Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 956,587 A→T 9.3% intergenic (+17/‑97) alaC → / → PP_0818 aminotransferase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947956,5870AT 9.3% 154.2 / 8.1 54intergenic (+17/‑97)alaC/PP_0818aminotransferase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (24/25);  new base T (2/3);  total (26/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.28e-01

CGGCTTTGGTGACTATGGGGACGACCATGTGCGCTTTGCGTTGATCGAGAACCAGGACCGTATTCGCCAGGCCATCCGCGGGATCCGGCAAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTACGCCTACCGTCAGCACTCATTTCTCACCAAAGAGATCCCCCATGAGTGTCTTCACCGCCTACTTCTGCGGCACTGGCTCGCACCGTTTC  >  NC_002947/956447‑956731
                                                                                                                                            |                                                                                                                                                
cGGCTTTGGTGACTATGGGGACGACCATGTGCGCTTTGCGTTGATCGAGAACCAGGACCGTATTCGCCAGGCCATCCGCGGGATCCGGCAAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGcccc                                                                                                                                         <  2:1217586/150‑1 (MQ=255)
 ggCTTTGGTGACTATGGGGACGACCATGTGCGCTTTGCGTTGATCGAGAACCAGGACCGTATTCGCCAGGCCATCCGCGGGATCCGGCAAATGTTACGGGCTGATGGTTTGGCTCGCAAGTAAGGCCAATGGGGCTGGGAAGCAGCCGCa                                                                                                                                        >  2:818563/1‑150 (MQ=255)
  gCTTTGGTGACTATGGGGACGACCATGTGCGCTTTGCGTTGATCGAGAACCAGGACCGTATTCGCCAGGCCATCCGCGGGATCCGGCAAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCaa                                                                                                                                       >  2:117458/1‑150 (MQ=255)
    tttGGTGACTATGGGGACGACCATGTGCGCTTTGCGTTGATCGAGAACCAGGACCGTATTCGCCAGGCCATCCGCGGGATCCGGCAAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCTTCGCAGCCCCattg                                                                                                                                     <  1:305044/150‑4 (MQ=255)
             tATGGGGACGACCATGTGCGCTTTGCGTTGATCGAGAACCAGGACCGTATTCGCCAGGCCATCCGCGGGATCCGGCAAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTgg                                                                                                                            <  2:479171/150‑1 (MQ=255)
             tATGGGGACGACCATGTGCGCTTTGCGTTGATCGAGAACCAGGACCGTATTCGCCAGGCCATCCGCGGGATCCGGCAAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTgg                                                                                                                            >  1:602561/1‑150 (MQ=255)
                 gggACGACCACGTGCGCTTTGCGTTGAGCGAGAACCAGGACCGTATTCGCCAGGCCATCCGCGGGATCCGGCAAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGa                                                                                                                        <  2:288823/150‑1 (MQ=255)
                  ggACGACCATGTGCGCTTTGCTTTGATCGAGAACCAGGACCGTATTCGCCAGGCCATCCGCGGGATCCGGCAAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAg                                                                                                                       >  1:131397/1‑150 (MQ=255)
                  ggACGACCATGTGCGCTTTGCGTTGATCGAGAACCAGGACCGTATTCGCCAGGCCATCCGCGGGATCCGGCAAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAg                                                                                                                       <  2:517705/150‑1 (MQ=255)
                    acgacCATGTGCGCTTTGCGTTGATCGAGAACCAGGACCGTATTCGCCAGGCCATCCGCGGGATCCGGCAAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCt                                                                                                                     >  1:923660/1‑150 (MQ=255)
                        ccACGTGCGCTTTGCGTTGATCGAGAACCAGGACCGTATTCGCCAGGCCATCCGCGGGATCCGGCAAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTcc                                                                                                                 <  1:628342/150‑1 (MQ=255)
                                  tttGCGTTGATTCAGAACCAGGACCGTTTTCGCCAGGCCATCCGCGGGATCCGGCAAATTTTCCGGGCTGATGGTTTGGCTCTCAAGTTAGCCCAATTGGGGTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTc                                                                                                       <  2:389907/150‑1 (MQ=255)
                                      cGTTGATCGAGAACCAGGACCGTATTCGCCAGGCCATCCGCGGGATCCGGCAAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGt                                                                                                   >  2:1434208/1‑150 (MQ=255)
                                        ttGATCGAGAACCAGGACCGTATTCGCCAGGCCATCCGCGGGATCCGGCAAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTa                                                                                                 >  1:914913/1‑150 (MQ=255)
                                          gATCGAGAACCAGGACCGTATTCGCCAGGCCATCCGCGGGATCCGGCAAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTAcc                                                                                               >  2:718396/1‑150 (MQ=255)
                                            tCGAGAACCAGGACCGTATTCGCCAGGCCATCCGCGGGATCCGGCAAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCTTCGCAGCCCCattggg                                                                                                                                   <  2:864858/112‑6 (MQ=255)
                                            tCGAGAACCAGGACCGTATTCGCCAGGCCATCCGCGGGATCCGGCAAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCTTCGCAGCCCCattggg                                                                                                                                   >  1:864858/1‑107 (MQ=255)
                                            tCGAGAACCAGGACCGTATTCGCCAGGCCATCCGCGGGATCCGGCAAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCg                                                                                             >  1:858076/1‑150 (MQ=255)
                                               agaACCAGGACCGTATTCGCCAGGCCATCCGCGGGATCCGGCAAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTAc                                                                                          >  2:791221/1‑150 (MQ=255)
                                                 aaCCAGGACCGTATTCTCCAGTCCATCCGCGGGATCCGTCAAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTACGc                                                                                        <  1:818563/150‑1 (MQ=255)
                                                  aCCAGGACCGTATTCGCCAGGCCATCCGCGGGATCCGGCAAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCct                                                                                                                                 <  2:1180277/108‑1 (MQ=255)
                                                  aCCAGGACCGTATTCGCCAGGCCATCCGCGGGATCCGGCAAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCct                                                                                                                                 >  1:1180277/1‑108 (MQ=255)
                                                    cAGGACCGTATTCGCCAGGCCATCCGCGGGATCCGGCAAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGt                                                                                                                          >  1:845233/1‑113 (MQ=255)
                                                    cAGGACCGTATTCGCCAGGCCATCCGCGGGATCCGGCAAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGt                                                                                                                          <  2:845233/113‑1 (MQ=255)
                                                    cAGGACCGTATTCGCCAGGCCATCCGCGGGATCCGGCAAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTc                                                                                                       <  2:1375862/132‑1 (MQ=255)
                                                    cAGGACCGTATTCGCCAGGCCATCCGCGGGATCCGGCAAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTc                                                                                                       >  1:1375862/1‑132 (MQ=255)
                                                         ccGTATTCGCCAGGCCATCCGCGGGATCCGGCAAACGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTACGCCTACCGTc                                                                                <  1:990146/150‑1 (MQ=255)
                                                           gTATTCGCCAGGCCATCCGCGGGATCCGGCAAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTACGCCTACCGTCAg                                                                              <  2:914913/150‑1 (MQ=255)
                                                             aTTCGCCAGGCCATCCGCGGGATCCGGCAAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAAc                                                                                                                                      >  2:1272237/1‑92 (MQ=255)
                                                             aTTCGCCAGGCCATCCGCGGGATCCGGCAAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAAc                                                                                                                                      <  1:1272237/92‑1 (MQ=255)
                                                                         aTCCGCGGGATCCGGCAAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTACGCCTACCGTCAGCACTCATTTCTCAc                                                                >  2:400529/1‑150 (MQ=255)
                                                                         aTCCGCGGGATCCGGCAAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTACGCCTACCGTCAGCACTCATTTCTCAc                                                                >  1:727447/1‑150 (MQ=255)
                                                                             gcgGGATCCGGCAAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTACGCCTACCGTCAGCACTCATTTTTCatgaaa                                                            >  2:220707/1‑145 (MQ=255)
                                                                              cgGGATCCGGCAAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTACGCCTACCGTCAGCACTCATTTCTCACCAAag                                                           <  1:117458/150‑1 (MQ=255)
                                                                                 gATCCGGCAAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTACGCCTACCGTCAGCACTCATTTCTCACCAAAgaga                                                        <  2:168991/150‑1 (MQ=255)
                                                                                     cGGCAAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTACGCCTACCGTCAGCACTCATTTCTCACCAAAGAGATccc                                                    >  2:238144/1‑150 (MQ=255)
                                                                                      ggCAAATGTTCCGGGCTGATGGTTTGGCTCTCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTTGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTACGCCTACCGTCCGCACTCATTTCTCACCAAAAAAATcccc                                                   >  2:497631/1‑150 (MQ=255)
                                                                                        cAAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTACGCCTACCGTCAGCACTCATTTCTCACCAAAGAGATCCCCCa                                                 <  1:718396/150‑1 (MQ=255)
                                                                                         aaaTGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCTTCGCAGCCCCattg                                                                                                                                     <  1:1169975/65‑4 (MQ=25)
                                                                                         aaaTGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCTTCGCAGCCCCattg                                                                                                                                     >  2:1169975/1‑62 (MQ=25)
                                                                                           aTGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTACGCCTACCGTCAGCACTCATTTCTCACCAAAGAGATCCCCCATGa                                              <  1:1394579/150‑1 (MQ=255)
                                                                                                ccGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTACTCCTACCGTCAGCACTCATTTCTCACCAAAGATATCCCCCATGAGTGTc                                         <  2:602561/150‑1 (MQ=255)
                                                                                                        aTGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTACGCCTACCGTCAGCACTCATTTCTCACCAAAGAGATCCCCCATGAGTGTCTTCACCGc                                 >  1:986895/1‑150 (MQ=255)
                                                                                                            tttGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGATCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTACGCCTACCGTCAGCACTCATTTCTCACCAAAGAGATCCCCCATGAGTGTCTTCACCGCCTAc                             <  1:429545/150‑1 (MQ=255)
                                                                                                                  tCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTACGCCTACCGTCAGCACTCATTTCTCACCAAAGAGATCCCCCATGAGTGTCTTCACCGCCTACTTCTGc                       >  1:1218453/1‑150 (MQ=255)
                                                                                                                   cGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTACGCCTACCGTCAGCACTCATTTCTCACCAAAGAGATCCCCCATGAGTGTCTTCACCGCCTACTTCTGCg                      <  2:986895/150‑1 (MQ=255)
                                                                                                                    gCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTACGCCTACCGTCAGCACTCATTTCTCACCAAAGAGATCCCCCATGAGTGTCTTCa                                     <  2:674316/134‑1 (MQ=255)
                                                                                                                    gCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTACGCCTACCGTCAGCACTCATTTCTCACCAAAGAGATCCCCCATGAGTGTCTTCa                                     >  1:674316/1‑134 (MQ=255)
                                                                                                                          aaGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTACGCCTACCGTCAGCACTCATTTCTCACCAAAGAGATCCCCCATGAGTGTCTTCACCGCCTACTTCTGCGGCACTgg               <  1:220707/150‑1 (MQ=255)
                                                                                                                             cccAATGGGGCTGCGAAGCAGCGCCAACTGCCTCTTGGGAGAGCTTTCCGCCTGCCTTCTGGTTACCCGTACGCCTACCGTCAGCACTCATTTCTCACCAAAGAGATCCCCCATGAGTGTCTTCACCGCATACTTCTGCGGCCCTGGCTc            >  2:605703/1‑150 (MQ=255)
                                                                                                                                  tGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTATGTTCCCCGTCATCACTCATTTCTCACCAAAGAGATCCCCCATGAGTGTCTTCACCGCCTTCTTCTGAGGCACTGGCTCGCAcc       <  2:131397/150‑1 (MQ=255)
                                                                                                                                       cTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTACGCCTACCGTCAGCACTCATTTCTCACCAAAGAGATCCCCCATGAGTGTCTTCACCGCCTACTTCTGCGGCACTGGCTCGCACCGTTTc  <  1:791221/150‑1 (MQ=255)
                                                                                                                                           gAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTACGCCTACCGTCa                                                                               <  1:24125/69‑1 (MQ=255)
                                                                                                                                           gAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTACGCCTACCGTCa                                                                               >  2:24125/1‑69 (MQ=255)
                                                                                                                                            |                                                                                                                                                
CGGCTTTGGTGACTATGGGGACGACCATGTGCGCTTTGCGTTGATCGAGAACCAGGACCGTATTCGCCAGGCCATCCGCGGGATCCGGCAAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTACGCCTACCGTCAGCACTCATTTCTCACCAAAGAGATCCCCCATGAGTGTCTTCACCGCCTACTTCTGCGGCACTGGCTCGCACCGTTTC  >  NC_002947/956447‑956731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑