Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 104,876 G→C 12.1% P345P (CCG→CCC)  PP_0099 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947104,8760GC12.1% 140.6 / 9.5 48P345P (CCG→CCC) PP_0099hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (20/22);  new base C (3/3);  total (23/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCTGTATGACAGCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGTTCCCGCCT  >  NC_002947/104728‑105020
                                                                                                                                                    |                                                                                                                                                
aGCTGTATGACAGCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCgg                                                                                                                                                 <  2:1342031/150‑1 (MQ=255)
  cTGTATGACAGCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGt                                                                                                                                               <  1:300263/150‑1 (MQ=255)
          cAGCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGAt                                                                                                                                       <  2:1567372/150‑1 (MQ=255)
           aGCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAACCGGACGACAACCGAAGTGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGTTGGGCAGATg                                                                                                                                      >  1:529848/1‑150 (MQ=255)
            gCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAACGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCCGGTGGGCaaaacg                                                                                                                                     <  1:344930/150‑6 (MQ=255)
                     gCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCt                                                                                                                            <  2:849121/150‑1 (MQ=255)
                          cgcATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAAAGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACAt                                                                                                                       <  2:140112/150‑1 (MQ=255)
                                 gtggtgAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGgc                                                                                                                                    <  2:994874/130‑1 (MQ=255)
                                 gtggtgAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGgc                                                                                                                                    >  1:994874/1‑130 (MQ=255)
                                    gtgAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAaccccacc                                                                                                             >  2:365737/1‑150 (MQ=255)
                                     tgAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAaccccaccc                                                                                                            >  1:853125/1‑150 (MQ=255)
                                      gAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAaccccacccc                                                                                                           >  1:87667/1‑150 (MQ=255)
                                             tATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGTCGCTGTTCTGACATGAACCCCACCCCCCACAgg                                                                                                    <  1:949318/150‑1 (MQ=255)
                                                         tGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTgcgc                                                                                           >  1:281705/1‑147 (MQ=255)
                                                         tGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTgcgc                                                                                           <  2:281705/147‑1 (MQ=255)
                                                              gcgcAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTca                                                                                   <  2:869759/150‑1 (MQ=255)
                                                                gcAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTg                                                                                       <  2:599577/144‑1 (MQ=255)
                                                                gcAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTg                                                                                       >  1:599577/1‑144 (MQ=255)
                                                                          gCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAg                                                                                                                                         >  1:620118/1‑84 (MQ=255)
                                                                          gCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAg                                                                                                                                         <  2:620118/84‑1 (MQ=255)
                                                                           ccAAGGCAATGAACCGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCCGCGTTTTGCCCACCCGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCCAATTGCTACCAACGCAACcaat                                                                      >  2:89078/1‑150 (MQ=255)
                                                                           ccAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACtaat                                                                      <  2:853125/150‑1 (MQ=255)
                                                                            cAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACtaata                                                                     >  2:827389/1‑150 (MQ=255)
                                                                            cAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACtaata                                                                     >  2:1151568/1‑150 (MQ=255)
                                                                             aaGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACtaataa                                                                    >  2:1014341/1‑150 (MQ=255)
                                                                                 cAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGTTTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATaacgca                                                                <  1:1291600/150‑4 (MQ=255)
                                                                                           gCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTgc                                                      <  1:1014341/150‑1 (MQ=255)
                                                                                             tGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTgcgc                                                    <  2:1615629/150‑1 (MQ=255)
                                                                                                  ttCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATa                                               <  1:164946/150‑1 (MQ=255)
                                                                                                       aGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCaa                                                                           <  2:1573201/117‑1 (MQ=255)
                                                                                                       aGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCaa                                                                           >  1:1573201/1‑117 (MQ=255)
                                                                                                                       gAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGtttt                          >  2:947696/1‑150 (MQ=255)
                                                                                                                             gATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCt                    >  1:540848/1‑150 (MQ=255)
                                                                                                                              aTTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCta                   <  2:540848/150‑1 (MQ=255)
                                                                                                                                        gTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCaa                                                                           <  2:1383494/84‑1 (MQ=255)
                                                                                                                                        gTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCaa                                                                           >  1:1383494/1‑84 (MQ=255)
                                                                                                                                        catctGCCCACCCGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATc              <  1:1378989/141‑1 (MQ=255)
                                                                                                                                        catctGCCCACCCGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATc              >  2:1378989/5‑145 (MQ=255)
                                                                                                                                         ttttGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGTTc        >  2:1538677/1‑150 (MQ=255)
                                                                                                                                         atctGCCCACCCGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACaa                                                                                >  2:1562444/4‑78 (MQ=255)
                                                                                                                                         atctGCCCACCCGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACaa                                                                                <  1:1562444/75‑1 (MQ=255)
                                                                                                                                             gCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCTCCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGTTCCCGc    <  1:89078/150‑1 (MQ=255)
                                                                                                                                               ccACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCaa                                                             <  1:865106/91‑1 (MQ=255)
                                                                                                                                               ccACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCaa                                                             >  2:865106/1‑91 (MQ=255)
                                                                                                                                               ccACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTg                      >  2:792489/1‑130 (MQ=255)
                                                                                                                                               ccACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTg                      <  1:792489/130‑1 (MQ=255)
                                                                                                                                               ccACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTTTTATTCAGTTCCTGCCt  >  2:755015/1‑150 (MQ=255)
                                                                                                                                                cACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAAc                                   >  2:1380312/1‑116 (MQ=255)
                                                                                                                                                cACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAAc                                   <  1:1380312/116‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
AGCTGTATGACAGCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGTTTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGTTCCCGCCT  >  NC_002947/104728‑105020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑