| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 591,706 | C→G | 10.9% | intergenic (+17/+14) | PP_0501 → / ← PP_0502 | NAD‑dependent epimerase/dehydratase family protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 591,706 | 0 | C | G | 10.9% | 128.3 / 6.4 | 44 | intergenic (+17/+14) | PP_0501/PP_0502 | NAD‑dependent epimerase/dehydratase family protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (19/20); new base G (2/3); total (21/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGCTATGCGGCGGCGCGATCGGGTGATATCCGCCATTCGCGAGCAGATAACCAGCGGCTGTTGGCGCGGTTCGAGTTTGCGCAGGTGACGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGATGTAAGACCCGCCCATCCTGGCCACGGACCTGCAATATTTCAAGCGCCTGCCCCGTCACGCCGTCCACCAGTTCCGAACGCGCTTCTCCCGCCTC > NC_002947/591558‑591854 | aGCTATGCGGCGGCGCGATCGGGTGATATCCGCCATTCGCGAGCAGATAACCAGCGGCTGTTGGCGCGGTTCGAGTTTGCGCAGGTGACGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTca < 2:664245/150‑1 (MQ=255) gCTATGCGGCGTCGCGATGGGGTGATATCCTCGATTCGCGAGCAGATAACCGGCGGCTGTTGGCGCGGTTCGAGTTTGCCCAGGTGACGCTGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTcag < 2:173553/150‑1 (MQ=255) aTGCGGCGGCGCGATCGGGTGATATCCGCCATTCGCGAGCAGATAACCAGCGGCTGTTGGCGCGGTTCGAGTTTGCGCAGGTGACGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTcagcag > 1:1279464/1‑150 (MQ=255) aTGCGGCGGCGCGATCGGGTGATATCCGCCATTCGCGAGCAGATAACCAGCGGCTGTTGGCGCGGTTCGAGTTTGCGCAGGTGACGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTcagcag > 1:1244366/1‑150 (MQ=255) ggcggcgCGATCGGGTGATATCCGCCATTCGCGAGCAGATAACCAGCGGCTGTTGGCGCGGTTCGAGTTTGCGCAGGTGACGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCt < 2:1244366/150‑1 (MQ=255) ggcggcgCGATCGGGTGATATCCGCCATTCGCGAGCAGATAACCAGCGGCTGTTGGCGCGGTTCGAGTTTGCGCAGGTGACGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCt < 2:1279464/150‑1 (MQ=255) ggcgCGATCGGGTGATATCCGCCATTCGCGAGCAGATAACCAGCGGCTGTTGGCGCGGTTCGAGTTTGCGCAGGTGACGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCAGGGCTGCTGAGCAGccccgcg < 1:179475/150‑5 (MQ=255) gggTGATATCCGCCATTCGCGAGCAGATAACCAGCGGCTGTTGGCGCGGTTCGAGTTTGCGCAGGTGACGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCAt > 1:1104935/1‑150 (MQ=255) tCCGCCATTCGCGAGCAGATAACCAGCGGCTGTTGGCGCGGTTCGAGTTTGCGCAGGTGACGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCAcc > 1:1564654/1‑150 (MQ=255) tCCGCCATTCGCGAGCAGATAACCAGCGGCTGTTGGCGCGGTTCGAGTTTGCGCAGGTGACGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCAcc < 2:1599600/150‑1 (MQ=255) tCCGCCATTCGCGAGCAGATAACCAGCGGCTGTTGGCGCGGTTCGAGTTTGCGCAGGTGACGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCAcc > 1:334439/1‑150 (MQ=255) gCCATTCGCGAGCAGATAACCAGCGGCTGTTGGCGCGGTTCGAGTTTGCGCAGGTGACGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCtt < 2:970210/150‑1 (MQ=255) cgAGCAGATAACCAGCGGCTGTTGGCGCGGTTCGAGTTTGCGCAGGTGACGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTg < 2:334439/150‑1 (MQ=255) gAGCAGATAACCAGCGGCTGTTGGCGCGGTTCGAGTTTGCGCAGGTTACGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCGCGCGAGGCTGCTCAGCCGCCCTGACATCACCCATGCCGCACCCTTCTGCGCttg > 2:258140/1‑148 (MQ=255) tAACCAGCGGCTGTTGGCGCGGTTCGAGTTTGCGCAGGTGACGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCtt > 2:1112954/1‑150 (MQ=255) aaCCAGCGGCTGTTGGCGCGGTTCGAGTTTGCGCAGGTGACGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGc < 1:774031/124‑1 (MQ=255) aaCCAGCGGCTGTTGGCGCGGTTCGAGTTTGCGCAGGTGACGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGc > 2:774031/1‑124 (MQ=255) aCCGGCGGCTGTGGGCGCGGTTCGAGTTTGCGCAGGTGACGCCGATGGTCGAAGGCCTCACAAAGCTTCTCGGTAAAGGCCGAGCCCCGCGGTGCTGCTCAGCAGCCCTGCCATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCg < 2:88241/150‑1 (MQ=255) aCCAGCGGCTGTTGGCGCGGTTCGAGTTTGCGCAGGTGACGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTg > 2:245179/1‑140 (MQ=255) aCCAGCGGCTGTTGGCGCGGTTCGAGTTTGCGCAGGTGACGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTg < 1:245179/140‑1 (MQ=255) aCCAGCGGCTGTTGGCGCGGTTCGAGTTTGCGCAGGTGACGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCg < 1:1043404/150‑1 (MQ=255) ccAGCGGCTGTTGGCGCGGTTCGAGTTTGCGCAGGTGACGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCgg < 2:1104935/150‑1 (MQ=255) ggCTGTTGGCGCGGTTCGAGTTTGCGCAGGTGACGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGAt > 1:1346346/1‑148 (MQ=255) ggCTGTTGGCGCGGTTCGAGTTTGCGCAGGTGACGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGAt < 2:1346346/148‑1 (MQ=255) gTTGGCGCGGTTCGAGTTTGCGCAGGTGACGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGATGTAAGa < 1:1577848/150‑1 (MQ=255) gcgGTTCGAGTTTGCGCAGGTGACGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCt > 1:1025775/1‑93 (MQ=255) gcgGTTCGAGTTTGCGCAGGTGACGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCt < 2:1025775/93‑1 (MQ=255) gcgGTTCCAGCTTGCGCAGGTGACGCCGATGGTTGAAGGCCTGACAAATCTGCTCGGTAAAGGCTGAGCCCCGCGGCGCTGCTGAGCAGCCCTGACATCGCCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGTATGTAAGACCCGcc < 2:403412/150‑1 (MQ=255) aCGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGATGTAAGACCCGCCCATCCTGGCCACGGACCTGCaa < 1:1112954/150‑1 (MQ=255) gCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGATGTAAGACCCGCCCATCCTGGCCACGGACCTGCAata > 1:567058/1‑150 (MQ=255) gATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGATGTAAGACCCGCCCATCCTGGCCACGGACCTGCAATAttt < 2:1564654/150‑1 (MQ=255) ggTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGATGTAAGACCCGCCCATCCTGGCCACGGACCTGCAATATTTCaa < 2:567058/150‑1 (MQ=255) ccTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCAACCTTCTGCGCTGGCAATCTTCGGGATGTAAGACCCGCCCATCCTGGCCACGGACCTGCAATATTTCAAGCGCCTGcc < 1:258140/150‑1 (MQ=255) aCAAAGCTTCTCGGTAAAGGCTGAGCCACGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGATGTAAGACCCGCCCATCCTGGCCACGGACCTGCAATATTTCAAGCGCCTGCCCCGt > 1:784674/1‑150 (MQ=255) aaaGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCgg > 2:1163246/1‑90 (MQ=255) aaaGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCgg < 1:1163246/90‑1 (MQ=255) aaGCTTCTCGGTAAAGGCTGAGCCACGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGATGTAAGACCCGCCCATCCTGGCCACGGACCTGCAATATTTCAAGCGCCTGCCCCGTCAc < 2:784674/150‑1 (MQ=255) ttCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGATGTAAGACCCGCCCATCCTGGCCACGGACCTGCAATATTTCAAGCGCCTGCCCCGTCACGCCg > 2:345041/1‑150 (MQ=255) tAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGATGTAAGACCCGCCCATCCTGGCCACGGACCTGCAATATTTCAAGCGCCTGCCCCGTCACGCCGTccacca > 2:613680/1‑150 (MQ=255) ccGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGAt > 2:592992/1‑68 (MQ=255) ccGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGAt < 1:592992/68‑1 (MQ=255) ccGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGATGTAAGACCCGCCCATCCTGGCCACGGACCTGCAATATTTCAAGCGCCTGCCCCGTCACGCCGTCCACCAGTTCCGAACgcgc > 1:840199/1‑150 (MQ=255) tcagggCTGCTGAGCAGCCCCGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGATGTAAGACCCGCCCATCCTGGCCACGGACCTGCAATATTTCAAGCGCCTGCCCCGTCACGcc < 1:164342/124‑1 (MQ=255) tcagggCTGCTGAGCAGCCCCGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGATGTAAGACCCGCCCATCCTGGCCACGGACCTGCAATATTTCAAGCGCCTGCCCCGTCACGcc > 2:164342/4‑127 (MQ=255) agggCTGCTGAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGATGTAAGACCCGCCCATCCTGGCCACGGACCTGCAATATTTCAAGCGCCTGCCCCGTCACGCCGTCCACCAGTTCCGAACGCGCTtct > 2:980889/2‑150 (MQ=255) gctgctCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGATGTAAGACCCGCCCATCCTGGCCACGGACCTGCAATATTTCAAGCGCCTGCCCCGTCACGCCGTCCACCAGTTCCGAACGCGCTTCTccc > 1:1041162/1‑150 (MQ=255) tCAGCAGCCCTTACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGATGTAAGACCCGCCCATCCTGGCCACGGACCTGCAATATTTCAAGCGCCTGCCCCGTCACGCCGTCCACCAGTTCCGAACGCGCTTCTCCCGCCTc > 2:1510510/1‑150 (MQ=255) tCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGATGTAAGACCCGCCCATCCTGGCCACGGACCTGCAATATTTCAAGCGCCTGCCCCGTCACGCCGTCCACCAGTTCCGAACGCGCTTCTCCCGCCTc > 2:72920/1‑150 (MQ=255) | AGCTATGCGGCGGCGCGATCGGGTGATATCCGCCATTCGCGAGCAGATAACCAGCGGCTGTTGGCGCGGTTCGAGTTTGCGCAGGTGACGCCGATGGTTGAAGGCCTGACAAAGCTTCTCGGTAAAGGCTGAGCCCCGCGGGGCTGCTCAGCAGCCCTGACATCACCCATGCCGCACCCTTCTGCGCTGGCAATCTTCGGGATGTAAGACCCGCCCATCCTGGCCACGGACCTGCAATATTTCAAGCGCCTGCCCCGTCACGCCGTCCACCAGTTCCGAACGCGCTTCTCCCGCCTC > NC_002947/591558‑591854 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |