Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 944,257 G→A 8.3% V456V (GTC→GTT)  PP_0806 ← surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947944,2570GA 8.3% 133.1 / 3.0 48V456V (GTC→GTT) PP_0806surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base G (27/17);  new base A (2/2);  total (29/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01

TCGGGCTGTCGGGCGCGGTGGTCAGTGGTACATCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCAGGGCCAGGCCGTCAGCACCAACTGTAATATTGGTTACTGCGTCAGGCGGTGTGATGTCCGGCGCATCGAA  >  NC_002947/944109‑944400
                                                                                                                                                    |                                                                                                                                               
tCGGGCTGTCGGGCGCGGTGGTCAGTGGTACATCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGa                                                                                                                                                >  1:1062615/1‑150 (MQ=39)
            gcgcGGTGGTCAGTGGTACATCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGa                                                                                                                                    <  1:245015/150‑1 (MQ=38)
                     tCAGTGGTACATCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGa                                                                                                                                                >  2:1428415/1‑129 (MQ=40)
                     tCAGTGGTACATCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGa                                                                                                                                                <  1:1428415/129‑1 (MQ=40)
                                tCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATcg                                                                                                                >  1:327828/1‑150 (MQ=255)
                                          tAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGa                                                                                                      <  1:879158/150‑1 (MQ=255)
                                             ccACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAg                                                                                                   >  1:1389537/1‑150 (MQ=255)
                                                        ttgccgctTGGGTCGGTCTGCACCAGGGTCAACTGCTCGCCCGGCTGCGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCTCCCACAACACCCGTGCCGATCAcggcgccactgttgaggtgcgcgatgccagtggcaccgt                                                                                          >  1:874587‑M2/4‑109 (MQ=255)
                                                        ttGCCGCTTGGGTCGGTCTGCACCAGGGTCAACTGCTCGCCCGGCTGCGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCTCCCACAACACCCGTGCCGATCAcggcgccactgttgaggtgcgcgatgccagtggcaccgt                                                                                          <  2:874587‑M2/148‑40 (MQ=255)
                                                         tACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGt                                                                                                                                         <  1:1086409/100‑1 (MQ=35)
                                                         tACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGt                                                                                                                                         >  2:1086409/1‑100 (MQ=37)
                                                         tACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCt                                                                                       <  2:216510/150‑1 (MQ=255)
                                                         tACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCt                                                                                       >  1:216510/1‑150 (MQ=255)
                                                                    tcggtcTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCg                                                                            >  1:870195/1‑150 (MQ=255)
                                                                       gtcTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTc                                                                         >  1:1063046/1‑150 (MQ=255)
                                                                        tcTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCa                                                                        >  1:1377240/1‑150 (MQ=255)
                                                                        tcTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCa                                                                        >  2:394768/1‑150 (MQ=255)
                                                                        tcTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCa                                                                        >  2:346195/1‑150 (MQ=255)
                                                                               cAGGGTCAACTGCTCGCCCGGCTGCGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCTCCCACAACACCCGTGCCGATCAcggcgccactgttgaggtgcgcgatgccagtggcaccgtgatcggcacgggtgttgtg                                                                       >  1:1359512‑M2/1‑86 (MQ=255)
                                                                               cAGGGTCAACTGCTCGCCCGGCTGCGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCTCCCACAACACCCGTGCCGATCAcggcgccactgttgaggtgcgcgatgccagtggcaccgtgatcggcacgggtgttgtg                                                                       <  2:1359512‑M2/144‑59 (MQ=255)
                                                                               cAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCAGGGCCAg                                                                 >  2:159098/1‑150 (MQ=255)
                                                                               cAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCAGGGCCAg                                                                 <  2:299684/150‑1 (MQ=255)
                                                                               cAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCAGGGCCAg                                                                 <  2:1063046/150‑1 (MQ=255)
                                                                                  gCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCAGGGCCAGGcc                                                              <  2:1389537/150‑1 (MQ=255)
                                                                                    tCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCAGGGCCAGGCCGt                                                            >  1:68086/1‑150 (MQ=255)
                                                                                      aaCTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCAGGGCCAGGCCGTCa                                                          >  2:333680/1‑150 (MQ=255)
                                                                                      aaCTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCAGGGCCAGGCCGTCa                                                          >  1:719139/1‑150 (MQ=255)
                                                                                        cTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCAGGGCCAGGCCGTCAGc                                                        >  1:296110/1‑150 (MQ=255)
                                                                                                   gCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCa                                                                        <  2:1375037/123‑1 (MQ=255)
                                                                                                   gCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCa                                                                        >  1:1375037/1‑123 (MQ=255)
                                                                                                        cacagccgggCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCAGGGCCAGGCCGTCAGCACCAACTGTAATATTg                                        <  2:320494/143‑1 (MQ=255)
                                                                                                         cGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGAGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCAGGGCCAGGCCGTCAGCACCAACTGTAATAtttg                                       >  2:1212998/1‑148 (MQ=255)
                                                                                                             tGGGGCGAGGTCTATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCAGGGCCAGGCCGTCAGCACCAACTGTAATATTGGTTAc                                   <  2:327828/150‑1 (MQ=255)
                                                                                                               gggCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCAGGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCAGGGCCAGGCCGTCAGCACCAACTGTAATATTGGTTACTg                                 >  2:539432/1‑150 (MQ=255)
                                                                                                                       tCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCa                                                                                                                   >  1:1207431/1‑60 (MQ=255)
                                                                                                                       tCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCa                                                                                                                   <  2:1207431/60‑1 (MQ=255)
                                                                                                                         gATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCAGGGCCAGGCCGTCAGCACCAACTGTAATATTGGTTACTGCGTCAGGCgg                       >  1:942948/1‑150 (MQ=255)
                                                                                                                            cAGGAAGGTGCCGGTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGCCTCGCCGCGACCGCTCAGGGCCAGGCCGTCAGCACCAACTGTAATATTGGTTACTGCGTCAGGCGgtgt                    <  2:649507/150‑1 (MQ=255)
                                                                                                                             aGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCAGGGCCAGGCCGTCAGCACCAACTGTAATATTGGTTACTGCGTCAGGCGGtgtg                   >  1:656462/1‑150 (MQ=255)
                                                                                                                               gAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCAGGGCCAGGCCGTCAGCACCAACTGTAATATTGGTTACTGCGTCAGGCGGTGTGAt                 <  2:1062615/150‑1 (MQ=255)
                                                                                                                                     gCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCAGGGCCAGGCCGTCAGCACCAACTGTAATATTGGTTACTGCGTCAGGCGGTGTGATGTCCgg           >  1:195449/1‑150 (MQ=255)
                                                                                                                                      ccGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCAGGGCCAGGCCGTCAGCACCAACTGTAATATTGGTTACTGCGTCAGGCGGTGTGATGTCCGgc          <  1:333680/150‑1 (MQ=255)
                                                                                                                                       cGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCAGGGCCAGGCCGTCAGCACCAACTGTAatat                                          >  2:27178/1‑117 (MQ=255)
                                                                                                                                       cGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCAGGGCCAGGCCGTCAGCACCAACTGTAatat                                          <  1:27178/117‑1 (MQ=255)
                                                                                                                                           ggCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCAGGGCCAGGCCGTCAGCACCAACTGTAATATTGGTTACTGCGTCAGGCGGTGTGATGTCCGGCGCATc     <  2:870195/150‑1 (MQ=255)
                                                                                                                                            gCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCAGGGCCAGGCCGTCAGCACCAACTGTAATATTGGTTACTGCGTCAGGCGGTGTGATGTCCGGCGCATCg    >  2:1338918/1‑150 (MQ=255)
                                                                                                                                              acacACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACTGCTCAGGGCCAGGCCGTCAGCACCAACTGTAATATTGGTTACTGCGTCAGGCGGTGTGATGTCCGGCGCATCGaa  >  2:708786/4‑150 (MQ=255)
                                                                                                                                              aCCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCAGGGCCAGGCCGTCAGCACCAACTGTAATATTGGTTACTGCGTCAGGCGGTGTGATGTCCGGCGCATCGaa  >  2:1563959/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
TCGGGCTGTCGGGCGCGGTGGTCAGTGGTACATCGTACTCGGTAGCCACTGAAGCATTACCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTGGGGCGAGGTCGATCAGGAAGGTGCCGTTGGCACCCACGACACCCGTGCCGATCACAGTGCCATTGGCATCGCGCACTTCGACAGTGGCACCTGGCTCGCCGCGACCGCTCAGGGCCAGGCCGTCAGCACCAACTGTAATATTGGTTACTGCGTCAGGCGGTGTGATGTCCGGCGCATCGAA  >  NC_002947/944109‑944400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑