Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 646,275 T→A 11.2% intergenic (‑178/‑106) PP_0556 ← / → acoR acetoin catabolism protein/acetoin catabolism regulatory protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947646,2750TA11.2% 148.7 / 8.7 54intergenic (‑178/‑106)PP_0556/acoRacetoin catabolism protein/acetoin catabolism regulatory protein
Reads supporting (aligned to +/- strand):  ref base T (23/24);  new base A (3/3);  total (26/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.41e-01

AGGAGCAAGCGGGGTGCCAGCTTTCTTCGCGATGGCTGAAGTGGCCGGGCTAGAGGGCTCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACGAGAACCGGAGGGCCTGAATGCTTGCCGCGAACTCCCGAGCCCATGTGGACTGCGTCAGCAG  >  NC_002947/646128‑646424
                                                                                                                                                   |                                                                                                                                                     
aGGAGCAAGCGGGGTGCCAGCTTTCTTCGCGATGGCTGAAGTGGCCGGGCTAGAGGGCTCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGc                                                                                                                                                     <  1:80323/150‑1 (MQ=255)
       aGCGGGGTGCCAGCTTTCTTCGCGATGGCTGAAGTGGCCGGGCTAGAGGGCTCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTCGCCGAGACACTGAGACGCTGCGTCTCaa                                                                                                                                              <  2:327012/150‑1 (MQ=255)
             gTGCCAGCTTTCTTCGCGATGGCTGAAGTGGCCGGGCTAGAGGGCTCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAagccagc                                                                                                                                        <  1:917898/150‑1 (MQ=255)
                ccAGCTTTCTTCGCGATGGCTGAAGTGGCCGGGCTAGAGGGCTCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCgg                                                                                                                                     >  1:119598/1‑150 (MQ=255)
                                    tGAAGTGGCCGGGCTAGAGGGCTCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAag                                                                                                                                             <  1:757613/122‑1 (MQ=255)
                                    tGAAGTGGCCGGGCTAGAGGGCTCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAag                                                                                                                                             >  2:757613/1‑122 (MQ=255)
                                          ggCCGGGCTAGAGGGCTCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGACTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCAt                                                                                                           <  2:1135743/150‑1 (MQ=255)
                                           gCCGGGCTAGAGGGCTCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATg                                                                                                          >  1:357092/1‑150 (MQ=255)
                                             cGGGCTAGAGGGCTCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGcc                                                                                                        >  1:128744/1‑150 (MQ=255)
                                              gggCTAGAGGGCTCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCAGCGTCTCagt                                                                                                                                             <  2:504241/112‑3 (MQ=255)
                                              gggCTAGAGGGCTCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCAGCGTCTCagt                                                                                                                                             >  1:504241/1‑110 (MQ=255)
                                                   agagGGCGCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAgg                                                                                                  <  2:660078/150‑1 (MQ=255)
                                                    gagGGCTCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGgc                                                                                                 >  1:827636/1‑150 (MQ=255)
                                                      gggCTCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTACCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGgcgc                                                                                               <  2:1508020/150‑1 (MQ=255)
                                                          tctGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGc                                                                                                                       >  1:898031/1‑122 (MQ=255)
                                                          tctGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGc                                                                                                                       <  2:898031/122‑1 (MQ=255)
                                                          tctGGTCGTGTTCGGGCAGGATTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTaa                                                                                           >  2:558500/1‑150 (MQ=255)
                                                              gTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTa                                                                                            >  2:72162/1‑145 (MQ=255)
                                                              gTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTa                                                                                            <  1:72162/145‑1 (MQ=255)
                                                                cGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGAAGCCCAGACCTATCGTCTTCCCTTCTTTGCCGAGCCACCTAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCg                                                                                     <  1:366690/150‑1 (MQ=255)
                                                                      cGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAAc                                                                               <  2:827636/150‑1 (MQ=255)
                                                                             gctttCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCTAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATaaaa                                                                        >  2:1603073/3‑150 (MQ=255)
                                                                                       gTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATaaa                                                                         >  2:98932/1‑139 (MQ=255)
                                                                                       gTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCATGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATaaa                                                                         <  1:98932/139‑1 (MQ=255)
                                                                                          gggTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACGAGaa                                                           >  2:924299/1‑150 (MQ=255)
                                                                                                cGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACGAGAACCGGAg                                                     <  1:1120822/150‑1 (MQ=255)
                                                                                                 gagaCGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCt                                                                                              >  2:159554/1‑108 (MQ=255)
                                                                                                 gagaCGCCGGGTGCCAGCGCCGGGCCTGCGTTGCAGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCt                                                                                              <  1:159554/108‑1 (MQ=255)
                                                                                                  agaCGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACGAGAACCGGAggg                                                   <  1:924299/150‑1 (MQ=255)
                                                                                                       ccGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACGAGAACCGGAGGGCCTGa                                              <  1:1615775/150‑1 (MQ=255)
                                                                                                            tGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACa                                                                     >  2:531866/1‑122 (MQ=255)
                                                                                                            tGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACa                                                                     <  1:531866/122‑1 (MQ=255)
                                                                                                              ccAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATa                                                                           <  2:288972/114‑1 (MQ=255)
                                                                                                              ccAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATa                                                                           >  1:288972/1‑114 (MQ=255)
                                                                                                              ccAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACGAGAACCGGAGGGCCTGAATGCTTg                                       >  2:1646198/1‑150 (MQ=255)
                                                                                                                aGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGgc                                                                                                 >  2:1409762/1‑90 (MQ=255)
                                                                                                                aGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGgc                                                                                                 <  1:1409762/90‑1 (MQ=255)
                                                                                                                  cgcCGGGCCTGCGTTGCCGAGACACTGAGACGCTGAGGCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGGTAAATTGTCGGACAACGATAAAAACAGTAACGAGAACCGGAGGGCCTGAACGCTTGCcgc                                   <  2:557704/150‑1 (MQ=255)
                                                                                                                         ccTGCGTTGCCTAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACGAGAACCGGAGGGCCTGAATGCTTGCCGCGAACTcc                            <  1:1603073/150‑1 (MQ=255)
                                                                                                                          cTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACGAGAACCGGAGGGCCTGAATGCTTGCCGCGAACTccc                           >  2:1347599/1‑150 (MQ=255)
                                                                                                                            gCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACGAGAACCGGAGGGCCTGAATGCTTGCCGCGAACTCCCGa                         <  1:558500/150‑1 (MQ=255)
                                                                                                                               ttGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGt                                                                                                                                    >  2:716770/1‑40 (MQ=255)
                                                                                                                               ttGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGt                                                                                                                                    <  1:716770/40‑1 (MQ=255)
                                                                                                                                  ccGAGACACTGAGACGCGGCGTCTCAAGCCAGCCGGTAATCAGACGGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACGCGAACCGGAGGGCCTGAATGCTCGCCGCGAACTCCCGAGCCCAt                   <  2:357092/150‑1 (MQ=255)
                                                                                                                                   cgggaCACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACGAGAACCGGAGGGCCTGAATGCTTGCCGCGAACTCCCGAGCCCAtg                  >  1:1642723/4‑150 (MQ=255)
                                                                                                                                    gagaCACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACGAGAACCGGAGGGCCTGAATGCTTGCCGCGAACTCCCGAGCCCAtgt                 >  1:422818/1‑150 (MQ=255)
                                                                                                                                        gcttGAGACGCAGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACGAGAACCGGAGGGCCTGAATGCTTGCcgc                                   >  2:506553/4‑128 (MQ=255)
                                                                                                                                        gcttGAGACGCAGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACGAGAACCGGAGGGCCTGAATGCTTGCcgc                                   <  1:506553/125‑1 (MQ=255)
                                                                                                                                         cttGAGACGCAGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATg                                                                                         <  2:519869/71‑1 (MQ=255)
                                                                                                                                         cttGAGACGCAGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATg                                                                                         >  1:519869/3‑73 (MQ=255)
                                                                                                                                           tGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACGAGAACCGGAGGGCCTGAATGCTTGCCGCGAACTCCCGAGCCCATGTGGACTGc          >  2:270694/1‑150 (MQ=255)
                                                                                                                                            gagaCGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGt                                                                   >  2:859974/1‑92 (MQ=255)
                                                                                                                                            gagaCGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGt                                                                   <  1:859974/92‑1 (MQ=255)
                                                                                                                                               aCGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACGAGAACCGGAGGGCCTGAATGCTTGCCGCGAACTCCCGAGCCCATGTGGACTGCGTca      >  1:307457/1‑150 (MQ=255)
                                                                                                                                                   tGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACGAGAACCGGAGGGCCTGAATGCTTGCCGCGAACTCCCGAGCCCATGTGGACTGCGTcagcag  >  2:802223/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
AGGAGCAAGCGGGGTGCCAGCTTTCTTCGCGATGGCTGAAGTGGCCGGGCTAGAGGGCTCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACGAGAACCGGAGGGCCTGAATGCTTGCCGCGAACTCCCGAGCCCATGTGGACTGCGTCAGCAG  >  NC_002947/646128‑646424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑