Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 536,392 T→G 8.7% noncoding (69/190 nt) PP_mr06 ← P27 ncRNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947536,3920TG 8.7% 122.9 / 5.5 47noncoding (69/190 nt)PP_mr06P27 ncRNA
Reads supporting (aligned to +/- strand):  ref base T (23/19);  new base G (2/2);  total (26/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

TCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACTCGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGTCC  >  NC_002947/536243‑536533
                                                                                                                                                     |                                                                                                                                             
tCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGt                                                                                                                                               <  2:1506317/150‑1 (MQ=255)
        cGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAAt                                                                                                                                       <  1:1630372/150‑1 (MQ=255)
         gCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATc                                                                                                                                      >  1:380114/1‑150 (MQ=255)
                                         gTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCg                                                                                                                  <  1:1149941/138‑1 (MQ=255)
                                         gTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCg                                                                                                                  >  2:1149941/1‑138 (MQ=255)
                                          tCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGCCTCGGCCAGAt                                                                                                     <  1:864356/150‑1 (MQ=255)
                                              gccgccAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGCCTCGGCCAGATGaaa                                                                                                 <  2:867839/150‑1 (MQ=255)
                                                  ccAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGGTAACGAATcggga                                                                                                                                  >  1:1246299/1‑109 (MQ=255)
                                                  ccAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGGTAACGAATcggga                                                                                                                                  <  2:1246299/113‑5 (MQ=255)
                                                    aGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTaa                                                                                           >  2:40498/1‑150 (MQ=255)
                                                       aaaaaTTGGGGTCCCTGCCTGGCGGGGGCTATCAAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTaaaaa                                                                                        >  1:962975/1‑150 (MQ=255)
                                                        aaaaTTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAAc                                                                                       >  2:299156/1‑150 (MQ=255)
                                                          aaTTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCa                                                                                     <  2:1449349/150‑1 (MQ=255)
                                                             tGGGGTCCCCGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGa                                                                                  <  1:1303945/150‑1 (MQ=255)
                                                                    cctgcctgGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACAGCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAAcc                                                                           >  1:1569566/1‑150 (MQ=255)
                                                                       gcctgGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGt                                                                        <  1:313991/150‑1 (MQ=255)
                                                                        cctgGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTg                                                                       >  1:278450/1‑150 (MQ=255)
                                                                        cctgGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTg                                                                       <  2:380114/150‑1 (MQ=255)
                                                                                 ggCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGt                                                                        <  1:1394240/140‑1 (MQ=255)
                                                                                 ggCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGt                                                                        >  2:1394240/1‑140 (MQ=255)
                                                                                 ggCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAAt                                                                  >  2:357091/1‑146 (MQ=255)
                                                                                 ggCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAAt                                                                  <  1:357091/146‑1 (MQ=255)
                                                                                   cTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAACACCAGGAGTAAACCCGTGGCAATTAAACt                                                            <  1:299156/150‑1 (MQ=255)
                                                                                   cTATCAAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACt                                                            <  2:962975/150‑1 (MQ=255)
                                                                                         aaGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGCCTCGGCCAGAAAAAAAAAAAAAAACCAGGAGTAAACCCGTGGCAATTAAACTCGAAGa                                                      <  2:1459186/150‑1 (MQ=255)
                                                                                          aGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACTCGAAGAc                                                     <  1:40498/150‑1 (MQ=255)
                                                                                           gACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTGCGGGCTCGCTACCGAATCAGACACCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACTCGAAGACa                                                    >  2:1636007/1‑150 (MQ=255)
                                                                                                tAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACTCGAAGACaagaag                                               <  2:1569566/150‑1 (MQ=255)
                                                                                                  ggcggcGCAAGCCTTAAACCATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGCCTCGGCCAGa                                                                                                      <  2:60772/93‑1 (MQ=255)
                                                                                                  ggcggcGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGCCTCGGCCAGa                                                                                                      >  1:60772/1‑93 (MQ=255)
                                                                                                        gcAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACTCGAAGACAAGAAGGCCAtcgt                                       >  1:279780/1‑150 (MQ=255)
                                                                                                        gcAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACTCGAAGACAAAAAGGCCAtcgg                                       >  2:910165/1‑149 (MQ=255)
                                                                                                               ttAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGCCATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACTCGAAGACAAGAAGGCCATCGTCGCTGaa                                >  2:1070305/1‑150 (MQ=255)
                                                                                                                tAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACTCGACGACAAGAAGCCCATCGTCGCTGAAg                               >  1:1068706/1‑150 (MQ=255)
                                                                                                                        cAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTCGCAATTAAACTCGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAg                       >  2:514363/1‑150 (MQ=255)
                                                                                                                         aGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACTCGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAgg                      <  2:279780/150‑1 (MQ=255)
                                                                                                                                          cTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACTCGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGctata     >  1:1157307/1‑147 (MQ=255)
                                                                                                                                           tCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACTCGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGt    >  1:796863/1‑150 (MQ=255)
                                                                                                                                           tCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACTCGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGt    >  1:278680/1‑150 (MQ=255)
                                                                                                                                           tCCCGATTAGTTACCGAATCAGACACCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAATAAACACTTGGCAATTAAACTCGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAAGCTGCCAAAGTAGctcagg    >  2:242386/1‑147 (MQ=255)
                                                                                                                                            tctgATTCGGTAACGAATCGGACACCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAg                                                                                    <  1:514721/66‑1 (MQ=37)
                                                                                                                                            tctgATTCGGTAACGAATCGGACACCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAg                                                                                    >  2:514721/4‑69 (MQ=37)
                                                                                                                                             ccGATTCGTTACCGAATCAGACACCAAAACGACATCCGGCCTCGGCCAGATGAAAc                                                                                                <  2:1319896/56‑1 (MQ=255)
                                                                                                                                             ccGATTCGTTACCGAATCAGACACCAAAACGACATCCGGCCTCGGCCAGATGAAAc                                                                                                >  1:1319896/1‑56 (MQ=255)
                                                                                                                                             ccGATTCGTTACCGAATCAGACACCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACTCGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGTcc  >  2:1340212/1‑150 (MQ=255)
                                                                                                                                             ccGATTCGTTACCGAATCAGACACCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACTCGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGTcc  >  2:991515/1‑150 (MQ=255)
                                                                                                                                                 ttCGTTACCGAATCAGACACCAAAACGACATCCGGc                                                                                                                <  2:589111/36‑1 (MQ=255)
                                                                                                                                                 ttCGTTACCGAATCAGACACCAAAACGACATCCGGc                                                                                                                >  1:589111/1‑36 (MQ=255)
                                                                                                                                                     |                                                                                                                                             
TCAGAGCTCGCTGAACGTGTAAGAACATGGCGGCGCGACCAGTCGCGCCGCCAGCAAAAATTGGGGTCCCTGCCTGGCGGGGGCTATCCAAGACCGTAGGCGGCGCAAGCCTTAAAACATCAGCCCACGCAGATGGTGCTCCCGATTCGTTACCGAATCAGACACCAAAACGACATCCGGCCTCGGCCAGATGAAACGGTAAAAACCAGGAGTAAACCCGTGGCAATTAAACTCGAAGACAAGAAGGCCATCGTCGCTGAAGTCAACGAGGCTGCCAAAGTCGCTCTGTCC  >  NC_002947/536243‑536533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑