCTCGACCGGCGCTGGCACGCGGGGAGGCGCTTCGTCGCGTTGCTGCCAGGGCTTGCCGCCCTTGCgg < 1:1566142/150‑1 (MQ=255) ggCGAAGTGGCTGGCATCTTCCACCTTGCCGGCCAGCAGTGGGTGGTGCAGCAGGGTGCGCAGGGCGGcc < 2:926961/70‑1 (MQ=255) ggCGAAGTGGCTGGCATCTTCCACCTTGCCGGCCAGCAGTGGGTGGTGCAGCAGGGTGCGCAGGGCGGcc > 1:926961/1‑70 (MQ=255) | AGCCATTCCTTTTCCGCCAGGGCGCGTAGCAGGCGGCCCTGTTCGGTGCCATGCCAACGTGCGATCAACTGCATTGAGCTTAGCCCAGGATTTTTCTGCGCGGCTTCGATCAATGCCACCAGCAGCTGGCTGTACAGGTGTTCTTCGTCGGCGAAGTGGCTGGCATCTTCCACCTTGCCGGCCAGCAGTGGGTGGTGCAGCAGGGTGCGCAGGGCGGCCAGCGTGGGCGGCTCGACCGGCGCTGGCACGCGGGGAGGCGCTTCGTCGCGTTGCTGCCAGGGCTTGCCGCCCTTGCGG > NC_002947/473201‑473497 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |