Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 883,809 T→G 7.1% intergenic (+44/‑284) PP_0766 → / → PP_0767 hypothetical protein/LuxR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947883,8090TG 7.1% 161.6 / 3.4 57intergenic (+44/‑284)PP_0766/PP_0767hypothetical protein/LuxR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (23/29);  new base G (2/2);  total (26/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

CTTGGGATGAAGAACGAAGAGAAACGTGCCTACGACTTCGGCTTCAGTGCCAGCAAGGCCGATTTCCAGCCCGCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGTTCCGCGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCAAGGCAATCAACCTCCTGAATACCTCTCCAAACCCCCGCCCCAG  >  NC_002947/883664‑883952
                                                                                                                                                 |                                                                                                                                               
cTTGGGATGAAGAACGAAGAGAAACGTGCCTACGACTTCGGCTTCAGTGCCAGCAAGGCCGATTTCCAGCCCGCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGcc                                                                                                                                             >  2:1614231/1‑150 (MQ=255)
   gggATGAAGAACGAAGAGAAACGTGCCTACGACTTCGGCTTCAGTGCCAGCAAGGCCGATTTCCAGCCCGCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCggg                                                                                                                                          <  1:69814/150‑1 (MQ=255)
     gATGAAGAACGAAGAGAAACGTGCCTACGACTTCGGCTTCAGTGCCAGCAAGGCCGATTTCCAGCCCGCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGc                                                                                                                                        >  2:1219965/1‑150 (MQ=255)
      aTGAAGAACGAAGAGAAACGTGCCTACGACTTCGGCTTCAGTGCCAGCAAGGCCGATTTCCAGCCCGCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCg                                                                                                                                       >  1:560159/1‑150 (MQ=255)
         aagaaCGAAGAGAAACGTGCCTACGACTTCGGCTTCAGTGCCAGCAAGGCCGATTTCCAGCCCGCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGttt                                                                                                                                    <  2:1127041/150‑1 (MQ=255)
               gAAGAGAAACGTGCCTACGACTTCGGCTTCAGTGCCAGCAAGGCCGATTTCCAGCCCGCAGCGCTGCGTCAGTCGGGTATTTGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTAttt                                                                                                                              >  2:307294/1‑150 (MQ=255)
                 agagAAACGTGCCTACGACTTCGGCTTCAGTGCCAGCAAGGCCGATTTCCAGCCCGCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTcc                                                                                                                            <  2:151512/150‑1 (MQ=255)
                               aCGACTTCGGCTTCAGTGCCAGCAAGGCCGATTTCCAGCCCGCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGc                                                                                                                                              <  2:1369686/118‑1 (MQ=255)
                               aCGACTTCGGCTTCAGTGCCAGCAAGGCCGATTTCCAGCCCGCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGc                                                                                                                                              >  1:1369686/1‑118 (MQ=255)
                               aCGACTTCGGCTTCAGTGCCAGCAAGGCCGATTTCCAGCCCGCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCtt                                                                                                              >  1:590418/1‑150 (MQ=255)
                                  aCTTCGGCTTCAGTGCCAGCAAGGCCGATTTCCAGCCCGCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTcgc                                                                                                           <  2:1596108/150‑1 (MQ=255)
                                    ttCGGCTTCAGTGCCAGCAAGGCCGATTTCCAGCCCGCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCt                                                                                                                           <  2:798035/132‑1 (MQ=255)
                                    ttCGGCTTCAGTGCCAGCAAGGCCGATTTCCAGCCCGCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCt                                                                                                                           >  1:798035/1‑132 (MQ=255)
                                    ttCGGCTTCAGTGCCAGCAAGGCCGATTTCCAGCCCGCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGc                                                                                                                   >  2:1151841/1‑140 (MQ=255)
                                    ttCGGCTTCAGTGCCAGCAAGGCCGATTTCCAGCCCGCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGc                                                                                                                   <  1:1151841/140‑1 (MQ=255)
                                    ctCGGCTTCAGTGCCAGCAAGGCCGATTTCCAGCCCGCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCgg                                                                                                         <  1:433454/149‑1 (MQ=255)
                                       ggCTTCAGTGCCAGCAAGGCCGATTTCCAGCCCGCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCCCGCCGGGGCGTTTCTATTTCCTGACCCGCCCTCTTCCCgggag                                                                                                      >  2:433454/1‑148 (MQ=255)
                                          ttCAGTGCCAGCAAGGCCGATTTCCAGCCCGCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAAc                                                                                                   <  2:566497/150‑1 (MQ=255)
                                               tGCCAGCAAGGCCGATTTCCAGCCCGCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGtttt                                                                                                                                   >  1:499672/1‑112 (MQ=255)
                                               tGCCAGCAAGGCCGATTTCCAGCCCGCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTc                                                                                                                                   <  2:499672/113‑1 (MQ=255)
                                                 ccAGCAAGGCCGATTTCCAGCCCGCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCCGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTcc                                                                                            <  2:1064728/150‑1 (MQ=255)
                                                  cAGCAAGGCCGATTTCCAGCCCGCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCt                                                                                           <  2:1098593/150‑1 (MQ=255)
                                                        ggCCGATTTCCAGCCCGCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGt                                                                                     >  2:1627105/1‑150 (MQ=255)
                                                        ggCCGATTTCCAGCCCGCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCGAGCCGGGGCGttctggg                                                                                                                                >  2:540734/1‑102 (MQ=39)
                                                        ggCCGATTTCCAGCCCGCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCGAGCCGGGGCGttctggg                                                                                                                                <  1:540734/107‑6 (MQ=39)
                                                           cGATTTCCAGCCCGCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCctct                                                                                                               >  1:1114501/1‑121 (MQ=255)
                                                           cGATTTCCAGCCCGCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCctct                                                                                                               <  2:1114501/121‑1 (MQ=255)
                                                             aTTTCCAGCCCGCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGTTCcgc                                                                                <  1:307294/150‑1 (MQ=255)
                                                                tCCAGCCCGCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGTTCCGCGGt                                                                             <  2:474692/150‑1 (MQ=255)
                                                                  cAGCCCGCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGCGAACCCGCTCCTACAGGTTCCGCGGTAg                                                                           >  2:670255/1‑150 (MQ=255)
                                                                    gCCCGCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGTTCCGCGGTAGGa                                                                         <  1:1627105/150‑1 (MQ=255)
                                                                        gCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGTTCCGCGGTAGGAGCgg                                                                     <  2:1263455/150‑1 (MQ=255)
                                                                         cAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCNCGGGTGAACCCGCTCCTACAGGTTCCGCGGTAGGAGCggg                                                                    <  1:1614231/150‑1 (MQ=255)
                                                                         cAGCGCTGCGTCAGTCGGGTATTCCCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGTTCCGCGGTAGGAGCggg                                                                    <  2:967755/150‑1 (MQ=255)
                                                                                              ttCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCt                                                                                                                           >  2:1257670/1‑74 (MQ=255)
                                                                                              ttCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCt                                                                                                                           <  1:1257670/74‑1 (MQ=255)
                                                                                               tCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAAcc                                                                                                  <  1:926745/98‑1 (MQ=255)
                                                                                               tCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAAcc                                                                                                  >  2:926745/1‑98 (MQ=255)
                                                                                                           aaTCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGTTCCGCGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGggcaa                                            >  2:1087711/1‑140 (MQ=255)
                                                                                                           aaTCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGTTCCGCGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGggcaa                                            <  1:1087711/140‑1 (MQ=255)
                                                                                                                       ccGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGTTCCGCGGTAGGAGCgg                                                                     >  2:1381571/1‑103 (MQ=255)
                                                                                                                       ccGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGTTCCGCGGTAGGAGCgg                                                                     <  1:1381571/103‑1 (MQ=255)
                                                                                                                            tgATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGTTCCGCGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCAAGGCAATCAACCTCCTGAATAc                       <  2:111390/144‑1 (MQ=255)
                                                                                                                            tgATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGTTCCGCGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCAAGGCAATCAACCTCCTGAATAc                       >  1:111390/1‑144 (MQ=255)
                                                                                                                               tCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACa                                                                                        >  1:1633444/1‑76 (MQ=255)
                                                                                                                               tCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACa                                                                                        <  2:1633444/76‑1 (MQ=255)
                                                                                                                                    gaaccGCCCCGGCGAGCCGGGGCG‑TTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGTTCCGCGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCAAGGCAATCAACCTCCTGAATACCTCTCCAAAccccc         <  2:1214127/145‑1 (MQ=38)
                                                                                                                                    gaaaCGCCCCGGCGAGCCGGGGCG‑TTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGTTCCGCGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCAAGGCAATCAACCTCCTGAATACCTCTCCAAAccccc         >  1:1214127/3‑149 (MQ=38)
                                                                                                                                           cccGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACa                                                                                        >  1:1024963/1‑64 (MQ=255)
                                                                                                                                           cccGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACa                                                                                        <  2:1024963/64‑1 (MQ=255)
                                                                                                                                           cccGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGTTCCGCGGTAGGAGCgg                                                                     >  2:766260/1‑83 (MQ=255)
                                                                                                                                           cccGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGTTCCGCGGTAGGAGCgg                                                                     <  1:766260/83‑1 (MQ=255)
                                                                                                                                           cccGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGTTCCGCGGTAGGAGCGGGTTCACCcgc                                                           >  2:1219060/1‑93 (MQ=255)
                                                                                                                                           cccGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGTTCCGCGGTAGGAGCGGGTTCACCcgc                                                           <  1:1219060/93‑1 (MQ=255)
                                                                                                                                           cccGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGTTCCGCGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCAAGGCAATCAACCTCCTGAATACCTCTCCAAACCCCCGCCCCAg  >  1:993072/1‑150 (MQ=255)
                                                                                                                                            ccGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCgg                                                                                                         <  2:421580/46‑1 (MQ=255)
                                                                                                                                            ccGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCgg                                                                                                         >  1:421580/1‑46 (MQ=255)
                                                                                                                                                 tCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGTTCCGCGGTa                                                                            <  1:180672/70‑1 (MQ=255)
                                                                                                                                                 tCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGTTCCGCGGTa                                                                            >  2:180672/1‑70 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CTTGGGATGAAGAACGAAGAGAAACGTGCCTACGACTTCGGCTTCAGTGCCAGCAAGGCCGATTTCCAGCCCGCAGCGCTGCGTCAGTCGGGTATTCGCTAAGCTGAAATCACTGAACGCCGTGTGATCCCCAGAACGCCCCGGCTCGCCGGGGCGTTTCTATTTCCTGACCCGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGTTCCGCGGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGGCAAGGCAATCAACCTCCTGAATACCTCTCCAAACCCCCGCCCCAG  >  NC_002947/883664‑883952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑