Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 944,192 C→G 7.7% S478T (AGC→ACC)  PP_0806 ← surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947944,1920CG 7.7% 159.9 / 4.7 52S478T (AGC→ACC) PP_0806surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base C (24/24);  new base G (2/2);  total (26/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

CACACGGCTGCCAGCCGGCGCGGTACCGGTGAGGGTGGTGCCATCAGCATCGATGGCCAGATTGCTCGGGCTGTCGGGCGCGGTGGTCAGTGGTACATCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCAGGGCCAGGCC  >  NC_002947/944044‑944340
                                                                                                                                                    |                                                                                                                                                    
cacaCGGCTGCCAGCCGGCGCGGTACCGGTGAGGGTGGTGCCATCAGCATCGATGGCCAGATTGCTCGGGCTGTCGGGCGCGGTGGTCAGTGGTACATCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCt                                                                                                                                                     <  2:164675/150‑1 (MQ=35)
 acacGGCTGCCAGCCGGCGCGGTACCGGTGAGGGTGGTGCCATCAGCATCGATGGCCAGATTGCTCGGGCTGTCGGGCGCGGGGGTCAGTGGTACATCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTc                                                                                                                                                    >  2:879158/1‑150 (MQ=25)
   acGGCTGCCAGCCGGCGCGGTACCGGTGAGGGTGGTGCCATCAGCATCGATGGCCAGATTGCTCGGGCTGTTGGGCGCGGTGGTCAGTGGTACATCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCaa                                                                                                                                                  <  1:1180241/150‑1 (MQ=21)
   acGGCTGCCAGCCGGCGCGGTACCGGTGAGGGTGGTGCCATCAGCATCGATGGCCAGATTGCTCGGGCTGTCGGGCGCGGTGGTCAGTGGTACATCGTACTCGGTAGCCACTGAAGCATTACCTCTTGGGTCGGTCTGCAGCAGGCTCaa                                                                                                                                                  <  1:222007/150‑1 (MQ=25)
   acGGCTGCCAGCCGGCGCGGTACCGGTGAGGGTGGTGCCATCAGCATCGATGGCCAGATTGCTCGGGCTGTCGGGCGCGGTGGTCAGTGGTACATCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGGCGGTCTGCACCAGGCTCaa                                                                                                                                                  >  2:1080169/1‑150 (MQ=25)
         gCCAGCCGGCGCGGTACCGGTGAGGGTGGTGCCATCAGCATCGATGGCCAGATTGCTCGGGCTGTCGGGCGCGGTGGTCAGTGGTACATCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTc                                                                                                                                            <  1:763738/150‑1 (MQ=35)
                ggCGCGGTACCGGTGAGGGTGGTGCCATCAGCATCGATGGCCAGATTGCTCGGGCTGTCGGGCGCGGTGGTCAGTGGTACATCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGc                                                                                                                                     <  1:392345/150‑1 (MQ=35)
                       tACCGGTGAGGGTGGTGCCATCAGCATCGATGGCCAGATTGCTCGGGCTGTCGGGCGCGGTGGTCAGTGGTACATCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCgg                                                                                                                              <  1:1080169/150‑1 (MQ=35)
                             tGAGGGTAGTGCCGTCAGCATCGATGGCCAGATTGCTCGGGCTGTCGGGCGCGGTGGTCAGTGGTACATCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTgggg                                                                                                                        <  2:441850/150‑1 (MQ=255)
                              gAGGGTGGTGCCATCAGCATCGATGGCCAGATTGCTCGGGCTGTCGGGCGCGGTGGTCAGTGGTACATCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGc                                                                                                                       >  2:245015/1‑150 (MQ=35)
                                     gtgCCATCAGCATCGATGGCCAGATTGCTCGGGCTGTCGGGCGCGGTGGTCAGTGGTACATCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCg                                                                                                                >  1:1419143/1‑150 (MQ=39)
                                              gCATCGATGGCCAGATTGCTCGGGCTGTCGGGCGCGGTGGTCAGTGGTACATCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCCGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAg                                                                                                       >  2:1095033/1‑150 (MQ=255)
                                                      ggCCAGATTGCTCGGGCTGTCGGGCGCGGTGGTCAGTGGTACATCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCCGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGtt                                                                                               <  1:1095033/150‑1 (MQ=255)
                                                          aGATTGCTCGGGCTGTCGGGCGCGGTGGCCAGTGGTACATCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCa                                                                                           >  1:299684/1‑150 (MQ=255)
                                                            aTTGCTCGGGCTGTCGGGCGCGGTGGTCAGTGGTACATCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCAcc                                                                                         <  1:777755/150‑1 (MQ=38)
                                                               gCTCGGGCTGTCGGGCGCGGTGGTCAGTGGTACATCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGc                                                                                                                                <  2:1073901/108‑1 (MQ=38)
                                                               gCTCGGGCTGTCGGGCGCGGTGGTCAGTGGTACATCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGc                                                                                                                                >  1:1073901/1‑108 (MQ=38)
                                                               gCTCGGGCTGTCGGGCGCGGTGGTCAGTGGTACATCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCAc                                                                                      <  1:1644859/150‑1 (MQ=38)
                                                                 tCGGGCTGTCGGGCGCGGTGGTCAGTGGTACATCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCaa                                                                                                                                                  >  1:976190/1‑88 (MQ=39)
                                                                 tCGGGCTGTCGGGCGCGGTGGTCAGTGGTACATCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCaa                                                                                                                                                  <  2:976190/88‑1 (MQ=39)
                                                                 tCGGGCTGTCGGGCGCGGTGGTCAGTGGTACATCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGa                                                                                    >  1:1062615/1‑150 (MQ=39)
                                                                             gcgcGGTGGTCAGTGGTACATCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGa                                                                        <  1:245015/150‑1 (MQ=38)
                                                                                      tCAGTGGTACATCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGa                                                                                    >  2:1428415/1‑129 (MQ=40)
                                                                                      tCAGTGGTACATCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGa                                                                                    <  1:1428415/129‑1 (MQ=40)
                                                                                                 tCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGtt                                                                                               >  2:899195/1‑107 (MQ=40)
                                                                                                 tCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGtt                                                                                               <  1:899195/107‑1 (MQ=40)
                                                                                                 tCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATcg                                                    >  1:327828/1‑150 (MQ=255)
                                                                                                           tAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGa                                          <  1:879158/150‑1 (MQ=255)
                                                                                                              ccACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGa                                                                                                                     >  2:1131601/1‑72 (MQ=255)
                                                                                                              ccACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGa                                                                                                                     <  1:1131601/72‑1 (MQ=255)
                                                                                                              ccACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAg                                       >  1:1389537/1‑150 (MQ=255)
                                                                                                                        aTTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTgg                                                                                                                          <  1:244420/57‑1 (MQ=38)
                                                                                                                        aTTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTgg                                                                                                                          >  2:244420/1‑57 (MQ=35)
                                                                                                                         ttgccgctTGGGTCGGTCTGCACCAGGGTCAACTGCTCGCCCGGCTGCGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCTCCCACAACACCCGTGCCGATCAcggcgccactgttgaggtgcgcgatgccagtggcaccgt                              >  1:874587‑M2/4‑109 (MQ=255)
                                                                                                                         ttGCCGCTTGGGTCGGTCTGCACCAGGGTCAACTGCTCGCCCGGCTGCGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCTCCCACAACACCCGTGCCGATCAcggcgccactgttgaggtgcgcgatgccagtggcaccgt                              <  2:874587‑M2/148‑40 (MQ=255)
                                                                                                                          tACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGt                                                                             >  2:1086409/1‑100 (MQ=37)
                                                                                                                          tACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGt                                                                             <  1:1086409/100‑1 (MQ=35)
                                                                                                                          tACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCt                           >  1:216510/1‑150 (MQ=255)
                                                                                                                          tACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCt                           <  2:216510/150‑1 (MQ=255)
                                                                                                                                     tcggtcTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCg                >  1:870195/1‑150 (MQ=255)
                                                                                                                                        gtcTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGt                                                                                                <  1:23709/67‑1 (MQ=37)
                                                                                                                                        gtcTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGt                                                                                                >  2:23709/1‑67 (MQ=37)
                                                                                                                                        gtcTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTc             >  1:1063046/1‑150 (MQ=255)
                                                                                                                                         tcTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCa            >  1:1377240/1‑150 (MQ=255)
                                                                                                                                         tcTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCa            >  2:346195/1‑150 (MQ=255)
                                                                                                                                         tcTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCa            >  2:394768/1‑150 (MQ=255)
                                                                                                                                                cAGGGTCAACTGCTCGCCCGGCTGCGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCTCCCACAACACCCGTGCCGATCAcggcgccactgttgaggtgcgcgatgccagtggcaccgtgatcggcacgggtgttgtg           >  1:1359512‑M2/1‑86 (MQ=255)
                                                                                                                                                cAGGGTCAACTGCTCGCCCGGCTGCGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCTCCCACAACACCCGTGCCGATCAcggcgccactgttgaggtgcgcgatgccagtggcaccgtgatcggcacgggtgttgtg           <  2:1359512‑M2/144‑59 (MQ=255)
                                                                                                                                                cAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCAGGGCCAg     <  2:299684/150‑1 (MQ=255)
                                                                                                                                                cAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCAGGGCCAg     >  2:159098/1‑150 (MQ=255)
                                                                                                                                                cAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCAGGGCCAg     <  2:1063046/150‑1 (MQ=255)
                                                                                                                                                   gCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCAGGGCCAGGcc  <  2:1389537/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CACACGGCTGCCAGCCGGCGCGGTACCGGTGAGGGTGGTGCCATCAGCATCGATGGCCAGATTGCTCGGGCTGTCGGGCGCGGTGGTCAGTGGTACATCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCAGGGCCAGGCC  >  NC_002947/944044‑944340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑