Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,049,192 C→A 6.5% L137L (CTG→CTT)  PP_0907 ← RND transporter membrane fusion protein‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,049,1920CA 6.5% 241.6 / 3.6 76L137L (CTG→CTT) PP_0907RND transporter membrane fusion protein‑like protein
Reads supporting (aligned to +/- strand):  ref base C (33/38);  new base A (3/2);  total (36/40)
Fisher's exact test for biased strand distribution p-value = 6.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.31e-01

TGCGTTTCTTGATCTTCTGCGCCTTAAGCTGCTCGACCACCGCCTGGTTGCGCCGGTACTGGGAGGAAAGGCGGTCGAACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGTTGCCCGGCCTTGACCTGTTGGCCTGAGTCGAAGTGCAGGGATTTGACGATGCCTTCCACCTCCAGGCTCAGATCCACGCCCTGCAG  >  NC_002947/1049044‑1049341
                                                                                                                                                    |                                                                                                                                                     
tGCGTTTCTTGATCTTCTGCGCCTTAAGCTGCTCGACCACCGCCTGGTTGCGCCGGTACTGGGAGGAAAGGCGGTCGAACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCa                                                                                                                                                      >  1:842270/1‑150 (MQ=255)
    tttCTTGATCTTCTGCGCCTTAAGCTGCTCGACCACCGCCTGGTTGCGCCGGTACTGGGAGGAAAGGCGGTCGAACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTc                                                                                                                                                  >  1:1467020/1‑150 (MQ=255)
          gATCTTCTGCGCCTTAAGCTGCTCGACCACCGCCTGGTTGCGCCGGTACTGGGAGGAAAGGCGGTCGAACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAg                                                                                                                                            >  2:957000/1‑150 (MQ=255)
                 tGCGCCTTAAGCTGCTCGACCACCGCCTGGTTGCGCCGGTACTGGGAGGAAAGGCGGTCGAACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCTAGGCCCAGGTCGGCCAGGGCGGTa                                                                                                                                     >  1:1312804/1‑150 (MQ=255)
                 tGCGCCTTAAGCTGCTCGACCACCGCCTGGTTGCGCCGGTACTGGGAGGAAAGGCGGTCGAACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTa                                                                                                                                     >  2:1210029/1‑150 (MQ=255)
                     ccTTAAGCTGCTCGACCACCGCCTGGTTGCGCCGGTACTGGGAGGAAAGGCGGTCGAACTCGCCGCGGGAGATGGCAGACTCGCTAACCACCTTTCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGa                                                                                                                                 >  1:371732/1‑150 (MQ=255)
                         aaGCTGCTCGACCACCGCCTGGTTGCGCCGGTACTGGGAGGAAAGGAGGTCGAACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAg                                                                                                                             >  2:978617/1‑150 (MQ=255)
                             tgctCGACCACCGCCTGGTTGCGCCGGTACTGGGAGGAAAGGCGGTCGAACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCt                                                                                                                         <  1:543179/150‑1 (MQ=255)
                              gctCGACCACCGCCTGGTTGCGCCGGTACTGGGAGGAAAGGCGGTCGAACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTg                                                                                                                        <  2:842270/150‑1 (MQ=255)
                                   accaccGCCTGGTTGCGCCGGTACTGGGAGGAAAGGCGGTCGAACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTcc                                                                                                                   >  2:1254202/1‑150 (MQ=255)
                                    ccaccGCCTGGTTGCAGCGGTCCTGGGAGGAAAGGCGGTCGAACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCt                                                                                                                  <  1:424/150‑1 (MQ=255)
                                     caccGCCTGGTTGCGCCGGTACTGGGAGGAAAGGCGGTCGAACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTg                                                                                                                 >  1:167902/1‑150 (MQ=255)
                                       ccGCCTGGTTGCGCCGGTACTGGGAGGAAAGGCGGTCGAACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGAt                                                                                                               >  2:310782/1‑150 (MQ=255)
                                          ccTGGTTGCGCCGGTACTGGGAGGAAAGGCGGTCGAACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGCCCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAg                                                                                                                             >  1:1133128/1‑133 (MQ=255)
                                          ccTGGTTGCGCCGGTACTGGGAGGAAAGGCGGTCGAACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAg                                                                                                                             <  2:1133128/133‑1 (MQ=255)
                                           cTGGTTGCGCCGGTACTGGGAGGAAAGGCGGTCGAACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGAtcgtc                                                                                                           <  1:957000/150‑1 (MQ=255)
                                                tGCGCCGGTACTGGGAGGAAAGGCGGTCGAACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGa                                                                                                      <  2:712185/150‑1 (MQ=255)
                                                   gcCGGTACTGGGAGGAAAGGCGGTCGAACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCt                                                                                                   >  1:92411/1‑150 (MQ=255)
                                                   gcCGGTACTGGGAGGAAAGGCGGTCGAACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGACGTTGAGCt                                                                                                   >  1:20258/1‑150 (MQ=255)
                                                       gTACTGGGAGGAAAGGCGGTCGCACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCaa                                                                                               <  2:787280/150‑1 (MQ=255)
                                                         aCTGGGAGGAAAGGCGGTCGAACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAaga                                                                                             <  1:1498772/150‑1 (MQ=255)
                                                         aCTGGGAGGAAAGGCGGTCGAACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAaga                                                                                             <  1:236754/150‑1 (MQ=255)
                                                         aCTGGGAGGAAAGGCGGTCGAACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAaga                                                                                             <  2:371732/150‑1 (MQ=255)
                                                          cTGGGAGGAAAGGCGGTCGAACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCg                                                                                                                                        <  2:873997/106‑1 (MQ=255)
                                                          cTGGGAGGAAAGGCGGTCGAACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCg                                                                                                                                        >  1:873997/1‑106 (MQ=255)
                                                           tGGGAGGAAAGGCGGTCGAACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGc                                                                                           >  1:908830/1‑150 (MQ=255)
                                                            gggAGGAAAGGCGGTCGAACTCGCCGCGGGAGATGGCCGAATCGCCCACAAGATGTGTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCa                                                                                          >  2:964570/1‑150 (MQ=255)
                                                             ggaggaAAGGCGGTCGAACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAg                                                                                         >  1:1051740/1‑150 (MQ=255)
                                                              gaggaAAGGCGGTCGAACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTACTGATCGTCGTTGAGCTGCAAGAGCAGt                                                                                        >  1:539010/1‑150 (MQ=255)
                                                               aggaAAGGCGGTCGAACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGtt                                                                                       <  2:92411/150‑1 (MQ=255)
                                                                       cGGTCGAACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGTTGCCCGGcc                                                                               <  1:88071/150‑1 (MQ=255)
                                                                         gTCGAACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGTTGCCCGGCCtt                                                                             <  1:1523574/150‑1 (MQ=255)
                                                                            gAACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGAt                                                                                                               <  1:1047531/113‑1 (MQ=255)
                                                                            gAACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGAt                                                                                                               >  2:1047531/1‑113 (MQ=255)
                                                                                tCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGc                                                                                                                                               >  1:1122964/1‑77 (MQ=255)
                                                                                tCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGc                                                                                                                                               <  2:1122964/77‑1 (MQ=255)
                                                                                tCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGTTGCCCGGCCTTGAc                                                                          <  1:876767/146‑1 (MQ=255)
                                                                                tCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGTTGCCCGGCCTTGAc                                                                          >  2:876767/1‑146 (MQ=255)
                                                                                      cgGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCGGGTGCCCGGCCTTGACCTGTTGGCCt                                                                <  1:864092/150‑1 (MQ=255)
                                                                                      cgGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGTTGCCCGGCCTTGACCTGTTGGCCt                                                                <  1:340891/150‑1 (MQ=255)
                                                                                       gggAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGTTGCCCGGCCTTGACCTGTTGGCCTg                                                               >  1:1314054/1‑150 (MQ=255)
                                                                                        ggAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCCGCCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGTTGCCCGGCCTTGACCTGTTGGCCTGa                                                              <  1:216770/150‑1 (MQ=255)
                                                                                         gagaTGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGTTGCCCGGCCTTGACCTGTTGGCCTGAg                                                             <  1:1210029/150‑1 (MQ=255)
                                                                                              ggCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGTTGCCCGGCCTTGACCTGTTGGCCTGAGTCGaa                                                        <  2:629811/150‑1 (MQ=255)
                                                                                               gCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGt                                                                                                                       >  2:1376032/1‑86 (MQ=255)
                                                                                               gCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGt                                                                                                                       <  1:1376032/86‑1 (MQ=255)
                                                                                               gCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGAtc                                                                                                              <  2:1470850/95‑1 (MQ=255)
                                                                                               gCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGAtc                                                                                                              >  1:1470850/1‑95 (MQ=255)
                                                                                                     tCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAggg                                                                                                                                          >  1:631663/1‑61 (MQ=255)
                                                                                                     tCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAggg                                                                                                                                          <  2:631663/61‑1 (MQ=255)
                                                                                                        cccACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAggg                                                                                                                           <  1:181424/73‑1 (MQ=255)
                                                                                                        cccACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAggg                                                                                                                           >  2:181424/1‑73 (MQ=255)
                                                                                                             caGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGTTGCCCGGCCTTGACCTGTTGGCCTGAGTCGAAGTGCAg                                                  >  2:1553643/1‑141 (MQ=255)
                                                                                                             caGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGTTGCCCGGCCTTGACCTGTTGGCCTGAGTCGAAGTGCAg                                                  <  1:1553643/141‑1 (MQ=255)
                                                                                                                ctggctgCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGTTGCCCGGCCTTGACCTGTTGGCCTGAGTCGAAGTGCAGGGATTTGACGAt                                      <  1:310782/150‑1 (MQ=255)
                                                                                                                 tggctgCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAagag                                                                                            >  1:1619441/1‑95 (MQ=255)
                                                                                                                 tggctgCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAagag                                                                                            <  2:1619441/95‑1 (MQ=255)
                                                                                                                      gCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGTTGCCCGGCCTTGACCTGTTGGCCTGa                                                              <  1:1090322/120‑1 (MQ=255)
                                                                                                                      gCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGTTGCCCGGCCTTGACCTGTTGGCCTGa                                                              >  2:1090322/1‑120 (MQ=255)
                                                                                                                       ccACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGTTGCCCGGCCTTGACCTGTTGGCCTGAGTCGAAGTGCAGGGATTTGACGATGCCTTcc                               <  1:964570/150‑1 (MQ=255)
                                                                                                                         aCGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGTTGCCCGGCCTTGACCTGTTGGCCTGAGTCGAAGTGCAGGGATTTGACGATGCCTTCCAc                             <  1:1254202/150‑1 (MQ=255)
                                                                                                                              cctggccGACCTGGGCCTGGCCAAGGTCGACCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGTTGCCCGGCCTTGACCTGTTGGCCTGAGTCGAAGTGCa                                                   >  1:47436/7‑123 (MQ=38)
                                                                                                                              cctggccGACCTGGGCCTGGCCAAGGTCGACCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGTTGCCCGGCCTTGACCTGTTGGCCTGAGTCGAAGTGCa                                                   <  2:47436/117‑1 (MQ=38)
                                                                                                                              cGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGTTGCCCGGCCTTGACCTGTTGGCCTGAGTCGaa                                                        >  1:93712/1‑118 (MQ=255)
                                                                                                                              cGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGTTGCCCGGCCTTGACCTGTTGGCCTGAGTCGaa                                                        <  2:93712/118‑1 (MQ=255)
                                                                                                                              cGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGTTGCCCGGCCTTGACCTGTTGGCCTGAGTCGAAGTGCAGGGATTTGACGATGCCTTCCACCTCCa                        >  2:196977/1‑150 (MQ=255)
                                                                                                                                  gccGACCTGGGCCTGGCCAAGGTCGACCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGTTGCCCGGCCTTGACCTGTTGGCCTGAGTCGAAGTGCAGGGATTTGACGATGCCTTCCACCTCCAGGCt                    >  1:1059022/3‑150 (MQ=255)
                                                                                                                                        cTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATcgtcgt                                                                                                         >  1:1103709/1‑59 (MQ=255)
                                                                                                                                        cTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATcgtcgt                                                                                                         <  2:1103709/59‑1 (MQ=255)
                                                                                                                                         ttGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGTTGCCCGGCCTTGACCTGTTGGCCTGAGTCGAAGTGCAGGGATTTGACGATGCCTTCCACCTCCAGGCTCAGATcc             <  2:1051740/150‑1 (MQ=255)
                                                                                                                                           ggCCTGGCCAAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCATTGAGCTGCAAGAGCAGTTGCCCGGCCTTGACCTGTTGGCATGAGTCGaa                                                        >  1:1187072/1‑105 (MQ=255)
                                                                                                                                           ggCCTGGCCAAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCATTGAGCTGCAAGAGCAGTTGCCCGGCCTTGACCTGTTGGCATGAGTCGaa                                                        <  2:1187072/105‑1 (MQ=255)
                                                                                                                                             ccAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGTTGCCCGGCCTTGACCTGTTGGCCTGAGTCGAAGTGCAGGGATTTGACGAt                                      >  2:834085/1‑121 (MQ=255)
                                                                                                                                             ccAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGTTGCCCGGCCTTGACCTGTTGGCCTGAGTCGAAGTGCAGGGATTTGACGAt                                      <  1:834085/121‑1 (MQ=255)
                                                                                                                                             ccAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGTTGCCCGGCCTTGACCTGTTGGCCTGAGTCGAAGTGCAGGGATTTGACGATGCCTTCCACCTCCAGGCTCAGATCCACGc         <  2:1314054/150‑1 (MQ=255)
                                                                                                                                                ggCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGTTGCCCGGCCTTGACCTGTTGGCCTGAGTCGAAGTGCAGGGATTTGACGATGCCTTCCACCTCCAGGCTCAGATCCACGCCCt      <  2:908830/150‑1 (MQ=255)
                                                                                                                                                    cAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGTTGCCCGGCCTTGACCTGTTGGCCTGAGTCGAAGTGCAGGGATTTGACGATGCCTTCCACCTCCAGGCTCAGATCCACGCCCTGCAg  <  1:853780/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TGCGTTTCTTGATCTTCTGCGCCTTAAGCTGCTCGACCACCGCCTGGTTGCGCCGGTACTGGGAGGAAAGGCGGTCGAACTCGCCGCGGGAGATGGCCGAATCGCCCACCAGCTGGCTGCCACGGCCGAAGTCGACCTTGGCCAGGCCCAGGTCGGCCAGGGCGGTACCGAGCAGGGCTGTTTCCTGATCGTCGTTGAGCTGCAAGAGCAGTTGCCCGGCCTTGACCTGTTGGCCTGAGTCGAAGTGCAGGGATTTGACGATGCCTTCCACCTCCAGGCTCAGATCCACGCCCTGCAG  >  NC_002947/1049044‑1049341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑