| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 6,039,292 | C→T | 10.5% | intergenic (‑256/+38) | PP_5290 ← / ← pyrE | transposase/orotate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 6,039,292 | 0 | C | T | 10.5% | 150.6 / 5.5 | 55 | intergenic (‑256/+38) | PP_5290/pyrE | transposase/orotate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (23/26); new base T (4/2); total (27/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.22e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAAAGGAGGAGGGGTGATTTCTCCTACGGTCTGTACTGCGCGAACAGTCAGAAGCGAGTTATGGCCCTCCTCCTCCCTTCAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCAC‑AAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGTTTCAGCTGTGGATCGTCAGCCAGGAACTCCAGCACCTGGGTCAGCGAAACAATGCT > NC_002947/6039145‑6039434 | gAAAGGAGGAGGGGTGATTTCTCCTACGGTCTGTACTGCGCGAACAGTCAGAAGCGAGTTATGGCCCTCCTCCTCCCTTCAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCAC‑AAAAAAAGGCGACCctc < 1:227929/150‑1 (MQ=255) aaGGAGGAGGGGTGATTTCTCCTACGGTCTGTACTGCGTGAACAGTCAGAAGCGAGTTATGGCCCTCCTCCTCCCCTCAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCAC‑AAAAAAAGGCGACCCTCTg < 1:1321511/150‑1 (MQ=255) cTACGGTCTGTACTGCGCGAACAGTCAGAAGCGAGTTATGGCCCTCCTCCTCCCTTCAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCAC‑AAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCAtt < 1:447329/150‑1 (MQ=255) gTCTGTACTGCGCGAACAGTCAGAAGCGAGTTATGGCCCTCCTCCTCCCTTCAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCAC‑AAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTgg < 1:417996/150‑1 (MQ=255) gTCTGTACTGCGCGAACAGTCAGAAGCGAGTTATGGCCCTCCTCCTCCCTTCAAGTCCTACCATACAAGCGGGTTTAAACGCGAAAGGGCAAGATTAGGGGCGAG‑AAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTgg < 1:692568/150‑1 (MQ=18) gTACTGCGCGAACAGTCAGAAGCGAGTTATGGCCCTCCTCCTCCCTTCAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCAC‑AAAAAAAGGCGACCTTCTTCAGAGGGTCGc < 2:451569/131‑1 (MQ=18) gTACTGCGCGAACAGTCAGAAGCGAGGTATGGCCCTCCTCCTCCCTTCAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCAC‑AAAAAAAGGCGACCTTCTTCAGAGGGTCGc > 1:451569/1‑131 (MQ=18) tGCGCGAACAGTCAGAAGCGAGTTATGGCCCTCCTCCTCCCTTCAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCAC‑AAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGAt > 2:945892/1‑150 (MQ=255) cgcgAACAGTCAGAAGCGAGTTATGGCCCTCCTCCTCCCTTCAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCACAAAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATc < 2:857623/150‑1 (MQ=255) cgAACAGTCAGAAGCGAGTTATGGCCCTCCTCCTCCCTTCAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCAC‑AAAAAAAAGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCATGAGCCAGGATCAGa > 1:334783/1‑150 (MQ=255) taaGAAGCGAGTTATGGCCCTCCTCCTCCCTTCAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCAC‑AAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTa > 2:1556740/3‑150 (MQ=255) gAGTTATGGCCCTCCTCCTCCCTTCAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCAC‑AAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGccc > 1:439451/1‑150 (MQ=255) gTTATGGCCCTCCTCCTCCCTTCAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCAC‑AAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCgg > 2:333057/1‑150 (MQ=255) aTGGCCCTCCTCCTCCCTTCAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCAC‑AAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAg < 1:385033/150‑1 (MQ=255) cccTCCTCCTCCCTTCAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCAC‑AAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCt < 1:1002488/150‑1 (MQ=255) cctcctcctCCCTTCAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCAC‑AAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTc > 2:979118/1‑150 (MQ=255) cctcctcctCCCTTCAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCAC‑AAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTc > 1:1543299/1‑150 (MQ=255) tcctcctccCTTCAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCAC‑AAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGa > 2:460856/1‑150 (MQ=255) ctccCTTCAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCAC‑AAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTg < 2:1047875/105‑1 (MQ=255) ctccCTTCAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCAC‑AAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTg > 1:1047875/1‑105 (MQ=255) cTTCAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCAC‑AAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTGTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCGCGGTAGGCCTCGACAGCCGGCa > 1:21826/1‑150 (MQ=255) aGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCCGAAACGGCGCTTG‑AAAGGAAAGCGAGCTTCTGAATAAGGTCGCCTTTTTCATTCCTGGGCAAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGc > 1:31022/1‑150 (MQ=255) aGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCAC‑AAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGc > 1:1220626/1‑150 (MQ=255) tCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCAC‑AAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCAt > 2:1007732/1‑89 (MQ=255) tCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCAC‑AAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCAt < 1:1007732/89‑1 (MQ=255) ccTACCATACAAGCGGGTTAAGCCGCGAAAGGGCCAGATCAGGCGGCAC‑AAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGt < 2:1613712/150‑1 (MQ=255) tACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCAC‑AAAAAAAGGCGACCCTCTGaagaag < 1:15128/72‑1 (MQ=255) tACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCAC‑AAAAAAAGGCGACCCTCTGaagaag > 2:15128/1‑72 (MQ=255) aCCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCAC‑AAAAAAAGGCGACCCTCTGAAGAAGGTCGCCttttt < 1:480659/82‑1 (MQ=255) aCCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCAC‑AAAAAAAGGCGACCCTCTGAAGAAGGTCGCCttttt > 2:480659/1‑82 (MQ=255) aaGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCAC‑AAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGTTTCAGCtgtg > 1:1540781/1‑150 (MQ=255) aGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCAC‑AAAAAAAGGCGACCTTCTTCAGAGGGTCGCCttt > 1:274440/1‑72 (MQ=21) aGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCAC‑AAAAAAAGGCGACCTTCTTCAGAGGGTCGCCttt < 2:274440/72‑1 (MQ=25) cGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCAC‑AAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGTTTCAGCTGTGGAt < 1:945892/150‑1 (MQ=255) gggTTTACCCGCGAAAGGGCCAGATCAGGCGGCAC‑AAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGTTTCAGCTGTGGATc < 2:850438/150‑1 (MQ=255) aCCCGCGAAAGGGCCAGATCAGGCGGCAC‑AAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGTTTCAGCTGTGGATCGTCAGc < 1:460856/150‑1 (MQ=255) cccGCGAAAGGGCCAGATCAGGCGGCAC‑AAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGTTTCAGCTGTGGATCGTCAGcc > 2:46447/1‑150 (MQ=255) gcgAAAGGGCCAGATCAGGCGGCAC‑AAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGTTTCAGCTGTGGATCGTCAGCCAgg > 2:45433/1‑150 (MQ=255) gcgAAAGGGCCAGATCAGGCGGCAC‑AAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGTTTCAGCTGTGGATCGTCAGCCAgg > 2:1339557/1‑150 (MQ=255) gAAAGGGCCAGATCAGGCGGCAC‑AAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGTTTCAGCTGTGGATCGTCAGCCAGGaa < 2:1543299/150‑1 (MQ=255) gAAAGGGCCAGATCAGGCGGCAC‑AAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGTTTCAGCTGTGGATCGTCAGCCAGGaa < 1:358521/150‑1 (MQ=255) aGGGCCAGATCAGGCGGCAC‑AAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTAATGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCGGTTTCAGCTGTGGATCGTCAGGCAGGAACTc < 2:495349/150‑1 (MQ=255) gCCAGATCAGGCGGCAC‑AAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGTTTCAGCTGTGGATCGTCAGCCAGGAACTCCAg > 2:1536347/1‑150 (MQ=255) cAGGCGGCAC‑AAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGTTTCAGCTGTGGATCGTCAGCCAGGAACTCCAGCACCTgg < 1:333057/150‑1 (MQ=255) gcAC‑AAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGTTTCAGCTGTGAATCGTCAGCCAGGAACTCCAGCACCTGGGTCAGc < 2:1220626/150‑1 (MQ=255) gaatgaaaaaGGCGACCTTCTTCAGAGGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGTTTCAGCTGTGGATCGTCAGCCAGGAACTCCAGCACCTGGGTCAGCg > 2:1355422/6‑150 (MQ=38) ac‑aAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGTTTCAGCTGTGGATCGTCAGCCAGGAACTCCAGCACCTGGGTCAGCGa > 1:643444/1‑150 (MQ=255) ac‑aAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGTTTCAGCTGTGGATCGTCAGCCAGGAACTACAGCCCCTGGATcggaga > 2:1168859/1‑145 (MQ=255) aaaaaaaGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGTTTCAGCTGTGGATCGTCAGCCAGGAACTCCAGCACCTGGGTCAGCGaaa < 1:979118/150‑1 (MQ=255) aaaaaaaGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGTTTCAGCTGTGGATCGTCAGCCAGGAACTCCAGCACCTGGGTCAGCGaaa < 1:1556740/150‑1 (MQ=255) aaGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGTTTCAGCTGTGGATCGTCAGCCAGGAACTCCAGCACCTGGGTCAGCGAAACAATg < 2:111641/150‑1 (MQ=255) aaGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGTTTCAGCTGTGGATCGTCAGCCAGGAACTCCAGCACCTGGGTCAGCGAAACAATg < 2:439451/150‑1 (MQ=255) ggCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTTGGCCTCGACAGCCGGCAGATGCTGTTTCa > 1:715359/1‑98 (MQ=255) ggCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGTTTCa < 2:715359/98‑1 (MQ=255) ggCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGTTTCAGCTGTGGATCGTCAGCCAGGAACTCCAGCACCTGGGTCAGCGAAACAATGCt > 1:782761/1‑150 (MQ=255) ggCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGTTTCAGCTGTGGATCGTCAGCCAGGAACTCCAGCACCTGGGTCAGCGAAACAATGCt < 1:1536347/150‑1 (MQ=255) ctctGAAGAAGGTCTCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGTTTCAGCTGTGGATCGTCAGCCAGGAACTCCAGCACCTGGGTCAGCGAAACAATGc < 2:1326883/142‑1 (MQ=255) ctctGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGTTTCAGCTGTGGATCGTCAGCCAGGAACTCCAGCACCTGGGTCAGCGAAACAATGc > 1:1326883/1‑142 (MQ=255) | GAAAGGAGGAGGGGTGATTTCTCCTACGGTCTGTACTGCGCGAACAGTCAGAAGCGAGTTATGGCCCTCCTCCTCCCTTCAAGTCCTACCATACAAGCGGGTTTACCCGCGAAAGGGCCAGATCAGGCGGCAC‑AAAAAAAGGCGACCCTCTGAAGAAGGTCGCCTTTTTCATTCCTGGGCCAGGATCAGATCCCGTACTGCGCCCGGTAGGCCTCGACAGCCGGCAGATGCTGTTTCAGCTGTGGATCGTCAGCCAGGAACTCCAGCACCTGGGTCAGCGAAACAATGCT > NC_002947/6039145‑6039434 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |