| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 838,760 | T→A | 6.2% | *314L (TAA→TTA) | prs ← | ribose‑phosphate pyrophosphokinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 838,760 | 0 | T | A | 6.2% | 199.0 / 4.7 | 64 | *314L (TAA→TTA) | prs | ribose‑phosphate pyrophosphokinase |
| Reads supporting (aligned to +/- strand): ref base T (30/30); new base A (2/2); total (32/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.09e-01 | |||||||||||
GCTTGGGCGTTCAGAGTGAATTCAGTCATTTTGTTTCTCCAAAGTAGCCCCCCGAGGGCGTTTGCGACCAGCGCCAACGGGGATGGCAAAAAAGCCCCGCCCCAACACAGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGCTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGTCACAGGCTTGAGCAGCGGCAGACAGCGGAACGGTGTTGGTCACCACCAGCTCGTCCA > NC_002947/838612‑838907 | gcTTGGGCGTTCAGAGTGAATTCAGTCATTTTGTTTCTCCAAAGTAGCCCCCCGAGGGCGTTTGCGACCAGCGCCAACGGGGATGGCAAAAAAGCCCCGCCCCAACACAGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTa < 1:280607/150‑1 (MQ=255) aaTCCAGTCATTTTGTTTCTCCAAAGTAGCCCCCCGAGGGCGTTTGCGACCAGCGCCAACGGGGATGGCAAAAAAGCCCCGCCCCAACACAGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGAt < 1:1128956/150‑1 (MQ=255) aTTTTGTTTCTCCAAAGTAGCCCCCCGAGGGCGTTTGCGACCAGCGCCAACGGGGATGGCAAAAAAGCCCCGCCCCAACACAGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCCTCGCGGAACATCGCGCTCATCGActcttc > 2:582861/1‑150 (MQ=255) ggttCTCCAAAGTAGCCCCCCGAGGGCGTTTGCGACCAGCGCCAACGGGGATGGCAAAAAAGCCCCGCCCCAACATCCGTTGGGGCGGGGCGCTTCACATCAACACGTCTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGc < 2:583764/148‑1 (MQ=255) tcCAAAGTAGCCCCCCGAGGGCGTTTGCGACCAGCGCCAACGGGGATGGCAAAAAAGCCCCGCCCCAACACAGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGa > 2:818716/1‑130 (MQ=255) tcCAAAGTAGCCCCCCGAGGGCGTTTGCGACCAGCGCCAACGGGGATGGCAAAAAAGCCCCGCCCCAACACAGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGa < 1:818716/130‑1 (MQ=255) tcCAAAGTAGCCCCCCGAGGGCGTTTGCGACCAGCGCCAACGGGGATGGCAAAAAAGCCCCGCCCCAACACAGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATg > 2:1645405/1‑150 (MQ=255) cccccGAGGGCGTTTGCGACCAGCGCCAACGGGGATGGCAAAAAAGCCCCGCCCCAACACAGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGa > 1:221801/1‑137 (MQ=255) cccccGAGGGCGTTTGCGACCAGCGCCAACGGGGATGGCAAAAAAGCCCCGCCCCAACACAGGTTGGGGCGGGGCGCTTCACAGCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGa < 2:221801/137‑1 (MQ=255) ccccGAGGGCGTTTGCGACCAGCGCCAACGGGGATGGCAAAAAAGCCCCGCCCCAACACAGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGCt > 1:1497510/1‑150 (MQ=255) aGGGCGTTTGCGACCAGCGCCAACGGGGATGGCAAAAAAGCCCCGCCCCAACACAGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGCTTCAGc < 2:547253/150‑1 (MQ=255) gCGTTTGCGACCAGCGCCAACGGGGATGGCAAAAAAGCCCCGCCCCAACACAGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGCTTCAGCGAc < 1:1153095/150‑1 (MQ=255) gTTTGCGACCAGCGCCAACGGGGATGGCAAAAAAGCCCCGCCCCAACACAGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGCTGCAGCTGACCGCTTCAGCGACaa > 1:1318067/1‑150 (MQ=255) tttGCGACCAGCGCCAACGGGGATGGCAAAAAAGCCCGGCCCCAACACAGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGCTTCATCGACAAc > 2:739526/1‑150 (MQ=255) aaCGGGGATGGCAAAAAAGCCCCGCCCCAACACAGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGCTTCAGCGACAACCGGTGCGATATCCAg > 1:891527/1‑150 (MQ=255) gATGGCAAAAAAGCCCCGCCCCAACACAGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGCTTCAGCGACAACCGGTGCGATATCCAGCTGTCg < 1:1645405/150‑1 (MQ=255) aTGGCAAAAAAGCCCCGCCCCAACACAGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGCTTCAGCGACAACCGGTGCGATATCCAGCTGGCgg > 1:240398/1‑150 (MQ=255) tGGCAAAAAAGCCCCGCCCCAACACAGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGCTTCAGCGACAACCGGTGCGa < 1:1122538/135‑1 (MQ=255) aggCAAAAAAGCCCCGCCCCAACACAGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGCTTCAGCGACAACCGGTGCGa > 2:1122538/2‑135 (MQ=255) aaaaaaGCCCCGCCCCAACACAGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGt > 2:772956/1‑92 (MQ=255) aaaaaaGCCCCGCCCCAACACAGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGt < 1:772956/92‑1 (MQ=255) aaaaaGCCCCGCCCCAACACAGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGCTTCAGCGACAACCGGTGc > 1:736196/1‑128 (MQ=255) aaaaaGCCCCGCCCCAACACAGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGCTTCAGCGACAACCGGTGc < 2:736196/128‑1 (MQ=255) aaaaaGCCCCGCCCCAACACAGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGCCTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACgg < 1:582861/150‑1 (MQ=255) aaaaGCCCCGCCCCAACACAGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGaa > 2:1083310/1‑67 (MQ=255) aaaaGCCCCGCCCCAACACAGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGaa < 1:1083310/67‑1 (MQ=255) aaaaGCCCCGCCCCAACACAGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGCTTCAGCGACAACCGGTGc < 1:619135/127‑1 (MQ=255) aaaaGCCCCGCCCCAACACAGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGCTTCAGCGACAACCGGTGc > 2:619135/1‑127 (MQ=255) ccccgccccAACCTGTGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGCTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGa < 2:1445148/139‑1 (MQ=255) ccccgccccAACCTGTGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGCTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGa > 1:1445148/1‑139 (MQ=255) cccgccccAACACAGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGCTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGTCACAgg > 2:1554884/1‑150 (MQ=255) cccgccccAACACAGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGCTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGTCACAgg > 2:1356989/1‑150 (MQ=255) ccgccccAACACAGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTAAGCGGGACac < 1:621981/63‑2 (MQ=255) ccgccccAACACAGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTAAGCGGGACac > 2:621981/1‑62 (MQ=255) ccAACACAGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGCTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGTCACAGGCTTGag < 2:1308166/150‑1 (MQ=255) cAACACAGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGCTTCAGCGACAACCGGTGCGATATCCAGCTGGCg < 1:577578/129‑1 (MQ=255) cAACACAGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGCTTCAGCGACAACCGGTGCGATATCCAGCTGGCg > 2:577578/1‑129 (MQ=255) cAACACAGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGCTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGTCACAGGCTTGagc > 2:72796/1‑150 (MQ=255) acaGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGCTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGTCACAGGCTTGAGCAGCg > 1:20265/1‑150 (MQ=255) ggTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGCTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGTCACAGGCTTGAGCAGCGGCa < 2:240398/150‑1 (MQ=255) gTTGGGGCGGGGCGCTTCACATCAACACGTGTTCCGCTAAGCGGGACac > 1:646528/1‑48 (MQ=21) gTTGGGGCGGGGCGCTTCACATCAACACGTGTTCCGCTAAGCGGGACac < 2:646528/49‑2 (MQ=21) gTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGCTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGt > 2:1283776/1‑129 (MQ=255) gTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGCTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGt < 1:1283776/129‑1 (MQ=255) ttGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGCACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGCTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGTCACAGGCTTGAGCAGCGGCAGa < 1:589501/150‑1 (MQ=255) gcggggcgCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGCTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGTCACAGGCTTGAGCAGCGGCAGACAGCg < 2:20265/150‑1 (MQ=255) gcggggcgCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGCTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGTCACAGGCTTGAGCAGCGGCAGACAGCg > 2:1224502/1‑150 (MQ=255) ttCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGCTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGt < 1:595396/114‑1 (MQ=255) ttCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGCTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGt > 2:595396/1‑114 (MQ=255) ttCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGCTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGTCACAGGCTTGAGCAGCGGCAGACAGCGGAACGgtgt < 1:1421402/150‑1 (MQ=255) aTCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGCTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGTCACAGGCt > 2:1520887/1‑117 (MQ=255) aTCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGCTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGTCACAGGCt < 1:1520887/117‑1 (MQ=255) cacGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGCTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGTCACAGGCTTGAGCAGCGGCAGACAGCGGAAc > 2:118966/1‑135 (MQ=255) cacGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGCTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGTCACAGGCTTGAGCAGCGGCAGACAGCGGAAc < 1:118966/135‑1 (MQ=255) cccGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGCTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGTCACAGGCTTGAGCAGCGGCAGACAGCGGAACGGTGTTGGTCACCACCAGCTCg > 2:1246477/1‑150 (MQ=255) cccGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGCTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGTCACAGGCTTGAGCAGCGGCAGACAGCGGAACGGTGTTGGTCACCACCAGCTCg > 2:434673/1‑150 (MQ=255) ccGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGCTTCAGCGACAACCGGTGCGATATCCAGc > 1:59874/1‑83 (MQ=255) ccGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGCTTCAGCGACAACCGGTGCGATATCCAGc < 2:59874/83‑1 (MQ=255) ccGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGCTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGTCACAGGCTTGAGCAGCGGCAGACAGc < 1:280277/122‑1 (MQ=255) ccGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGCTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGTCACAGGCTTGAGCAGCGGCAGACAGc > 2:280277/1‑122 (MQ=255) ccGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGCTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGTCACAGGCTTGAGCAGCGGCAGACAGCGGAACGGTGTTGGTCACCACCAGCTCGt < 1:1246477/150‑1 (MQ=255) gCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGCTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATa < 2:1099806/90‑1 (MQ=255) gCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGCTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATa > 1:1099806/1‑90 (MQ=255) cTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGCTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGTCACAGGCTTGAGCAGCGGCAGACAGCGGAACGGTGTTGGTCACCACCAGCTCGTCCa < 2:1318067/150‑1 (MQ=255) | GCTTGGGCGTTCAGAGTGAATTCAGTCATTTTGTTTCTCCAAAGTAGCCCCCCGAGGGCGTTTGCGACCAGCGCCAACGGGGATGGCAAAAAAGCCCCGCCCCAACACAGGTTGGGGCGGGGCGCTTCACATCAACACGTGTCCCGCTTAGCGGAACATCGCGCTGATCGATTCTTCGTTGCTGATGCGGCGTACCGCTTCAGCGACAACCGGTGCGATATCCAGCTGGCGGATACGGTCACAGGCTTGAGCAGCGGCAGACAGCGGAACGGTGTTGGTCACCACCAGCTCGTCCA > NC_002947/838612‑838907 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |