Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	648,294	G→T	9.1%	intergenic (+60/+31)	acoR → / ← accC	acetoin catabolism regulatory protein/acetyl‑CoA carboxylase biotin carboxylase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	648,294	0	G	T	9.1%	222.4 / 8.0	74	intergenic (+60/+31)	acoR/accC	acetoin catabolism regulatory protein/acetyl‑CoA carboxylase biotin carboxylase subunit
Reads supporting (aligned to +/- strand):  ref base G (31/36);  new base T (5/2);  total (36/38)
Fisher's exact test for biased strand distribution p-value = 2.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01

TGCGGCACGGGCTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGTTGGTCTTGATGCCGTCGACGA  >  NC_002947/648147‑648443                                                                                                                                                    |                                                                                                                                                      tGCGGCACGGGCTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGc                                                                                                                                                     >  2:890084/1‑150 (MQ=255)   cGGCACGGGCTTTGCGCATTTCCCGGGCGACGCTTTCTCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCg                                                                                                                                                   <  1:329989/150‑1 (MQ=255)     gCACGGGCTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCggg                                                                                                                                                 >  2:1560404/1‑150 (MQ=255)       aCGGGCTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGt                                                                                                                                               <  1:1583585/150‑1 (MQ=255)         gggCTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCg                                                                                                                                                   <  2:1487116/144‑1 (MQ=255)         gggCTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCg                                                                                                                                                   >  1:1487116/1‑144 (MQ=255)         gggCTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTc                                                                                                                                             >  2:1124223/1‑150 (MQ=255)            cTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTgg                                                                                                                                          <  2:1517727/150‑1 (MQ=255)                gggCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACa                                                                                                                                      >  2:260493/1‑150 (MQ=255)                 ggCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCCCTGTGCCGGGGttt                                                                                                                                             >  2:102065/1‑141 (MQ=255)                 ggCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCCCTGTGCCGGGGttt                                                                                                                                             <  1:102065/142‑2 (MQ=255)                 ggCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACAt                                                                                                                                     <  1:260493/150‑1 (MQ=255)                        cgcgGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTa                                                                                                                              <  1:166591/150‑1 (MQ=255)                                  gCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCCCTGTGCCGGGGttt                                                                                                                                             <  2:1325473/125‑2 (MQ=255)                                  gCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCCCTGTGCCGGGGttt                                                                                                                                             >  1:1325473/1‑124 (MQ=255)                                        tCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGt                                                                                                              <  1:954137/150‑1 (MQ=255)                                        tCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCAGGCACAGTGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGt                                                                                                              >  2:1123438/1‑150 (MQ=255)                                                  cATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGttt                                                                                                    <  1:402400/150‑1 (MQ=255)                                                   aTGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGCTTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTATGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTtg                                                                                                   >  1:429075/1‑150 (MQ=255)                                                     gAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTtgtg                                                                                                 <  2:621284/150‑1 (MQ=255)                                                     gAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTtgtg                                                                                                 <  1:1124223/150‑1 (MQ=255)                                                            gCATCGACGTGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAgg                                                                                          <  1:1484263/150‑1 (MQ=255)                                                            gCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAgg                                                                                          <  2:346452/150‑1 (MQ=255)                                                                 gACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTa                                                                                                                              <  2:141646/109‑1 (MQ=255)                                                                 gACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTa                                                                                                                              >  1:141646/1‑109 (MQ=255)                                                                  aCATGCCGCGGAGAAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGa                                                                                    <  1:64908/150‑1 (MQ=255)                                                                   cATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGaa                                                                                   <  1:536742/150‑1 (MQ=255)                                                                        cgcgGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGtt                                                                               >  1:328147/1‑149 (MQ=255)                                                                        cgcgGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGtt                                                                               <  2:328147/149‑1 (MQ=255)                                                                          cgGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGAc                                                                            <  1:1560404/150‑1 (MQ=255)                                                                            gATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGAcgc                                                                          >  1:859770/1‑150 (MQ=255)                                                                              tGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTgg                                                                                                               >  2:1493072/1‑111 (MQ=255)                                                                              tGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTgg                                                                                                               <  1:1493072/111‑1 (MQ=255)                                                                               gAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGAcgccgc                                                                       >  2:664889/1‑150 (MQ=255)                                                                                  ggggTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCtt                                                                    >  2:796980/1‑150 (MQ=255)                                                                                   gggTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCttt                                                                   <  1:159248/150‑1 (MQ=255)                                                                                        gagCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGAcgccgc                                                                       <  1:1538066/141‑1 (MQ=255)                                                                                        gagCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGAcgccgc                                                                       >  2:1538066/1‑141 (MQ=255)                                                                                        gagCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCACTAAGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGa                                                              >  1:499374/1‑150 (MQ=255)                                                                                               ggggCCGCGTCGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTtc                                                       >  1:1198716/1‑150 (MQ=255)                                                                                                   ccGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTtcatca                                                   >  1:595539/1‑150 (MQ=255)                                                                                                       gACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCa                                               <  2:859770/150‑1 (MQ=255)                                                                                                             gCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGTACCAGGt                                         <  2:681907/150‑1 (MQ=255)                                                                                                                ccAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGtt                                                                                                     <  2:471654/87‑1 (MQ=255)                                                                                                                ccAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGtt                                                                                                     >  1:471654/1‑87 (MQ=255)                                                                                                                ccAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGc                                                           <  1:382847/129‑1 (MQ=255)                                                                                                                ccAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGc                                                           >  2:382847/1‑129 (MQ=255)                                                                                                                ccAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTcgc                                      >  2:790419/1‑150 (MQ=255)                                                                                                                ccAGATTCTAGCAAGCAATAAAAGACCCCGGCACAGGACCGGGGGTCTGGCACATCACACTACACGATGGCTCCGGGTTGGGCAGCTTGTGCTGCAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGGCTTCCTCACGCGCTAGGTcgc                                      >  2:1595400/1‑150 (MQ=255)                                                                                                                  ggATTCTGGGAGGCAATAGAAAAGCCCGGCACGGGTGCGGGTTTCTGGTACAGCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGGATGGTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCgg                                    <  2:1599118/149‑1 (MQ=255)                                                                                                                      tCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCa                                >  2:1027590/1‑150 (MQ=255)                                                                                                                           caggcaATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCggg                           >  2:522591/1‑150 (MQ=255)                                                                                                                           caggcaATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCggg                           <  1:522591/150‑1 (MQ=255)                                                                                                                              gcaATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTc                                                                                                                   >  2:640239/1‑59 (MQ=255)                                                                                                                              gcaATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTc                                                                                                                   <  1:640239/59‑1 (MQ=255)                                                                                                                                 aTAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGTTg                     <  2:595539/150‑1 (MQ=255)                                                                                                                                 aTAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGTTg                     <  1:1027590/150‑1 (MQ=255)                                                                                                                                 aTAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGTTg                     <  1:664889/150‑1 (MQ=255)                                                                                                                                  tAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGAcgccgc                                                                       <  2:684550/99‑1 (MQ=255)                                                                                                                                  tAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGAcgccgc                                                                       >  1:684550/1‑99 (MQ=255)                                                                                                                                    cagaaCCCCGGCCCTGTGCCGGGGTTTTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGTTGGTc                  >  2:1281933/4‑150 (MQ=255)                                                                                                                                    cagaaCCCCGGCCCTGTGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGTTGGTc                  >  2:1451581/4‑150 (MQ=255)                                                                                                                                        tccccGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATTTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGTTGGTCTTGa              <  2:105254/149‑1 (MQ=255)                                                                                                                                        aCCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGTTGGTCTTGa              <  1:890084/150‑1 (MQ=255)                                                                                                                                         ccccGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGTTGGTCTTGAt             >  1:351909/1‑150 (MQ=255)                                                                                                                                           ccGGCACAGGGCCGGGGTTCTGGTACATCACACTaca                                                                                                                            >  2:972656/1‑37 (MQ=255)                                                                                                                                           ccGGCACAGGGCCGGGGTTCTGGTACATCACACTaca                                                                                                                            <  1:972656/37‑1 (MQ=255)                                                                                                                                           ccGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTcgcg                                     >  2:1271062/1‑124 (MQ=255)                                                                                                                                           ccGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTcgcg                                     <  1:1271062/124‑1 (MQ=255)                                                                                                                                           ccGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGTTGGTCTTGATGc           >  1:1260221/1‑150 (MQ=255)                                                                                                                                            cGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAg                                                                                           <  2:773187/69‑1 (MQ=255)                                                                                                                                            cGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAg                                                                                           >  1:773187/1‑69 (MQ=255)                                                                                                                                                acaGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCGGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGTTGGTCTTGATGCCGTcg      >  2:1176670/1‑150 (MQ=255)                                                                                                                                                  cgggCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGTTGGTCTTGa              <  1:1486492/139‑1 (MQ=255)                                                                                                                                                  aGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGTTGGTCTTGa              >  2:1486492/1‑140 (MQ=255)                                                                                                                                                    ggCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGAc                                                                            <  2:325829/76‑1 (MQ=255)                                                                                                                                                    ggCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGAc                                                                            >  1:325829/1‑76 (MQ=255)                                                                                                                                                    ggCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGTTGGTCTTGATGCCGTcgacga  >  2:1009721/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                                      TGCGGCACGGGCTTTGGGCATTTCGCGGGCGACGCTTTATCGGCGAGTGCATGAGCACCGCATCGACATGCCGCGGATGAAGGGGTAGAGCCCTGGGGCCGCGACGCGGCCCCAGATTCTGGCAGGCAATAAAAAACCCCGGCACAGGGCCGGGGTTCTGGTACATCACACTACACGATCACTCCTGGTTGGCCAGTTTGTGCTCGAGGTAGTGAATGTTGACGCCGCCTTTGCAGAAGCCTTCATCACGCACCAGGTCGCGGTGCAGCGGGATGTTGGTCTTGATGCCGTCGACGA  >  NC_002947/648147‑648443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑