Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,145,454 A→G 9.5% intergenic (‑115/+26) hemO ← / ← PP_1006 heme oxygenase/heme receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,145,4540AG 9.5% 153.9 / 5.4 55intergenic (‑115/+26)hemO/PP_1006heme oxygenase/heme receptor
Reads supporting (aligned to +/- strand):  ref base A (27/22);  new base G (2/3);  total (30/25)
Fisher's exact test for biased strand distribution p-value = 6.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01

AGGCGCTGGGAGCGCAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGT‑AAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAGCTGCGCACCGAATCCCAC  >  NC_002947/1145305‑1145600
                                                                                                                                                      |                                                                                                                                                  
aGGCGCTGGGAGCGCAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGACGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGT‑AAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCa                                                                                                                                                    >  1:610091/1‑150 (MQ=255)
 ggCGCTGGGAGCGCAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGT‑AAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCaa                                                                                                                                                   >  2:1194928/1‑150 (MQ=255)
 ggCGCTGGGAGCGCAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTATAGATACAAGACGAAACAGCGGGAGCGAGACAGT‑AAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCaa                                                                                                                                                   <  2:649591/150‑1 (MQ=255)
  gcgcTGGGAGCGCAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACCGTCGAAGTAAGCAGGCCGCCTTGGT‑CCAGTACCGGCCTCTTCGCGGGCGTGCCCGCaaa                                                                                                                                                  >  1:1600385/1‑150 (MQ=255)
    gcTGGGAGCGCAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGT‑AAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAaga                                                                                                                                                <  2:890211/150‑1 (MQ=255)
     cTGGGAGCGCAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGT‑AAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAagag                                                                                                                                               <  1:1586992/150‑1 (MQ=255)
         gAGCGCAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGT‑AAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCg                                                                                                                                           <  1:1194928/150‑1 (MQ=255)
               aGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGT‑AAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTTGCGGGCACGCCCGCGAAGAGGCCGGTActg                                                                                                                                     <  2:1197013/150‑3 (MQ=38)
                                    ggggAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGT‑AAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATccc                                                                                                                >  2:611694/1‑150 (MQ=255)
                                    ggggAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGT‑AAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATccc                                                                                                                >  2:1415381/1‑150 (MQ=255)
                                             ttGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGT‑AAAAAAAGCCGGTATTTGCGTTGCCAGTCACGGCCTCTTCGCGGGCGTGCCAGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACAt                                                                                                       >  1:921705/1‑150 (MQ=255)
                                             ttGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGT‑AAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACAt                                                                                                       >  2:1338948/1‑150 (MQ=255)
                                             ttGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGT‑AAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACAt                                                                                                       >  2:1302274/1‑150 (MQ=255)
                                                gAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGT‑AAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGAACTTTAATAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGa                                                                                                    >  2:862682/1‑150 (MQ=255)
                                                             tAGATACAAGACGAAACAGCGGGAGCGAGACAGT‑AAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTgcg                                                                                       >  2:872046/1‑150 (MQ=255)
                                                             tAGATACAAGACGAAACAGCGGGAGCGAGACAGT‑AAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTgcg                                                                                       >  2:1221393/1‑150 (MQ=255)
                                                              aGATACAAGACGAAACAGCGGGAGCGAGACAGT‑AAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTgcgc                                                                                      >  1:186862/1‑150 (MQ=255)
                                                                   cAAGACGAAACAGCGGGAGCGAGACAGT‑AAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCgg                                                                                 <  1:1338948/150‑1 (MQ=255)
                                                                    aaGACGAAACAGCGGGAGCGAGACAGT‑AAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGAc                                                                                                                                   <  2:1496096/99‑1 (MQ=255)
                                                                    aaGACGAAACAGCGGGAGCGAGACAGT‑AAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGAc                                                                                                                                   >  1:1496096/1‑99 (MQ=255)
                                                                    aaGACGAAACAGCGGGAGCGAGACAGT‑AAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGc                                                                                >  1:635683/1‑150 (MQ=255)
                                                                     aGACGAAACAGCGGGAGCGAGACAGT‑AAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACAt                                                                                                       <  2:731270/126‑1 (MQ=255)
                                                                     aGACGAAACAGCGGGAGCGAGACAGT‑AAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACAt                                                                                                       >  1:731270/1‑126 (MQ=255)
                                                                         gAAACAGCGGGAGCGAGACAGT‑AAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGt                                                                           >  1:158111/1‑150 (MQ=255)
                                                                         gAAACAGCGGGAGCGAGACAGT‑AAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCACGCCAGCGAAGAGGCCg                                                                                                                                           >  2:425453/1‑86 (MQ=37)
                                                                         gAAACAGCGGGAGCGAGACAGT‑AAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCACGCCAGCGAAGAGGCCg                                                                                                                                           <  1:425453/86‑1 (MQ=37)
                                                                           aaCAGCGGGAGCGAGACAGT‑AAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCa                                                                         >  2:901504/1‑150 (MQ=255)
                                                                                 gggAGCGAGACAGT‑AAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGCTCCCGAATTTGCGCCCCGGCATGGTCAGGCGGt                                                                   <  1:862682/150‑1 (MQ=255)
                                                                                  ggAGCGAGACAGT‑AAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGGCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTc                                                                  >  2:1538892/1‑150 (MQ=255)
                                                                                     gCGAGACAGT‑AAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGAt                                                               <  2:158111/150‑1 (MQ=255)
                                                                                       gagaCAGT‑AAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCCCGGACGTGCCAGAAAAGAGGCCGGTACAGACGAAACGCCTCAGATATCCCCAACCACATAGATACCGGAGTTGCGCCACGGCATGGTGAGGAGGTCGATGt                                                             >  1:492352/1‑150 (MQ=255)
                                                                                         gaCAGT‑AAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTtg                                                           >  2:312439/1‑150 (MQ=255)
                                                                                           cAGT‑AAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTtggc                                                         <  1:1538892/150‑1 (MQ=255)
                                                                                            aGT‑AAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTtggct                                                        <  1:872046/150‑1 (MQ=255)
                                                                                             gT‑AAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTtggctg                                                       >  1:971951/1‑150 (MQ=255)
                                                                                              t‑AAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTtggctgg                                                      >  1:516960/1‑150 (MQ=255)
                                                                                                   aaaaGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGctggctgg                                                  <  1:611694/150‑1 (MQ=255)
                                                                                                     aaGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGt                                                <  1:1221393/150‑1 (MQ=255)
                                                                                                               ttGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAgccgcc                                      <  2:971951/150‑1 (MQ=255)
                                                                                                                       ccAGTACCGGCCTCTTCGCGGGCGAGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTg                              >  1:1119062/1‑150 (MQ=255)
                                                                                                                        cAGTACCGGCCTCTTCGAGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTgg                             >  1:132567/1‑150 (MQ=255)
                                                                                                                             ccGGCCTCTTTGCGGGCACGCCCGCGAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCg                                                                     <  2:193512/105‑1 (MQ=37)
                                                                                                                             ccGGCCTCTTTGCGGGCACGCCCGCGAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCg                                                                     >  1:193512/1‑105 (MQ=37)
                                                                                                                                gCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTa                     <  1:1415381/150‑1 (MQ=255)
                                                                                                                                gCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTa                     <  2:630320/150‑1 (MQ=255)
                                                                                                                                   tctTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACAtt                                                                                                      <  1:1018387/66‑1 (MQ=255)
                                                                                                                                   tctTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACAtt                                                                                                      >  2:1018387/1‑66 (MQ=255)
                                                                                                                                     ttCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAGCTgc                >  1:391815/1‑150 (MQ=255)
                                                                                                                                      tCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAgcc                                         <  2:76697/124‑1 (MQ=255)
                                                                                                                                      tCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAgcc                                         >  1:76697/1‑124 (MQ=255)
                                                                                                                                       cgcgGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAGCTgcgc              >  1:200657/1‑150 (MQ=255)
                                                                                                                                          gggCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTtgg                                                          <  1:21092/103‑1 (MQ=255)
                                                                                                                                          gggCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTtgg                                                          >  2:21092/1‑103 (MQ=255)
                                                                                                                                          gggCGTGCCAGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAGCTGCGCAcc           <  2:921705/150‑1 (MQ=255)
                                                                                                                                                  ccGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAGCTGCGCACCGAATCCCa   <  2:610091/150‑1 (MQ=255)
                                                                                                                                                  ccGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAGCTGCGCACCGAATCCCa   >  2:1203623/1‑150 (MQ=255)
                                                                                                                                                   cGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTc                                                                  >  2:497842/1‑86 (MQ=255)
                                                                                                                                                   cGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTc                                                                  <  1:497842/86‑1 (MQ=255)
                                                                                                                                                   cGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAGCTGCGCACCGAATCCCAc  <  2:200657/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                  
AGGCGCTGGGAGCGCAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGT‑AAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAGCTGCGCACCGAATCCCAC  >  NC_002947/1145305‑1145600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑